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The map label for this gene is sufS [H]

Identifier: 42523416

GI number: 42523416

Start: 1850642

End: 1851847

Strand: Direct

Name: sufS [H]

Synonym: Bd1938

Alternate gene names: 42523416

Gene position: 1850642-1851847 (Clockwise)

Preceding gene: 42523411

Following gene: 42523417

Centisome position: 48.92

GC content: 52.07

Gene sequence:

>1206_bases
ATGATACAGACCAAGGTCCTTCATTATTTCGATCACAACGCCACGACTCCGGCGTGCAAAGAGGTTTTGGAATCTCTGCC
CGAGTTCGCGCAAGCTTGGGGGAACCCAAGTTCCATCCATTGGGGTGGTCGGCAGCCGAAGAATATTCTGCGTGACGCGC
GCAAGGCTGTGGCAGAAATGGTGAATGCAAGTCCGCTGGAAATCATTTTCACTTCCGGTGGCAGTGAAGCCAACAACACC
GTTTTGAAAGGTCTGCTGGAATACTACCAGACAGCACAGTTCCTGACTCCGGAACAGCGCAAACGCACTCACTTCATGAG
TTCTGAAGTGGAGCACCCAAGCATCATGAAAACCATGCAGCACCTGAAAGATCTGGGCGCACGCGTGGATTTCATTCCGG
TGAATCGTCAGGGGCAGATCGATCTGAAATTCTATGAAGAACATCTGAGCGAAGAGACCGCCCTGGTGTCCGTGATGTTT
GCCAATAACGAAACCGGCACATTGTTCCCGATCAAAGAGATGGCCGAGATGGCACATAAAAAAGGCGCGCTCTTCCACAC
GGATGCAGTTCAGGCCTTCGGTAAAGTGCCGGTGAACCTGAAAGAACTGAATGTCGACTTCGCTTCTTTCTCTGGTCATA
AATTCTATTCCGTCAAAGGAACTGGGGTTTTGTATGCGAAGAAGGGTAACAACGTCACACCTTTGATTCACGGTGGAGCG
CAGGAAAGACATCGTCGTGGTGGCACTGAAAACACACTGGGCATTGGTGCTCTGGGTGTGGTGGCCAAACGTGCGCCGAT
GATTGCGGAAAAAGCCAAAGCCTTGGCTGTTCTTCGCGATCACATGGAAGCGCGCATTCTTTCTGAAATTTCCGAAGTGA
CGGTGACTGCCGGGGAAACTCCGCGTTTGCCGAACACAAGTTCATTGGTACTGAAGGGGGCTGACGGGGAAACCATGTTG
ATGTCTTTGGATATCAAGGGCTATGCGGTTTCCACCGGGGCTGCTTGTTCTTCCGGGAATCCCGAGCCAAGCCCGGTGCT
TCTGGCCATGGGTCTGACCCGCGATGAAGCGCAGAACAGCCTGCGTGTCAGTCTTGGCTGGGACAGCACTCTGGAGCAGG
TGGATTCGTTTGTAGATACATTGAAAACAGTAGTGGATCGCTTGCGATCCTTGAACGACGACAAAGGAGAGACTTGTCAT
GTCTAA

Upstream 100 bases:

>100_bases
GTAGAAATTTCCCGATCTGTTAACGCAGTGGACACCCTAAAAGGGCAAATTATCTTAGAGGTTCTCAATACTTAAGAACT
GTGATAGGTTCCGGCAGCTT

Downstream 100 bases:

>100_bases
AGGGAGAGTCCTTGTTGCTATGAGCGGAGGCGTGGACAGTTCCGCCGCGGCTGCGCTCTTGGTCGAACAGGGTTATGAAG
TTATTGGAGCCACGATGCAG

Product: cysteine desulphurase involved in Fe-S clsuter assembly

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MIQTKVLHYFDHNATTPACKEVLESLPEFAQAWGNPSSIHWGGRQPKNILRDARKAVAEMVNASPLEIIFTSGGSEANNT
VLKGLLEYYQTAQFLTPEQRKRTHFMSSEVEHPSIMKTMQHLKDLGARVDFIPVNRQGQIDLKFYEEHLSEETALVSVMF
ANNETGTLFPIKEMAEMAHKKGALFHTDAVQAFGKVPVNLKELNVDFASFSGHKFYSVKGTGVLYAKKGNNVTPLIHGGA
QERHRRGGTENTLGIGALGVVAKRAPMIAEKAKALAVLRDHMEARILSEISEVTVTAGETPRLPNTSSLVLKGADGETML
MSLDIKGYAVSTGAACSSGNPEPSPVLLAMGLTRDEAQNSLRVSLGWDSTLEQVDSFVDTLKTVVDRLRSLNDDKGETCH
V

Sequences:

