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The map label for this gene is yjeA [H]

Identifier: 42522288

GI number: 42522288

Start: 653220

End: 654437

Strand: Direct

Name: yjeA [H]

Synonym: Bd0702

Alternate gene names: 42522288

Gene position: 653220-654437 (Clockwise)

Preceding gene: 42522287

Following gene: 42522289

Centisome position: 17.27

GC content: 49.59

Gene sequence:

>1218_bases
TTGAGCCGAAATGACTTTTTAACCCAGATTTGGAAGCCCTATCCCGCAATGCCTGAAAATGCTCAGGCGGCCGGGCGTAT
TTATCAGATCGAACGCGAAGGCGACGCTTTAAAGCTGGTCCTGCACCGCGATTCCGAAACTCACAAAATCCATTTTGAAA
AAGCCCCGAAGCACTCTGAATTTTTGATTGAGGGTGACATCGTTGCCGTTGTCTCGGCCTCTGAAGTGGTGCTGCTGGCG
CCGCAAAAGCAATCCCTGCCGAATCGTTCCTATAACAAGGAATTGACTCAAAATTGGGGCACCTATCTGCAATCTTTACG
TGAATTCTTTGTTTCTAAAGGCTTTGTGGAATTGAAGACGCCGTCGCTGGTGGTCTGTCCGGGGACGGAGCCCAGTCTGG
ATGTTTTTTCCACGAACTTGAAGATCGGTTCGCGCCAGCAGAAATTGTTTTTACCCACAAGCCCTGAACTGCATTTGAAA
AAGGCTTTGGCATTGGGGGCAGAAAAAATCTTTGAGCTGGCTCCGTGTTTCCGTAACGGCGAAGTTACTGAAAGACATCA
GCCTGAATTTTTGATGCTGGAGTGGTATCGTGCGTATGCCTCTTTAAAGTTGATTAAAGAAGACACCGTCAAACTGGTCA
GCCATCTGGCGCAAGCGATGAAAGTCGCAGGCCCAGTGAAGGTGACTTCATATTCCGTGGCCGAGCTTTTCAAAATTCAC
TGTGGTTTTGAATTGAAGCCGGACACGTCCAAAGAGGAGCTGAAAACGCTGGCGGAAAAAATCGGTGTCGATGTTCGCAG
CGCTGAAAGCATTGATGACTATTTCTTTCTGATCTTCATGGATAAAATTGAAAGCCAACTGCCTGCCGATGAGCTGGTGT
TTGTCGAAAAGTACCCTCCGTATCAAGCCGCTCTGGCGCGTTTGACATCGGATGGCTGGGGCGACCGCTTTGAGGCTTAT
TGGAAAGGCATGGAGCTTTGCAATGCCTTCCATGAGCTGAACGACCCCACGGTGCAGCGTCTGCGTTCCCAGGAAGACCT
GCAGAAGAAATCTGAAATGCACAAGGAGCCCGTCAGTCTGGATGATGAGTTCTTCCAGTGCCTGGAGGCTGGACTTCCAC
CTAGTGGTGGCATCGCCTTGGGCGTCGAACGTTTGTTTATGGCCTTGCTGGGGCTGCAAAATATCTCGGACCTTCGTTTG
TTTCCTCAACAGAATTAA

Upstream 100 bases:

>100_bases
GAACGAAGATGTTGTTTACGGTGCGAATGACATGGATTCCATCCTGTCCCAGCTTGATTCCTTGCGTGATCAGGGCCTTT
GCCATTAAAGTGGCAGGGCT

Downstream 100 bases:

>100_bases
GTTCAGTTTTATTATTTCTAATTAAGCTCAGTCTAGTTCGGACCCCAAAGGACTGCCCATCTTTACTGTTCTTTGTCAGT
CTTTCAGAGCGATTAATGTG

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSRNDFLTQIWKPYPAMPENAQAAGRIYQIEREGDALKLVLHRDSETHKIHFEKAPKHSEFLIEGDIVAVVSASEVVLLA
PQKQSLPNRSYNKELTQNWGTYLQSLREFFVSKGFVELKTPSLVVCPGTEPSLDVFSTNLKIGSRQQKLFLPTSPELHLK
KALALGAEKIFELAPCFRNGEVTERHQPEFLMLEWYRAYASLKLIKEDTVKLVSHLAQAMKVAGPVKVTSYSVAELFKIH
CGFELKPDTSKEELKTLAEKIGVDVRSAESIDDYFFLIFMDKIESQLPADELVFVEKYPPYQAALARLTSDGWGDRFEAY
WKGMELCNAFHELNDPTVQRLRSQEDLQKKSEMHKEPVSLDDEFFQCLEAGLPPSGGIALGVERLFMALLGLQNISDLRL
FPQQN

Sequences:

>Translated_405_residues
MSRNDFLTQIWKPYPAMPENAQAAGRIYQIEREGDALKLVLHRDSETHKIHFEKAPKHSEFLIEGDIVAVVSASEVVLLA
PQKQSLPNRSYNKELTQNWGTYLQSLREFFVSKGFVELKTPSLVVCPGTEPSLDVFSTNLKIGSRQQKLFLPTSPELHLK
KALALGAEKIFELAPCFRNGEVTERHQPEFLMLEWYRAYASLKLIKEDTVKLVSHLAQAMKVAGPVKVTSYSVAELFKIH
CGFELKPDTSKEELKTLAEKIGVDVRSAESIDDYFFLIFMDKIESQLPADELVFVEKYPPYQAALARLTSDGWGDRFEAY
WKGMELCNAFHELNDPTVQRLRSQEDLQKKSEMHKEPVSLDDEFFQCLEAGLPPSGGIALGVERLFMALLGLQNISDLRL
FPQQN
>Mature_404_residues
SRNDFLTQIWKPYPAMPENAQAAGRIYQIEREGDALKLVLHRDSETHKIHFEKAPKHSEFLIEGDIVAVVSASEVVLLAP
QKQSLPNRSYNKELTQNWGTYLQSLREFFVSKGFVELKTPSLVVCPGTEPSLDVFSTNLKIGSRQQKLFLPTSPELHLKK
ALALGAEKIFELAPCFRNGEVTERHQPEFLMLEWYRAYASLKLIKEDTVKLVSHLAQAMKVAGPVKVTSYSVAELFKIHC
GFELKPDTSKEELKTLAEKIGVDVRSAESIDDYFFLIFMDKIESQLPADELVFVEKYPPYQAALARLTSDGWGDRFEAYW
KGMELCNAFHELNDPTVQRLRSQEDLQKKSEMHKEPVSLDDEFFQCLEAGLPPSGGIALGVERLFMALLGLQNISDLRLF
PQQN

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=448, Percent_Identity=25.2232142857143, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI5031815, Length=447, Percent_Identity=24.6085011185682, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1790571, Length=318, Percent_Identity=33.0188679245283, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082379, Length=308, Percent_Identity=32.4675324675325, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789256, Length=316, Percent_Identity=31.3291139240506, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17535925, Length=326, Percent_Identity=27.3006134969325, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17535927, Length=326, Percent_Identity=27.3006134969325, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71994340, Length=326, Percent_Identity=27.3006134969325, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320242, Length=456, Percent_Identity=26.0964912280702, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323011, Length=315, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24640851, Length=463, Percent_Identity=27.2138228941685, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24640849, Length=463, Percent_Identity=27.2138228941685, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 45999; Mature: 45868

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNDFLTQIWKPYPAMPENAQAAGRIYQIEREGDALKLVLHRDSETHKIHFEKAPKHSE
CCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCC
FLIEGDIVAVVSASEVVLLAPQKQSLPNRSYNKELTQNWGTYLQSLREFFVSKGFVELKT
EEEECCEEEEEECCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECC
PSLVVCPGTEPSLDVFSTNLKIGSRQQKLFLPTSPELHLKKALALGAEKIFELAPCFRNG
CCEEEECCCCCCHHHEEECCEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EVTERHQPEFLMLEWYRAYASLKLIKEDTVKLVSHLAQAMKVAGPVKVTSYSVAELFKIH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
CGFELKPDTSKEELKTLAEKIGVDVRSAESIDDYFFLIFMDKIESQLPADELVFVEKYPP
CCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCCC
YQAALARLTSDGWGDRFEAYWKGMELCNAFHELNDPTVQRLRSQEDLQKKSEMHKEPVSL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DDEFFQCLEAGLPPSGGIALGVERLFMALLGLQNISDLRLFPQQN
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SRNDFLTQIWKPYPAMPENAQAAGRIYQIEREGDALKLVLHRDSETHKIHFEKAPKHSE
CCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCC
FLIEGDIVAVVSASEVVLLAPQKQSLPNRSYNKELTQNWGTYLQSLREFFVSKGFVELKT
EEEECCEEEEEECCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECC
PSLVVCPGTEPSLDVFSTNLKIGSRQQKLFLPTSPELHLKKALALGAEKIFELAPCFRNG
CCEEEECCCCCCHHHEEECCEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EVTERHQPEFLMLEWYRAYASLKLIKEDTVKLVSHLAQAMKVAGPVKVTSYSVAELFKIH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
CGFELKPDTSKEELKTLAEKIGVDVRSAESIDDYFFLIFMDKIESQLPADELVFVEKYPP
CCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCCC
YQAALARLTSDGWGDRFEAYWKGMELCNAFHELNDPTVQRLRSQEDLQKKSEMHKEPVSL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DDEFFQCLEAGLPPSGGIALGVERLFMALLGLQNISDLRLFPQQN
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; NH4+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.006 {L-Lys}} 0.005 {L-Lys}} 0.0025 {L-Lys}} 0.004 {Lys}} 0.33 {ATP}} 0.037 {ATP}} 0.0057 {tRNA}} 0.002 {tRNA}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: D-Lysine; KCl; L-Lysineamide; L-Lysineethyl ester; L-Lysinehydrazide; L-Lysinemethyl ester; Mg2+; NH4+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA