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The map label for this gene is fadB2

Identifier: 41410060

GI number: 41410060

Start: 4422801

End: 4423664

Strand: Direct

Name: fadB2

Synonym: MAP3962

Alternate gene names: 41410060

Gene position: 4422801-4423664 (Clockwise)

Preceding gene: 41410059

Following gene: 41410061

Centisome position: 91.57

GC content: 67.71

Gene sequence:

>864_bases
GTGAGTGACGCAGCGATTCAGCGAGTAGGAGTGGTCGGGGCCGGCCAGATGGGATCCGGCATCGCCGAGGTCTCGGTCCG
CGCCGACGTCGACGTGACGGTGTTCGAGACCACCGAGGCCCTCATCACGGCGGGACGCAACCGCATCGTGAAGTCGCTGG
AGCGGGGCGTGAGCGCCGGCAAGGTCACCGAGCGGGAGCGTGACCGCGCGCTGAGCAAGCTCACCTTCACCACCGACCTG
AAAGACCTCGCCGATCGCCAACTGGTCATCGAGGCCATCATCGAGGACGACGCCGTCAAGGCCCAGGTCTTCGCCGAACT
CGACCGGGTGGTCACCGACCCCGATGCGGTGTTGGCCTCCAACACCTCCAGCATCCCGATCATGAAGCTCGCCGCGGCGA
CCAAGAACCCGCAACGGGTGCTGGGCCTGCACTTCTTCAATCCCGTGCCGGTGCTGCCGCTGGTCGAGCTGGTCAGCACG
CTGGTCACCGACGAGGCCGCCGCCGCGCGCACCGAGGAGTTCGCCAGCGCGGTGCTGGGCAAGCAGGTGGTGCGCTGCAG
CGACCGGTCCGGCTTCGTGGTCAACGCGCTGCTGGTGCCCTACCTGCTGTCGGCGATCCGGATGGTGGAGGCCGGCTTCG
CCACCGTCGAGGACGTGGACAAAGCCGTGGTGGCCGGGCTGTCGCATCCGATGGGCCCGCTGCGGCTTTCCGACCTGGTG
GGCCTGGACACCCTGAAGCTGATCGCGGACAAGATGTTCGAGGAGTTCAAGGAGCCGCAGTACGGCGCCCCGCCGCTGTT
GCTGCGCATGGTGGAGGCCGGCCGGCTCGGCAAGAAGACGGGCCAGGGTTTCTACACGTATTGA

Upstream 100 bases:

>100_bases
CAGACCCAAAAGGACCCTTTTGCACAGCAAAAGCGGTGCCTTTGCGTCTGCTCGCGGCGTGTAAGGTGCGGTGCACGACA
AGACGATTGGCGGAGAAACA

Downstream 100 bases:

>100_bases
CATGTATTGACCGGCGCCCGCGCGGCCGCGCGTGATCAGGTCGGTTACTATGCTCGCGTTCAATAAGACGAAAGGTTTGT
GCCGCGCATGACGGGCTTGG

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSDAAIQRVGVVGAGQMGSGIAEVSVRADVDVTVFETTEALITAGRNRIVKSLERGVSAGKVTERERDRALSKLTFTTDL
KDLADRQLVIEAIIEDDAVKAQVFAELDRVVTDPDAVLASNTSSIPIMKLAAATKNPQRVLGLHFFNPVPVLPLVELVST
LVTDEAAAARTEEFASAVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLV
GLDTLKLIADKMFEEFKEPQYGAPPLLLRMVEAGRLGKKTGQGFYTY

Sequences:

>Translated_287_residues
MSDAAIQRVGVVGAGQMGSGIAEVSVRADVDVTVFETTEALITAGRNRIVKSLERGVSAGKVTERERDRALSKLTFTTDL
KDLADRQLVIEAIIEDDAVKAQVFAELDRVVTDPDAVLASNTSSIPIMKLAAATKNPQRVLGLHFFNPVPVLPLVELVST
LVTDEAAAARTEEFASAVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLV
GLDTLKLIADKMFEEFKEPQYGAPPLLLRMVEAGRLGKKTGQGFYTY
>Mature_286_residues
SDAAIQRVGVVGAGQMGSGIAEVSVRADVDVTVFETTEALITAGRNRIVKSLERGVSAGKVTERERDRALSKLTFTTDLK
DLADRQLVIEAIIEDDAVKAQVFAELDRVVTDPDAVLASNTSSIPIMKLAAATKNPQRVLGLHFFNPVPVLPLVELVSTL
VTDEAAAARTEEFASAVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVG
LDTLKLIADKMFEEFKEPQYGAPPLLLRMVEAGRLGKKTGQGFYTY

Specific function: Catalyzes the NAD-dependent dehydration of beta- hydroxybutyryl-CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5- 6.5. The reverse reaction is able to use NADPH as well as NADH [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=290, Percent_Identity=40.6896551724138, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI296179427, Length=307, Percent_Identity=38.4364820846906, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI20127408, Length=287, Percent_Identity=33.7979094076655, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI261878539, Length=296, Percent_Identity=32.4324324324324, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI68989263, Length=296, Percent_Identity=32.4324324324324, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI115430219, Length=260, Percent_Identity=26.5384615384615, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787661, Length=285, Percent_Identity=41.4035087719298, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1788682, Length=234, Percent_Identity=38.4615384615385, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790281, Length=264, Percent_Identity=32.5757575757576, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17549919, Length=291, Percent_Identity=38.8316151202749, Blast_Score=205, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17553560, Length=288, Percent_Identity=37.5, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17563036, Length=294, Percent_Identity=39.1156462585034, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25144276, Length=284, Percent_Identity=32.3943661971831, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17508951, Length=284, Percent_Identity=32.3943661971831, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17508953, Length=284, Percent_Identity=32.3943661971831, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17558304, Length=283, Percent_Identity=30.7420494699647, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71985923, Length=283, Percent_Identity=27.9151943462898, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71985930, Length=287, Percent_Identity=27.1777003484321, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI193203623, Length=199, Percent_Identity=30.6532663316583, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115533614, Length=199, Percent_Identity=30.6532663316583, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583077, Length=286, Percent_Identity=35.6643356643357, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24583079, Length=286, Percent_Identity=35.6643356643357, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI19921000, Length=286, Percent_Identity=35.6643356643357, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45549573, Length=267, Percent_Identity=28.8389513108614, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129993, Length=248, Percent_Identity=25.4032258064516, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022694
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N [H]

EC number: =1.1.1.157 [H]

Molecular weight: Translated: 30798; Mature: 30667

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAIQRVGVVGAGQMGSGIAEVSVRADVDVTVFETTEALITAGRNRIVKSLERGVSAG
CCCHHHHHHCCEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
KVTERERDRALSKLTFTTDLKDLADRQLVIEAIIEDDAVKAQVFAELDRVVTDPDAVLAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCC
NTSSIPIMKLAAATKNPQRVLGLHFFNPVPVLPLVELVSTLVTDEAAAARTEEFASAVLG
CCCCCCHHHHHHCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
GLDTLKLIADKMFEEFKEPQYGAPPLLLRMVEAGRLGKKTGQGFYTY
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDAAIQRVGVVGAGQMGSGIAEVSVRADVDVTVFETTEALITAGRNRIVKSLERGVSAG
CCHHHHHHCCEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
KVTERERDRALSKLTFTTDLKDLADRQLVIEAIIEDDAVKAQVFAELDRVVTDPDAVLAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCC
NTSSIPIMKLAAATKNPQRVLGLHFFNPVPVLPLVELVSTLVTDEAAAARTEEFASAVLG
CCCCCCHHHHHHCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
GLDTLKLIADKMFEEFKEPQYGAPPLLLRMVEAGRLGKKTGQGFYTY
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]