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The map label for this gene is ilvB [H]

Identifier: 45358213

GI number: 45358213

Start: 641377

End: 643140

Strand: Direct

Name: ilvB [H]

Synonym: MMP0650

Alternate gene names: 45358213

Gene position: 641377-643140 (Clockwise)

Preceding gene: 45358209

Following gene: 45358214

Centisome position: 38.61

GC content: 39.46

Gene sequence:

>1764_bases
ATGAAAGGAGCAGAGGCTATGATGAAGGCCCTAGAAGCAGAAAATGTAAAAGTCCTTTTTGGATACCCTGGGGGCCAATT
ACTTCCCTTTTACGACGCACTCTATCAAAGTGACCTTTTACACATTTTAACAAGGCACGAACAGGCTGCAGCACACGCGG
CTGATGGATATGCAAGAGCAAGTGGCGATGTAGGAGTTTGTGTTGCTACATCTGGTCCAGGTGCTACAAACCTTGTTACA
GGAGTTGCAACGGCACATGCAGACTCTTCTCCAGTTGTTGCATTAACTGGACAGGTTCCAACAAAATTAATTGGAAATGA
TGCATTCCAAGAAATTGACGCACTTGGAATCTTCATGCCGATTACAAAGCACAACTTCCAGATTCAAAAAACAAGCGAAA
TTCCAAAAATATTTAGAAAAGCATTTGAAATTGCAAAAACGGGAAGGCCCGGTGCAGTTCATGTTGATTTGCCAAAAGAT
GTTCAGGATGATGATTTAGACTTAGAAAAATACCCAATTCCTGCTGAAATTAATCTTCAAGGATATAAACCAACGAAATT
TGGACACCCTTTACAGATCAAAAAAGCTGCAGAATTAATGAAAATCGCACAAAGACCTGTTATTATCGCAGGCGGCGGGG
TTCAGATTGCAAATGCAACTCCTGAACTTATAAAATTATCAGAATATGCTCAAATTCCAGTATGTACTACTTTAATGGGT
AAAGGCGTTTTCCCTGAAGAACACCCTCTTTCATTAGGTATGGTTGGGATGCACGGAACACAAGCTTCAAATTATTCTGT
TTATGAAAGCGATGTTTTAATTGCAATTGGATGTAGGTTTTCAGATAGAATTACCGGTGATCTTTCAAGCTTTGCACCAA
ATACAAAGGTAATCCACATAGATATTGACCCGGCAGAGATTGGAAAGAATGTTGGAGTAGATATCCCAATAGTTGGAGAT
GCAAAAGCAATTTTAAAAGATATTCTGATCCATTTGATGAAAAAAGAAATGGTAAATAAAACAGAATGGATGGAAAATGT
TAAAAAACTTCAGAAAAAGTCCATGCCTGTAATGGAATTTGACAACACGCCGATAAAACCTCAAAAGGTTATTAAAGAAA
TGATGGCAGCACTTAGAGAAGTTGATCCAGGTTTAACAAACACGGTTCTTACTACCGATGTAGGTCAAAATCAGATGTGG
ATGGCACACTATTTCCAAACAAGCGCTCCAAAATCTTTCCTTTCCTCAGGAGGTCAGGGTACAATGGGCTTTGGATTCCC
TGCGGCAATTGGTGCTAAATTTGCAAGGCCTGATGCAAATGTAATTGCTGTAACTGGGGACGGTGGATTTTTAATGAATT
CGCAGGAACTTGCAACAATTGCAGAATACGAAATACCTGTTATCGTTGTGATATTTGACAACCGGACACTTGGAATGGTT
TACCAGTGGCAGAACCTTTATTATGGAAAAAGACAGTGTGCGGTACACCTTGGGGAAACACCTGATTTCTTAAAACTTGC
AGAAAGCTATGGAATCGGTGCTTTGAGAGTTGAAAAACCTGAAGATATTAATGAAGCTTTCAAAACTGCATTAAACAGTG
GAAAACCATATTTACTCGATATCATAATCGACCCGTCAGAAGCACTGCATATGGTACCTCCTGGAGGAAACATGACAAAT
ATATTATTCCCTGACCGACAGGAGCCAACTCCAAAAGCTCAATGCTTTAGCGAAATGAAAAAAATACTTAGTCCTAAGGT
CTAA

Upstream 100 bases:

>100_bases
CAATTTTTATGTTTTCATAATATTTATATAAGATTTTGAGTTAATAAATTAAAAACGAATTAATTTTAAATCCATTACGT
CTTTATTACTTGGTGATCTC

Downstream 100 bases:

