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The map label for this gene is greB [H]

Identifier: 42522667

GI number: 42522667

Start: 1054877

End: 1055428

Strand: Direct

Name: greB [H]

Synonym: Bd1119

Alternate gene names: 42522667

Gene position: 1054877-1055428 (Clockwise)

Preceding gene: 42522663

Following gene: 42522668

Centisome position: 27.89

GC content: 48.73

Gene sequence:

>552_bases
TTGGGCGATGGAATTATGGAATCAAAATCAAATTCCCGTTGGACCGGTCTGGAAGATGGTCTAGAGTCTTGCGGTATGGA
TAACAATAAGAACTACATCACCCCCGAAGGTCTGGCTAAGTTGAAGGCGGAATATCACGCGTTGATGCACGTGGAGCGGC
CTAAATTGGTAGAGGTTGTGGCGTGGGCGGCCAGCAACGGGGATCGTTCAGAAAACGCGGATTATCAGTATGGCAAACGT
CGTTTGCGTGAAATCGACCGTCGTGTGCATTTTTTGACCAAACGAATCGAAGATGCTGAGCTCGTGGATCCAAAACAAAT
GAAAGGCACGACCGTGCTTTTCAGTGCCACAGTCACACTGGTTAATGAAGACGGTGATGAAGTGGTGTATCAGATCGTCG
GCGAAGATGAGTTTGATCCGAAAGCGGGCAAGATTTCCTGGAAGTCTCCTGTGGCGCGTGCGCTTCTTGGAAAAAAGTTG
GGAGATGAGGTGCGAATCATCAAACCTGCAGGCGAAGAGTTCGTTACTATCGAGAACGTAGAGTTTAAGTAA

Upstream 100 bases:

>100_bases
ATAAGCAGACGTTTCCACTGTTTTTTCATCGATTCCTCCTCATGCTTTTTTCGGAGTAAATGTCTCACTATTAAAGGGAG
CTAAAGTCTAGGTCCGCCGT

Downstream 100 bases:

>100_bases
ATTTTACAGTTGTCTTTGTCTGTACATTTTTGCACACTCCTGCGCATAGCAGGGGGAGCTATGAAGTTCATCACTAAAGT
CAGTATTTATCTTTTCGCCT

Product: hypothetical protein

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MGDGIMESKSNSRWTGLEDGLESCGMDNNKNYITPEGLAKLKAEYHALMHVERPKLVEVVAWAASNGDRSENADYQYGKR
RLREIDRRVHFLTKRIEDAELVDPKQMKGTTVLFSATVTLVNEDGDEVVYQIVGEDEFDPKAGKISWKSPVARALLGKKL
GDEVRIIKPAGEEFVTIENVEFK

Sequences:

>Translated_183_residues
MGDGIMESKSNSRWTGLEDGLESCGMDNNKNYITPEGLAKLKAEYHALMHVERPKLVEVVAWAASNGDRSENADYQYGKR
RLREIDRRVHFLTKRIEDAELVDPKQMKGTTVLFSATVTLVNEDGDEVVYQIVGEDEFDPKAGKISWKSPVARALLGKKL
GDEVRIIKPAGEEFVTIENVEFK
>Mature_182_residues
GDGIMESKSNSRWTGLEDGLESCGMDNNKNYITPEGLAKLKAEYHALMHVERPKLVEVVAWAASNGDRSENADYQYGKRR
LREIDRRVHFLTKRIEDAELVDPKQMKGTTVLFSATVTLVNEDGDEVVYQIVGEDEFDPKAGKISWKSPVARALLGKKLG
DEVRIIKPAGEEFVTIENVEFK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=150, Percent_Identity=48, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=36.8421052631579, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20588; Mature: 20456

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDGIMESKSNSRWTGLEDGLESCGMDNNKNYITPEGLAKLKAEYHALMHVERPKLVEVV
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCHHHHHH
AWAASNGDRSENADYQYGKRRLREIDRRVHFLTKRIEDAELVDPKQMKGTTVLFSATVTL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEEEEE
VNEDGDEVVYQIVGEDEFDPKAGKISWKSPVARALLGKKLGDEVRIIKPAGEEFVTIENV
ECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECC
EFK
CCC
>Mature Secondary Structure 
GDGIMESKSNSRWTGLEDGLESCGMDNNKNYITPEGLAKLKAEYHALMHVERPKLVEVV
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCHHHHHH
AWAASNGDRSENADYQYGKRRLREIDRRVHFLTKRIEDAELVDPKQMKGTTVLFSATVTL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEEEEE
VNEDGDEVVYQIVGEDEFDPKAGKISWKSPVARALLGKKLGDEVRIIKPAGEEFVTIENV
ECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECC
EFK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]