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The map label for this gene is gltB [C]

Identifier: 45358059

GI number: 45358059

Start: 499642

End: 501126

Strand: Direct

Name: gltB [C]

Synonym: MMP0496

Alternate gene names: 45358059

Gene position: 499642-501126 (Clockwise)

Preceding gene: 45358050

Following gene: 45358061

Centisome position: 30.08

GC content: 34.88

Gene sequence:

>1485_bases
ATGGCCAAATATCGATGTAACGTTTGCAATGTCTTTGAATATGATGAATCAAGAGGGGATTCAGTTACAAATATAAAACC
CGGTACAAAAATAAAAGATTTTCCAAATAACTGGAAATGCCCAATCTGCGGATCTGACTCTACTCATTTAATCCAGATAG
CTGAAACAAATATTTCTAAAAAACTTAAAGGGACTTTTGAATGTCCTGAATGCAAATCAAAGTCAGAAATTGAAATTTCA
ATGCCTATCAAAATGGATTCAAGTTATCTGGGGGAATGGAAAAGAGAATACGATGATATGGAAGAACACATGTTTGACAT
TCATAAAATATCGGTAACTGGGGAATCAATAATTGAACCAATGAGGACAAAAAAACCAGTAATATCCTGGGAAAGTATAT
TTATTAAAGGTGCACAGCTTGCAAAATTCCCTTTAAACAACGATGAAGAAGTTATTACAAAAACAATCATTGGACCTAAA
GCAAAACATCCTTTAATCATTGAAACTCCAATTTTTATCACCCACATGTCTTATGGGGCACTTTCAAAAAATGTAAAAAT
TGCTCTTTCAAAGGGTAGTGCAGCTGTGAAAACTGCAATGTGTTCTGGGGAGGGTGGAATGCTTGAAGAATCCTTCGAAA
ATGCATATAAATACATTTTAGAGTATGTACCAAACCAGTACAGCATAACAGATGAAAATTTAAAAAAAGTAGATGCAGTC
GAAATAAAAATTGGCCAGTCTTCAAAACCCGGAATGGGGGGACACCTTCCCGCAGAAAAAGTTTCAGAAGAAATCGCTAA
ATTAAGGGGTTTTAAAGTAGGGCAGGATATTGTAAGCCCGTCTAAATTTCATGATATTAATAATAAGGATGATTTAAAAA
ATAAAGTAAGTAATTTAAGAGAAAAATCCGGCGGAAAACCAATTGGAATTAAAATCGCTGCAGGAGATATTGAAGCGGAT
TTAGAAGTTGCAACATATGCAAAACCAGATTTTATTACAATTGATGGAAGACCAGGGGCAACAGCCGCATCTCCAAAATT
TATTAAAGATTCAACATCTGTTCCAACAATTTTTGCACTTTATCGAGCAAGAGAATTTTTTAATAAAAATGGAATAACAG
ATATATCTTTGGTTATAACTGGGGGCCTTAGAATATCTTCTGATTTTGCAAAGGCAATTGCAATTGGTGCAGATGCAATA
GCTATCGGTACTGCAGCGTTAATGGCAACTGCATGTCAGCAGTATCGGATTTGTGACACAGGGGAGTGTCCCGTTGGAGT
TACAACACAAAAACCAGAGTTAAAAGATCGGTTAAAAATTGAATTGTCCGCAAAAAAACTATCGAATTATTTGCGAGTGT
CGACCGAAGAATTAAAAACATTTGCAAGGCTTACGGGGAATAAAAATATTCATGATCTCTCTGTTGATGATTTGTGTACG
AGTGATTCGGAAATTTCTAATTTTACCTCCATAAAACATGTTTAA

Upstream 100 bases:

>100_bases
GACCATATTTTTAGATGCGTATAGATATGATTTATTAAACGCCAATTACATAATTTAATCAGGACTTTTAATTAATAATT
TAATCATTTGGGGGAGTTCA

Downstream 100 bases:

>100_bases
ATATTTTTTCAATATTAAAAAAGCATGAATAATTGTTAGTTTTCTAAAATGGCGGAAAAATAAAGTAATGATCCGTTTTT
AAAAGGATATGGCCTAAATG

Product: glutamate synthase subunit-related

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAKYRCNVCNVFEYDESRGDSVTNIKPGTKIKDFPNNWKCPICGSDSTHLIQIAETNISKKLKGTFECPECKSKSEIEIS
MPIKMDSSYLGEWKREYDDMEEHMFDIHKISVTGESIIEPMRTKKPVISWESIFIKGAQLAKFPLNNDEEVITKTIIGPK
AKHPLIIETPIFITHMSYGALSKNVKIALSKGSAAVKTAMCSGEGGMLEESFENAYKYILEYVPNQYSITDENLKKVDAV
EIKIGQSSKPGMGGHLPAEKVSEEIAKLRGFKVGQDIVSPSKFHDINNKDDLKNKVSNLREKSGGKPIGIKIAAGDIEAD
LEVATYAKPDFITIDGRPGATAASPKFIKDSTSVPTIFALYRAREFFNKNGITDISLVITGGLRISSDFAKAIAIGADAI
AIGTAALMATACQQYRICDTGECPVGVTTQKPELKDRLKIELSAKKLSNYLRVSTEELKTFARLTGNKNIHDLSVDDLCT
SDSEISNFTSIKHV

Sequences:

>Translated_494_residues
MAKYRCNVCNVFEYDESRGDSVTNIKPGTKIKDFPNNWKCPICGSDSTHLIQIAETNISKKLKGTFECPECKSKSEIEIS
MPIKMDSSYLGEWKREYDDMEEHMFDIHKISVTGESIIEPMRTKKPVISWESIFIKGAQLAKFPLNNDEEVITKTIIGPK
AKHPLIIETPIFITHMSYGALSKNVKIALSKGSAAVKTAMCSGEGGMLEESFENAYKYILEYVPNQYSITDENLKKVDAV
EIKIGQSSKPGMGGHLPAEKVSEEIAKLRGFKVGQDIVSPSKFHDINNKDDLKNKVSNLREKSGGKPIGIKIAAGDIEAD
LEVATYAKPDFITIDGRPGATAASPKFIKDSTSVPTIFALYRAREFFNKNGITDISLVITGGLRISSDFAKAIAIGADAI
AIGTAALMATACQQYRICDTGECPVGVTTQKPELKDRLKIELSAKKLSNYLRVSTEELKTFARLTGNKNIHDLSVDDLCT
SDSEISNFTSIKHV
>Mature_493_residues
AKYRCNVCNVFEYDESRGDSVTNIKPGTKIKDFPNNWKCPICGSDSTHLIQIAETNISKKLKGTFECPECKSKSEIEISM
PIKMDSSYLGEWKREYDDMEEHMFDIHKISVTGESIIEPMRTKKPVISWESIFIKGAQLAKFPLNNDEEVITKTIIGPKA
KHPLIIETPIFITHMSYGALSKNVKIALSKGSAAVKTAMCSGEGGMLEESFENAYKYILEYVPNQYSITDENLKKVDAVE
IKIGQSSKPGMGGHLPAEKVSEEIAKLRGFKVGQDIVSPSKFHDINNKDDLKNKVSNLREKSGGKPIGIKIAAGDIEADL
EVATYAKPDFITIDGRPGATAASPKFIKDSTSVPTIFALYRAREFFNKNGITDISLVITGGLRISSDFAKAIAIGADAIA
IGTAALMATACQQYRICDTGECPVGVTTQKPELKDRLKIELSAKKLSNYLRVSTEELKTFARLTGNKNIHDLSVDDLCTS
DSEISNFTSIKHV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=304, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=312, Percent_Identity=28.2051282051282, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320030, Length=309, Percent_Identity=27.5080906148867, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28574881, Length=306, Percent_Identity=27.4509803921569, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24665539, Length=306, Percent_Identity=27.4509803921569, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54376; Mature: 54245

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYRCNVCNVFEYDESRGDSVTNIKPGTKIKDFPNNWKCPICGSDSTHLIQIAETNISK
CCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCH
KLKGTFECPECKSKSEIEISMPIKMDSSYLGEWKREYDDMEEHMFDIHKISVTGESIIEP
HHCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHH
MRTKKPVISWESIFIKGAQLAKFPLNNDEEVITKTIIGPKAKHPLIIETPIFITHMSYGA
HHHCCCCEEEHHEEEECCEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCEEEEEECCCC
LSKNVKIALSKGSAAVKTAMCSGEGGMLEESFENAYKYILEYVPNQYSITDENLKKVDAV
CCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHCCEEEE
EIKIGQSSKPGMGGHLPAEKVSEEIAKLRGFKVGQDIVSPSKFHDINNKDDLKNKVSNLR
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHCCCCCHHHHHHHHHHHH
EKSGGKPIGIKIAAGDIEADLEVATYAKPDFITIDGRPGATAASPKFIKDSTSVPTIFAL
HHCCCCEEEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
YRAREFFNKNGITDISLVITGGLRISSDFAKAIAIGADAIAIGTAALMATACQQYRICDT
HHHHHHHCCCCCCEEEEEEECCEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
GECPVGVTTQKPELKDRLKIELSAKKLSNYLRVSTEELKTFARLTGNKNIHDLSVDDLCT
CCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHCCCCCEEECCHHHHCC
SDSEISNFTSIKHV
CCCHHHHHHHCCCC
>Mature Secondary Structure 
AKYRCNVCNVFEYDESRGDSVTNIKPGTKIKDFPNNWKCPICGSDSTHLIQIAETNISK
CCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCH
KLKGTFECPECKSKSEIEISMPIKMDSSYLGEWKREYDDMEEHMFDIHKISVTGESIIEP
HHCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHH
MRTKKPVISWESIFIKGAQLAKFPLNNDEEVITKTIIGPKAKHPLIIETPIFITHMSYGA
HHHCCCCEEEHHEEEECCEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCEEEEEECCCC
LSKNVKIALSKGSAAVKTAMCSGEGGMLEESFENAYKYILEYVPNQYSITDENLKKVDAV
CCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHCCEEEE
EIKIGQSSKPGMGGHLPAEKVSEEIAKLRGFKVGQDIVSPSKFHDINNKDDLKNKVSNLR
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHCCCCCHHHHHHHHHHHH
EKSGGKPIGIKIAAGDIEADLEVATYAKPDFITIDGRPGATAASPKFIKDSTSVPTIFAL
HHCCCCEEEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
YRAREFFNKNGITDISLVITGGLRISSDFAKAIAIGADAIAIGTAALMATACQQYRICDT
HHHHHHHCCCCCCEEEEEEECCEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
GECPVGVTTQKPELKDRLKIELSAKKLSNYLRVSTEELKTFARLTGNKNIHDLSVDDLCT
CCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHCCCCCEEECCHHHHCC
SDSEISNFTSIKHV
CCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]