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The map label for this gene is yloN

Identifier: 42522317

GI number: 42522317

Start: 693899

End: 695098

Strand: Direct

Name: yloN

Synonym: Bd0733

Alternate gene names: 42522317

Gene position: 693899-695098 (Clockwise)

Preceding gene: 42522312

Following gene: 42522318

Centisome position: 18.34

GC content: 50.25

Gene sequence:

>1200_bases
ATGGAAGCAAACGCCGGCAGCACTCTTGCGCCCGTTAATTATTCTGACGACAACGTCGCCAAACCCCTTGAAAATCAACC
TGTCAATTTCTACTCCCTGACACTGGAAGATCTGAAAGCCTATATCAAAAGCAAAGGCAAAGAGCAGTTCCGTGCCCAGC
AGATTTTCAAATGGGTTTATGAGCAGCGTGTGACGGATCCGGAACAGATGACCAATCTGTCCAAGGAATTCCGTCAGGAT
CTTCCCTCCATGCTTTCTTTCGATCTGCCGCCGGTTCTTCAGCATTTGAAGTCTGTCGATGGCACCCAGAAGTTCCTGTT
CGACATGAAAGACGGTATGTCCGTTGAAGCGGTGGTGATTCCTTCCGAAGACCGCCTGACTTTGTGTATTTCCTCTGAAG
TGGGCTGTAACATGGCCTGCAAATTCTGCTTCACTGGCAAACAAAAACTGAAACGCCGTTTGCGCACAGAAGACATCGTG
GGTCAGTTCATGCAGGTTCATGATCGTCTGGCTGAAGGTCAGCGCATCACCAATATCGTATTCATGGGTATGGGCGAGCC
GCTGGACAATCCGGAAGCAGTCTTCAAAACCATCGACGTGATTCATTCTCCGTGGGGTATCAACCTGTCCCGCAAGAAGA
TCACGGTCTCCACTTCCGGTATCGTGCCGGAAATGTGGCGTGTGGCTGATGCGAAAGTGCGTCTGGCAGTCAGCCTGAAC
GGTCCTAACGATGAAATCCGTTCTCAGGTGATGCCGATCAATAAACGCTGGGATACCAAGGCGTTGCTGGAAGCTTGTAA
AGAGCACTACCGTGTTTCCAAAGACAAGATCACTTTCGAGTACGTGTTGCTGAAAGGGATCACCGATCAGTTGGAGCACG
CCCGTCAGCTGGTGAAACTGGTAAAAGACGTTCCATGCAAAATCAATATCATCCCGTTCAACGAACACCCGGGTTCTGGT
TACGAGCGTCCTGATGACGACACAATCCAGGCATTCCATACGGAATTGATGAACCTGGGCGCACACGTTCTTCTTCGTCG
TTCCATGGGTCGTGACATCTTCGCGGCTTGCGGTCAGTTGACCACGGTGAAAGAGCGTCCTCAGACGATGGATATTTCCA
ACTCTCGTCTGGCGGGTCTTCCTAAATACAAACGTGAACTTTTGGCTGCGCAAGAAGCAGAACAAAACAATCAACACTAG

Upstream 100 bases:

>100_bases
AACCCCGCACACATAATCCTTTAACCAACGGGATCAGCCTTAGTGAAGGTAAGGTCCCAGCTGTAACAAGCACGGCTGTA
AAAAGCCAAGGAGCCCGAAG

Downstream 100 bases:

>100_bases
AGGTAACATTATGTCTGAAATTCTTGTTGTCGGCAGTCTGGCTTACGATTCCATTGAAACTCCATCCGGTAAAGTGGACC
GCGCTTTGGGTGGTTCCGCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MEANAGSTLAPVNYSDDNVAKPLENQPVNFYSLTLEDLKAYIKSKGKEQFRAQQIFKWVYEQRVTDPEQMTNLSKEFRQD
LPSMLSFDLPPVLQHLKSVDGTQKFLFDMKDGMSVEAVVIPSEDRLTLCISSEVGCNMACKFCFTGKQKLKRRLRTEDIV
GQFMQVHDRLAEGQRITNIVFMGMGEPLDNPEAVFKTIDVIHSPWGINLSRKKITVSTSGIVPEMWRVADAKVRLAVSLN
GPNDEIRSQVMPINKRWDTKALLEACKEHYRVSKDKITFEYVLLKGITDQLEHARQLVKLVKDVPCKINIIPFNEHPGSG
YERPDDDTIQAFHTELMNLGAHVLLRRSMGRDIFAACGQLTTVKERPQTMDISNSRLAGLPKYKRELLAAQEAEQNNQH

Sequences:

>Translated_399_residues
MEANAGSTLAPVNYSDDNVAKPLENQPVNFYSLTLEDLKAYIKSKGKEQFRAQQIFKWVYEQRVTDPEQMTNLSKEFRQD
LPSMLSFDLPPVLQHLKSVDGTQKFLFDMKDGMSVEAVVIPSEDRLTLCISSEVGCNMACKFCFTGKQKLKRRLRTEDIV
GQFMQVHDRLAEGQRITNIVFMGMGEPLDNPEAVFKTIDVIHSPWGINLSRKKITVSTSGIVPEMWRVADAKVRLAVSLN
GPNDEIRSQVMPINKRWDTKALLEACKEHYRVSKDKITFEYVLLKGITDQLEHARQLVKLVKDVPCKINIIPFNEHPGSG
YERPDDDTIQAFHTELMNLGAHVLLRRSMGRDIFAACGQLTTVKERPQTMDISNSRLAGLPKYKRELLAAQEAEQNNQH
>Mature_399_residues
MEANAGSTLAPVNYSDDNVAKPLENQPVNFYSLTLEDLKAYIKSKGKEQFRAQQIFKWVYEQRVTDPEQMTNLSKEFRQD
LPSMLSFDLPPVLQHLKSVDGTQKFLFDMKDGMSVEAVVIPSEDRLTLCISSEVGCNMACKFCFTGKQKLKRRLRTEDIV
GQFMQVHDRLAEGQRITNIVFMGMGEPLDNPEAVFKTIDVIHSPWGINLSRKKITVSTSGIVPEMWRVADAKVRLAVSLN
GPNDEIRSQVMPINKRWDTKALLEACKEHYRVSKDKITFEYVLLKGITDQLEHARQLVKLVKDVPCKINIIPFNEHPGSG
YERPDDDTIQAFHTELMNLGAHVLLRRSMGRDIFAACGQLTTVKERPQTMDISNSRLAGLPKYKRELLAAQEAEQNNQH

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=343, Percent_Identity=40.2332361516035, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BDEBA (Q6MPV7)

Other databases:

- EMBL:   BX842647
- RefSeq:   NP_967697.1
- ProteinModelPortal:   Q6MPV7
- GeneID:   2734290
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0733
- NMPDR:   fig|264462.1.peg.667
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q6MPV7
- BioCyc:   BBAC264462:BD0733-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 45370; Mature: 45370

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEANAGSTLAPVNYSDDNVAKPLENQPVNFYSLTLEDLKAYIKSKGKEQFRAQQIFKWVY
CCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHH
EQRVTDPEQMTNLSKEFRQDLPSMLSFDLPPVLQHLKSVDGTQKFLFDMKDGMSVEAVVI
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEE
PSEDRLTLCISSEVGCNMACKFCFTGKQKLKRRLRTEDIVGQFMQVHDRLAEGQRITNIV
CCCCCEEEEECCCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FMGMGEPLDNPEAVFKTIDVIHSPWGINLSRKKITVSTSGIVPEMWRVADAKVRLAVSLN
EEECCCCCCCHHHHHHHHHHHCCCCCCCEECCEEEEECCCCCHHHHHHCCCEEEEEEEEC
GPNDEIRSQVMPINKRWDTKALLEACKEHYRVSKDKITFEYVLLKGITDQLEHARQLVKL
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
VKDVPCKINIIPFNEHPGSGYERPDDDTIQAFHTELMNLGAHVLLRRSMGRDIFAACGQL
HHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCH
TTVKERPQTMDISNSRLAGLPKYKRELLAAQEAEQNNQH
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEANAGSTLAPVNYSDDNVAKPLENQPVNFYSLTLEDLKAYIKSKGKEQFRAQQIFKWVY
CCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHH
EQRVTDPEQMTNLSKEFRQDLPSMLSFDLPPVLQHLKSVDGTQKFLFDMKDGMSVEAVVI
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEE
PSEDRLTLCISSEVGCNMACKFCFTGKQKLKRRLRTEDIVGQFMQVHDRLAEGQRITNIV
CCCCCEEEEECCCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FMGMGEPLDNPEAVFKTIDVIHSPWGINLSRKKITVSTSGIVPEMWRVADAKVRLAVSLN
EEECCCCCCCHHHHHHHHHHHCCCCCCCEECCEEEEECCCCCHHHHHHCCCEEEEEEEEC
GPNDEIRSQVMPINKRWDTKALLEACKEHYRVSKDKITFEYVLLKGITDQLEHARQLVKL
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
VKDVPCKINIIPFNEHPGSGYERPDDDTIQAFHTELMNLGAHVLLRRSMGRDIFAACGQL
HHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCH
TTVKERPQTMDISNSRLAGLPKYKRELLAAQEAEQNNQH
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA