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The map label for this gene is mptN

Identifier: 45358265

GI number: 45358265

Start: 692462

End: 693340

Strand: Direct

Name: mptN

Synonym: MMP0702

Alternate gene names: 45358265

Gene position: 692462-693340 (Clockwise)

Preceding gene: 45358264

Following gene: 45358271

Centisome position: 41.69

GC content: 34.81

Gene sequence:

>879_bases
ATGAGAATGGGAATAATCTCTGAAGAAAGGGACTGGGTAACCGATGAGTTGAAGTCCAAAATGGAAAAAAATGACATCGA
CCCAGTTATTATACAGCCCTCAAAAATAATTTCTTATATCGAATCGGAAGTTAAGTTTGAACAGAATAATCGGAGTATAC
TTGACTTAAAATGCGCGTTTGTTAGAAACATCGGTGAAGGAGTCGAAATGTTTCACAGGTTTGACATGTTAAAATACCTT
GAAAATTACGTACCCATAATAAACCCGATGGATGGAATTGAAAATGCAGGAAATAAATTCAGGACATCATTTTTAATGGA
AGTTCACAAAATTCCTCATCCAAAAACAATCGTTGCAGAAGATGTAAATAAAGCGTTAATCGCAGCAGATAAATTTGAAG
ATGTTGTTTTAAAACCTCTTTTTGGAAATCAAGGAAAAGGCCTTGTAAGGGTTAAGGGTAGATCAACCGTTGCAAAATTA
AAAGCGTTAAATACTTTTAAAAGCACTCACGGCGTAATTTATATGCAAGAATTTGTAAACAATCCAAATAACGTTTATAG
GGACATTCGAGCATTTGTAGTTGGGGATAAGGTAATTTCTGCAATGTATAGAACGTCTGACAACTGGATTACAAATATTC
ACCAGAATGGTGTTCCAGAAAAGTGCGAAATAACTGAAGAATTAAGTAAAATTGTGCTTGCTGCAAAAGATGCAGTAGGG
CTCGTTTATGCCGGTGTAGATATACTCGAAAGCAGTGATGGGTTAAAAGTTATCGAAGTAAATGCATGTCCTTCATGGGA
AGGACTTTCAAGAATTTCTGAAGTAGATATCGCTCAAAACTTAATTGATGAAGCTTTAAATTATGCAAAAGAGTATTAA

Upstream 100 bases:

>100_bases
AAGGGCATTCCACAATCCGACGCCGCTAAACGTTAAAATTAAAAAAATGTTTTCAGATTTTTAATGTAAATATTATTTTT
ATAATTTTTATGGTGTTAAT

Downstream 100 bases:

>100_bases
AAATTTAATTTTGAAGATATATCTTTTTCATTTTTTTAATGATTTCAGAGGAATTTAAATTTTCTTTTTGAATGTTGAGG
AGTTTTTCATCTATTTTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRMGIISEERDWVTDELKSKMEKNDIDPVIIQPSKIISYIESEVKFEQNNRSILDLKCAFVRNIGEGVEMFHRFDMLKYL
ENYVPIINPMDGIENAGNKFRTSFLMEVHKIPHPKTIVAEDVNKALIAADKFEDVVLKPLFGNQGKGLVRVKGRSTVAKL
KALNTFKSTHGVIYMQEFVNNPNNVYRDIRAFVVGDKVISAMYRTSDNWITNIHQNGVPEKCEITEELSKIVLAAKDAVG
LVYAGVDILESSDGLKVIEVNACPSWEGLSRISEVDIAQNLIDEALNYAKEY

Sequences:

>Translated_292_residues
MRMGIISEERDWVTDELKSKMEKNDIDPVIIQPSKIISYIESEVKFEQNNRSILDLKCAFVRNIGEGVEMFHRFDMLKYL
ENYVPIINPMDGIENAGNKFRTSFLMEVHKIPHPKTIVAEDVNKALIAADKFEDVVLKPLFGNQGKGLVRVKGRSTVAKL
KALNTFKSTHGVIYMQEFVNNPNNVYRDIRAFVVGDKVISAMYRTSDNWITNIHQNGVPEKCEITEELSKIVLAAKDAVG
LVYAGVDILESSDGLKVIEVNACPSWEGLSRISEVDIAQNLIDEALNYAKEY
>Mature_292_residues
MRMGIISEERDWVTDELKSKMEKNDIDPVIIQPSKIISYIESEVKFEQNNRSILDLKCAFVRNIGEGVEMFHRFDMLKYL
ENYVPIINPMDGIENAGNKFRTSFLMEVHKIPHPKTIVAEDVNKALIAADKFEDVVLKPLFGNQGKGLVRVKGRSTVAKL
KALNTFKSTHGVIYMQEFVNNPNNVYRDIRAFVVGDKVISAMYRTSDNWITNIHQNGVPEKCEITEELSKIVLAAKDAVG
LVYAGVDILESSDGLKVIEVNACPSWEGLSRISEVDIAQNLIDEALNYAKEY

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1787076, Length=220, Percent_Identity=30.4545454545455, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MPTN_METMP (Q6LZC7)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_987822.1
- ProteinModelPortal:   Q6LZC7
- SMR:   Q6LZC7
- GeneID:   2762372
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0702
- NMPDR:   fig|267377.1.peg.702
- HOGENOM:   HBG679591
- OMA:   ITNIHQN
- ProtClustDB:   CLSK876269
- BioCyc:   MMAR267377:MMP0702-MONOMER
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 33016; Mature: 33016

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMGIISEERDWVTDELKSKMEKNDIDPVIIQPSKIISYIESEVKFEQNNRSILDLKCAF
CCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCCCEEEEHHHHH
VRNIGEGVEMFHRFDMLKYLENYVPIINPMDGIENAGNKFRTSFLMEVHKIPHPKTIVAE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
DVNKALIAADKFEDVVLKPLFGNQGKGLVRVKGRSTVAKLKALNTFKSTHGVIYMQEFVN
HHHHHHHHHHHHHHHHEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEHHHHC
NPNNVYRDIRAFVVGDKVISAMYRTSDNWITNIHQNGVPEKCEITEELSKIVLAAKDAVG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LVYAGVDILESSDGLKVIEVNACPSWEGLSRISEVDIAQNLIDEALNYAKEY
HHHHCHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRMGIISEERDWVTDELKSKMEKNDIDPVIIQPSKIISYIESEVKFEQNNRSILDLKCAF
CCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCCCEEEEHHHHH
VRNIGEGVEMFHRFDMLKYLENYVPIINPMDGIENAGNKFRTSFLMEVHKIPHPKTIVAE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
DVNKALIAADKFEDVVLKPLFGNQGKGLVRVKGRSTVAKLKALNTFKSTHGVIYMQEFVN
HHHHHHHHHHHHHHHHEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEHHHHC
NPNNVYRDIRAFVVGDKVISAMYRTSDNWITNIHQNGVPEKCEITEELSKIVLAAKDAVG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LVYAGVDILESSDGLKVIEVNACPSWEGLSRISEVDIAQNLIDEALNYAKEY
HHHHCHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA