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The map label for this gene is yhjG [H]

Identifier: 42522969

GI number: 42522969

Start: 1377260

End: 1378672

Strand: Direct

Name: yhjG [H]

Synonym: Bd1456

Alternate gene names: 42522969

Gene position: 1377260-1378672 (Clockwise)

Preceding gene: 42522968

Following gene: 42522971

Centisome position: 36.41

GC content: 50.32

Gene sequence:

>1413_bases
ATGAGCCCGCAAGTTGTTGATCTTGTCATTGTCGGCGCGGGACCGGTCGGATTGATGTGTGCTTATCTTGCGAAGCTTTG
CGGGATGAGCACTGTGATTGTTGATAAATCCGAGGGACCACTGCAAGTGGGGCGCGCGGATGCATTGAACGCCAGGACTT
TGCAGCTTTTGGAAGTGGGTAAATTGTTTGACCAGCTTTATCCGTTGGGAAAGCCCTGCAATACAAGTTCTGTATGGGCT
GATGGAAAGTTTCTTTCCCGCCAGTCTTCTTGGTGGGACGAGCTTGAGGGGTGTTTTCATAAGCACTTTCTGATGCTGGG
GCAGTCTTTTGTTGAAAAGCTGCTGGATGAAAAACTGACCCTTTCAGGCGCCGCTGTTCGCCGTTCCACGGTGATTAAAG
ATGTCGAGGTTCTTCAGGACAACTGCCTGACGACGTTGTCTTCCGGTGAAAAGATACAATCCCGTTATGTTATTGGTGCC
GATGGTTCCCGCTCGTTTGTACGTGAAAAGTTCAAGGTGCCTTTTGAAATCGTCAGACCCCAGATTGTCTGGGCGGTGCT
TGACGGTGTCGTTGAAACGGACTTTCCAAAAGTGCCCGAAATCATCGTCTTTCAGGCAGACACTTCGGACGTGGCGTGGA
TTCCTCGCGAAGGGGACATTGATCGTTTCTATGTCCGAATGGACACCACAGAGTTTACTTTGGAAGAAGCTGTTGCAAAA
ATCAATCGTGCGATCAAGCCCCATGTTCTGGGCTTTAAAAAGATGGAGTGGTTTTCACAGTTTTCGGTGAAGGAGTCCGT
GGCTGAAAAGTTCTTCATTCAGGATCGGGTGTTTCTTGTTGGCGATGCCAGCCATATTCACTCGGTCAACGGCGGGCAGG
GGCTGAACACGGGGCTGGCTGATGCTTTCAATCTGATCTGGAAGTTGAACATGACTTTGAATCACGGGGCTTCGTTGGAT
CTGCTGCAAAGCTACGAATCTGAACGCAAACCCGTTGCTTTAAGTGTCATCGAGGCTTCGGGGGAACTTGTTCGTTCAAC
CAAGTATTCTGCGAATGGGACTCATGCCGAGGATTATGTGCGCATCGTGCAAAAACGCGCGGGAAATATCACGGGCATGG
GGATTCGTTACGCAGGTGAGGGGCTTATCGGGTCCCGGTTGTATGACTCTGAGGTCCGCTTCGGGTCCGAGACGGTTCGG
CTGTACTCTTTGTTGAATTATTCCAAGTATACGTTGTTTGTCTTTGGGGATGTGGAGCTTGATTTTGAGTTGCCGGAGTA
TGTTCAGCTTGTTGTCGTTGGCTCTTCGGATTCTGTCTATGTCGGGCAGGCGGTGTTGGTGAGACCGGATGCTTATATTG
AATCAACGGTCTCGTTGGGTCGGGCTCGGGAGCTTCTGTCGTCTTTTATTTAG

Upstream 100 bases:

>100_bases
ACGGACGCGAAGCGTTTACCTCGAAATCCCCGCGTCACCTGCAGCGAGTGCAGGTGCAAAGCAGTCCGCCGTTTGATAAC
CCGGGCCTGGAATCGTACAA

Downstream 100 bases:

>100_bases
AGGTCTAAATCCAGTTCGTAGATGCCTCGGGCGGCGAGGACTTCCTGGGTGGAGTTTAGTTTTCTTACTTCGGTGAGTTC
CCAGGAGTAGTAGCCGGGTT

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSPQVVDLVIVGAGPVGLMCAYLAKLCGMSTVIVDKSEGPLQVGRADALNARTLQLLEVGKLFDQLYPLGKPCNTSSVWA
DGKFLSRQSSWWDELEGCFHKHFLMLGQSFVEKLLDEKLTLSGAAVRRSTVIKDVEVLQDNCLTTLSSGEKIQSRYVIGA
DGSRSFVREKFKVPFEIVRPQIVWAVLDGVVETDFPKVPEIIVFQADTSDVAWIPREGDIDRFYVRMDTTEFTLEEAVAK
INRAIKPHVLGFKKMEWFSQFSVKESVAEKFFIQDRVFLVGDASHIHSVNGGQGLNTGLADAFNLIWKLNMTLNHGASLD
LLQSYESERKPVALSVIEASGELVRSTKYSANGTHAEDYVRIVQKRAGNITGMGIRYAGEGLIGSRLYDSEVRFGSETVR
LYSLLNYSKYTLFVFGDVELDFELPEYVQLVVVGSSDSVYVGQAVLVRPDAYIESTVSLGRARELLSSFI

Sequences:

>Translated_470_residues
MSPQVVDLVIVGAGPVGLMCAYLAKLCGMSTVIVDKSEGPLQVGRADALNARTLQLLEVGKLFDQLYPLGKPCNTSSVWA
DGKFLSRQSSWWDELEGCFHKHFLMLGQSFVEKLLDEKLTLSGAAVRRSTVIKDVEVLQDNCLTTLSSGEKIQSRYVIGA
DGSRSFVREKFKVPFEIVRPQIVWAVLDGVVETDFPKVPEIIVFQADTSDVAWIPREGDIDRFYVRMDTTEFTLEEAVAK
INRAIKPHVLGFKKMEWFSQFSVKESVAEKFFIQDRVFLVGDASHIHSVNGGQGLNTGLADAFNLIWKLNMTLNHGASLD
LLQSYESERKPVALSVIEASGELVRSTKYSANGTHAEDYVRIVQKRAGNITGMGIRYAGEGLIGSRLYDSEVRFGSETVR
LYSLLNYSKYTLFVFGDVELDFELPEYVQLVVVGSSDSVYVGQAVLVRPDAYIESTVSLGRARELLSSFI
>Mature_469_residues
SPQVVDLVIVGAGPVGLMCAYLAKLCGMSTVIVDKSEGPLQVGRADALNARTLQLLEVGKLFDQLYPLGKPCNTSSVWAD
GKFLSRQSSWWDELEGCFHKHFLMLGQSFVEKLLDEKLTLSGAAVRRSTVIKDVEVLQDNCLTTLSSGEKIQSRYVIGAD
GSRSFVREKFKVPFEIVRPQIVWAVLDGVVETDFPKVPEIIVFQADTSDVAWIPREGDIDRFYVRMDTTEFTLEEAVAKI
NRAIKPHVLGFKKMEWFSQFSVKESVAEKFFIQDRVFLVGDASHIHSVNGGQGLNTGLADAFNLIWKLNMTLNHGASLDL
LQSYESERKPVALSVIEASGELVRSTKYSANGTHAEDYVRIVQKRAGNITGMGIRYAGEGLIGSRLYDSEVRFGSETVRL
YSLLNYSKYTLFVFGDVELDFELPEYVQLVVVGSSDSVYVGQAVLVRPDAYIESTVSLGRARELLSSFI

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=349, Percent_Identity=27.5071633237822, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 52205; Mature: 52074

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQVVDLVIVGAGPVGLMCAYLAKLCGMSTVIVDKSEGPLQVGRADALNARTLQLLEVG
CCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHH
KLFDQLYPLGKPCNTSSVWADGKFLSRQSSWWDELEGCFHKHFLMLGQSFVEKLLDEKLT
HHHHHHCCCCCCCCCCCEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSGAAVRRSTVIKDVEVLQDNCLTTLSSGEKIQSRYVIGADGSRSFVREKFKVPFEIVRP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCEEEEECCCCHHHHHHHHCCCHHHHHH
QIVWAVLDGVVETDFPKVPEIIVFQADTSDVAWIPREGDIDRFYVRMDTTEFTLEEAVAK
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCHHHHHHHHHHH
INRAIKPHVLGFKKMEWFSQFSVKESVAEKFFIQDRVFLVGDASHIHSVNGGQGLNTGLA
HHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCCCHHH
DAFNLIWKLNMTLNHGASLDLLQSYESERKPVALSVIEASGELVRSTKYSANGTHAEDYV
HHHHHHEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCCCCCHHHHH
RIVQKRAGNITGMGIRYAGEGLIGSRLYDSEVRFGSETVRLYSLLNYSKYTLFVFGDVEL
HHHHHHCCCCEEEEEEEECCCCCCCCEECCHHHCCHHHHHHHHHHCCCEEEEEEEEEEEE
DFELPEYVQLVVVGSSDSVYVGQAVLVRPDAYIESTVSLGRARELLSSFI
CCCCCCEEEEEEECCCCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPQVVDLVIVGAGPVGLMCAYLAKLCGMSTVIVDKSEGPLQVGRADALNARTLQLLEVG
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHH
KLFDQLYPLGKPCNTSSVWADGKFLSRQSSWWDELEGCFHKHFLMLGQSFVEKLLDEKLT
HHHHHHCCCCCCCCCCCEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSGAAVRRSTVIKDVEVLQDNCLTTLSSGEKIQSRYVIGADGSRSFVREKFKVPFEIVRP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCEEEEECCCCHHHHHHHHCCCHHHHHH
QIVWAVLDGVVETDFPKVPEIIVFQADTSDVAWIPREGDIDRFYVRMDTTEFTLEEAVAK
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCHHHHHHHHHHH
INRAIKPHVLGFKKMEWFSQFSVKESVAEKFFIQDRVFLVGDASHIHSVNGGQGLNTGLA
HHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCCCHHH
DAFNLIWKLNMTLNHGASLDLLQSYESERKPVALSVIEASGELVRSTKYSANGTHAEDYV
HHHHHHEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCCCCCHHHHH
RIVQKRAGNITGMGIRYAGEGLIGSRLYDSEVRFGSETVRLYSLLNYSKYTLFVFGDVEL
HHHHHHCCCCEEEEEEEECCCCCCCCEECCHHHCCHHHHHHHHHHCCCEEEEEEEEEEEE
DFELPEYVQLVVVGSSDSVYVGQAVLVRPDAYIESTVSLGRARELLSSFI
CCCCCCEEEEEEECCCCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]