Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 45357628

GI number: 45357628

Start: 77116

End: 78342

Strand: Direct

Name: amtB [C]

Synonym: MMP0065

Alternate gene names: 45357628

Gene position: 77116-78342 (Clockwise)

Preceding gene: 45357627

Following gene: 45357629

Centisome position: 4.64

GC content: 41.56

Gene sequence:

>1227_bases
ATGGCAACGGCAGATTTATTTGCTGACCCCACAAACATCGTAACAGGGCTCAGTACATTGGCAACTTCTGGAGATGTAAT
GTTCTTAGTATTGATGGGAGTACTTGTGTTTTCAATGCAGTTAGGATTTGCACTTCTCGAAGGCGGTCAGGTCAGAAAGA
AAAATGTTAACAACGTAATGATGAAAAACATGGCAGACTGGTTAATTGGAGCAGTTTCATGGCTTTTCATAGGCGGTGTT
CTTTCAGTTTCACTTGTTCCTGCAGACTTCTTCAGCTGGTGGGGACAAATATTTACAACAAACTTTGCAAATAATGGAAT
AGACTTAGCAAACTGGTTCTTTGGACTGGTATTCGCAGCAACTGCAGCTACAATCGTATCAGGTGGTGTTGCAGAAAGGA
TTAAATTTAAATCATACTTATTAATGTCAATAATCATAACTGCGATACTATACCCGCTGTTTGTGTACCTTGGACCTTGG
GGTTCAGGAATGATTCCATTCAACGATTACGCTGGAAGTTTCGTTGTACACGGTCTAGGTGGTTTCTTAGCACTTGGAGC
AATTGCGGCTCTTGGACCTAGGATTGGAAAATTCGTTAACAAAAAAGCAATTTCAATTCCAGGACACAATATCCCAATGT
CAGTATTCGGGGCATTTGTTCTTGCAATCGGATGGTACGGATTTAACGTAGGAAGTTCATTTGCAGTTGCAGATATTTCT
GGATTAGTATGTGCAACAACAACACTTGCAATGGCAGGTGGAGGTATTGGAGCACTTCTCGCTTCAAAAGATGATGTATT
ATTCACAGCAAACGGTATTGTAGCAGGTTTAGTTGCAATCTGTTCAGGAACGGATGTTGTAAGCCCAATAGGAGCATTAA
TTATCGGGCTTGTTGCAGGAATTCAGGTACCAATCGTCTACAAAATCGTTGAAAAAATGGGATTGGATGACGTATGTGGT
GTAATTCCAGTACACGGTACAGCAGGTATTTTAGGAGGTATCTTAACAGGTGTCTTTGGAATGGCTGCTCTTGGTGGTGC
TGGTGGAGTAAGTTTGGTAAACCAGCTTATTGCAGCAGGATTAACAGTGGTATACGGTACAGGATTAGGATTCGGTCTTG
GAAAATTAGTTGGTGGATTCACTGGTGGACTCAGAGTTACTGAAGAAGAAGAAAAAATGGGTCTCGACCTTGCAGAACAC
AAATTGCCAGCTTACCCTGACGAATAA

Upstream 100 bases:

>100_bases
GAAAAATCTTTATTATTCCAGTAGAAGACGCAGTAAGAATTAGAACAGGCGAATCAGGCATAAAAGCTATTTAAATTAAA
AATAAAATCAGGTGAAAATT

Downstream 100 bases:

>100_bases
TTAATTTTTCCTTTTTTTCATTGAAAAATAGATACTATTATATATTTTTCAGAATATATTTTTTCAAAATGTTGAATATA
TTTACTATTTTGGCGGTGTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MATADLFADPTNIVTGLSTLATSGDVMFLVLMGVLVFSMQLGFALLEGGQVRKKNVNNVMMKNMADWLIGAVSWLFIGGV
LSVSLVPADFFSWWGQIFTTNFANNGIDLANWFFGLVFAATAATIVSGGVAERIKFKSYLLMSIIITAILYPLFVYLGPW
GSGMIPFNDYAGSFVVHGLGGFLALGAIAALGPRIGKFVNKKAISIPGHNIPMSVFGAFVLAIGWYGFNVGSSFAVADIS
GLVCATTTLAMAGGGIGALLASKDDVLFTANGIVAGLVAICSGTDVVSPIGALIIGLVAGIQVPIVYKIVEKMGLDDVCG
VIPVHGTAGILGGILTGVFGMAALGGAGGVSLVNQLIAAGLTVVYGTGLGFGLGKLVGGFTGGLRVTEEEEKMGLDLAEH
KLPAYPDE

Sequences:

>Translated_408_residues
MATADLFADPTNIVTGLSTLATSGDVMFLVLMGVLVFSMQLGFALLEGGQVRKKNVNNVMMKNMADWLIGAVSWLFIGGV
LSVSLVPADFFSWWGQIFTTNFANNGIDLANWFFGLVFAATAATIVSGGVAERIKFKSYLLMSIIITAILYPLFVYLGPW
GSGMIPFNDYAGSFVVHGLGGFLALGAIAALGPRIGKFVNKKAISIPGHNIPMSVFGAFVLAIGWYGFNVGSSFAVADIS
GLVCATTTLAMAGGGIGALLASKDDVLFTANGIVAGLVAICSGTDVVSPIGALIIGLVAGIQVPIVYKIVEKMGLDDVCG
VIPVHGTAGILGGILTGVFGMAALGGAGGVSLVNQLIAAGLTVVYGTGLGFGLGKLVGGFTGGLRVTEEEEKMGLDLAEH
KLPAYPDE
>Mature_407_residues
ATADLFADPTNIVTGLSTLATSGDVMFLVLMGVLVFSMQLGFALLEGGQVRKKNVNNVMMKNMADWLIGAVSWLFIGGVL
SVSLVPADFFSWWGQIFTTNFANNGIDLANWFFGLVFAATAATIVSGGVAERIKFKSYLLMSIIITAILYPLFVYLGPWG
SGMIPFNDYAGSFVVHGLGGFLALGAIAALGPRIGKFVNKKAISIPGHNIPMSVFGAFVLAIGWYGFNVGSSFAVADISG
LVCATTTLAMAGGGIGALLASKDDVLFTANGIVAGLVAICSGTDVVSPIGALIIGLVAGIQVPIVYKIVEKMGLDDVCGV
IPVHGTAGILGGILTGVFGMAALGGAGGVSLVNQLIAAGLTVVYGTGLGFGLGKLVGGFTGGLRVTEEEEKMGLDLAEHK
LPAYPDE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=30.3827751196172, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=31.685393258427, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=31.6397228637413, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI32564042, Length=420, Percent_Identity=30.952380952381, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI133931100, Length=427, Percent_Identity=28.3372365339578, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17563228, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325396, Length=413, Percent_Identity=26.1501210653753, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321559, Length=407, Percent_Identity=26.7813267813268, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=399, Percent_Identity=23.0576441102757, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078314, Length=435, Percent_Identity=30.8045977011494, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41947; Mature: 41816

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHEEECCCHHHHHHHHHCCCCCCEECCHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure 
ATADLFADPTNIVTGLSTLATSGDVMFLVLMGVLVFSMQLGFALLEGGQVRKKNVNNVM
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MKNMADWLIGAVSWLFIGGVLSVSLVPADFFSWWGQIFTTNFANNGIDLANWFFGLVFAA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TAATIVSGGVAERIKFKSYLLMSIIITAILYPLFVYLGPWGSGMIPFNDYAGSFVVHGLG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GFLALGAIAALGPRIGKFVNKKAISIPGHNIPMSVFGAFVLAIGWYGFNVGSSFAVADIS
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
GLVCATTTLAMAGGGIGALLASKDDVLFTANGIVAGLVAICSGTDVVSPIGALIIGLVAG
HHHHHHHHHHHHCCCHHHHEECCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IQVPIVYKIVEKMGLDDVCGVIPVHGTAGILGGILTGVFGMAALGGAGGVSLVNQLIAAG
CHHHHHHHHHHHCCCCHHHCCEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
LTVVYGTGLGFGLGKLVGGFTGGLRVTEEEEKMGLDLAEHKLPAYPDE
HHHEEECCCHHHHHHHHHCCCCCCEECCHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]