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The map label for this gene is thcD [H]

Identifier: 41410245

GI number: 41410245

Start: 4625117

End: 4626313

Strand: Direct

Name: thcD [H]

Synonym: MAP4147

Alternate gene names: 41410245

Gene position: 4625117-4626313 (Clockwise)

Preceding gene: 41410244

Following gene: 41410247

Centisome position: 95.76

GC content: 71.68

Gene sequence:

>1197_bases
GTGAGTAGTGACGCAAGCGCCAGCAACGGAATCGTGATCGTCGGCGGCGGCCTGGCCGCCGCCCGCACCGCCGAGCAGCT
GCGGCGCTCGGAGTATTCCGGGCCCATCACCATCGTCAGCGACGAGGTGCACCTGCCCTACGACCGCCCGCCGCTGTCCA
AAGAGGTGCTGCGCAAAGAGGTGGACGACACCGCGCTCAAACCCCGCCAGTGGTACGACGACAACGACATCACGCTGCGG
CTGGGGTCGGCCGCGCGCAGCCTGGACACCGCGGCGCAGACGCTGACGCTCGAGGACGGCACCACGCTCGGCTACGACGA
ACTCGTCATCGCCACCGGCCTGGTGCCGCGGCGCATCCCGACCATCCCGGACCTGGACGGCATCCGGGTGCTGCGGACCT
TCGACGAGAGCCTGGCGCTGCGCGAGCACGCGTCCGCGGCGCAGCGCGCGGTGATCATCGGCGCCGGCTTCATCGGCTGC
GAGGTGGCCGCCAGCCTGCGCAGCCTGGGCGTGGACGTGGTGCTGGTCGAGCCGCAACCGACCCCGCTGGCCGCGGTGCT
CGGCGAGCAGATCGGCGAGCTGGTGGCCCGGCTGCACCGCGCCGAGGGCGTCGACGTGCGCCTCGGTGTCGGGGTGGCAC
AGGTGCGCGGCGACACGCACGTCGAGGCGGTGGTGCTGACCGACGGCACCGAGCTGGCGGCCGACGTGGTGGTGATCGGC
ATCGGGTCGCGTCCGGCCACCGATTGGCTCGAGGGCAGCGGCGTGGCGATCGACAGCGTCGACCGGGGCGTCCTGTGCGA
CGAGGCCGGACGCACCAGCGCCCCCAACGTGTGGGCGCTCGGCGACGTCGCGTCGTGGCGCGACGCGACGGGACACCAAG
GGCGGGTGGAACATTGGAGCAACGTCGCGGACCAGGCCCGGGTGGTGGTGCCGGCGATGCTGGGCAAGGAGGTGCCGCCG
GTGGTGGTGGTGCCCTACTTCTGGAGCGACCAATACGACGTCAAGATCCAGTGCCTGGGCGAACCGGAGGCCGACGACAT
CGTCCACATCGTGGAGGACGACGGCCGCAAGTTCCTGGCCTACTACGAACGCGACGGGGCCCTGGTCGGGGTGGTCGGTG
GCGGCATGCCGGGCAAGGTCATGAAGACCCGCGCCAAGATCGCCGCGGCCGTGCCCATCGCCGAGATGCTCGGCTGA

Upstream 100 bases:

>100_bases
TGGCTGGCCGGCGACGGCTCGGGCACGCTGACCGGCACGCAGATCCCCGTTGATAAGGGCGCCCTAAAGTACTGAGCGGC
CATCGTGCTAAGAACTCCAC

Downstream 100 bases:

>100_bases
GCCGACGGCGGGCGTTCAGCCGACGGCGGCCGCGTACCGGTCGCTGGGGACCTCGGTGTTGCGGATCACGGTGGCCAGCT
GCTCGGCGATGCCCGGCGCG

Product: hypothetical protein

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSSDASASNGIVIVGGGLAAARTAEQLRRSEYSGPITIVSDEVHLPYDRPPLSKEVLRKEVDDTALKPRQWYDDNDITLR
LGSAARSLDTAAQTLTLEDGTTLGYDELVIATGLVPRRIPTIPDLDGIRVLRTFDESLALREHASAAQRAVIIGAGFIGC
EVAASLRSLGVDVVLVEPQPTPLAAVLGEQIGELVARLHRAEGVDVRLGVGVAQVRGDTHVEAVVLTDGTELAADVVVIG
IGSRPATDWLEGSGVAIDSVDRGVLCDEAGRTSAPNVWALGDVASWRDATGHQGRVEHWSNVADQARVVVPAMLGKEVPP
VVVVPYFWSDQYDVKIQCLGEPEADDIVHIVEDDGRKFLAYYERDGALVGVVGGGMPGKVMKTRAKIAAAVPIAEMLG

Sequences:

>Translated_398_residues
MSSDASASNGIVIVGGGLAAARTAEQLRRSEYSGPITIVSDEVHLPYDRPPLSKEVLRKEVDDTALKPRQWYDDNDITLR
LGSAARSLDTAAQTLTLEDGTTLGYDELVIATGLVPRRIPTIPDLDGIRVLRTFDESLALREHASAAQRAVIIGAGFIGC
EVAASLRSLGVDVVLVEPQPTPLAAVLGEQIGELVARLHRAEGVDVRLGVGVAQVRGDTHVEAVVLTDGTELAADVVVIG
IGSRPATDWLEGSGVAIDSVDRGVLCDEAGRTSAPNVWALGDVASWRDATGHQGRVEHWSNVADQARVVVPAMLGKEVPP
VVVVPYFWSDQYDVKIQCLGEPEADDIVHIVEDDGRKFLAYYERDGALVGVVGGGMPGKVMKTRAKIAAAVPIAEMLG
>Mature_397_residues
SSDASASNGIVIVGGGLAAARTAEQLRRSEYSGPITIVSDEVHLPYDRPPLSKEVLRKEVDDTALKPRQWYDDNDITLRL
GSAARSLDTAAQTLTLEDGTTLGYDELVIATGLVPRRIPTIPDLDGIRVLRTFDESLALREHASAAQRAVIIGAGFIGCE
VAASLRSLGVDVVLVEPQPTPLAAVLGEQIGELVARLHRAEGVDVRLGVGVAQVRGDTHVEAVVLTDGTELAADVVVIGI
GSRPATDWLEGSGVAIDSVDRGVLCDEAGRTSAPNVWALGDVASWRDATGHQGRVEHWSNVADQARVVVPAMLGKEVPPV
VVVPYFWSDQYDVKIQCLGEPEADDIVHIVEDDGRKFLAYYERDGALVGVVGGGMPGKVMKTRAKIAAAVPIAEMLG

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=378, Percent_Identity=30.6878306878307, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI65787454, Length=378, Percent_Identity=30.6878306878307, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI226437568, Length=378, Percent_Identity=30.6878306878307, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4757732, Length=330, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI22202629, Length=330, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1788892, Length=364, Percent_Identity=34.3406593406593, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1789765, Length=285, Percent_Identity=27.3684210526316, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789065, Length=284, Percent_Identity=27.4647887323944, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082354, Length=262, Percent_Identity=27.0992366412214, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=392, Percent_Identity=30.1020408163265, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32564386, Length=332, Percent_Identity=29.5180722891566, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585130, Length=364, Percent_Identity=29.9450549450549, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI281359715, Length=358, Percent_Identity=30.7262569832402, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281359713, Length=358, Percent_Identity=30.7262569832402, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24639250, Length=358, Percent_Identity=30.7262569832402, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18543267, Length=358, Percent_Identity=30.7262569832402, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24639252, Length=358, Percent_Identity=30.7262569832402, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24639257, Length=358, Percent_Identity=30.7262569832402, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24581020, Length=360, Percent_Identity=28.3333333333333, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28573993, Length=360, Percent_Identity=28.3333333333333, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42224; Mature: 42093

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDASASNGIVIVGGGLAAARTAEQLRRSEYSGPITIVSDEVHLPYDRPPLSKEVLRKE
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCHHHHHHH
VDDTALKPRQWYDDNDITLRLGSAARSLDTAAQTLTLEDGTTLGYDELVIATGLVPRRIP
HHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCCCCCC
TIPDLDGIRVLRTFDESLALREHASAAQRAVIIGAGFIGCEVAASLRSLGVDVVLVEPQP
CCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCC
TPLAAVLGEQIGELVARLHRAEGVDVRLGVGVAQVRGDTHVEAVVLTDGTELAADVVVIG
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEE
IGSRPATDWLEGSGVAIDSVDRGVLCDEAGRTSAPNVWALGDVASWRDATGHQGRVEHWS
CCCCCCCHHHCCCCEEEECCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
NVADQARVVVPAMLGKEVPPVVVVPYFWSDQYDVKIQCLGEPEADDIVHIVEDDGRKFLA
HHHHHHEEEEHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
YYERDGALVGVVGGGMPGKVMKTRAKIAAAVPIAEMLG
EEECCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHC
>Mature Secondary Structure 
SSDASASNGIVIVGGGLAAARTAEQLRRSEYSGPITIVSDEVHLPYDRPPLSKEVLRKE
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCHHHHHHH
VDDTALKPRQWYDDNDITLRLGSAARSLDTAAQTLTLEDGTTLGYDELVIATGLVPRRIP
HHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCCCCCC
TIPDLDGIRVLRTFDESLALREHASAAQRAVIIGAGFIGCEVAASLRSLGVDVVLVEPQP
CCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCC
TPLAAVLGEQIGELVARLHRAEGVDVRLGVGVAQVRGDTHVEAVVLTDGTELAADVVVIG
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEE
IGSRPATDWLEGSGVAIDSVDRGVLCDEAGRTSAPNVWALGDVASWRDATGHQGRVEHWS
CCCCCCCHHHCCCCEEEECCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
NVADQARVVVPAMLGKEVPPVVVVPYFWSDQYDVKIQCLGEPEADDIVHIVEDDGRKFLA
HHHHHHEEEEHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
YYERDGALVGVVGGGMPGKVMKTRAKIAAAVPIAEMLG
EEECCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]