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The map label for this gene is hisJ [H]

Identifier: 45358275

GI number: 45358275

Start: 705074

End: 705820

Strand: Direct

Name: hisJ [H]

Synonym: MMP0712

Alternate gene names: 45358275

Gene position: 705074-705820 (Clockwise)

Preceding gene: 45358274

Following gene: 45358276

Centisome position: 42.45

GC content: 31.06

Gene sequence:

>747_bases
TTGAAAAAAGCTACCTTTATATTACTATTTAGCATTTTAGTAATAACTTCAGCATGCACGGATACCAAAGAGTATTCTGA
ATCAGGTACGCTGACCGTTGGAATATATCCTTACATGCCCCCGTACCAATATTATGAAAACGATAAATTGGAAGGTTTTG
AAATAGAATTGGTTTCTGAAATTGCTAGACGAATGAATAAACAAATTGAATTTGTTGAAACTGATGACAAATATGGAAAT
TATGTTGCAGCTCGTTCTGGAACTGTTGATGTTTCAATTTCTGCAATTACGAAAACTGCTTCAAGGGAAAAAGATTCAAT
ATATTCTGAACTCTACATGAATTCTTATTCGTCCCTCGTATGCGTTGATTCAAAATATTCTCGTTTGGCAGATTTTGAGG
GTAAAAAAATAGGGATTGTTGATGACACCGTTTATGTTGACCATGCAGAATCCTACAAAAAAAGTGTTGACTTTGAAATC
GTAAAATATTATAATTATTATGATTTAAATTCAGCATTATTGGACGGTGAGGTTGATGGGGCCCTTGCTGACTATTTCAC
ATGTAAATATTTTATGAAAAATGACGATGATTTAACGATAATTGAAAATACTGATATTCACTACTTTGGAATGGTTTCTT
CAATAGGAAATGAAGATTTAATAAATAATATAGATGAAGTTTTAATGAGTATGTATAATGATGGAAGTTACGAAAAATTA
CGGCTAAAATACTTTGAAATTGAATAA

Upstream 100 bases:

>100_bases
AAAAAGTGGATTTAATTATATTGATATATCCGAAAATTATATTTAATATTAATGATAATTAATAATTAAAATATTATATC
CCCCGCTAAAAGGTGAATTT

Downstream 100 bases:

>100_bases
ACTTAAATAAATTCACTTATTTTAAATTTTTTCAGGTTTTGCGTTAATTTTGATATAAATATGTCTAAAAAAATCAATTA
ATAATTAATATATATTGATT

Product: amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP; p29 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKKATFILLFSILVITSACTDTKEYSESGTLTVGIYPYMPPYQYYENDKLEGFEIELVSEIARRMNKQIEFVETDDKYGN
YVAARSGTVDVSISAITKTASREKDSIYSELYMNSYSSLVCVDSKYSRLADFEGKKIGIVDDTVYVDHAESYKKSVDFEI
VKYYNYYDLNSALLDGEVDGALADYFTCKYFMKNDDDLTIIENTDIHYFGMVSSIGNEDLINNIDEVLMSMYNDGSYEKL
RLKYFEIE

Sequences:

>Translated_248_residues
MKKATFILLFSILVITSACTDTKEYSESGTLTVGIYPYMPPYQYYENDKLEGFEIELVSEIARRMNKQIEFVETDDKYGN
YVAARSGTVDVSISAITKTASREKDSIYSELYMNSYSSLVCVDSKYSRLADFEGKKIGIVDDTVYVDHAESYKKSVDFEI
VKYYNYYDLNSALLDGEVDGALADYFTCKYFMKNDDDLTIIENTDIHYFGMVSSIGNEDLINNIDEVLMSMYNDGSYEKL
RLKYFEIE
>Mature_248_residues
MKKATFILLFSILVITSACTDTKEYSESGTLTVGIYPYMPPYQYYENDKLEGFEIELVSEIARRMNKQIEFVETDDKYGN
YVAARSGTVDVSISAITKTASREKDSIYSELYMNSYSSLVCVDSKYSRLADFEGKKIGIVDDTVYVDHAESYKKSVDFEI
VKYYNYYDLNSALLDGEVDGALADYFTCKYFMKNDDDLTIIENTDIHYFGMVSSIGNEDLINNIDEVLMSMYNDGSYEKL
RLKYFEIE

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=22.707423580786, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=27.7056277056277, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787088, Length=254, Percent_Identity=20.8661417322835, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=24.2424242424242, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=24.2307692307692, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28500; Mature: 28500

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKATFILLFSILVITSACTDTKEYSESGTLTVGIYPYMPPYQYYENDKLEGFEIELVSE
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCHHHCCCCCCCCEEHHHHHH
IARRMNKQIEFVETDDKYGNYVAARSGTVDVSISAITKTASREKDSIYSELYMNSYSSLV
HHHHHCCCEEEEECCCCCCCEEEECCCEEEEEEHHHHHHHCCCHHHHHHHHHHCCCCCEE
CVDSKYSRLADFEGKKIGIVDDTVYVDHAESYKKSVDFEIVKYYNYYDLNSALLDGEVDG
EECCHHHHHHCCCCCEEEEEECEEEEECHHHHHHCCCEEEEEEEEEEECCCEEECCCCCC
ALADYFTCKYFMKNDDDLTIIENTDIHYFGMVSSIGNEDLINNIDEVLMSMYNDGSYEKL
HHHHHHEEEEEECCCCCEEEEECCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
RLKYFEIE
EEEEEECC
>Mature Secondary Structure
MKKATFILLFSILVITSACTDTKEYSESGTLTVGIYPYMPPYQYYENDKLEGFEIELVSE
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCHHHCCCCCCCCEEHHHHHH
IARRMNKQIEFVETDDKYGNYVAARSGTVDVSISAITKTASREKDSIYSELYMNSYSSLV
HHHHHCCCEEEEECCCCCCCEEEECCCEEEEEEHHHHHHHCCCHHHHHHHHHHCCCCCEE
CVDSKYSRLADFEGKKIGIVDDTVYVDHAESYKKSVDFEIVKYYNYYDLNSALLDGEVDG
EECCHHHHHHCCCCCEEEEEECEEEEECHHHHHHCCCEEEEEEEEEEECCCEEECCCCCC
ALADYFTCKYFMKNDDDLTIIENTDIHYFGMVSSIGNEDLINNIDEVLMSMYNDGSYEKL
HHHHHHEEEEEECCCCCEEEEECCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
RLKYFEIE
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9192026; 9395059; 10688204 [H]