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The map label for this gene is rmlB

Identifier: 41410323

GI number: 41410323

Start: 4695320

End: 4696315

Strand: Reverse

Name: rmlB

Synonym: MAP4225c

Alternate gene names: 41410323

Gene position: 4696315-4695320 (Counterclockwise)

Preceding gene: 41410324

Following gene: 41410322

Centisome position: 97.24

GC content: 67.17

Gene sequence:

>996_bases
ATGCGGTTACTGGTCACCGGCGGCGCGGGATTCATCGGCGCCAACTTCGTGCACAGCACCGTTCGCGAGCATCCCGAGGA
CTCGGTGACGGTGCTCGACGCCCTGACCTACGCGGGCCGGCGCGAGTCGCTGGCCGGCGTCGAGGATTCCATCCGGCTGG
TGGTCGGCGACATCACCGACGCCGAGCTGGTGTCGCGGCTGGTCGCCGAGTCCGACGCCGTGGTGCACTTCGCCGCCGAG
AGCCACGTCGACAACGCGCTGGCCGGCCCGGAGCCGTTCCTGCACACCAACGTGGTGGGCACCTTCACCATCCTGGAGGC
GGTGCGGCGTCACGGCGTGCGGCTGCACCACATCTCCACCGACGAGGTGTACGGCGACCTCGAGCTCGACGACCCGAACC
GGTTCACCGAGTCGACGCCGTACAACCCGTCCAGCCCGTACTCGGCCACCAAGGCGGCCGCCGACATGCTGGTGCGCGCC
TGGGTGCGCTCGTACGGGGTGCGCGCGACAATCTCCAACTGCTCCAACAACTACGGCCCGTATCAGCACGTCGAGAAGTT
CATCCCGCGCCAGATCACCAACGTGCTCACCGGCCGCCGCCCCAAGTTGTACGGCACCGGCGCCAACGTGCGCGACTGGA
TCCACGTCGACGACCACAACAGCGCGGTGCGCCGCATCCTGGAATCCGGCGAGATCGGCCGCACCTACCTGATCAGCTCG
GAGGGTGAACGCGACAACCTGACCGTGCTGCGGACGTTGCTGCAGATGATGGGGCGCGACCCCGACGACTTCGACCACGT
CACCGACCGCGTCGGACACGACCTGCGCTACGCTATCGACCCGTCCACGCTGTACGACGAATTATGTTGGGCGCCAAAGC
ACACCGACTTCGAGGAGGGCCTGCGTGAGACCATCGACTGGTATCGCGCGAACGAATCGTGGTGGCGGCCGTTGAAAGAC
GCCTCGGAAGCGCGCTACGAAGAACGCGGGCAGTGA

Upstream 100 bases:

>100_bases
GGATTCCGAGGAGACGCTGCTGCCCGCGCTGACCGAATTGGCCGATGCTCTGGGGCTGACTCGGACGAACTGACCGATCG
GCTCCGTTAGGGTTTGGGTC

Downstream 100 bases:

>100_bases
CGTGTCGGCCCGCGAACTGAAAGTCCCCGGCGCCTGGGAGATCACCCCCACCGTGCACGGCGATGCCCGCGGCCATTTCT
TTGAATGGCTGACCGACAAG

Product: RmlB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRLLVTGGAGFIGANFVHSTVREHPEDSVTVLDALTYAGRRESLAGVEDSIRLVVGDITDAELVSRLVAESDAVVHFAAE
SHVDNALAGPEPFLHTNVVGTFTILEAVRRHGVRLHHISTDEVYGDLELDDPNRFTESTPYNPSSPYSATKAAADMLVRA
WVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGTGANVRDWIHVDDHNSAVRRILESGEIGRTYLISS
EGERDNLTVLRTLLQMMGRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRETIDWYRANESWWRPLKD
ASEARYEERGQ

Sequences:

>Translated_331_residues
MRLLVTGGAGFIGANFVHSTVREHPEDSVTVLDALTYAGRRESLAGVEDSIRLVVGDITDAELVSRLVAESDAVVHFAAE
SHVDNALAGPEPFLHTNVVGTFTILEAVRRHGVRLHHISTDEVYGDLELDDPNRFTESTPYNPSSPYSATKAAADMLVRA
WVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGTGANVRDWIHVDDHNSAVRRILESGEIGRTYLISS
EGERDNLTVLRTLLQMMGRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRETIDWYRANESWWRPLKD
ASEARYEERGQ
>Mature_331_residues
MRLLVTGGAGFIGANFVHSTVREHPEDSVTVLDALTYAGRRESLAGVEDSIRLVVGDITDAELVSRLVAESDAVVHFAAE
SHVDNALAGPEPFLHTNVVGTFTILEAVRRHGVRLHHISTDEVYGDLELDDPNRFTESTPYNPSSPYSATKAAADMLVRA
WVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGTGANVRDWIHVDDHNSAVRRILESGEIGRTYLISS
EGERDNLTVLRTLLQMMGRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRETIDWYRANESWWRPLKD
ASEARYEERGQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=33.033033033033, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI42516563, Length=312, Percent_Identity=26.2820512820513, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4504031, Length=302, Percent_Identity=25.1655629139073, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=47.9768786127168, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=45.0276243093923, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788366, Length=175, Percent_Identity=32.5714285714286, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=34.1538461538462, Blast_Score=167, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI115532424, Length=317, Percent_Identity=30.9148264984227, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17539532, Length=318, Percent_Identity=24.2138364779874, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=192, Percent_Identity=30.2083333333333, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=192, Percent_Identity=30.2083333333333, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=192, Percent_Identity=30.2083333333333, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=192, Percent_Identity=30.2083333333333, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=192, Percent_Identity=30.2083333333333, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=191, Percent_Identity=29.8429319371728, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=245, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356223, Length=312, Percent_Identity=23.7179487179487, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24158427, Length=246, Percent_Identity=27.6422764227642, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37279; Mature: 37279

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLVTGGAGFIGANFVHSTVREHPEDSVTVLDALTYAGRRESLAGVEDSIRLVVGDITD
CEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCH
AELVSRLVAESDAVVHFAAESHVDNALAGPEPFLHTNVVGTFTILEAVRRHGVRLHHIST
HHHHHHHHHCCCCEEEEEHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECH
DEVYGDLELDDPNRFTESTPYNPSSPYSATKAAADMLVRAWVRSYGVRATISNCSNNYGP
HHHEECEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCC
YQHVEKFIPRQITNVLTGRRPKLYGTGANVRDWIHVDDHNSAVRRILESGEIGRTYLISS
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEECC
EGERDNLTVLRTLLQMMGRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEG
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCHHHH
LRETIDWYRANESWWRPLKDASEARYEERGQ
HHHHHHHHHCCHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLLVTGGAGFIGANFVHSTVREHPEDSVTVLDALTYAGRRESLAGVEDSIRLVVGDITD
CEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCH
AELVSRLVAESDAVVHFAAESHVDNALAGPEPFLHTNVVGTFTILEAVRRHGVRLHHIST
HHHHHHHHHCCCCEEEEEHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECH
DEVYGDLELDDPNRFTESTPYNPSSPYSATKAAADMLVRAWVRSYGVRATISNCSNNYGP
HHHEECEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCC
YQHVEKFIPRQITNVLTGRRPKLYGTGANVRDWIHVDDHNSAVRRILESGEIGRTYLISS
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEECC
EGERDNLTVLRTLLQMMGRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEG
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCHHHH
LRETIDWYRANESWWRPLKDASEARYEERGQ
HHHHHHHHHCCHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]