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The map label for this gene is mjaIIIM [H]

Identifier: 45358298

GI number: 45358298

Start: 727879

End: 728799

Strand: Direct

Name: mjaIIIM [H]

Synonym: MMP0735

Alternate gene names: 45358298

Gene position: 727879-728799 (Clockwise)

Preceding gene: 45358297

Following gene: 45358299

Centisome position: 43.82

GC content: 31.49

Gene sequence:

>921_bases
ATGCACCGAAAAGCGAAACCTTTTTTAAAATGGGCTGGCGGTAAAAGAAGACTTTTAACCCAGTTTGAAGACCACTATCC
GGAAGGATTGAAGAATGGAAAGATTAAAAAGTACGTTGAGCCATTTTTAGGTGGTGGTGCAGTTTATCTGAGCCTCCAAA
GTAAATATAAATTTAAAAAAGTGGTTTTGAATGATATAAATCACGAATTAATGCTTTCTTATAAAACTGTTCAAAATAAC
ATTGATGAATTAATTTCTATTTTGAAACCTATTGAAGAAAATTTTAACAAAATGAACTTCGAATCTCAAAAAATGCAATA
TTACAAAATAAGAAATGAATACAACATTGAAAAATCAAACATTGATAAAAATAAATCTGAAAATATCATTGAAAATGTCG
CGAGATTTATTTTTTTAAATAAAACCTGTTTTAATGGCCTTTATAGGCTGAATAAGAAAGGAATGTTCAACGTTCCATTT
GGAAGGTATCTTCGTCCAAGAATATTTGATGAACCAACCCTTAGGGGAGTAAACAAGGCTTTAAAAGGCGTAAAATTACT
GTGTGATGACTATAAAAACGTTGAAAAGCATATATCTATTGACGAAGAAACTTTTGTATATATCGATCCACCTTACAGGC
CCCTTCCAGAAACCGTTAGTTTTACATCTTATTCTAAAGAGGATTTCCTTGAAAAAGATCAGGTAGGCTTGAGCAACTGG
TTTAAATATCTGGACAAAAAAGGCGCGTATTTAATGCTCAGTAATTCTGACCCGACAAATACAAATCCAAAAGACCGATT
TTTTGAAGAACACTATGGCGGATTTAAAATCGATAAAGTTTGTGCTGGAAGAATAATAAATTCAAGAGTCAACGGAAGGC
GAAAAATAACAGAATTAGTAATTAAAAACTATCCAAAATAA

Upstream 100 bases:

>100_bases
ATATAGATCTAATTGACAGTTTTTGACCAAATAACGCAACGAAAAGATCCAGTACCGAAAAATATACATATATTGTCTTT
ATACTCTTTTCTGATATGTT

Downstream 100 bases:

>100_bases
TATTTAAGGGCAGTAATGGGATATATGTTAATCGTGTTCTTCAGAGAGCCATTCTTCCCATTTTATTAGTTTATATCCGT
CAACCATAACGGCATTGCGG

Product: N-6 adenine-specific DNA methylase:N6 adenine-specific DNA methyltransferase, D12 class

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MHRKAKPFLKWAGGKRRLLTQFEDHYPEGLKNGKIKKYVEPFLGGGAVYLSLQSKYKFKKVVLNDINHELMLSYKTVQNN
IDELISILKPIEENFNKMNFESQKMQYYKIRNEYNIEKSNIDKNKSENIIENVARFIFLNKTCFNGLYRLNKKGMFNVPF
GRYLRPRIFDEPTLRGVNKALKGVKLLCDDYKNVEKHISIDEETFVYIDPPYRPLPETVSFTSYSKEDFLEKDQVGLSNW
FKYLDKKGAYLMLSNSDPTNTNPKDRFFEEHYGGFKIDKVCAGRIINSRVNGRRKITELVIKNYPK

Sequences:

>Translated_306_residues
MHRKAKPFLKWAGGKRRLLTQFEDHYPEGLKNGKIKKYVEPFLGGGAVYLSLQSKYKFKKVVLNDINHELMLSYKTVQNN
IDELISILKPIEENFNKMNFESQKMQYYKIRNEYNIEKSNIDKNKSENIIENVARFIFLNKTCFNGLYRLNKKGMFNVPF
GRYLRPRIFDEPTLRGVNKALKGVKLLCDDYKNVEKHISIDEETFVYIDPPYRPLPETVSFTSYSKEDFLEKDQVGLSNW
FKYLDKKGAYLMLSNSDPTNTNPKDRFFEEHYGGFKIDKVCAGRIINSRVNGRRKITELVIKNYPK
>Mature_306_residues
MHRKAKPFLKWAGGKRRLLTQFEDHYPEGLKNGKIKKYVEPFLGGGAVYLSLQSKYKFKKVVLNDINHELMLSYKTVQNN
IDELISILKPIEENFNKMNFESQKMQYYKIRNEYNIEKSNIDKNKSENIIENVARFIFLNKTCFNGLYRLNKKGMFNVPF
GRYLRPRIFDEPTLRGVNKALKGVKLLCDDYKNVEKHISIDEETFVYIDPPYRPLPETVSFTSYSKEDFLEKDQVGLSNW
FKYLDKKGAYLMLSNSDPTNTNPKDRFFEEHYGGFKIDKVCAGRIINSRVNGRRKITELVIKNYPK

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=301, Percent_Identity=31.8936877076412, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 36040; Mature: 36040

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRKAKPFLKWAGGKRRLLTQFEDHYPEGLKNGKIKKYVEPFLGGGAVYLSLQSKYKFKK
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHH
VVLNDINHELMLSYKTVQNNIDELISILKPIEENFNKMNFESQKMQYYKIRNEYNIEKSN
HHHHHCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHC
IDKNKSENIIENVARFIFLNKTCFNGLYRLNKKGMFNVPFGRYLRPRIFDEPTLRGVNKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCHHHHHHHHH
LKGVKLLCDDYKNVEKHISIDEETFVYIDPPYRPLPETVSFTSYSKEDFLEKDQVGLSNW
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
FKYLDKKGAYLMLSNSDPTNTNPKDRFFEEHYGGFKIDKVCAGRIINSRVNGRRKITELV
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
IKNYPK
HHCCCC
>Mature Secondary Structure
MHRKAKPFLKWAGGKRRLLTQFEDHYPEGLKNGKIKKYVEPFLGGGAVYLSLQSKYKFKK
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHH
VVLNDINHELMLSYKTVQNNIDELISILKPIEENFNKMNFESQKMQYYKIRNEYNIEKSN
HHHHHCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHC
IDKNKSENIIENVARFIFLNKTCFNGLYRLNKKGMFNVPFGRYLRPRIFDEPTLRGVNKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCHHHHHHHHH
LKGVKLLCDDYKNVEKHISIDEETFVYIDPPYRPLPETVSFTSYSKEDFLEKDQVGLSNW
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
FKYLDKKGAYLMLSNSDPTNTNPKDRFFEEHYGGFKIDKVCAGRIINSRVNGRRKITELV
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
IKNYPK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]