>Translated_401_residues
MIQTKVLHYFDHNATTPACKEVLESLPEFAQAWGNPSSIHWGGRQPKNILRDARKAVAEMVNASPLEIIFTSGGSEANNT
VLKGLLEYYQTAQFLTPEQRKRTHFMSSEVEHPSIMKTMQHLKDLGARVDFIPVNRQGQIDLKFYEEHLSEETALVSVMF
ANNETGTLFPIKEMAEMAHKKGALFHTDAVQAFGKVPVNLKELNVDFASFSGHKFYSVKGTGVLYAKKGNNVTPLIHGGA
QERHRRGGTENTLGIGALGVVAKRAPMIAEKAKALAVLRDHMEARILSEISEVTVTAGETPRLPNTSSLVLKGADGETML
MSLDIKGYAVSTGAACSSGNPEPSPVLLAMGLTRDEAQNSLRVSLGWDSTLEQVDSFVDTLKTVVDRLRSLNDDKGETCH
V
>Mature_401_residues
MIQTKVLHYFDHNATTPACKEVLESLPEFAQAWGNPSSIHWGGRQPKNILRDARKAVAEMVNASPLEIIFTSGGSEANNT
VLKGLLEYYQTAQFLTPEQRKRTHFMSSEVEHPSIMKTMQHLKDLGARVDFIPVNRQGQIDLKFYEEHLSEETALVSVMF
ANNETGTLFPIKEMAEMAHKKGALFHTDAVQAFGKVPVNLKELNVDFASFSGHKFYSVKGTGVLYAKKGNNVTPLIHGGA
QERHRRGGTENTLGIGALGVVAKRAPMIAEKAKALAVLRDHMEARILSEISEVTVTAGETPRLPNTSSLVLKGADGETML
MSLDIKGYAVSTGAACSSGNPEPSPVLLAMGLTRDEAQNSLRVSLGWDSTLEQVDSFVDTLKTVVDRLRSLNDDKGETCH
V

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI156713448, Length=410, Percent_Identity=36.5853658536585, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI32307132, Length=391, Percent_Identity=35.2941176470588, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=36.1323155216285, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1787970, Length=406, Percent_Identity=26.6009852216749, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789175, Length=191, Percent_Identity=26.7015706806283, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=396, Percent_Identity=35.1010101010101, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17533177, Length=331, Percent_Identity=31.1178247734139, Blast_Score=154, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=36.4102564102564, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI20129463, Length=391, Percent_Identity=36.5728900255754, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43858; Mature: 43858

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTKVLHYFDHNATTPACKEVLESLPEFAQAWGNPSSIHWGGRQPKNILRDARKAVAEM
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VNASPLEIIFTSGGSEANNTVLKGLLEYYQTAQFLTPEQRKRTHFMSSEVEHPSIMKTMQ
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHH
HLKDLGARVDFIPVNRQGQIDLKFYEEHLSEETALVSVMFANNETGTLFPIKEMAEMAHK
HHHHHCCEEEEEECCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCEEEHHHHHHHHHH
KGALFHTDAVQAFGKVPVNLKELNVDFASFSGHKFYSVKGTGVLYAKKGNNVTPLIHGGA
CCCEEEHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCEEECCH
QERHRRGGTENTLGIGALGVVAKRAPMIAEKAKALAVLRDHMEARILSEISEVTVTAGET
HHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
PRLPNTSSLVLKGADGETMLMSLDIKGYAVSTGAACSSGNPEPSPVLLAMGLTRDEAQNS
CCCCCCCCEEEECCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCEEEEECCCHHHCCCC
LRVSLGWDSTLEQVDSFVDTLKTVVDRLRSLNDDKGETCHV
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIQTKVLHYFDHNATTPACKEVLESLPEFAQAWGNPSSIHWGGRQPKNILRDARKAVAEM
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VNASPLEIIFTSGGSEANNTVLKGLLEYYQTAQFLTPEQRKRTHFMSSEVEHPSIMKTMQ
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHH
HLKDLGARVDFIPVNRQGQIDLKFYEEHLSEETALVSVMFANNETGTLFPIKEMAEMAHK
HHHHHCCEEEEEECCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCEEEHHHHHHHHHH
KGALFHTDAVQAFGKVPVNLKELNVDFASFSGHKFYSVKGTGVLYAKKGNNVTPLIHGGA
CCCEEEHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCEEECCH
QERHRRGGTENTLGIGALGVVAKRAPMIAEKAKALAVLRDHMEARILSEISEVTVTAGET
HHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
PRLPNTSSLVLKGADGETMLMSLDIKGYAVSTGAACSSGNPEPSPVLLAMGLTRDEAQNS
CCCCCCCCEEEECCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCEEEEECCCHHHCCCC
LRVSLGWDSTLEQVDSFVDTLKTVVDRLRSLNDDKGETCHV
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]