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The map label for this gene is ygcA [C]

Identifier: 42523529

GI number: 42523529

Start: 1962854

End: 1964020

Strand: Direct

Name: ygcA [C]

Synonym: Bd2056

Alternate gene names: 42523529

Gene position: 1962854-1964020 (Clockwise)

Preceding gene: 42523527

Following gene: 42523533

Centisome position: 51.89

GC content: 52.96

Gene sequence:

>1167_bases
ATGCCGAACTTTCAGAAATTCGAAACCTTTGACAAACTGACCACCGTGCCTTGTCCCTACGCCCCGGACTGTGGCGGATG
TCAGCATATCGGCGTGCCTTACGGACATCAGGCTCAGAACAAAATAAACGACCTTTCAGGGCTGTTTACCGCCGCAGAGG
TCGCATTTCCAAAGCCCCTCAAAGTTCTTTCTGCCGGCCCCGCCCATTTGCGTGACCGTTTGGATTTCAGCCTGCAAGAC
GGCCGCCTGGGGCTTTATCGCACGGATCGTCATGAGATTCTGGATATCGAAGTCTGCGCCCAGCTCAGTCCGGCACTGCA
AGGATGGCTGTCTGAGTTTAGAAAAATCCAGTGGCCCTTTAAGCGTGGCTCGTTCCGACTGCGCGTCGGACCGGAAGGAC
AGCGTGGACTGTGGATTGACCTGGCCAATGTGGATATCAAAACCCTTTTGGATGAGCAGAACATCCTGCGTTCTTTGCAA
CAGCAGGCTTTTGTGGAAATCGGCCAGCGCCGTAAAGTTCCGGTTTGGACCGGAAATGAATTTAAACTGCGCGATCCCGA
GCACCACGTGTGGTTCCAGTCCTGGATGAACGACATTCCCGTAAATCTGTACTGTCAGGTGGCGAGCTTCACTCAGCCCA
GCCACACCGCCAATAAGATCATTTGCGATGTGATTTACGATTGGGTGAAACGCTATAAAGCCCAGCGCCTGATCGAGTTC
GGCTCTGGCATCGGCAACCTGACCTTCCCGGCTTTGGCGGGGGCAGAGAGTGTTCTGGCGTGTGAAATCGACGAACTGTC
TCTGCAAGGCCTGGAGCGCTCTTTAAACGAGCTTCCGGCAAGCATGTCCCATCTGAAGGATCGCGTGACTATTTATCGCA
GGGACTTCCAGAAAAAACTGACTCAGGATTTTTCTCAGTTTGATGGGGTGCTGGCGAATCCACCAAGATCGGGCCTGATG
GGCTTTTTGAATCCGCTAAAAAGCCTTGCGCCCGAACAGCGGCCCGAGTTCTTTATTTATATGTCCTGTTATCCTGAATC
CATGGCCCGGGATCTGGTGACCCTGCAGGAATGCGGATACCGCATGCAAGAGGTCTATATCGTCGACCAATTCCCGCAAA
CGGATCACTATGAGGTTTTGGGACTACTTCAAAGAGAAAAGGCGTAA

Upstream 100 bases:

>100_bases
ATGATCATGCTAGCAAGCAATAAAATTCTCACCATTCCCCCATTAGTTTTGATAAGATAGGTTTTTCTTATAACAGCGAA
ATTGCCGGGGCAAGCACTCA

Downstream 100 bases:

>100_bases
GCCTGAAAGCGCGCCAGCCAGATCTTTTCCGGCCAACAGTGAAAGCAGCGGCAACGTCACAATTCCGGTGATTAAAATCA
AGGAACTGCGCAAAGTCACA

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPNFQKFETFDKLTTVPCPYAPDCGGCQHIGVPYGHQAQNKINDLSGLFTAAEVAFPKPLKVLSAGPAHLRDRLDFSLQD
GRLGLYRTDRHEILDIEVCAQLSPALQGWLSEFRKIQWPFKRGSFRLRVGPEGQRGLWIDLANVDIKTLLDEQNILRSLQ
QQAFVEIGQRRKVPVWTGNEFKLRDPEHHVWFQSWMNDIPVNLYCQVASFTQPSHTANKIICDVIYDWVKRYKAQRLIEF
GSGIGNLTFPALAGAESVLACEIDELSLQGLERSLNELPASMSHLKDRVTIYRRDFQKKLTQDFSQFDGVLANPPRSGLM
GFLNPLKSLAPEQRPEFFIYMSCYPESMARDLVTLQECGYRMQEVYIVDQFPQTDHYEVLGLLQREKA

Sequences:

>Translated_388_residues
MPNFQKFETFDKLTTVPCPYAPDCGGCQHIGVPYGHQAQNKINDLSGLFTAAEVAFPKPLKVLSAGPAHLRDRLDFSLQD
GRLGLYRTDRHEILDIEVCAQLSPALQGWLSEFRKIQWPFKRGSFRLRVGPEGQRGLWIDLANVDIKTLLDEQNILRSLQ
QQAFVEIGQRRKVPVWTGNEFKLRDPEHHVWFQSWMNDIPVNLYCQVASFTQPSHTANKIICDVIYDWVKRYKAQRLIEF
GSGIGNLTFPALAGAESVLACEIDELSLQGLERSLNELPASMSHLKDRVTIYRRDFQKKLTQDFSQFDGVLANPPRSGLM
GFLNPLKSLAPEQRPEFFIYMSCYPESMARDLVTLQECGYRMQEVYIVDQFPQTDHYEVLGLLQREKA
>Mature_387_residues
PNFQKFETFDKLTTVPCPYAPDCGGCQHIGVPYGHQAQNKINDLSGLFTAAEVAFPKPLKVLSAGPAHLRDRLDFSLQDG
RLGLYRTDRHEILDIEVCAQLSPALQGWLSEFRKIQWPFKRGSFRLRVGPEGQRGLWIDLANVDIKTLLDEQNILRSLQQ
QAFVEIGQRRKVPVWTGNEFKLRDPEHHVWFQSWMNDIPVNLYCQVASFTQPSHTANKIICDVIYDWVKRYKAQRLIEFG
SGIGNLTFPALAGAESVLACEIDELSLQGLERSLNELPASMSHLKDRVTIYRRDFQKKLTQDFSQFDGVLANPPRSGLMG
FLNPLKSLAPEQRPEFFIYMSCYPESMARDLVTLQECGYRMQEVYIVDQFPQTDHYEVLGLLQREKA

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789148, Length=388, Percent_Identity=25, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787083, Length=195, Percent_Identity=29.2307692307692, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2056_BDEBA (Q6MLF5)

Other databases:

- EMBL:   BX842651
- RefSeq:   NP_968909.1
- ProteinModelPortal:   Q6MLF5
- SMR:   Q6MLF5
- GeneID:   2734002
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd2056
- NMPDR:   fig|264462.1.peg.1880
- PhylomeDB:   Q6MLF5
- BioCyc:   BBAC264462:BD2056-MONOMER
- InterPro:   IPR020596
- InterPro:   IPR010280

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: NA

Molecular weight: Translated: 44444; Mature: 44313

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01131 RRNA_A_DIMETH; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 343-343 BINDING 212-212 BINDING 262-262 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNFQKFETFDKLTTVPCPYAPDCGGCQHIGVPYGHQAQNKINDLSGLFTAAEVAFPKPL
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
KVLSAGPAHLRDRLDFSLQDGRLGLYRTDRHEILDIEVCAQLSPALQGWLSEFRKIQWPF
HHHCCCHHHHHHHCCCEECCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCC
KRGSFRLRVGPEGQRGLWIDLANVDIKTLLDEQNILRSLQQQAFVEIGQRRKVPVWTGNE
CCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCE
FKLRDPEHHVWFQSWMNDIPVNLYCQVASFTQPSHTANKIICDVIYDWVKRYKAQRLIEF
EEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GSGIGNLTFPALAGAESVLACEIDELSLQGLERSLNELPASMSHLKDRVTIYRRDFQKKL
CCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TQDFSQFDGVLANPPRSGLMGFLNPLKSLAPEQRPEFFIYMSCYPESMARDLVTLQECGY
HHHHHHHCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
RMQEVYIVDQFPQTDHYEVLGLLQREKA
CEEEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PNFQKFETFDKLTTVPCPYAPDCGGCQHIGVPYGHQAQNKINDLSGLFTAAEVAFPKPL
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
KVLSAGPAHLRDRLDFSLQDGRLGLYRTDRHEILDIEVCAQLSPALQGWLSEFRKIQWPF
HHHCCCHHHHHHHCCCEECCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCC
KRGSFRLRVGPEGQRGLWIDLANVDIKTLLDEQNILRSLQQQAFVEIGQRRKVPVWTGNE
CCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCE
FKLRDPEHHVWFQSWMNDIPVNLYCQVASFTQPSHTANKIICDVIYDWVKRYKAQRLIEF
EEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GSGIGNLTFPALAGAESVLACEIDELSLQGLERSLNELPASMSHLKDRVTIYRRDFQKKL
CCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TQDFSQFDGVLANPPRSGLMGFLNPLKSLAPEQRPEFFIYMSCYPESMARDLVTLQECGY
HHHHHHHCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
RMQEVYIVDQFPQTDHYEVLGLLQREKA
CEEEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA