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The map label for this gene is phoB [H]

Identifier: 42522577

GI number: 42522577

Start: 965290

End: 965967

Strand: Direct

Name: phoB [H]

Synonym: Bd1017

Alternate gene names: 42522577

Gene position: 965290-965967 (Clockwise)

Preceding gene: 42522576

Following gene: 42522578

Centisome position: 25.52

GC content: 52.06

Gene sequence:

>678_bases
GTGGTGGAAGATGAGCAGGAAATCCGGGAGCTGATGGCTCTGCACCTGCTTCGTCAAGGGTACCGAGTGACCGAATGTGC
TTCCGCCGAAGAAGCCCTGAACGAAATGAATCGCCAGAACTATTCCTTGTTCGTCCTTGATTGGATGCTGCCGGGCTTGA
GTGGCGTCGACATCGTAGATAAAATCAAAGCCAAAAACACCGGTGCCTCAGTACTGATGGTCACCGCTAAAACCGAACCT
CAGGACATCGTTGCCGGCCTTGAAAAAGGGGCCGACGACTACATGACCAAACCTTTCAATCCTTCCGTTTTCATTGCTCG
AATCAAAGCTCTTTTGCGCCGTTCCCAGGTGCAGGCGGCGGCTCCGGCCGCGGATGATGGCGAAGTGTCCTTGTTGGGTC
TTAAAATCAATTTTAAGTCTTACGAGATCTCCTATAACGGCGAACCTCTGCATTTGACTCCGTCCGAGTTCAAGCTGTTG
GGGGCCCTGGTGCAGAATCATGGCTGCGTTTTGACCCGCGAACAACTGATTGAAAACATTCAGGGCGAGGGTATTAATGT
GGTTGGCCGCACTATCGACACCCACGTCTTCGGATTACGCAAGAAGTTAGGGGAGTGGGGAGACCGTATTGAGACTATCC
GTGGCGTTGGATATCGGGTTAAAGTAGACATCGCATGA

Upstream 100 bases:

>100_bases
GATCACGCGACGAATATCGCAGAAGACGTGATCTTTGCCTTTACCGGCAAGGACGTCCGACACGGAGGAAAGTTTGGCTG
ATAATTCAGTTCATGTGCTA

Downstream 100 bases:

>100_bases
AGCGATTTATCCGATTCCCATGGCGAGTTTACTGGCGCTACTTCTCTTGGCAGGTAGTGGCCTTCAATGGTCTGTATCTG
GCGGTCATTTCTGTTATCGA

Product: DNA-binding response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MVEDEQEIRELMALHLLRQGYRVTECASAEEALNEMNRQNYSLFVLDWMLPGLSGVDIVDKIKAKNTGASVLMVTAKTEP
QDIVAGLEKGADDYMTKPFNPSVFIARIKALLRRSQVQAAAPAADDGEVSLLGLKINFKSYEISYNGEPLHLTPSEFKLL
GALVQNHGCVLTREQLIENIQGEGINVVGRTIDTHVFGLRKKLGEWGDRIETIRGVGYRVKVDIA

Sequences:

>Translated_225_residues
MVEDEQEIRELMALHLLRQGYRVTECASAEEALNEMNRQNYSLFVLDWMLPGLSGVDIVDKIKAKNTGASVLMVTAKTEP
QDIVAGLEKGADDYMTKPFNPSVFIARIKALLRRSQVQAAAPAADDGEVSLLGLKINFKSYEISYNGEPLHLTPSEFKLL
GALVQNHGCVLTREQLIENIQGEGINVVGRTIDTHVFGLRKKLGEWGDRIETIRGVGYRVKVDIA
>Mature_225_residues
MVEDEQEIRELMALHLLRQGYRVTECASAEEALNEMNRQNYSLFVLDWMLPGLSGVDIVDKIKAKNTGASVLMVTAKTEP
QDIVAGLEKGADDYMTKPFNPSVFIARIKALLRRSQVQAAAPAADDGEVSLLGLKINFKSYEISYNGEPLHLTPSEFKLL
GALVQNHGCVLTREQLIENIQGEGINVVGRTIDTHVFGLRKKLGEWGDRIETIRGVGYRVKVDIA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=39.9103139013453, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1789809, Length=222, Percent_Identity=35.5855855855856, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=35.8407079646018, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=34.2222222222222, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI2367329, Length=223, Percent_Identity=34.9775784753363, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790552, Length=218, Percent_Identity=34.4036697247706, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=31.2775330396476, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=32.4324324324324, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=32.5688073394495, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=31.25, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=31.2217194570136, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=29.0909090909091, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=29.004329004329, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI145693140, Length=229, Percent_Identity=30.5676855895196, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24955; Mature: 24955

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDEQEIRELMALHLLRQGYRVTECASAEEALNEMNRQNYSLFVLDWMLPGLSGVDIVD
CCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
KIKAKNTGASVLMVTAKTEPQDIVAGLEKGADDYMTKPFNPSVFIARIKALLRRSQVQAA
HHHCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
APAADDGEVSLLGLKINFKSYEISYNGEPLHLTPSEFKLLGALVQNHGCVLTREQLIENI
CCCCCCCCEEEEEEEEEEEEEEEEECCCEEEECHHHHHHHHHHHHCCCCEEEHHHHHHHH
QGEGINVVGRTIDTHVFGLRKKLGEWGDRIETIRGVGYRVKVDIA
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MVEDEQEIRELMALHLLRQGYRVTECASAEEALNEMNRQNYSLFVLDWMLPGLSGVDIVD
CCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
KIKAKNTGASVLMVTAKTEPQDIVAGLEKGADDYMTKPFNPSVFIARIKALLRRSQVQAA
HHHCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
APAADDGEVSLLGLKINFKSYEISYNGEPLHLTPSEFKLLGALVQNHGCVLTREQLIENI
CCCCCCCCEEEEEEEEEEEEEEEEECCCEEEECHHHHHHHHHHHHCCCCEEEHHHHHHHH
QGEGINVVGRTIDTHVFGLRKKLGEWGDRIETIRGVGYRVKVDIA
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]