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The map label for this gene is mcrB [H]

Identifier: 45358318

GI number: 45358318

Start: 746442

End: 748256

Strand: Direct

Name: mcrB [H]

Synonym: MMP0755

Alternate gene names: 45358318

Gene position: 746442-748256 (Clockwise)

Preceding gene: 45358316

Following gene: 45358319

Centisome position: 44.94

GC content: 32.34

Gene sequence:

>1815_bases
ATGAATGGCCATACGAATGCAGTATATTTTTTAAATTGTTCAGACGATGAAGAGAATTTTAATCTTTGTTTGGAGCATGA
TGTTGCAGGTTTTACAAGCACAGGCTATGAAATAGGGGATTTAGCATATATTATACTTAAGAAAGGTAGAATATGGTATC
TAGGTGGTAAGGGTGTTCTTGAAAAAGAAACAGATCTAAAACCCTGGAAAGATGCAGCAAGATACAATTCGGTATTCAAA
ATTAACTGGGATGTTTGTAATCTGACTCCAATCACTGAAGGTTTACAACATATTTATGATAATTTTGGATTGGCAATACA
GGGGAAAAAGGATTTAAGAAATTTTTTAGGAAAAGGGGATGAAATAGTTTCTTATTTGGACAGTGTTATCTCTAAAAATC
GTACAACAAGACGCAATGTTGTCAGGAGGGATACTATAATCAATAATCCGATCAATATAGGCGAAGATGAAACTGATAAG
GAATACGCCCTGAATACAATTCTTTATGGCCCTCCAGGAACAGGTAAAACCTACCGGGTAATTGAGGAGGCTGTAGCGAT
TATTGATGGGAAAATACCTAAGGGCAGGGTAAATCTCGTTAAAAGATACAAGGAATTAAAAGAAAGGGGACAGATTGAAT
TTATAACATTTCACCAGTCTTACTGTTACGAAGACTTTATTGAAGGAATTAAACCTGTTTTAGAAAATTCGGACGAAAAT
TCAATCGAATACAAAATTGAAGATGGAATTTTTAAAAAAATGGTAAATAAAGCTATCGAAGTTAAAAGTTCAAGTAATTT
CGATGAAAAATATGCTAAATTTGTGGAAGATGTCCTTGAACAGGATGGAATTGAATTAGAAACGTTGGCCCAAAAAAAAC
CATTTAGGGTTAGGATTAATAGCAATGAAACCGCGGTTGCAATACCTAAAACAGAAGCTGCTACAAGATTGAGTGTTACT
AAAGAAATGTTACGAGATTATATTGTTAATGATAATATTCAGGGGTGGAAACCTTATACTCCTGCAATTGGTGAGTATTT
AAAAAATAAATATGGTATCACTGTTGAAAATACGGATAATACTAATAAAAAGTTCGTTTTGATAATCGACGAAATAAATC
GTGGAAACATTTCTAAAATATTTGGAGAATTAATTACATTGATTGAACCAGATAAACGAATTGGGGAATTAAATGAACTT
ACTGTAACACTTCCATACTCTAAAAAGGATTTTGGATTACCTTCCAATTTATATATTCTAGGAACAATGAATACTGCCGA
CAGATCAATTGCATTAGTTGATACTGCCCTTAGACGACGATTTGAATTTAACGAAATGATGCCTGATGTTAAAAAATTAG
GATTTGATATAAAAGGTATTCAAATAGATATAATGCTCGAATCGATAAATAAGAGAATTGAGTATTTCAGAGGAAGGGAT
CATACCATAGGACATGCTTACTTCATGGATCTTATCAATGAGACCAATGAACAAGTACGATTTAAAAAATTACAGGAAAT
TTTCCAATATAAAGTAATTCCACTTTTACAGGAATATTTCTACGAAAATTGGGAAACAATTCAGTATATTCTTGGGGATC
ATCCTGATCAATTAAAACGATTTAAAGATAATCCTGAGTTAACAATTGTCCAAAGAGATAATACCAATTTAAGAAACTTA
TTTGGGGCGGATATGGGCGATTCGGAAAATCCAAATGGATATTTTATCAATCCACGATTAAAAGATGGAGAAATGCATCC
AAATGCATTTATTAAGATATATAATTCCAAGGTAAACGAATCTTTGGAGTACTAA

Upstream 100 bases:

>100_bases
TTTATTTTTGAACATAAATAAAAAGTGTTTTATTGGGGCGGTTACAGAATAGTTGAATTATAACTTGATTTTATATGATT
ATTTAGAATAGGGGGATTAG

Downstream 100 bases:

>100_bases
AATGAACAATTTAAAAGATATAACTCTAAATGAGTTTCAATACCTCATAAAAGGGCTTAATGACGAACCTCATGATCGAG
GTTTTGTGATTAATAAACAT

Product: ATP/GTP-binding motif-containing protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MNGHTNAVYFLNCSDDEENFNLCLEHDVAGFTSTGYEIGDLAYIILKKGRIWYLGGKGVLEKETDLKPWKDAARYNSVFK
INWDVCNLTPITEGLQHIYDNFGLAIQGKKDLRNFLGKGDEIVSYLDSVISKNRTTRRNVVRRDTIINNPINIGEDETDK
EYALNTILYGPPGTGKTYRVIEEAVAIIDGKIPKGRVNLVKRYKELKERGQIEFITFHQSYCYEDFIEGIKPVLENSDEN
SIEYKIEDGIFKKMVNKAIEVKSSSNFDEKYAKFVEDVLEQDGIELETLAQKKPFRVRINSNETAVAIPKTEAATRLSVT
KEMLRDYIVNDNIQGWKPYTPAIGEYLKNKYGITVENTDNTNKKFVLIIDEINRGNISKIFGELITLIEPDKRIGELNEL
TVTLPYSKKDFGLPSNLYILGTMNTADRSIALVDTALRRRFEFNEMMPDVKKLGFDIKGIQIDIMLESINKRIEYFRGRD
HTIGHAYFMDLINETNEQVRFKKLQEIFQYKVIPLLQEYFYENWETIQYILGDHPDQLKRFKDNPELTIVQRDNTNLRNL
FGADMGDSENPNGYFINPRLKDGEMHPNAFIKIYNSKVNESLEY

Sequences:

>Translated_604_residues
MNGHTNAVYFLNCSDDEENFNLCLEHDVAGFTSTGYEIGDLAYIILKKGRIWYLGGKGVLEKETDLKPWKDAARYNSVFK
INWDVCNLTPITEGLQHIYDNFGLAIQGKKDLRNFLGKGDEIVSYLDSVISKNRTTRRNVVRRDTIINNPINIGEDETDK
EYALNTILYGPPGTGKTYRVIEEAVAIIDGKIPKGRVNLVKRYKELKERGQIEFITFHQSYCYEDFIEGIKPVLENSDEN
SIEYKIEDGIFKKMVNKAIEVKSSSNFDEKYAKFVEDVLEQDGIELETLAQKKPFRVRINSNETAVAIPKTEAATRLSVT
KEMLRDYIVNDNIQGWKPYTPAIGEYLKNKYGITVENTDNTNKKFVLIIDEINRGNISKIFGELITLIEPDKRIGELNEL
TVTLPYSKKDFGLPSNLYILGTMNTADRSIALVDTALRRRFEFNEMMPDVKKLGFDIKGIQIDIMLESINKRIEYFRGRD
HTIGHAYFMDLINETNEQVRFKKLQEIFQYKVIPLLQEYFYENWETIQYILGDHPDQLKRFKDNPELTIVQRDNTNLRNL
FGADMGDSENPNGYFINPRLKDGEMHPNAFIKIYNSKVNESLEY
>Mature_604_residues
MNGHTNAVYFLNCSDDEENFNLCLEHDVAGFTSTGYEIGDLAYIILKKGRIWYLGGKGVLEKETDLKPWKDAARYNSVFK
INWDVCNLTPITEGLQHIYDNFGLAIQGKKDLRNFLGKGDEIVSYLDSVISKNRTTRRNVVRRDTIINNPINIGEDETDK
EYALNTILYGPPGTGKTYRVIEEAVAIIDGKIPKGRVNLVKRYKELKERGQIEFITFHQSYCYEDFIEGIKPVLENSDEN
SIEYKIEDGIFKKMVNKAIEVKSSSNFDEKYAKFVEDVLEQDGIELETLAQKKPFRVRINSNETAVAIPKTEAATRLSVT
KEMLRDYIVNDNIQGWKPYTPAIGEYLKNKYGITVENTDNTNKKFVLIIDEINRGNISKIFGELITLIEPDKRIGELNEL
TVTLPYSKKDFGLPSNLYILGTMNTADRSIALVDTALRRRFEFNEMMPDVKKLGFDIKGIQIDIMLESINKRIEYFRGRD
HTIGHAYFMDLINETNEQVRFKKLQEIFQYKVIPLLQEYFYENWETIQYILGDHPDQLKRFKDNPELTIVQRDNTNLRNL
FGADMGDSENPNGYFINPRLKDGEMHPNAFIKIYNSKVNESLEY

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=243, Percent_Identity=34.9794238683128, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 69802; Mature: 69802

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGHTNAVYFLNCSDDEENFNLCLEHDVAGFTSTGYEIGDLAYIILKKGRIWYLGGKGVL
CCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHCEEEEEEECCEEEEECCCCCC
EKETDLKPWKDAARYNSVFKINWDVCNLTPITEGLQHIYDNFGLAIQGKKDLRNFLGKGD
CCCCCCCCHHHHHHCCCEEEEECCEEECCHHHHHHHHHHHCCCEEEECHHHHHHHHCCCH
EIVSYLDSVISKNRTTRRNVVRRDTIINNPINIGEDETDKEYALNTILYGPPGTGKTYRV
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCCCCCCHHHH
IEEAVAIIDGKIPKGRVNLVKRYKELKERGQIEFITFHQSYCYEDFIEGIKPVLENSDEN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCC
SIEYKIEDGIFKKMVNKAIEVKSSSNFDEKYAKFVEDVLEQDGIELETLAQKKPFRVRIN
CEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHCCCCEEEEEC
SNETAVAIPKTEAATRLSVTKEMLRDYIVNDNIQGWKPYTPAIGEYLKNKYGITVENTDN
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCCC
TNKKFVLIIDEINRGNISKIFGELITLIEPDKRIGELNELTVTLPYSKKDFGLPSNLYIL
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCCCCCEEEE
GTMNTADRSIALVDTALRRRFEFNEMMPDVKKLGFDIKGIQIDIMLESINKRIEYFRGRD
EECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCC
HTIGHAYFMDLINETNEQVRFKKLQEIFQYKVIPLLQEYFYENWETIQYILGDHPDQLKR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
FKDNPELTIVQRDNTNLRNLFGADMGDSENPNGYFINPRLKDGEMHPNAFIKIYNSKVNE
CCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCHHHH
SLEY
CCCC
>Mature Secondary Structure
MNGHTNAVYFLNCSDDEENFNLCLEHDVAGFTSTGYEIGDLAYIILKKGRIWYLGGKGVL
CCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHCEEEEEEECCEEEEECCCCCC
EKETDLKPWKDAARYNSVFKINWDVCNLTPITEGLQHIYDNFGLAIQGKKDLRNFLGKGD
CCCCCCCCHHHHHHCCCEEEEECCEEECCHHHHHHHHHHHCCCEEEECHHHHHHHHCCCH
EIVSYLDSVISKNRTTRRNVVRRDTIINNPINIGEDETDKEYALNTILYGPPGTGKTYRV
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCCCCCCHHHH
IEEAVAIIDGKIPKGRVNLVKRYKELKERGQIEFITFHQSYCYEDFIEGIKPVLENSDEN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCC
SIEYKIEDGIFKKMVNKAIEVKSSSNFDEKYAKFVEDVLEQDGIELETLAQKKPFRVRIN
CEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHCCCCEEEEEC
SNETAVAIPKTEAATRLSVTKEMLRDYIVNDNIQGWKPYTPAIGEYLKNKYGITVENTDN
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCCC
TNKKFVLIIDEINRGNISKIFGELITLIEPDKRIGELNELTVTLPYSKKDFGLPSNLYIL
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCCCCCEEEE
GTMNTADRSIALVDTALRRRFEFNEMMPDVKKLGFDIKGIQIDIMLESINKRIEYFRGRD
EECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCC
HTIGHAYFMDLINETNEQVRFKKLQEIFQYKVIPLLQEYFYENWETIQYILGDHPDQLKR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
FKDNPELTIVQRDNTNLRNLFGADMGDSENPNGYFINPRLKDGEMHPNAFIKIYNSKVNE
CCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCHHHH
SLEY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]