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The map label for this gene is argG

Identifier: 45357636

GI number: 45357636

Start: 84962

End: 86155

Strand: Direct

Name: argG

Synonym: MMP0073

Alternate gene names: 45357636

Gene position: 84962-86155 (Clockwise)

Preceding gene: 45357635

Following gene: 45357637

Centisome position: 5.11

GC content: 37.02

Gene sequence:

>1194_bases
ATGCAAGAAAAAATTGCAGTTTTGGCATATTCTGGAGGGCTTGACACGAGCTGCTGTTTAAAATTACTCGAAGACAAATA
CGACTACAAGGTAATTTCAGTAGCTGTAGATGTAGGCCAGCCTGAAGACGATTTAAAAGAACCAGAAGAAAAAGCAAAAA
AATTCGGTGTATTAAAACACTATACTATCGATGCAAAGGAAGAATTCGCAAAAGATTACATATTTAGGGCTATTAAAGCA
AATGCGCTTTACGAAGGATACCCATTATCAACCGCTCTTGCAAGACCTTTAATTGCTATAAAAATTGCAGAACTTGCACA
AGAAGTTGGAGCAAGCGCAATTTCACACGGATGTACTGGAAAAGGAAACGACCAGTTCAGATTTGAATCTGTAATGAGAG
CAAAAACACCTGAAATTGAAATTGTAGCGCCAATCAGAGATTTAAACCTTACAAGAACTGAAGAAATAGCATATGCAAAA
GAAAAAGGAATTCCAGTTCCAGTAGACTTAGAAAAACCATTCAGTATCGATGAAAATTTATGGGGAAGAAGTATTGAGGG
CGGAATTTTAGAAGACCCAATGACTGAAACTCCAAAAGAATGTTTTGCATGGACTGTTGACCCAACAGATGCACAGGACA
AAGAAGAATACGTAGAAATTGACTTCGAAGAAGGGGTTCCTGTAGCAATCAACGGGGATGCATTGGAAGCAGTTTCATTA
ATAAGAAAAGTAAACGAAATTGCAGGAAGAAATGGCGTTGGAAGAGTAGATATTGTAGAAGATAGAGTTTTAGGCTTAAA
ATCAAGAGAAAACTATGAATGCCCTGGTGCAATGCTTTTAATAACTGCACACAAAGCTCTCGAACAGCTCGTTTTAACCA
GAGAAGAACTCGTATTCAAAGAAATGGTTGATTCAAAATACGCAGATTTAGTATACAAAGGACTCTGGCACGAACCATTA
AGACTCGATTTAGATGCGTTCATTGATAAAACGCAAACAAGAATGAACGGAAAAGTAGTTTTAAAACTCTACAAAGGTTC
AATGAGAATCGCTGGAAGAGAAAGTAAAGATGCAATCTACAACGAAGAAATGGTTTCATTTGAAAACAAAGAAATGGACC
AGAGAGAAATTGTCGGAATGGTTAAATTCCACGGTCTCCAAGCAGCAATCTATGAAGGATTAACAAGAAAATAA

Upstream 100 bases:

>100_bases
ATAAAGTTTATAACTATTTAGATAATATATTGAATAAATTTTAGATTACAATTTATTTACATCAAAAATAGAACGGAAAA
ATAAAACACAGGTGATAATC

Downstream 100 bases:

>100_bases
TTACTTTTTTAATTATTTTTTTTAATATTTCTCATTTTAAAAATTTATTTTAAATTTCCACGTGATATTATGAACATTTT
TACTTCAAAAGGTACAATAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQEKIAVLAYSGGLDTSCCLKLLEDKYDYKVISVAVDVGQPEDDLKEPEEKAKKFGVLKHYTIDAKEEFAKDYIFRAIKA
NALYEGYPLSTALARPLIAIKIAELAQEVGASAISHGCTGKGNDQFRFESVMRAKTPEIEIVAPIRDLNLTRTEEIAYAK
EKGIPVPVDLEKPFSIDENLWGRSIEGGILEDPMTETPKECFAWTVDPTDAQDKEEYVEIDFEEGVPVAINGDALEAVSL
IRKVNEIAGRNGVGRVDIVEDRVLGLKSRENYECPGAMLLITAHKALEQLVLTREELVFKEMVDSKYADLVYKGLWHEPL
RLDLDAFIDKTQTRMNGKVVLKLYKGSMRIAGRESKDAIYNEEMVSFENKEMDQREIVGMVKFHGLQAAIYEGLTRK

Sequences:

