Click here to switch to the map view.

The map label for this gene is nfsA [C]

Identifier: 45357989

GI number: 45357989

Start: 427592

End: 428161

Strand: Direct

Name: nfsA [C]

Synonym: MMP0426

Alternate gene names: 45357989

Gene position: 427592-428161 (Clockwise)

Preceding gene: 45357988

Following gene: 45357992

Centisome position: 25.74

GC content: 35.26

Gene sequence:

>570_bases
ATGAATCAGGTTTTAGAAACAATAAAAAACAGGAGAAGTGTCAGAAAGTTTAAAAAAGAGCAACTAAAAGACAGTGAAAT
TGAAGCAATACTCGAAGCTGCAATTTATGCGCCAACTGCACTAAATGAACAGCCCTGGCACTTTACAGTAATTCAGAACA
AGGAATTGCTCGACGAAATAAATACCGTATCTAAAAAGACTCTCGCAGAAAATCCAGAATCAATGAAAAATATGCCTGAA
AGTCTTATAAATGCAATGAGCAATCCAAAATACAACATCCTTTACGAAGCTCCAACTTTAATAGTCGTTTCAGGAAATAA
AAACGCACATGCAGCGATTGTTGATTGTTCTGCGGCAATTCAGAACATGCTTTTGGCAGCAGAAAGTATGAATATCGGTT
CAGTATGGCTTGGACTTGTAAGGCCGTACTTTACAACCGAAAATGTTAAAAAATTAAATATTCCAGAAGAATTTGAATCA
TTCTACGGGGTTGCATTTGGTTACAAATTGATAGAAAAATCAAAGACAGCGCCTGAGCGAAAAAAAGACGTTGTAAATTA
CATAAAATAA

Upstream 100 bases:

>100_bases
GCTTGCAGAATTTATTCAGTAATTAAAAGAAGACCAATGCTTACAGATATGACTGTGCTTGTTGTAGGAAAGAGTCTTGG
ATTCTAAATTTGGTGAAAGC

Downstream 100 bases:

>100_bases
TTTCTCTTTTGATTTTTTGAAAATTTAATTTAGAAAAATATTTTAAAAAATTTAAAAAAAGTTTATTCTAAAATTCCCAT
TTTTGCAAGGAGTGCACTTA

Product: nitroreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MNQVLETIKNRRSVRKFKKEQLKDSEIEAILEAAIYAPTALNEQPWHFTVIQNKELLDEINTVSKKTLAENPESMKNMPE
SLINAMSNPKYNILYEAPTLIVVSGNKNAHAAIVDCSAAIQNMLLAAESMNIGSVWLGLVRPYFTTENVKKLNIPEEFES
FYGVAFGYKLIEKSKTAPERKKDVVNYIK

Sequences:

>Translated_189_residues
MNQVLETIKNRRSVRKFKKEQLKDSEIEAILEAAIYAPTALNEQPWHFTVIQNKELLDEINTVSKKTLAENPESMKNMPE
SLINAMSNPKYNILYEAPTLIVVSGNKNAHAAIVDCSAAIQNMLLAAESMNIGSVWLGLVRPYFTTENVKKLNIPEEFES
FYGVAFGYKLIEKSKTAPERKKDVVNYIK
>Mature_189_residues
MNQVLETIKNRRSVRKFKKEQLKDSEIEAILEAAIYAPTALNEQPWHFTVIQNKELLDEINTVSKKTLAENPESMKNMPE
SLINAMSNPKYNILYEAPTLIVVSGNKNAHAAIVDCSAAIQNMLLAAESMNIGSVWLGLVRPYFTTENVKKLNIPEEFES
FYGVAFGYKLIEKSKTAPERKKDVVNYIK

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 21410; Mature: 21410

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVLETIKNRRSVRKFKKEQLKDSEIEAILEAAIYAPTALNEQPWHFTVIQNKELLDEI
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHH
NTVSKKTLAENPESMKNMPESLINAMSNPKYNILYEAPTLIVVSGNKNAHAAIVDCSAAI
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCEEEEEHHHHH
QNMLLAAESMNIGSVWLGLVRPYFTTENVKKLNIPEEFESFYGVAFGYKLIEKSKTAPER
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
KKDVVNYIK
HHHHHHHCC
>Mature Secondary Structure
MNQVLETIKNRRSVRKFKKEQLKDSEIEAILEAAIYAPTALNEQPWHFTVIQNKELLDEI
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHH
NTVSKKTLAENPESMKNMPESLINAMSNPKYNILYEAPTLIVVSGNKNAHAAIVDCSAAI
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCEEEEEHHHHH
QNMLLAAESMNIGSVWLGLVRPYFTTENVKKLNIPEEFESFYGVAFGYKLIEKSKTAPER
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
KKDVVNYIK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]