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The map label for this gene is rpoN [H]

Identifier: 42522415

GI number: 42522415

Start: 790392

End: 791828

Strand: Direct

Name: rpoN [H]

Synonym: Bd0843

Alternate gene names: 42522415

Gene position: 790392-791828 (Clockwise)

Preceding gene: 42522414

Following gene: 42522416

Centisome position: 20.89

GC content: 48.99

Gene sequence:

>1437_bases
ATGGCTCTTCGACAGACGATGAACCTGAGCCAGTCTCTGGTAATCACTCCACAGCTGCAACAGGCGATCAAGCTTTTGCA
GATGTCGCGTATGGAGTTGGAGTCGGCTGTGCGCTCCGAGCTGGAAGAAAATCCTATTCTGGAAGAAGCCGAATCCGCGC
TGAAAGAAGAAGACCTTCAAAGAACCAAAGAGGCTGCCAACGAAGTTGAGCATTCCGAAACTCCGGACCAGCAGAACATT
CAGGATCCGCAAAAGCAGGATGAATTCGAGTGGGAATCCTACATCGAAGCCAACCAAAAGCCTCCACAGTCCGGCATGGC
GGGTTCTGAAGAGATCATGAACTATGAGAACGTCATCACGGCGTCTCAAACCCTGCACGATCACCTTTACTGGCAAGTTA
AAATGAACGGCTTCTCTGAAGAAGAGGAAAGAGCCGCAGACGCCCTGATCGGCGCCATTGATGATGACGGGTATATCAAA
GTTCCTCTGGAGCAGATCGCTGAAGAGGAAAAAGTGGATCTGGCCCTGCTGGAAGACACTCTGACCCTGATTCACGAATT
TGATCCCCCGGGTGTGGGTGCCCGCGATCTGCAGGAATGTCTGCTGATCCAGGCCAAACACATGGAAGAGGACACCCACG
ATCTGGTCACGCTGATCAAAAATCATGTGAAGGATCTGGAAAAGAAAAACTACGAGGCCATCGCCAAAGCCCTGAACCGC
GACGTTGAAGACATCGTGGAAATCTGCAAAATCATCTATGCGATGGATCCAAAGCCGGGTCGTGCGTTTGTCAGCAGTGA
CACGCACTATGTGACTCCGGATGTTTACGTTTACAAAGTGGGTGATGATTACGTGGTGTCTTTGAATGAAGACGGTTTGC
CGCGTTTGAAAATCTCCAACTTCTATAAAAACATGCTTAAAGGTGGCAAAACCACCGGCGACAAGACCCAGGATTATATC
CAGGAAAAACTTCGTTCTGCCGTTTGGTTGATCAAGTCCATTCACCAAAGACAGCGCACGATCTACAAGGTGACTGAGTC
GATCGTAAAACACCAGCGCGAGTTCTTTGAAAAAGGCTCTGAGCACTTAAAACCGATGGTTCTGCGTGATATCGCCAATG
ATATCGGCATGCACGAATCCACGGTCAGCCGTGTGACCACGGCGAAATACGTGCACACACCACAGGGTATCTACGAACTG
AAATACTTCTTCAATTCCGGTATCAGCTCTTCGGATGGTGATTCACTGGCTTCCGAGTCAGTTAAAATCAAAATCAAGGA
TCTGGTGGCCAAGGAAGATCCGAAAAATCCATTGTCCGATCAAAAGATCGTGGATTTGCTGAAGGTCGAAGGTATTCAGA
TCGCACGTCGTACGGTGGCCAAATACCGCGACGTTCTTAAGATTCTTCCTTCTTCTCAACGTAAGAAGTACTTCTAG

Upstream 100 bases:

>100_bases
AATCGCAAATTCCGAATTGGCACGTAAGTTCTATCTCGGCGAAAACTTTAAGCTGTAAAGCCATCCCGGCTGTTACGGTT
CTTGGGAAAGGGAAATTTTA

Downstream 100 bases:

>100_bases
TCTTTTGCGAAGAACGGCTTGGGATGAGCTATCCCGGGCCGTCTGCTTCGGACAGTCCTCGCTCGTTTTTCCCCTTCTTG
TTTCTCGTCGTTTTATTCCT

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MALRQTMNLSQSLVITPQLQQAIKLLQMSRMELESAVRSELEENPILEEAESALKEEDLQRTKEAANEVEHSETPDQQNI
QDPQKQDEFEWESYIEANQKPPQSGMAGSEEIMNYENVITASQTLHDHLYWQVKMNGFSEEEERAADALIGAIDDDGYIK
VPLEQIAEEEKVDLALLEDTLTLIHEFDPPGVGARDLQECLLIQAKHMEEDTHDLVTLIKNHVKDLEKKNYEAIAKALNR
DVEDIVEICKIIYAMDPKPGRAFVSSDTHYVTPDVYVYKVGDDYVVSLNEDGLPRLKISNFYKNMLKGGKTTGDKTQDYI
QEKLRSAVWLIKSIHQRQRTIYKVTESIVKHQREFFEKGSEHLKPMVLRDIANDIGMHESTVSRVTTAKYVHTPQGIYEL
KYFFNSGISSSDGDSLASESVKIKIKDLVAKEDPKNPLSDQKIVDLLKVEGIQIARRTVAKYRDVLKILPSSQRKKYF

Sequences:

>Translated_478_residues
MALRQTMNLSQSLVITPQLQQAIKLLQMSRMELESAVRSELEENPILEEAESALKEEDLQRTKEAANEVEHSETPDQQNI
QDPQKQDEFEWESYIEANQKPPQSGMAGSEEIMNYENVITASQTLHDHLYWQVKMNGFSEEEERAADALIGAIDDDGYIK
VPLEQIAEEEKVDLALLEDTLTLIHEFDPPGVGARDLQECLLIQAKHMEEDTHDLVTLIKNHVKDLEKKNYEAIAKALNR
DVEDIVEICKIIYAMDPKPGRAFVSSDTHYVTPDVYVYKVGDDYVVSLNEDGLPRLKISNFYKNMLKGGKTTGDKTQDYI
QEKLRSAVWLIKSIHQRQRTIYKVTESIVKHQREFFEKGSEHLKPMVLRDIANDIGMHESTVSRVTTAKYVHTPQGIYEL
KYFFNSGISSSDGDSLASESVKIKIKDLVAKEDPKNPLSDQKIVDLLKVEGIQIARRTVAKYRDVLKILPSSQRKKYF
>Mature_477_residues
ALRQTMNLSQSLVITPQLQQAIKLLQMSRMELESAVRSELEENPILEEAESALKEEDLQRTKEAANEVEHSETPDQQNIQ
DPQKQDEFEWESYIEANQKPPQSGMAGSEEIMNYENVITASQTLHDHLYWQVKMNGFSEEEERAADALIGAIDDDGYIKV
PLEQIAEEEKVDLALLEDTLTLIHEFDPPGVGARDLQECLLIQAKHMEEDTHDLVTLIKNHVKDLEKKNYEAIAKALNRD
VEDIVEICKIIYAMDPKPGRAFVSSDTHYVTPDVYVYKVGDDYVVSLNEDGLPRLKISNFYKNMLKGGKTTGDKTQDYIQ
EKLRSAVWLIKSIHQRQRTIYKVTESIVKHQREFFEKGSEHLKPMVLRDIANDIGMHESTVSRVTTAKYVHTPQGIYELK
YFFNSGISSSDGDSLASESVKIKIKDLVAKEDPKNPLSDQKIVDLLKVEGIQIARRTVAKYRDVLKILPSSQRKKYF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=481, Percent_Identity=39.5010395010395, Blast_Score=355, Evalue=2e-99,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54628; Mature: 54497

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRQTMNLSQSLVITPQLQQAIKLLQMSRMELESAVRSELEENPILEEAESALKEEDLQ
CCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RTKEAANEVEHSETPDQQNIQDPQKQDEFEWESYIEANQKPPQSGMAGSEEIMNYENVIT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ASQTLHDHLYWQVKMNGFSEEEERAADALIGAIDDDGYIKVPLEQIAEEEKVDLALLEDT
HHHHHHHHEEEEEEECCCCCHHHHHHHHHHEEECCCCEEECCHHHHCCHHHHHHHHHHHH
LTLIHEFDPPGVGARDLQECLLIQAKHMEEDTHDLVTLIKNHVKDLEKKNYEAIAKALNR
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
DVEDIVEICKIIYAMDPKPGRAFVSSDTHYVTPDVYVYKVGDDYVVSLNEDGLPRLKISN
CHHHHHHHHHHHHHCCCCCCCEEECCCCEEECCCEEEEEECCCEEEEECCCCCCCHHHHH
FYKNMLKGGKTTGDKTQDYIQEKLRSAVWLIKSIHQRQRTIYKVTESIVKHQREFFEKGS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EHLKPMVLRDIANDIGMHESTVSRVTTAKYVHTPQGIYELKYFFNSGISSSDGDSLASES
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHCCC
VKIKIKDLVAKEDPKNPLSDQKIVDLLKVEGIQIARRTVAKYRDVLKILPSSQRKKYF
CEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ALRQTMNLSQSLVITPQLQQAIKLLQMSRMELESAVRSELEENPILEEAESALKEEDLQ
CCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RTKEAANEVEHSETPDQQNIQDPQKQDEFEWESYIEANQKPPQSGMAGSEEIMNYENVIT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ASQTLHDHLYWQVKMNGFSEEEERAADALIGAIDDDGYIKVPLEQIAEEEKVDLALLEDT
HHHHHHHHEEEEEEECCCCCHHHHHHHHHHEEECCCCEEECCHHHHCCHHHHHHHHHHHH
LTLIHEFDPPGVGARDLQECLLIQAKHMEEDTHDLVTLIKNHVKDLEKKNYEAIAKALNR
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
DVEDIVEICKIIYAMDPKPGRAFVSSDTHYVTPDVYVYKVGDDYVVSLNEDGLPRLKISN
CHHHHHHHHHHHHHCCCCCCCEEECCCCEEECCCEEEEEECCCEEEEECCCCCCCHHHHH
FYKNMLKGGKTTGDKTQDYIQEKLRSAVWLIKSIHQRQRTIYKVTESIVKHQREFFEKGS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EHLKPMVLRDIANDIGMHESTVSRVTTAKYVHTPQGIYELKYFFNSGISSSDGDSLASES
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHCCC
VKIKIKDLVAKEDPKNPLSDQKIVDLLKVEGIQIARRTVAKYRDVLKILPSSQRKKYF
CEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]