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The map label for this gene is tgtA [H]

Identifier: 45358173

GI number: 45358173

Start: 604972

End: 606921

Strand: Direct

Name: tgtA [H]

Synonym: MMP0610

Alternate gene names: 45358173

Gene position: 604972-606921 (Clockwise)

Preceding gene: 45358168

Following gene: 45358174

Centisome position: 36.42

GC content: 34.56

Gene sequence:

>1950_bases
ATGTTTGAAATAAAAGCTCGTGACGCAATGGGGAGACTCGGGAGTATCACGATAAACGGAAAAAAAATTGAAACCCCAAC
AATTATGCCTGTAATTCACCCAAATCCTAAAAAACAGACAGTTTCAATGGATTTAATAAATAAAATGGCAGATGTTGTAA
TTACAAATTCGTATATAACTTACACAACACCGGAACTTAGGGAAATTGCGGAAACAAAAGGAATTCACGAATTAATCGAC
TTTAAAAATGTTGTTGTAACTGACAGCGGTTCATTTCAGTTGAGTGTTTATGGCGATGTAAATGTTGGCCCAATGGAAAT
AATCGATTTTCAGGAAAAAATCGGTGTAGATGTTGGAACGATACTTGATATCCCAACTGGACCTGACGTTTCAAGAGAAA
AAGCTGAAAGCGATCTAATTGAAACATTTAAAAGAGCAGAAGATTCAATTAAAAGAAGAAAAGAAATGGGCTACAAACTC
GCACTTAATGGAACTATTCAAGGTTCAAAATACTTGGATTTAAGGCAGAAAAGTGCTGAAGTTATGGGTAAAATGGACTT
TGATATCTACCCAATTGGTGCAGTAGTTCCTTTAATGGAAGATTACAGATACAGGGAAGTTGCAGAAGTTATTTTAAATT
CAAAAATGCATCTTCCAACAAACAAGCCGGTTCACCTTTTTGGATGCGGACACCCGATGTTATTTGCACTTTCAGTTGCC
CTCGGATGTGATTTATTTGATAGTGCTGCTTATGCTCTTTACGCTAAAAATGGAAGATATTTAACAGCAGATGGAACGCT
TCATCTCAAAGACATGAAAGATTTGAAAAGTTTCCCCTGCACATGTAAAGTATGTAGTGAATACACTCCAAAACAGCTCT
ACAACTTAGAAGAAAAGGAAAAAACGAGATTACTTGCAGAACACAATTTATACGTTACTTTTGAAGAAATTGATAGAATT
AAAAATGCAATAAAAGAAGGAAACCTTTGGGAACTCGTTGAAGAAAGGTGTAGAAGCCACCCTAAACTTTTGAACGGTTT
GAGAGTTATTTCAAAATATATGGATTTCATTGAAAAACACGATCCGGTTTCTAAAAAATCAGGATTTTTCTACACAGGAT
ATGAAAGCATGAATAGGCCTGAAATTTACAGGCACAAACAAAGGCTTGATAGGATTCAATACGATAAAATATACGTAACA
AGTGTGTCTGAAAATACATCAAAGCCGTACAGTGAAAATTTAAGCAATGTTCCTTGCGATGTTGATGTACTTGTAAAAGA
CAGTGTATTTGGATTAGTTCCACTAAATATCGATACAATGTATCCTCTTGCTCAAAATGAAGTTCCAGATTTGTATGATT
TTGAAAAAAAATACAACAATGAATTTGTAAGCGAATTTAATGAAAAACACGCTGAAAAAATACTCGATATAAGCACATAC
AATTACTACATAAATCACTACGGCAAGAAAAAAGAGTGCGACAAAATCAATCCTGATATTTTCAGAATTGGAAAAATGCT
CGAATACCAGTATGGGGCTAAAATACTCGATGAAGAATTAATGGAAAAAGTAAAATCAAGAAGAAGTAAAAATACCGGCA
GAATTAGAAATCTTTTGCTCGAAAAGGAAGTTTTATTTACATTACGGGCAAATGATAACTTTTTAATTCCTGCAAAATCC
GGTGCTGAACTTTTACATGAAAAACTCGAGTTTCCAAAGTACAGGATAGTAATCGACAGCAGTGTTGAAGAATTTGCAAG
GGCTGGAAAATCCGTTTATTCCAAATTCGTTAAAGACTGTGATCCAGAATTAAGGCCATTTGAAGAAGTTTTAATTGTAA
ATTCTGATGATGAATTATTGGCATACGGTACAACAATACTAAATGGTCGTGAATTGATGGAATTTGATTACGGCGTTGCA
GCAACCCTTCGAGGGGGATTGAAGAAATAA

Upstream 100 bases:

>100_bases
AAGTAATATATCTAATAGTTAATTGCTATTTTAACATTTGGGATTATTATTGAAAATTATCGATTTTTACAGATTATGAA
GTTTAATTTATGGTGGAAAC

Downstream 100 bases:

>100_bases
GTTTATTAACTTAAACTTTAAATAAAATATTGTTTATTCTGGACGTGATTTTTTGTTAAAAGTATTGTATAGTGGTTCGG
TTACGTATCTTGATGAGAAA

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MFEIKARDAMGRLGSITINGKKIETPTIMPVIHPNPKKQTVSMDLINKMADVVITNSYITYTTPELREIAETKGIHELID
FKNVVVTDSGSFQLSVYGDVNVGPMEIIDFQEKIGVDVGTILDIPTGPDVSREKAESDLIETFKRAEDSIKRRKEMGYKL
ALNGTIQGSKYLDLRQKSAEVMGKMDFDIYPIGAVVPLMEDYRYREVAEVILNSKMHLPTNKPVHLFGCGHPMLFALSVA
LGCDLFDSAAYALYAKNGRYLTADGTLHLKDMKDLKSFPCTCKVCSEYTPKQLYNLEEKEKTRLLAEHNLYVTFEEIDRI
KNAIKEGNLWELVEERCRSHPKLLNGLRVISKYMDFIEKHDPVSKKSGFFYTGYESMNRPEIYRHKQRLDRIQYDKIYVT
SVSENTSKPYSENLSNVPCDVDVLVKDSVFGLVPLNIDTMYPLAQNEVPDLYDFEKKYNNEFVSEFNEKHAEKILDISTY
NYYINHYGKKKECDKINPDIFRIGKMLEYQYGAKILDEELMEKVKSRRSKNTGRIRNLLLEKEVLFTLRANDNFLIPAKS
GAELLHEKLEFPKYRIVIDSSVEEFARAGKSVYSKFVKDCDPELRPFEEVLIVNSDDELLAYGTTILNGRELMEFDYGVA
ATLRGGLKK

Sequences:

>Translated_649_residues
MFEIKARDAMGRLGSITINGKKIETPTIMPVIHPNPKKQTVSMDLINKMADVVITNSYITYTTPELREIAETKGIHELID
FKNVVVTDSGSFQLSVYGDVNVGPMEIIDFQEKIGVDVGTILDIPTGPDVSREKAESDLIETFKRAEDSIKRRKEMGYKL
ALNGTIQGSKYLDLRQKSAEVMGKMDFDIYPIGAVVPLMEDYRYREVAEVILNSKMHLPTNKPVHLFGCGHPMLFALSVA
LGCDLFDSAAYALYAKNGRYLTADGTLHLKDMKDLKSFPCTCKVCSEYTPKQLYNLEEKEKTRLLAEHNLYVTFEEIDRI
KNAIKEGNLWELVEERCRSHPKLLNGLRVISKYMDFIEKHDPVSKKSGFFYTGYESMNRPEIYRHKQRLDRIQYDKIYVT
SVSENTSKPYSENLSNVPCDVDVLVKDSVFGLVPLNIDTMYPLAQNEVPDLYDFEKKYNNEFVSEFNEKHAEKILDISTY
NYYINHYGKKKECDKINPDIFRIGKMLEYQYGAKILDEELMEKVKSRRSKNTGRIRNLLLEKEVLFTLRANDNFLIPAKS
GAELLHEKLEFPKYRIVIDSSVEEFARAGKSVYSKFVKDCDPELRPFEEVLIVNSDDELLAYGTTILNGRELMEFDYGVA
ATLRGGLKK
>Mature_649_residues
MFEIKARDAMGRLGSITINGKKIETPTIMPVIHPNPKKQTVSMDLINKMADVVITNSYITYTTPELREIAETKGIHELID
FKNVVVTDSGSFQLSVYGDVNVGPMEIIDFQEKIGVDVGTILDIPTGPDVSREKAESDLIETFKRAEDSIKRRKEMGYKL
ALNGTIQGSKYLDLRQKSAEVMGKMDFDIYPIGAVVPLMEDYRYREVAEVILNSKMHLPTNKPVHLFGCGHPMLFALSVA
LGCDLFDSAAYALYAKNGRYLTADGTLHLKDMKDLKSFPCTCKVCSEYTPKQLYNLEEKEKTRLLAEHNLYVTFEEIDRI
KNAIKEGNLWELVEERCRSHPKLLNGLRVISKYMDFIEKHDPVSKKSGFFYTGYESMNRPEIYRHKQRLDRIQYDKIYVT
SVSENTSKPYSENLSNVPCDVDVLVKDSVFGLVPLNIDTMYPLAQNEVPDLYDFEKKYNNEFVSEFNEKHAEKILDISTY
NYYINHYGKKKECDKINPDIFRIGKMLEYQYGAKILDEELMEKVKSRRSKNTGRIRNLLLEKEVLFTLRANDNFLIPAKS
GAELLHEKLEFPKYRIVIDSSVEEFARAGKSVYSKFVKDCDPELRPFEEVLIVNSDDELLAYGTTILNGRELMEFDYGVA
ATLRGGLKK

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=342, Percent_Identity=24.2690058479532, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=26.3013698630137, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17544680, Length=366, Percent_Identity=22.6775956284153, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=23.8227146814404, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616
- InterPro:   IPR004521 [H]

Pfam domain/function: PF01472 PUA; PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 74402; Mature: 74402

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIKARDAMGRLGSITINGKKIETPTIMPVIHPNPKKQTVSMDLINKMADVVITNSYIT
CCEECCHHHCCCCCEEEECCEEECCCEEEEEECCCCCCCEEHHHHHHHHHHHEEECCEEE
YTTPELREIAETKGIHELIDFKNVVVTDSGSFQLSVYGDVNVGPMEIIDFQEKIGVDVGT
ECCHHHHHHHHHCCHHHHHCCCCEEEECCCCEEEEEEECCCCCCHHHCCHHHHHCCCCCE
ILDIPTGPDVSREKAESDLIETFKRAEDSIKRRKEMGYKLALNGTIQGSKYLDLRQKSAE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEECCCHHHHHHHHHHH
VMGKMDFDIYPIGAVVPLMEDYRYREVAEVILNSKMHLPTNKPVHLFGCGHPMLFALSVA
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHH
LGCDLFDSAAYALYAKNGRYLTADGTLHLKDMKDLKSFPCTCKVCSEYTPKQLYNLEEKE
HCCCHHHCCEEEEEECCCEEEEECCEEEECCHHHHHCCCCEEHHHCCCCHHHHHCCCHHH
KTRLLAEHNLYVTFEEIDRIKNAIKEGNLWELVEERCRSHPKLLNGLRVISKYMDFIEKH
HHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
DPVSKKSGFFYTGYESMNRPEIYRHKQRLDRIQYDKIYVTSVSENTSKPYSENLSNVPCD
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCC
VDVLVKDSVFGLVPLNIDTMYPLAQNEVPDLYDFEKKYNNEFVSEFNEKHAEKILDISTY
EEEEEECCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHE
NYYINHYGKKKECDKINPDIFRIGKMLEYQYGAKILDEELMEKVKSRRSKNTGRIRNLLL
EEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EKEVLFTLRANDNFLIPAKSGAELLHEKLEFPKYRIVIDSSVEEFARAGKSVYSKFVKDC
HHEEEEEEECCCCEEEECCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
DPELRPFEEVLIVNSDDELLAYGTTILNGRELMEFDYGVAATLRGGLKK
CCCCCCCEEEEEECCCCCEEEECEEEECCCHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MFEIKARDAMGRLGSITINGKKIETPTIMPVIHPNPKKQTVSMDLINKMADVVITNSYIT
CCEECCHHHCCCCCEEEECCEEECCCEEEEEECCCCCCCEEHHHHHHHHHHHEEECCEEE
YTTPELREIAETKGIHELIDFKNVVVTDSGSFQLSVYGDVNVGPMEIIDFQEKIGVDVGT
ECCHHHHHHHHHCCHHHHHCCCCEEEECCCCEEEEEEECCCCCCHHHCCHHHHHCCCCCE
ILDIPTGPDVSREKAESDLIETFKRAEDSIKRRKEMGYKLALNGTIQGSKYLDLRQKSAE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEECCCHHHHHHHHHHH
VMGKMDFDIYPIGAVVPLMEDYRYREVAEVILNSKMHLPTNKPVHLFGCGHPMLFALSVA
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHH
LGCDLFDSAAYALYAKNGRYLTADGTLHLKDMKDLKSFPCTCKVCSEYTPKQLYNLEEKE
HCCCHHHCCEEEEEECCCEEEEECCEEEECCHHHHHCCCCEEHHHCCCCHHHHHCCCHHH
KTRLLAEHNLYVTFEEIDRIKNAIKEGNLWELVEERCRSHPKLLNGLRVISKYMDFIEKH
HHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
DPVSKKSGFFYTGYESMNRPEIYRHKQRLDRIQYDKIYVTSVSENTSKPYSENLSNVPCD
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCC
VDVLVKDSVFGLVPLNIDTMYPLAQNEVPDLYDFEKKYNNEFVSEFNEKHAEKILDISTY
EEEEEECCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHE
NYYINHYGKKKECDKINPDIFRIGKMLEYQYGAKILDEELMEKVKSRRSKNTGRIRNLLL
EEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EKEVLFTLRANDNFLIPAKSGAELLHEKLEFPKYRIVIDSSVEEFARAGKSVYSKFVKDC
HHEEEEEEECCCCEEEECCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
DPELRPFEEVLIVNSDDELLAYGTTILNGRELMEFDYGVAATLRGGLKK
CCCCCCCEEEEEECCCCCEEEECEEEECCCHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA