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The map label for this gene is cspR [C]

Identifier: 42521894

GI number: 42521894

Start: 257544

End: 258026

Strand: Direct

Name: cspR [C]

Synonym: Bd0271

Alternate gene names: 42521894

Gene position: 257544-258026 (Clockwise)

Preceding gene: 42521893

Following gene: 42521895

Centisome position: 6.81

GC content: 53.0

Gene sequence:

>483_bases
TTGCCTAATACAGAAAAGCTTTTCCGCATTGTTCTTATCGAGCCCGAAATCCCGCAAAACACCGGAAATATCGGGCGCAC
GTGTGTTGGCACCAACTGCGAACTGCACATTGTGGGAAAAATGGGCTTTGAAATCAACGACACCAATCTGAAGCGGGCGG
GGCTGGATTACTGGCCTCACCTGACGTGGTATCGCCACGAAACCTTCGAGGACTGGTGGAAACACGTTGAAGATCCGTCC
CGCATCTGGCTTTTCACCACCAAGACCCAGCGCACCTATTTTGAGCCCCAATATCAATCCGGAGACTGGTTCGTTTTTGG
TAAAGAAACCAAAGGACTGGATCCCGAGTTCCTGCAAAAGCACCCACAGCAGACCGTCACCATCCCCATGATTGGGGAGG
GGGCTCGCAGCCTGAATCTCGCCACCAGTGTGGCGATCGCGGCTTATGAGGGTCTTCGTCAGGTGCGATTTACATTGCGA
TAG

Upstream 100 bases:

>100_bases
GATTTGACGCTCTATCGTGAGCTGATGAATCTTGAGCTGATCGTCGATAAAGGTCGTCGACTGGCGCTGTCTAAACTGGG
CCAAGAGGTTTACAGACACC

Downstream 100 bases:

>100_bases
TTTAAGGCTTTGGTCGGGGTCGCTCTCGAGGAAGTCTTTGAAATTCTGAATGAAATCGCGTCAATGACGCATCCAGAAAT
TGCCCCCTTACCTTGCCGCC

Product: rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MPNTEKLFRIVLIEPEIPQNTGNIGRTCVGTNCELHIVGKMGFEINDTNLKRAGLDYWPHLTWYRHETFEDWWKHVEDPS
RIWLFTTKTQRTYFEPQYQSGDWFVFGKETKGLDPEFLQKHPQQTVTIPMIGEGARSLNLATSVAIAAYEGLRQVRFTLR

Sequences:

>Translated_160_residues
MPNTEKLFRIVLIEPEIPQNTGNIGRTCVGTNCELHIVGKMGFEINDTNLKRAGLDYWPHLTWYRHETFEDWWKHVEDPS
RIWLFTTKTQRTYFEPQYQSGDWFVFGKETKGLDPEFLQKHPQQTVTIPMIGEGARSLNLATSVAIAAYEGLRQVRFTLR
>Mature_159_residues
PNTEKLFRIVLIEPEIPQNTGNIGRTCVGTNCELHIVGKMGFEINDTNLKRAGLDYWPHLTWYRHETFEDWWKHVEDPSR
IWLFTTKTQRTYFEPQYQSGDWFVFGKETKGLDPEFLQKHPQQTVTIPMIGEGARSLNLATSVAIAAYEGLRQVRFTLR

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=41.4473684210526, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18688; Mature: 18557

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTEKLFRIVLIEPEIPQNTGNIGRTCVGTNCELHIVGKMGFEINDTNLKRAGLDYWPH
CCCHHHEEEEEEECCCCCCCCCCCCCEEECCCCEEEEEEECCCEECCCCCCCCCCCCCCC
LTWYRHETFEDWWKHVEDPSRIWLFTTKTQRTYFEPQYQSGDWFVFGKETKGLDPEFLQK
CEEEECCCHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCHHHHHC
HPQQTVTIPMIGEGARSLNLATSVAIAAYEGLRQVRFTLR
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
PNTEKLFRIVLIEPEIPQNTGNIGRTCVGTNCELHIVGKMGFEINDTNLKRAGLDYWPH
CCHHHEEEEEEECCCCCCCCCCCCCEEECCCCEEEEEEECCCEECCCCCCCCCCCCCCC
LTWYRHETFEDWWKHVEDPSRIWLFTTKTQRTYFEPQYQSGDWFVFGKETKGLDPEFLQK
CEEEECCCHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCHHHHHC
HPQQTVTIPMIGEGARSLNLATSVAIAAYEGLRQVRFTLR
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]