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The map label for this gene is polA [H]

Identifier: 42522378

GI number: 42522378

Start: 750574

End: 753165

Strand: Direct

Name: polA [H]

Synonym: Bd0802

Alternate gene names: 42522378

Gene position: 750574-753165 (Clockwise)

Preceding gene: 42522377

Following gene: 42522380

Centisome position: 19.84

GC content: 51.85

Gene sequence:

>2592_bases
ATGAAGAAAATCTACCTTATTGACGTCAGTGCCATGTTCTTTCGCGCCTTCTACGCGATTCGTGCCCTTTCGTCTCCAAG
CGGAGTTCCGGTTAACGCCGTTTACGGCTTCCTTTCCATGATGATCAAACTTCTGAAAGAGGAAAAACCCGAGTATCTGG
TTTTCTGCTATGACCGCAAAGAACCCTCTTTCCGAAAAACCATGTACGATGGCTACAAAGCTCACCGTACCGAGATGCCG
GACGAACTGGCCCAACAAATCCCTTATATCAAAAGACTGGCAGACCTGCTGGGAGTTCCCGCATTTGAAGTCGAATCCTT
CGAGGCCGATGACATCATCGGGACATTGACTAAATGGAGCCGTCGCAATGGCATGGAAGTGTTCATTGTCAGCGGCGATA
AAGACTTCGGCCAGCTGATCGAAGACGGCGTCACGCTGTACGACACGATGAAGGGTGTTCGCTACAACGCTGAAGGTGTC
TTTGAAAAGTGGGGTGTGCGCCCGGATCAGTTTATCGACTATCTGGCGATTGTGGGGGATGCTTCGGACAACGTTCCGGG
CGTAAAGGGTGTCGGGGAAAAAGGCGCCATCAAGCTTTTGGAGCAGTTCAAACACGTCGAAGACATCTATGAAAATATCG
ACAAAGTTGAAAGCAAAAGCGTTCGTGAAAAGCTGATTGCCTCCAAAGACAATGCACTACTGTCCAAAAAGCTGGTGACT
ATTTCCTGCGACGTACCGATGCCGGATGACCTTGAGGCCTACAAACTCAAGCCGTTCAAGGCGGACGAACTGCGTGCCTT
CCTGCATGAGCTGAATTTTAAATCCTTCGAAAAAAGTCTGTTTGGAACAGTGGACACGACTCCGGCAGCGGCGGCACCAG
CGGCTCCGGCCGGTCCGTTGATCAAGACGGCAGCCCCCTCCGCGCCATTGAGTATTGCCGATGATGTCGCGCCGACCATC
GTGATTGCCAAAGAAGAGCGTCAGTTCACTGAAAAAAGCATGACAACCCGCGAGCTGGCCGACTTCCTGGCGGAACACCA
AAGCCTTTGGGGCTTTAGCGACATGCGTGGAGTCTTCATCGGAACTGAGTCCCAGATCATCCAGGTCCCTGATCATGAAT
ATCTGGGGAAGTTGACCGACACTTTCCAAGTTCAATGGCATGGCTATGACCTGAAGTCCTTCTGGCACAAGATCGGAGCG
AAAACTCCGGTGGCGGCCTGGGATTCCCAGTTGGCGGCTTATGTGTTGAAGGCCGGTGACACCTCCGATTTTGCCAAGAT
CTACACTCGCTTTATGGCCGATGTGTTGCCGGATTTGGCATCACCATCGCAGCTGTACAATGCTCAATTGAATTTTGCCA
CAACCCTGAAATCCCAGTTGCAGACCGTGCAGGGTGAAAAGGTTTATCAGGAACTGGATCTTCCATTGGCCCGTGTGCTT
TTGTCCATGGAGCGTCTGGGGGTTCGCATCGACAAGGATCTTTTGGCCAAACAAAGTGAAGAGCTGGCGAAAGACATCGC
AGAAATCGAAAAACAAATCCACGAAGCGGCAGGGGAGTCCTTTAACGTCGGAAGCCCGAAGCAGCTGGGCGTGATCCTGT
TTGAAAAACTGGGCCTGCCTGCCGGGAAAAAAACAAAAACCGGCTATTCCACAGACGAAGAAGTGTTGTTGGGTCTGGAG
CATCCGATTGCAAAACTGGTTTTGCAATGGCGTGAGATGTCCAAGCTGAAGTCCACTTACGTCGATGCCTTGCCAACCAT
GATTGATCCTAAAGACGAGCGCGTGCACACGAGCTTTAATCAGGCCCTGACCACCACGGGCCGTCTTTCCAGCACGCAGC
CGAATTTGCAGAATATTCCAATTCGTACCGCGCGCGGCCAGCAGGTGCGCAAGGCGTTCATCGCGGCTCCGCGCATGAAG
CTTCTGTCGGTGGATTATTCCCAGATTGAACTGCGCATCCTGGCGCATATTTCCGAAGACCCGAACTTGTGCAAAGCCTT
TGCTGAAGACCTCGACATTCACGCGGCCACCGCAGCGGAAGTGTTTGGGGTGCCTTTGAAAGATGTGACTGCTGAACACC
GTCGTTCTGCGAAGGCCGTGAACTTTGGAATTGCTTACGGTCAGGGGGCCTTCGGTCTGGCGGAAAACCTGGGTATTTCA
CGCACCGAAGCCAAGGATATTATCGAGCGCTATTTCAACCGCTTTAAAAACGTCCGCGAGTATATCGATGGCACCATCAA
GATGGCTCACGAAAAAGGCTATGTTGAAACGCTGTTTGGACGCCGCCGTTATATCGACGAGCTTCAGTCCAAGAACATGG
CTTTAAAAAAGTTCGGCGAGCGCGCCGCGATCAACGCCCCGATTCAGGGGACTGCCAGTGATCTGGTGAAAAAGGCGATG
ATCGAAGTGTTTGAAAAAGTGCCGGTGCGCATGCTGTTGCAGGTGCACGATGAATTGATCTTCGAGGATTTCGAGGAGAA
TCTGCAGAAACATTCCCCTCAGTTGGTTTCAATCATGGAAAACGTGATGAAGCTGAAAGTGCCGTTGAAGGTCAATTATG
CGATCGGGAACAACTGGGACGAAGCTCACTAG

Upstream 100 bases:

>100_bases
AATCGATTTCACTCGCGTGAAAGCCGAATTGAAATAGGCATCAATCATCGACAATCAGGCCTGTGCGGTGTAGACCTTTG
GTTTACATCCGGAGGCCTTC

Downstream 100 bases:

>100_bases
GGCTTCGTCTTATTCAGTTTCCCAGCCAACCACGCGTCCGCCTTCAAAATACACATAACGTGTTTCTTTGCGGTAGCCTT
CCGGGGCGGACACAAACTTC

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MKKIYLIDVSAMFFRAFYAIRALSSPSGVPVNAVYGFLSMMIKLLKEEKPEYLVFCYDRKEPSFRKTMYDGYKAHRTEMP
DELAQQIPYIKRLADLLGVPAFEVESFEADDIIGTLTKWSRRNGMEVFIVSGDKDFGQLIEDGVTLYDTMKGVRYNAEGV
FEKWGVRPDQFIDYLAIVGDASDNVPGVKGVGEKGAIKLLEQFKHVEDIYENIDKVESKSVREKLIASKDNALLSKKLVT
ISCDVPMPDDLEAYKLKPFKADELRAFLHELNFKSFEKSLFGTVDTTPAAAAPAAPAGPLIKTAAPSAPLSIADDVAPTI
VIAKEERQFTEKSMTTRELADFLAEHQSLWGFSDMRGVFIGTESQIIQVPDHEYLGKLTDTFQVQWHGYDLKSFWHKIGA
KTPVAAWDSQLAAYVLKAGDTSDFAKIYTRFMADVLPDLASPSQLYNAQLNFATTLKSQLQTVQGEKVYQELDLPLARVL
LSMERLGVRIDKDLLAKQSEELAKDIAEIEKQIHEAAGESFNVGSPKQLGVILFEKLGLPAGKKTKTGYSTDEEVLLGLE
HPIAKLVLQWREMSKLKSTYVDALPTMIDPKDERVHTSFNQALTTTGRLSSTQPNLQNIPIRTARGQQVRKAFIAAPRMK
LLSVDYSQIELRILAHISEDPNLCKAFAEDLDIHAATAAEVFGVPLKDVTAEHRRSAKAVNFGIAYGQGAFGLAENLGIS
RTEAKDIIERYFNRFKNVREYIDGTIKMAHEKGYVETLFGRRRYIDELQSKNMALKKFGERAAINAPIQGTASDLVKKAM
IEVFEKVPVRMLLQVHDELIFEDFEENLQKHSPQLVSIMENVMKLKVPLKVNYAIGNNWDEAH

Sequences:

>Translated_863_residues
MKKIYLIDVSAMFFRAFYAIRALSSPSGVPVNAVYGFLSMMIKLLKEEKPEYLVFCYDRKEPSFRKTMYDGYKAHRTEMP
DELAQQIPYIKRLADLLGVPAFEVESFEADDIIGTLTKWSRRNGMEVFIVSGDKDFGQLIEDGVTLYDTMKGVRYNAEGV
FEKWGVRPDQFIDYLAIVGDASDNVPGVKGVGEKGAIKLLEQFKHVEDIYENIDKVESKSVREKLIASKDNALLSKKLVT
ISCDVPMPDDLEAYKLKPFKADELRAFLHELNFKSFEKSLFGTVDTTPAAAAPAAPAGPLIKTAAPSAPLSIADDVAPTI
VIAKEERQFTEKSMTTRELADFLAEHQSLWGFSDMRGVFIGTESQIIQVPDHEYLGKLTDTFQVQWHGYDLKSFWHKIGA
KTPVAAWDSQLAAYVLKAGDTSDFAKIYTRFMADVLPDLASPSQLYNAQLNFATTLKSQLQTVQGEKVYQELDLPLARVL
LSMERLGVRIDKDLLAKQSEELAKDIAEIEKQIHEAAGESFNVGSPKQLGVILFEKLGLPAGKKTKTGYSTDEEVLLGLE
HPIAKLVLQWREMSKLKSTYVDALPTMIDPKDERVHTSFNQALTTTGRLSSTQPNLQNIPIRTARGQQVRKAFIAAPRMK
LLSVDYSQIELRILAHISEDPNLCKAFAEDLDIHAATAAEVFGVPLKDVTAEHRRSAKAVNFGIAYGQGAFGLAENLGIS
RTEAKDIIERYFNRFKNVREYIDGTIKMAHEKGYVETLFGRRRYIDELQSKNMALKKFGERAAINAPIQGTASDLVKKAM
IEVFEKVPVRMLLQVHDELIFEDFEENLQKHSPQLVSIMENVMKLKVPLKVNYAIGNNWDEAH
>Mature_863_residues
MKKIYLIDVSAMFFRAFYAIRALSSPSGVPVNAVYGFLSMMIKLLKEEKPEYLVFCYDRKEPSFRKTMYDGYKAHRTEMP
DELAQQIPYIKRLADLLGVPAFEVESFEADDIIGTLTKWSRRNGMEVFIVSGDKDFGQLIEDGVTLYDTMKGVRYNAEGV
FEKWGVRPDQFIDYLAIVGDASDNVPGVKGVGEKGAIKLLEQFKHVEDIYENIDKVESKSVREKLIASKDNALLSKKLVT
ISCDVPMPDDLEAYKLKPFKADELRAFLHELNFKSFEKSLFGTVDTTPAAAAPAAPAGPLIKTAAPSAPLSIADDVAPTI
VIAKEERQFTEKSMTTRELADFLAEHQSLWGFSDMRGVFIGTESQIIQVPDHEYLGKLTDTFQVQWHGYDLKSFWHKIGA
KTPVAAWDSQLAAYVLKAGDTSDFAKIYTRFMADVLPDLASPSQLYNAQLNFATTLKSQLQTVQGEKVYQELDLPLARVL
LSMERLGVRIDKDLLAKQSEELAKDIAEIEKQIHEAAGESFNVGSPKQLGVILFEKLGLPAGKKTKTGYSTDEEVLLGLE
HPIAKLVLQWREMSKLKSTYVDALPTMIDPKDERVHTSFNQALTTTGRLSSTQPNLQNIPIRTARGQQVRKAFIAAPRMK
LLSVDYSQIELRILAHISEDPNLCKAFAEDLDIHAATAAEVFGVPLKDVTAEHRRSAKAVNFGIAYGQGAFGLAENLGIS
RTEAKDIIERYFNRFKNVREYIDGTIKMAHEKGYVETLFGRRRYIDELQSKNMALKKFGERAAINAPIQGTASDLVKKAM
IEVFEKVPVRMLLQVHDELIFEDFEENLQKHSPQLVSIMENVMKLKVPLKVNYAIGNNWDEAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=433, Percent_Identity=33.4872979214781, Blast_Score=231, Evalue=3e-60,
Organism=Homo sapiens, GI139394648, Length=532, Percent_Identity=26.8796992481203, Blast_Score=187, Evalue=5e-47,
Organism=Escherichia coli, GI1790294, Length=922, Percent_Identity=39.6963123644252, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI226510970, Length=250, Percent_Identity=30.4, Blast_Score=122, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI86563272, Length=507, Percent_Identity=26.232741617357, Blast_Score=169, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17933644, Length=415, Percent_Identity=33.7349397590361, Blast_Score=222, Evalue=7e-58,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 96887; Mature: 96887

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYLIDVSAMFFRAFYAIRALSSPSGVPVNAVYGFLSMMIKLLKEEKPEYLVFCYDRK
CCEEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
EPSFRKTMYDGYKAHRTEMPDELAQQIPYIKRLADLLGVPAFEVESFEADDIIGTLTKWS
CCHHHHHHHHCHHHHHCCCCHHHHHHCHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHH
RRNGMEVFIVSGDKDFGQLIEDGVTLYDTMKGVRYNAEGVFEKWGVRPDQFIDYLAIVGD
CCCCCEEEEEECCCHHHHHHHCCCHHHHHHHCCEECCCHHHHHHCCCHHHHHHHHHHCCC
ASDNVPGVKGVGEKGAIKLLEQFKHVEDIYENIDKVESKSVREKLIASKDNALLSKKLVT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEE
ISCDVPMPDDLEAYKLKPFKADELRAFLHELNFKSFEKSLFGTVDTTPAAAAPAAPAGPL
EECCCCCCCCCCHHCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
IKTAAPSAPLSIADDVAPTIVIAKEERQFTEKSMTTRELADFLAEHQSLWGFSDMRGVFI
CCCCCCCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GTESQIIQVPDHEYLGKLTDTFQVQWHGYDLKSFWHKIGAKTPVAAWDSQLAAYVLKAGD
ECCCCEEECCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TSDFAKIYTRFMADVLPDLASPSQLYNAQLNFATTLKSQLQTVQGEKVYQELDLPLARVL
CHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LSMERLGVRIDKDLLAKQSEELAKDIAEIEKQIHEAAGESFNVGSPKQLGVILFEKLGLP
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
AGKKTKTGYSTDEEVLLGLEHPIAKLVLQWREMSKLKSTYVDALPTMIDPKDERVHTSFN
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QALTTTGRLSSTQPNLQNIPIRTARGQQVRKAFIAAPRMKLLSVDYSQIELRILAHISED
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHEEEECCCC
PNLCKAFAEDLDIHAATAAEVFGVPLKDVTAEHRRSAKAVNFGIAYGQGAFGLAENLGIS
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCC
RTEAKDIIERYFNRFKNVREYIDGTIKMAHEKGYVETLFGRRRYIDELQSKNMALKKFGE
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCC
RAAINAPIQGTASDLVKKAMIEVFEKVPVRMLLQVHDELIFEDFEENLQKHSPQLVSIME
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NVMKLKVPLKVNYAIGNNWDEAH
HHHHEECCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKKIYLIDVSAMFFRAFYAIRALSSPSGVPVNAVYGFLSMMIKLLKEEKPEYLVFCYDRK
CCEEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
EPSFRKTMYDGYKAHRTEMPDELAQQIPYIKRLADLLGVPAFEVESFEADDIIGTLTKWS
CCHHHHHHHHCHHHHHCCCCHHHHHHCHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHH
RRNGMEVFIVSGDKDFGQLIEDGVTLYDTMKGVRYNAEGVFEKWGVRPDQFIDYLAIVGD
CCCCCEEEEEECCCHHHHHHHCCCHHHHHHHCCEECCCHHHHHHCCCHHHHHHHHHHCCC
ASDNVPGVKGVGEKGAIKLLEQFKHVEDIYENIDKVESKSVREKLIASKDNALLSKKLVT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEE
ISCDVPMPDDLEAYKLKPFKADELRAFLHELNFKSFEKSLFGTVDTTPAAAAPAAPAGPL
EECCCCCCCCCCHHCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
IKTAAPSAPLSIADDVAPTIVIAKEERQFTEKSMTTRELADFLAEHQSLWGFSDMRGVFI
CCCCCCCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GTESQIIQVPDHEYLGKLTDTFQVQWHGYDLKSFWHKIGAKTPVAAWDSQLAAYVLKAGD
ECCCCEEECCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TSDFAKIYTRFMADVLPDLASPSQLYNAQLNFATTLKSQLQTVQGEKVYQELDLPLARVL
CHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LSMERLGVRIDKDLLAKQSEELAKDIAEIEKQIHEAAGESFNVGSPKQLGVILFEKLGLP
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
AGKKTKTGYSTDEEVLLGLEHPIAKLVLQWREMSKLKSTYVDALPTMIDPKDERVHTSFN
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QALTTTGRLSSTQPNLQNIPIRTARGQQVRKAFIAAPRMKLLSVDYSQIELRILAHISED
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHEEEECCCC
PNLCKAFAEDLDIHAATAAEVFGVPLKDVTAEHRRSAKAVNFGIAYGQGAFGLAENLGIS
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCC
RTEAKDIIERYFNRFKNVREYIDGTIKMAHEKGYVETLFGRRRYIDELQSKNMALKKFGE
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCC
RAAINAPIQGTASDLVKKAMIEVFEKVPVRMLLQVHDELIFEDFEENLQKHSPQLVSIME
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NVMKLKVPLKVNYAIGNNWDEAH
HHHHEECCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]