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The map label for this gene is nifH

Identifier: 45358416

GI number: 45358416

Start: 844090

End: 844917

Strand: Direct

Name: nifH

Synonym: MMP0853

Alternate gene names: 45358416

Gene position: 844090-844917 (Clockwise)

Preceding gene: 45358413

Following gene: 45358417

Centisome position: 50.81

GC content: 40.22

Gene sequence:

>828_bases
ATGGTAAGAAAAATCGCAATTTACGGAAAAGGTGGAATTGGAAAATCAACCACCACACAAAACACAGTAGCTGCAATGGC
TCACTTTCACGACAAAAAAGTATTCATTCACGGATGTGACCCAAAAGCAGACAGTACAAGGTTAATCTTGCACGGTAAAC
AGCAAGTAACAATGATGGATACATTAAGAGAAAAAGGGGAAGACGAATGTACTCCTGACAAAGTTATCGAAGTTGGTTTC
GGTGGCGTAAAATGTGTAGAATCAGGAGGACCTGAGCCAGGAGTAGGCTGTGCAGGTAGAGGTGTTATCACTGCAATTAC
ACTCATGGAACAGCACGGTGTTTACGAAGACGACCTTGACTTTGTATTCTTCGATGTTTTAGGGGACGTTGTATGTGGTG
GATTCGCAATGCCTGTAAGAGACGGTAAAGCAGACGAAATCTACGTTGTAGCATCTGGAGAAATGATGGCTCTTTACGCT
GCAAACAACATCTGTAAAGGAATGGTTAAATACGCAGAACAAAGCGGTGTAAGACTTGGAGGAATCATCTGTAACAGTAG
AAACGTAGATGGTGAGCTTGACTTATTACAAGAATTCTGTGACAAAATTGGAACACAATTAATCCACTTTGTTCCAAGAG
ACAACATCGTTCAAAAAGCAGAATTCCAGAAAAAAGCTGTTGTTGACTACGACGATACATGCAACCAAGCACTGGAATAC
AAAGAACTCGCAAGAAAAATCATTGAAAACGAAAACCTCGTAATTCCAACCCCAATGACAATGGACGAATTAGAAGAATT
AACTTCAAAATACGGATTCTTAGATTAA

Upstream 100 bases:

>100_bases
AATAATCTATAGCATAGTTCACCGGAAAGAAGCTTCCGTATAATAACTAAAAGATCGGAAATAAATTTCCGAAAGACAAC
AATATATAGAGGCCTAAAAA

Downstream 100 bases:

>100_bases
ATAAAGTAAATTACTTACCTTAAATTTTTAAGAGGGCAAAAAATGAAAATGATTAGAGCAGTAGTCAGACCAAGTAAAGC
AGAAGAAGTTGTAGATGCAC

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MVRKIAIYGKGGIGKSTTTQNTVAAMAHFHDKKVFIHGCDPKADSTRLILHGKQQVTMMDTLREKGEDECTPDKVIEVGF
GGVKCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDDLDFVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYA
ANNICKGMVKYAEQSGVRLGGIICNSRNVDGELDLLQEFCDKIGTQLIHFVPRDNIVQKAEFQKKAVVDYDDTCNQALEY
KELARKIIENENLVIPTPMTMDELEELTSKYGFLD

Sequences:

>Translated_275_residues
MVRKIAIYGKGGIGKSTTTQNTVAAMAHFHDKKVFIHGCDPKADSTRLILHGKQQVTMMDTLREKGEDECTPDKVIEVGF
GGVKCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDDLDFVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYA
ANNICKGMVKYAEQSGVRLGGIICNSRNVDGELDLLQEFCDKIGTQLIHFVPRDNIVQKAEFQKKAVVDYDDTCNQALEY
KELARKIIENENLVIPTPMTMDELEELTSKYGFLD
>Mature_275_residues
MVRKIAIYGKGGIGKSTTTQNTVAAMAHFHDKKVFIHGCDPKADSTRLILHGKQQVTMMDTLREKGEDECTPDKVIEVGF
GGVKCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDDLDFVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYA
ANNICKGMVKYAEQSGVRLGGIICNSRNVDGELDLLQEFCDKIGTQLIHFVPRDNIVQKAEFQKKAVVDYDDTCNQALEY
KELARKIIENENLVIPTPMTMDELEELTSKYGFLD

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH_METMP (Q50218)

Other databases:

- EMBL:   U75887
- EMBL:   BX950229
- PIR:   T10090
- RefSeq:   NP_987973.1
- ProteinModelPortal:   Q50218
- SMR:   Q50218
- GeneID:   2762307
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0853
- NMPDR:   fig|267377.1.peg.853
- HOGENOM:   HBG488165
- OMA:   KYAEQSG
- ProtClustDB:   PRK13233
- BioCyc:   MMAR267377:MMP0853-MONOMER
- BRENDA:   1.18.6.1
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 30146; Mature: 30146

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKIAIYGKGGIGKSTTTQNTVAAMAHFHDKKVFIHGCDPKADSTRLILHGKQQVTMMD
CCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHHH
TLREKGEDECTPDKVIEVGFGGVKCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDDLD
HHHHCCCCCCCHHHEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHH
FVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYAANNICKGMVKYAEQSGVRLG
HHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCEEE
GIICNSRNVDGELDLLQEFCDKIGTQLIHFVPRDNIVQKAEFQKKAVVDYDDTCNQALEY
EEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
KELARKIIENENLVIPTPMTMDELEELTSKYGFLD
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVRKIAIYGKGGIGKSTTTQNTVAAMAHFHDKKVFIHGCDPKADSTRLILHGKQQVTMMD
CCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHHH
TLREKGEDECTPDKVIEVGFGGVKCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDDLD
HHHHCCCCCCCHHHEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHH
FVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYAANNICKGMVKYAEQSGVRLG
HHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCEEE
GIICNSRNVDGELDLLQEFCDKIGTQLIHFVPRDNIVQKAEFQKKAVVDYDDTCNQALEY
EEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
KELARKIIENENLVIPTPMTMDELEELTSKYGFLD
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592322