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The map label for this gene is rsmF [H]

Identifier: 42522579

GI number: 42522579

Start: 967408

End: 968298

Strand: Direct

Name: rsmF [H]

Synonym: Bd1019

Alternate gene names: 42522579

Gene position: 967408-968298 (Clockwise)

Preceding gene: 42522578

Following gene: 42522581

Centisome position: 25.57

GC content: 54.99

Gene sequence:

>891_bases
ATGAACACCGATCATCCCTTTTATCGTCATTTTGAAAAAGTCTATGGTTCCCGCTGGCCGGGCCTCTTCGCTGCCCTGCA
AACCCGTGAACAACAAGTTGCCCGGGTGAACGCGTGGAGCCCATCCGATAAAAGCACTAAATCCTGGTCTCAGTTCCCTG
AGAAGTCCGAGCTCCCTGGCTGTCATTGGTTGACGCCGGCTCATGGCTGTCAGCCCGAGCGCAATTCGGATGAACTGCTG
GATATCTATATTATGGATCCGGCCAGCGTCATGGTGGCGCGGGCCTTGGACGTGCAGCCGGGTGATCGTCTTTTGGATAT
GTGCGCGGCCCCGGGTGGGAAAAGTCTGGTGATGATTGAATCTTTCGGGGGCGAGGGTGAGATTTTCTGCAATGACCTTT
CGCCGGAGCGTCGCGAACGCTTGAAGAAAGTCATTCAGCAGTATGTGCCGCGCGAAGTGCGCAATCGGGTGTGGGTGACT
GGCAAAGACGGGGTGCAGTTTGGTTTGAAAGAGCCCGGCAGCTTTGACCGGGTTCTGCTGGATGCCCCATGTTCAGGTGA
GCGCCATATTCTGGAAAATCAGGCAGCCCAAGACGAGTGGAGCCCCCGTCGCACCGAACATCTGGCGGCACGGCAGTATT
CATTGCTGGCGGCAGCCTTTCTGGCGGTGAAAGTCGGGGGGCGCATCGTTTATTCCACGTGCTCGATCAGCCCGGCAGAA
AATGACGATGTCGTTCGCAAACTTTTGAAAAAGAAAAAATCAGCGGTGAAGTTGCTGGAAGCACCTGTGGGTGTGGGCGG
AGAGAGAACCGAACTGGGTGTGGCTTACATGCCCGATCAGTGTGGTTTTGGTCCTTTGTATTTTGCTGTGATCGAGAAAG
TCGAAGAATAA

Upstream 100 bases:

>100_bases
ATTATGCAAAGTCACGGTGGTACGGTCGCGGTGAAGAGTATTGTCGACCAGGGATCTGAATTTATTTGCTCTTTCCCGAT
CCGAAAGTAAAATTTGGGGG

Downstream 100 bases:

>100_bases
AAAAGCGAACTTTTCAGTTCGCTTTGTGGGTGACTAGTTGTCGTCTTTTTGACGGAATTTATGTTTGCGGTATTCTTCCA
AAGTTAAAAAACCGTAGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNTDHPFYRHFEKVYGSRWPGLFAALQTREQQVARVNAWSPSDKSTKSWSQFPEKSELPGCHWLTPAHGCQPERNSDELL
DIYIMDPASVMVARALDVQPGDRLLDMCAAPGGKSLVMIESFGGEGEIFCNDLSPERRERLKKVIQQYVPREVRNRVWVT
GKDGVQFGLKEPGSFDRVLLDAPCSGERHILENQAAQDEWSPRRTEHLAARQYSLLAAAFLAVKVGGRIVYSTCSISPAE
NDDVVRKLLKKKKSAVKLLEAPVGVGGERTELGVAYMPDQCGFGPLYFAVIEKVEE

Sequences:

>Translated_296_residues
MNTDHPFYRHFEKVYGSRWPGLFAALQTREQQVARVNAWSPSDKSTKSWSQFPEKSELPGCHWLTPAHGCQPERNSDELL
DIYIMDPASVMVARALDVQPGDRLLDMCAAPGGKSLVMIESFGGEGEIFCNDLSPERRERLKKVIQQYVPREVRNRVWVT
GKDGVQFGLKEPGSFDRVLLDAPCSGERHILENQAAQDEWSPRRTEHLAARQYSLLAAAFLAVKVGGRIVYSTCSISPAE
NDDVVRKLLKKKKSAVKLLEAPVGVGGERTELGVAYMPDQCGFGPLYFAVIEKVEE
>Mature_296_residues
MNTDHPFYRHFEKVYGSRWPGLFAALQTREQQVARVNAWSPSDKSTKSWSQFPEKSELPGCHWLTPAHGCQPERNSDELL
DIYIMDPASVMVARALDVQPGDRLLDMCAAPGGKSLVMIESFGGEGEIFCNDLSPERRERLKKVIQQYVPREVRNRVWVT
GKDGVQFGLKEPGSFDRVLLDAPCSGERHILENQAAQDEWSPRRTEHLAARQYSLLAAAFLAVKVGGRIVYSTCSISPAE
NDDVVRKLLKKKKSAVKLLEAPVGVGGERTELGVAYMPDQCGFGPLYFAVIEKVEE

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI40316918, Length=329, Percent_Identity=31.6109422492401, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI11545785, Length=228, Percent_Identity=33.7719298245614, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI76150625, Length=194, Percent_Identity=40.7216494845361, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI76150623, Length=194, Percent_Identity=40.7216494845361, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI32698918, Length=173, Percent_Identity=32.9479768786127, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI39995082, Length=196, Percent_Identity=29.0816326530612, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI301336155, Length=196, Percent_Identity=29.0816326530612, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI87081985, Length=185, Percent_Identity=36.2162162162162, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI2367212, Length=266, Percent_Identity=26.6917293233083, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509941, Length=240, Percent_Identity=30, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=183, Percent_Identity=34.4262295081967, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25143471, Length=185, Percent_Identity=31.8918918918919, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=191, Percent_Identity=35.0785340314136, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319447, Length=217, Percent_Identity=29.9539170506912, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920494, Length=246, Percent_Identity=31.7073170731707, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI21355201, Length=198, Percent_Identity=31.3131313131313, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI22024126, Length=186, Percent_Identity=35.4838709677419, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24668781, Length=183, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356513, Length=183, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 33108; Mature: 33108

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDHPFYRHFEKVYGSRWPGLFAALQTREQQVARVNAWSPSDKSTKSWSQFPEKSELPG
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCC
CHWLTPAHGCQPERNSDELLDIYIMDPASVMVARALDVQPGDRLLDMCAAPGGKSLVMIE
CEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
SFGGEGEIFCNDLSPERRERLKKVIQQYVPREVRNRVWVTGKDGVQFGLKEPGSFDRVLL
ECCCCCCEEECCCCHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCEECCCCCCCCCEEEE
DAPCSGERHILENQAAQDEWSPRRTEHLAARQYSLLAAAFLAVKVGGRIVYSTCSISPAE
ECCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
NDDVVRKLLKKKKSAVKLLEAPVGVGGERTELGVAYMPDQCGFGPLYFAVIEKVEE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNTDHPFYRHFEKVYGSRWPGLFAALQTREQQVARVNAWSPSDKSTKSWSQFPEKSELPG
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCC
CHWLTPAHGCQPERNSDELLDIYIMDPASVMVARALDVQPGDRLLDMCAAPGGKSLVMIE
CEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
SFGGEGEIFCNDLSPERRERLKKVIQQYVPREVRNRVWVTGKDGVQFGLKEPGSFDRVLL
ECCCCCCEEECCCCHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCEECCCCCCCCCEEEE
DAPCSGERHILENQAAQDEWSPRRTEHLAARQYSLLAAAFLAVKVGGRIVYSTCSISPAE
ECCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
NDDVVRKLLKKKKSAVKLLEAPVGVGGERTELGVAYMPDQCGFGPLYFAVIEKVEE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA