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The map label for this gene is gadB

Identifier: 41410355

GI number: 41410355

Start: 4726387

End: 4727778

Strand: Direct

Name: gadB

Synonym: MAP4257

Alternate gene names: 41410355

Gene position: 4726387-4727778 (Clockwise)

Preceding gene: 41410354

Following gene: 41410356

Centisome position: 97.86

GC content: 68.03

Gene sequence:

>1392_bases
GTGCCCCAACATCCTTCGCTGCCCGCGCATGCCATCGCCCCGGCCTACACCGGCCGGCTGTTCACCGCGCCGGTGCCCGC
TCTGCGGATGCCCGACGAATCGATGGATCCCGACGCCGCCTACCGGTTCATCCACGACGAGCTGATGCTCGACGGCAGCT
CCCGGCTGAACCTGGCCACCTTCGTCACCACCTGGATGGACCCCGAGGCCGGGCGGTTGATGGCCGAGACATTCGACAAG
AACATGATCGACAAGGACGAATACCCGGCCACCGCGGCCATCGAGCAGCGCTGCGTGTGCATGGTCGCCGACCTGTTCCA
CGCCGAAGGTCTGAACGACGCCGACCCGTACAGCGCCTGCGGGGTGTCCACCATCGGGTCCAGCGAGGCGGTGATGTTGG
GCGGGCTGGCGATGAAGTGGCGGTGGCGCGCCAAGACCAAGGACTGGAAGAAGCGCACCCCGAACCTGGTGATGGGCTCC
AACGTCCAGGTGGTCTGGGAGAAGTTCTGCCGCTACTTCGACGTCGAGCCCCGCTACCTGCCGATGGAGGAGGGCCGCTA
CGTCATCACCCCCGAACAGGTCGTCGACGCCGTCGACGAGGACACCATCGGCGTGGTGGCCATCCTGGGCACCACCTACA
CCGGCGAGCTGGAACCGGTCGCCGACATCTGCGGCGCGCTGGACAAGCTGGCCCTCAAACCAGCATCAGCCGGCGGCGTG
GACGTCCCGGTGCACGTCGACGCCGCCAGCGGCGGGTTCGTGGTGCCGTTCCTGCACCCCGAGCTCAAGTGGGACTTCCG
GCTGCCCCGGGTGGTGTCGATCAACGTCAGCGGGCACAAGTACGGGCTGACCTATCCCGGCGTCGGCTTCGTGGTGTGGC
GCAGCAAGGAATACCTGCCCGACGAGCTGGTGTTCCGGGTCAACTACCTGGGCGGCGACATGCCGACCTTCACGCTGAAC
TTCTCCCGCCCCGGCAACCAGGTGGTCGGCCAGTACTACAACTTCCTGCGGCTGGGCCGCGAGGGCTACACCAAGGTGAT
GCAGACGCTGTCCGGCACGGCGCGCTGGCTGGGCGAGCAGCTGCGGGTCAGCGAGCACTGCGAGCTGATCTCGGACGGCT
CGGCGATCCCGGTGGTCGCCTTCCGGCTGGCCAAGAACCGCGGCTACACCGAGTTCGACGTCTCCCACGAGCTGCGCACC
TACGGCTGGCAGGTGCCCGCCTACACCATGCCCGACAACGCCACCGACGTCTCGGTGCTGCGCATCGTGGTGCGTGAGGG
GCTGTCGGCCGACCTGGCCCGGGCGCTGCACGACGACGCCGTCTCGGCGCTGACCTCGCTGGACAAGGTCAAGCCGGGCG
GCCACTACGACGCCCAGCACTTCGCGCACTGA

Upstream 100 bases:

>100_bases
GACCCCGGCCCCGGCGAGGCGCCCACCTCCTCGTCGCGCATGGTTCCGCACATCCGGGCCGCCCTGGCCGCCCTGTAGAC
CTATAGAGAGGAATTCCCCA

Downstream 100 bases:

>100_bases
GACTTTGCGCCGAGCGTGCAGCCACGGCGATTTTTTCGCGATTTTTTCGCCATGAGTGCACGTTGGGCACTGGGAGAGAG
CACGCGCGCGTTTCTGGGAC

Product: GadB

Products: NA

Alternate protein names: GAD [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPQHPSLPAHAIAPAYTGRLFTAPVPALRMPDESMDPDAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAGRLMAETFDK
NMIDKDEYPATAAIEQRCVCMVADLFHAEGLNDADPYSACGVSTIGSSEAVMLGGLAMKWRWRAKTKDWKKRTPNLVMGS
NVQVVWEKFCRYFDVEPRYLPMEEGRYVITPEQVVDAVDEDTIGVVAILGTTYTGELEPVADICGALDKLALKPASAGGV
DVPVHVDAASGGFVVPFLHPELKWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRSKEYLPDELVFRVNYLGGDMPTFTLN
FSRPGNQVVGQYYNFLRLGREGYTKVMQTLSGTARWLGEQLRVSEHCELISDGSAIPVVAFRLAKNRGYTEFDVSHELRT
YGWQVPAYTMPDNATDVSVLRIVVREGLSADLARALHDDAVSALTSLDKVKPGGHYDAQHFAH

Sequences:

>Translated_463_residues
MPQHPSLPAHAIAPAYTGRLFTAPVPALRMPDESMDPDAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAGRLMAETFDK
NMIDKDEYPATAAIEQRCVCMVADLFHAEGLNDADPYSACGVSTIGSSEAVMLGGLAMKWRWRAKTKDWKKRTPNLVMGS
NVQVVWEKFCRYFDVEPRYLPMEEGRYVITPEQVVDAVDEDTIGVVAILGTTYTGELEPVADICGALDKLALKPASAGGV
DVPVHVDAASGGFVVPFLHPELKWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRSKEYLPDELVFRVNYLGGDMPTFTLN
FSRPGNQVVGQYYNFLRLGREGYTKVMQTLSGTARWLGEQLRVSEHCELISDGSAIPVVAFRLAKNRGYTEFDVSHELRT
YGWQVPAYTMPDNATDVSVLRIVVREGLSADLARALHDDAVSALTSLDKVKPGGHYDAQHFAH
>Mature_462_residues
PQHPSLPAHAIAPAYTGRLFTAPVPALRMPDESMDPDAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAGRLMAETFDKN
MIDKDEYPATAAIEQRCVCMVADLFHAEGLNDADPYSACGVSTIGSSEAVMLGGLAMKWRWRAKTKDWKKRTPNLVMGSN
VQVVWEKFCRYFDVEPRYLPMEEGRYVITPEQVVDAVDEDTIGVVAILGTTYTGELEPVADICGALDKLALKPASAGGVD
VPVHVDAASGGFVVPFLHPELKWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRSKEYLPDELVFRVNYLGGDMPTFTLNF
SRPGNQVVGQYYNFLRLGREGYTKVMQTLSGTARWLGEQLRVSEHCELISDGSAIPVVAFRLAKNRGYTEFDVSHELRTY
GWQVPAYTMPDNATDVSVLRIVVREGLSADLARALHDDAVSALTSLDKVKPGGHYDAQHFAH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=336, Percent_Identity=23.5119047619048, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789934, Length=443, Percent_Identity=48.9841986455982, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787769, Length=443, Percent_Identity=48.9841986455982, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25148342, Length=334, Percent_Identity=28.1437125748503, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17557272, Length=367, Percent_Identity=24.5231607629428, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543922, Length=350, Percent_Identity=24, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323905, Length=465, Percent_Identity=42.1505376344086, Blast_Score=336, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6320500, Length=341, Percent_Identity=25.5131964809384, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355963, Length=353, Percent_Identity=26.0623229461756, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24654344, Length=353, Percent_Identity=26.0623229461756, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 51331; Mature: 51200

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQHPSLPAHAIAPAYTGRLFTAPVPALRMPDESMDPDAAYRFIHDELMLDGSSRLNLAT
CCCCCCCCHHHHCHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEE
FVTTWMDPEAGRLMAETFDKNMIDKDEYPATAAIEQRCVCMVADLFHAEGLNDADPYSAC
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GVSTIGSSEAVMLGGLAMKWRWRAKTKDWKKRTPNLVMGSNVQVVWEKFCRYFDVEPRYL
CCCCCCCCCEEEECCEEEEEEECCCCCHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEE
PMEEGRYVITPEQVVDAVDEDTIGVVAILGTTYTGELEPVADICGALDKLALKPASAGGV
EHHCCCEEECHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DVPVHVDAASGGFVVPFLHPELKWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRSKEYLP
EEEEEEECCCCCEEEEEECCCCEECCCCCEEEEEEECCCEECCCCCCCEEEEECCCCCCC
DELVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGREGYTKVMQTLSGTARWLGEQ
HHHEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LRVSEHCELISDGSAIPVVAFRLAKNRGYTEFDVSHELRTYGWQVPAYTMPDNATDVSVL
HCHHHHHHHHCCCCCCHHHHEEEHHCCCCEEEECCHHHHHCCCCCCEEECCCCCCHHHHH
RIVVREGLSADLARALHDDAVSALTSLDKVKPGGHYDAQHFAH
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
PQHPSLPAHAIAPAYTGRLFTAPVPALRMPDESMDPDAAYRFIHDELMLDGSSRLNLAT
CCCCCCCHHHHCHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEE
FVTTWMDPEAGRLMAETFDKNMIDKDEYPATAAIEQRCVCMVADLFHAEGLNDADPYSAC
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GVSTIGSSEAVMLGGLAMKWRWRAKTKDWKKRTPNLVMGSNVQVVWEKFCRYFDVEPRYL
CCCCCCCCCEEEECCEEEEEEECCCCCHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEE
PMEEGRYVITPEQVVDAVDEDTIGVVAILGTTYTGELEPVADICGALDKLALKPASAGGV
EHHCCCEEECHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DVPVHVDAASGGFVVPFLHPELKWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRSKEYLP
EEEEEEECCCCCEEEEEECCCCEECCCCCEEEEEEECCCEECCCCCCCEEEEECCCCCCC
DELVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGREGYTKVMQTLSGTARWLGEQ
HHHEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LRVSEHCELISDGSAIPVVAFRLAKNRGYTEFDVSHELRTYGWQVPAYTMPDNATDVSVL
HCHHHHHHHHCCCCCCHHHHEEEHHCCCCEEEECCHHHHHCCCCCCEEECCCCCCHHHHH
RIVVREGLSADLARALHDDAVSALTSLDKVKPGGHYDAQHFAH
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411264; 11337471 [H]