>100_bases
TCGGGTGATTGCAATGGAACACAAACACATCATTTCAGTTTTGGTTTTGCACAAACCTGGCGTATTGCAGAGAATTTCAG
GTTTGTTTACCAGAAGATGG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKGAEAMMKALEAENVKVLFGYPGGQLLPFYDALYQSDLLHILTRHEQAAAHAADGYARASGDVGVCVATSGPGATNLVT
GVATAHADSSPVVALTGQVPTKLIGNDAFQEIDALGIFMPITKHNFQIQKTSEIPKIFRKAFEIAKTGRPGAVHVDLPKD
VQDDDLDLEKYPIPAEINLQGYKPTKFGHPLQIKKAAELMKIAQRPVIIAGGGVQIANATPELIKLSEYAQIPVCTTLMG
KGVFPEEHPLSLGMVGMHGTQASNYSVYESDVLIAIGCRFSDRITGDLSSFAPNTKVIHIDIDPAEIGKNVGVDIPIVGD
AKAILKDILIHLMKKEMVNKTEWMENVKKLQKKSMPVMEFDNTPIKPQKVIKEMMAALREVDPGLTNTVLTTDVGQNQMW
MAHYFQTSAPKSFLSSGGQGTMGFGFPAAIGAKFARPDANVIAVTGDGGFLMNSQELATIAEYEIPVIVVIFDNRTLGMV
YQWQNLYYGKRQCAVHLGETPDFLKLAESYGIGALRVEKPEDINEAFKTALNSGKPYLLDIIIDPSEALHMVPPGGNMTN
ILFPDRQEPTPKAQCFSEMKKILSPKV

Sequences:

>Translated_587_residues
MKGAEAMMKALEAENVKVLFGYPGGQLLPFYDALYQSDLLHILTRHEQAAAHAADGYARASGDVGVCVATSGPGATNLVT
GVATAHADSSPVVALTGQVPTKLIGNDAFQEIDALGIFMPITKHNFQIQKTSEIPKIFRKAFEIAKTGRPGAVHVDLPKD
VQDDDLDLEKYPIPAEINLQGYKPTKFGHPLQIKKAAELMKIAQRPVIIAGGGVQIANATPELIKLSEYAQIPVCTTLMG
KGVFPEEHPLSLGMVGMHGTQASNYSVYESDVLIAIGCRFSDRITGDLSSFAPNTKVIHIDIDPAEIGKNVGVDIPIVGD
AKAILKDILIHLMKKEMVNKTEWMENVKKLQKKSMPVMEFDNTPIKPQKVIKEMMAALREVDPGLTNTVLTTDVGQNQMW
MAHYFQTSAPKSFLSSGGQGTMGFGFPAAIGAKFARPDANVIAVTGDGGFLMNSQELATIAEYEIPVIVVIFDNRTLGMV
YQWQNLYYGKRQCAVHLGETPDFLKLAESYGIGALRVEKPEDINEAFKTALNSGKPYLLDIIIDPSEALHMVPPGGNMTN
ILFPDRQEPTPKAQCFSEMKKILSPKV
>Mature_587_residues
MKGAEAMMKALEAENVKVLFGYPGGQLLPFYDALYQSDLLHILTRHEQAAAHAADGYARASGDVGVCVATSGPGATNLVT
GVATAHADSSPVVALTGQVPTKLIGNDAFQEIDALGIFMPITKHNFQIQKTSEIPKIFRKAFEIAKTGRPGAVHVDLPKD
VQDDDLDLEKYPIPAEINLQGYKPTKFGHPLQIKKAAELMKIAQRPVIIAGGGVQIANATPELIKLSEYAQIPVCTTLMG
KGVFPEEHPLSLGMVGMHGTQASNYSVYESDVLIAIGCRFSDRITGDLSSFAPNTKVIHIDIDPAEIGKNVGVDIPIVGD
AKAILKDILIHLMKKEMVNKTEWMENVKKLQKKSMPVMEFDNTPIKPQKVIKEMMAALREVDPGLTNTVLTTDVGQNQMW
MAHYFQTSAPKSFLSSGGQGTMGFGFPAAIGAKFARPDANVIAVTGDGGFLMNSQELATIAEYEIPVIVVIFDNRTLGMV
YQWQNLYYGKRQCAVHLGETPDFLKLAESYGIGALRVEKPEDINEAFKTALNSGKPYLLDIIIDPSEALHMVPPGGNMTN
ILFPDRQEPTPKAQCFSEMKKILSPKV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=26.3620386643234, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=25.6711409395973, Blast_Score=176, Evalue=8e-44,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=42.7574171029668, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=41.2811387900356, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=601, Percent_Identity=33.9434276206323, Blast_Score=347, Evalue=2e-96,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=28.0858676207513, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=25.673249551167, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17531301, Length=494, Percent_Identity=29.3522267206478, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=488, Percent_Identity=29.5081967213115, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=555, Percent_Identity=25.5855855855856, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=41.7699115044248, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320816, Length=561, Percent_Identity=22.2816399286988, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=522, Percent_Identity=25.6704980842912, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=521, Percent_Identity=25.5278310940499, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=528, Percent_Identity=24.8106060606061, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=24.4367417677643, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63805; Mature: 63805

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGAEAMMKALEAENVKVLFGYPGGQLLPFYDALYQSDLLHILTRHEQAAAHAADGYARA
CCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
SGDVGVCVATSGPGATNLVTGVATAHADSSPVVALTGQVPTKLIGNDAFQEIDALGIFMP
CCCEEEEEEECCCCCHHHHHHHEECCCCCCCEEEEECCCCHHHHCCHHHHHHHHHEEEEE
ITKHNFQIQKTSEIPKIFRKAFEIAKTGRPGAVHVDLPKDVQDDDLDLEKYPIPAEINLQ
ECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEC
GYKPTKFGHPLQIKKAAELMKIAQRPVIIAGGGVQIANATPELIKLSEYAQIPVCTTLMG
CCCCCCCCCCEEHHHHHHHHHHHCCCEEEECCCEEEECCCHHHHHHHHHCCCCCHHHHHC
KGVFPEEHPLSLGMVGMHGTQASNYSVYESDVLIAIGCRFSDRITGDLSSFAPNTKVIHI
CCCCCCCCCCEEEEEEECCCCCCCCEEEECCEEEEEECCCCCCCCCCHHHCCCCCEEEEE
DIDPAEIGKNVGVDIPIVGDAKAILKDILIHLMKKEMVNKTEWMENVKKLQKKSMPVMEF
ECCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
DNTPIKPQKVIKEMMAALREVDPGLTNTVLTTDVGQNQMWMAHYFQTSAPKSFLSSGGQG
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHHCCCCHHHHHCCCCC
TMGFGFPAAIGAKFARPDANVIAVTGDGGFLMNSQELATIAEYEIPVIVVIFDNRTLGMV
CCCCCCHHHHCCHHCCCCCCEEEEECCCCEEECCHHHHHHEECCCCEEEEEECCCCEEEE
YQWQNLYYGKRQCAVHLGETPDFLKLAESYGIGALRVEKPEDINEAFKTALNSGKPYLLD
EEECHHCCCCEEEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEE
IIIDPSEALHMVPPGGNMTNILFPDRQEPTPKAQCFSEMKKILSPKV
EEECCCCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGAEAMMKALEAENVKVLFGYPGGQLLPFYDALYQSDLLHILTRHEQAAAHAADGYARA
CCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
SGDVGVCVATSGPGATNLVTGVATAHADSSPVVALTGQVPTKLIGNDAFQEIDALGIFMP
CCCEEEEEEECCCCCHHHHHHHEECCCCCCCEEEEECCCCHHHHCCHHHHHHHHHEEEEE
ITKHNFQIQKTSEIPKIFRKAFEIAKTGRPGAVHVDLPKDVQDDDLDLEKYPIPAEINLQ
ECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEC
GYKPTKFGHPLQIKKAAELMKIAQRPVIIAGGGVQIANATPELIKLSEYAQIPVCTTLMG
CCCCCCCCCCEEHHHHHHHHHHHCCCEEEECCCEEEECCCHHHHHHHHHCCCCCHHHHHC
KGVFPEEHPLSLGMVGMHGTQASNYSVYESDVLIAIGCRFSDRITGDLSSFAPNTKVIHI
CCCCCCCCCCEEEEEEECCCCCCCCEEEECCEEEEEECCCCCCCCCCHHHCCCCCEEEEE
DIDPAEIGKNVGVDIPIVGDAKAILKDILIHLMKKEMVNKTEWMENVKKLQKKSMPVMEF
ECCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
DNTPIKPQKVIKEMMAALREVDPGLTNTVLTTDVGQNQMWMAHYFQTSAPKSFLSSGGQG
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHHCCCCHHHHHCCCCC
TMGFGFPAAIGAKFARPDANVIAVTGDGGFLMNSQELATIAEYEIPVIVVIFDNRTLGMV
CCCCCCHHHHCCHHCCCCCCEEEEECCCCEEECCHHHHHHEECCCCEEEEEECCCCEEEE
YQWQNLYYGKRQCAVHLGETPDFLKLAESYGIGALRVEKPEDINEAFKTALNSGKPYLLD
EEECHHCCCCEEEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEE
IIIDPSEALHMVPPGGNMTNILFPDRQEPTPKAQCFSEMKKILSPKV
EEECCCCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]