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The map label for this gene is opuE [H]

Identifier: 45357783

GI number: 45357783

Start: 227364

End: 228833

Strand: Direct

Name: opuE [H]

Synonym: MMP0220

Alternate gene names: 45357783

Gene position: 227364-228833 (Clockwise)

Preceding gene: 45357782

Following gene: 45357784

Centisome position: 13.69

GC content: 37.82

Gene sequence:

>1470_bases
ATGTGGCACTACGTGTTTTTGGCGGTTTATGTTGCTATGATTATAGGAATAGCTACTGTAACGAAAAAAAGAATAAAAGG
GGTTTCTGACTTTTTACTAGGGGGAAGAAACGTAAACCCATGGATGTCTGCGTTTTCGTACGGTACTGCTTATTTTTCAG
CGGTTATTTTTATCGGATTTGCTGGAAAAATGGGTTGGAGTTTTGGAATCCCGACAATGTGGATAGTTGTTGGAAATACG
ATTATTGGAAGTTTTTTGGCATGGCAGGTTCTAGGTAAAAAAACAAGAGAAATGACGCAACGATTGGGTGCATCAACAAT
GCCTAGTTTTTTAGAAAAAAGGTACAAAAGCAAGAATTTAGAATCACTAACTGCGTTTATCATCTTTTTCTTCTTAGTTC
CTTATTCTGCATCTATTTATAAAGGTTTAAATTTCTTATTTGAAGGATTTGGAATATCTCCAATGAATGCGTATATTTTA
ATGGCCGCATTAACTGCAATATACTTGTATTTTGGAGGATTTATTGCGGCAACACTTGCAGACTTCGTTCAAGGGTGCAT
CATGGTAATTGGGGTACTTTTAATGGTATTCTTTTTAGGCAGCAACCCCGAAGTTGGCGGGTTTTCTGGAATGCTGTCGA
ATTTAAGTTCGATAGATCCAATGCTGGTAACCCCGATAAATGAAGCAAGCTTTATTCCAATAATGGGTCTTGCATTGCTC
ACGAGTATTGGTAGCTGGGGCCTTCCGCAGATGATACACAAATTCTACACGATAAAAAGTGAAAAAGCAGCAGTTTCTGC
AAAATGGGTTTCAACAGCATTTGCATTCATCATTACTTTTGGTGCAGCATATATTGGAACCGTAAGTAGACTTTTCTATC
CCGACGCTGCTTCAAAAGCTGCAGCCGGCTTAACGAGCCCTGATATGATAGTTCCAAAAATAATGGAAATTGCACTTCCT
GACTGGGTTGCGGCATTAATTCTCGTACTCGTAATTTCAGCTTCAATGTCTACTTTAGCATCACTAGTGCTTGCTTCAAG
TTCCGTTGTTTCAATAGACTTTGTAAAAGGAAGATTAAAGACTGATTTATCGGATAAAAACACGATGATATTGATGAGAT
TATTGTGCGTTTTATTTGTTGTATTATCGTTCATACTTGCAATAGTTCCAAACAGCTATGTTCTATCATTAATGGCCCTT
TCATGGGGTTTGGTATCAGGATGTCTTTTGGCTCCGTACTTTTTAGGATTATACTGGAAAAGAACTACGAAAGAAGGAGT
ATGGGCAGGAATTACATCTGCTCTCGCAATAATGCTTGGGGGAGCATATTACTTTGGAATAAACAGCGTTTATATGCCCG
CAGTTGGTTCTTTAGCAATGATTGCACCGATATTTGTTGTAATGGCGGTAAGTCTTATCACAAAACCATTTGAACGTGAA
CACATTGACTACATATTCAATAAAAAATAA

Upstream 100 bases:

>100_bases
AAAAATTAGATAAATATAAATAAAAATGCCATAAAATAGGAATTATATTGTTGAATTTTAACTATTTTTTCAAATGTTAT
CAAAGTTTTTGAGGTTAATT

Downstream 100 bases:

>100_bases
CTTTTTTTAATAATTTTTATTCCATTTCGTAATTTTTAGAGACTTTTGAATGATTTTTCCCAAATTTTTTAAGTAATTTT
AACTGCCAACTCAAAAACGT

Product: Sodium/proline symporter-related

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MWHYVFLAVYVAMIIGIATVTKKRIKGVSDFLLGGRNVNPWMSAFSYGTAYFSAVIFIGFAGKMGWSFGIPTMWIVVGNT
IIGSFLAWQVLGKKTREMTQRLGASTMPSFLEKRYKSKNLESLTAFIIFFFLVPYSASIYKGLNFLFEGFGISPMNAYIL
MAALTAIYLYFGGFIAATLADFVQGCIMVIGVLLMVFFLGSNPEVGGFSGMLSNLSSIDPMLVTPINEASFIPIMGLALL
TSIGSWGLPQMIHKFYTIKSEKAAVSAKWVSTAFAFIITFGAAYIGTVSRLFYPDAASKAAAGLTSPDMIVPKIMEIALP
DWVAALILVLVISASMSTLASLVLASSSVVSIDFVKGRLKTDLSDKNTMILMRLLCVLFVVLSFILAIVPNSYVLSLMAL
SWGLVSGCLLAPYFLGLYWKRTTKEGVWAGITSALAIMLGGAYYFGINSVYMPAVGSLAMIAPIFVVMAVSLITKPFERE
HIDYIFNKK

Sequences:

>Translated_489_residues
MWHYVFLAVYVAMIIGIATVTKKRIKGVSDFLLGGRNVNPWMSAFSYGTAYFSAVIFIGFAGKMGWSFGIPTMWIVVGNT
IIGSFLAWQVLGKKTREMTQRLGASTMPSFLEKRYKSKNLESLTAFIIFFFLVPYSASIYKGLNFLFEGFGISPMNAYIL
MAALTAIYLYFGGFIAATLADFVQGCIMVIGVLLMVFFLGSNPEVGGFSGMLSNLSSIDPMLVTPINEASFIPIMGLALL
TSIGSWGLPQMIHKFYTIKSEKAAVSAKWVSTAFAFIITFGAAYIGTVSRLFYPDAASKAAAGLTSPDMIVPKIMEIALP
DWVAALILVLVISASMSTLASLVLASSSVVSIDFVKGRLKTDLSDKNTMILMRLLCVLFVVLSFILAIVPNSYVLSLMAL
SWGLVSGCLLAPYFLGLYWKRTTKEGVWAGITSALAIMLGGAYYFGINSVYMPAVGSLAMIAPIFVVMAVSLITKPFERE
HIDYIFNKK
>Mature_489_residues
MWHYVFLAVYVAMIIGIATVTKKRIKGVSDFLLGGRNVNPWMSAFSYGTAYFSAVIFIGFAGKMGWSFGIPTMWIVVGNT
IIGSFLAWQVLGKKTREMTQRLGASTMPSFLEKRYKSKNLESLTAFIIFFFLVPYSASIYKGLNFLFEGFGISPMNAYIL
MAALTAIYLYFGGFIAATLADFVQGCIMVIGVLLMVFFLGSNPEVGGFSGMLSNLSSIDPMLVTPINEASFIPIMGLALL
TSIGSWGLPQMIHKFYTIKSEKAAVSAKWVSTAFAFIITFGAAYIGTVSRLFYPDAASKAAAGLTSPDMIVPKIMEIALP
DWVAALILVLVISASMSTLASLVLASSSVVSIDFVKGRLKTDLSDKNTMILMRLLCVLFVVLSFILAIVPNSYVLSLMAL
SWGLVSGCLLAPYFLGLYWKRTTKEGVWAGITSALAIMLGGAYYFGINSVYMPAVGSLAMIAPIFVVMAVSLITKPFERE
HIDYIFNKK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=440, Percent_Identity=24.3181818181818, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI14140236, Length=531, Percent_Identity=20.9039548022599, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI206597483, Length=382, Percent_Identity=24.3455497382199, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI206597487, Length=382, Percent_Identity=24.3455497382199, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157671931, Length=483, Percent_Identity=23.6024844720497, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI110835708, Length=545, Percent_Identity=23.302752293578, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI17941285, Length=452, Percent_Identity=23.6725663716814, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4507031, Length=369, Percent_Identity=21.9512195121951, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787251, Length=445, Percent_Identity=26.9662921348315, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87082237, Length=460, Percent_Identity=28.2608695652174, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790503, Length=458, Percent_Identity=22.2707423580786, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53285; Mature: 53285

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCC
>Mature Secondary Structure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HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]