>Translated_397_residues
MQEKIAVLAYSGGLDTSCCLKLLEDKYDYKVISVAVDVGQPEDDLKEPEEKAKKFGVLKHYTIDAKEEFAKDYIFRAIKA
NALYEGYPLSTALARPLIAIKIAELAQEVGASAISHGCTGKGNDQFRFESVMRAKTPEIEIVAPIRDLNLTRTEEIAYAK
EKGIPVPVDLEKPFSIDENLWGRSIEGGILEDPMTETPKECFAWTVDPTDAQDKEEYVEIDFEEGVPVAINGDALEAVSL
IRKVNEIAGRNGVGRVDIVEDRVLGLKSRENYECPGAMLLITAHKALEQLVLTREELVFKEMVDSKYADLVYKGLWHEPL
RLDLDAFIDKTQTRMNGKVVLKLYKGSMRIAGRESKDAIYNEEMVSFENKEMDQREIVGMVKFHGLQAAIYEGLTRK
>Mature_397_residues
MQEKIAVLAYSGGLDTSCCLKLLEDKYDYKVISVAVDVGQPEDDLKEPEEKAKKFGVLKHYTIDAKEEFAKDYIFRAIKA
NALYEGYPLSTALARPLIAIKIAELAQEVGASAISHGCTGKGNDQFRFESVMRAKTPEIEIVAPIRDLNLTRTEEIAYAK
EKGIPVPVDLEKPFSIDENLWGRSIEGGILEDPMTETPKECFAWTVDPTDAQDKEEYVEIDFEEGVPVAINGDALEAVSL
IRKVNEIAGRNGVGRVDIVEDRVLGLKSRENYECPGAMLLITAHKALEQLVLTREELVFKEMVDSKYADLVYKGLWHEPL
RLDLDAFIDKTQTRMNGKVVLKLYKGSMRIAGRESKDAIYNEEMVSFENKEMDQREIVGMVKFHGLQAAIYEGLTRK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=41.1910669975186, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=41.1910669975186, Blast_Score=279, Evalue=3e-75,
Organism=Escherichia coli, GI1789563, Length=381, Percent_Identity=27.0341207349081, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=372, Percent_Identity=41.1290322580645, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI21358151, Length=383, Percent_Identity=42.0365535248042, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_METMP (P61527)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_987193.1
- ProteinModelPortal:   P61527
- SMR:   P61527
- GeneID:   2761944
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0073
- NMPDR:   fig|267377.1.peg.73
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK13820
- BioCyc:   MMAR267377:MMP0073-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44581; Mature: 44581

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 175-175 BINDING 184-184 BINDING 260-260 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKIAVLAYSGGLDTSCCLKLLEDKYDYKVISVAVDVGQPEDDLKEPEEKAKKFGVLKH
CCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHCCHHHHHHHCCCHHE
YTIDAKEEFAKDYIFRAIKANALYEGYPLSTALARPLIAIKIAELAQEVGASAISHGCTG
EECCCHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCC
KGNDQFRFESVMRAKTPEIEIVAPIRDLNLTRTEEIAYAKEKGIPVPVDLEKPFSIDENL
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WGRSIEGGILEDPMTETPKECFAWTVDPTDAQDKEEYVEIDFEEGVPVAINGDALEAVSL
CCCCCCCCCCCCCCCCCCHHHEEEECCCCCCCCCHHEEEEEECCCCCEEECCHHHHHHHH
IRKVNEIAGRNGVGRVDIVEDRVLGLKSRENYECPGAMLLITAHKALEQLVLTREELVFK
HHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
EMVDSKYADLVYKGLWHEPLRLDLDAFIDKTQTRMNGKVVLKLYKGSMRIAGRESKDAIY
HHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCCCHHH
NEEMVSFENKEMDQREIVGMVKFHGLQAAIYEGLTRK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQEKIAVLAYSGGLDTSCCLKLLEDKYDYKVISVAVDVGQPEDDLKEPEEKAKKFGVLKH
CCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHCCHHHHHHHCCCHHE
YTIDAKEEFAKDYIFRAIKANALYEGYPLSTALARPLIAIKIAELAQEVGASAISHGCTG
EECCCHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCC
KGNDQFRFESVMRAKTPEIEIVAPIRDLNLTRTEEIAYAKEKGIPVPVDLEKPFSIDENL
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WGRSIEGGILEDPMTETPKECFAWTVDPTDAQDKEEYVEIDFEEGVPVAINGDALEAVSL
CCCCCCCCCCCCCCCCCCHHHEEEECCCCCCCCCHHEEEEEECCCCCEEECCHHHHHHHH
IRKVNEIAGRNGVGRVDIVEDRVLGLKSRENYECPGAMLLITAHKALEQLVLTREELVFK
HHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
EMVDSKYADLVYKGLWHEPLRLDLDAFIDKTQTRMNGKVVLKLYKGSMRIAGRESKDAIY
HHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCCCHHH
NEEMVSFENKEMDQREIVGMVKFHGLQAAIYEGLTRK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA