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The map label for this gene is purA

Identifier: 41409967

GI number: 41409967

Start: 4330421

End: 4331719

Strand: Direct

Name: purA

Synonym: MAP3869

Alternate gene names: 41409967

Gene position: 4330421-4331719 (Clockwise)

Preceding gene: 41409966

Following gene: 41409968

Centisome position: 89.66

GC content: 68.28

Gene sequence:

>1299_bases
ATGCCGGCAATCGTCCTCATCGGCGCCCAGTGGGGCGACGAGGGCAAGGGTAAGGCCACTGACCTGCTCGGTGGGCGCGT
GCAGTGGGTGGTGCGCTACCAGGGCGGCAACAACGCCGGGCACACCGTCGTCTTGCCGACCGGGGAGAACTTCGCCCTGC
ACCTGATCCCGTCCGGGGTGCTCACTCCCGGCGTCACCAACGTCATCGGCAACGGTGTGGTGGTCGACCCCGGGGTGCTG
CTCGACGAGCTCAAGGGGCTCGAAGACCGCGGCGTGGACACCTCGCGGTTGCTGATCTCGGCCGACGCGCACCTGTTGCT
GCCGTATCACGTGGCCATCGACAAGGTCACCGAGCGCTACATGGGCAACAAGAAGATCGGCACCACCGGCCGCGGCATCG
GCCCGTGCTACCAGGACAAGATCGCCCGGATCGGAATCCGGGTGGCCGACGTGCTCGACCGCGAGGTGCTGACCCACAAG
ATCGAGGCGGCGCTGGAGCTGAAGAACCAGATCCTGGTCAAGATCTACAACCGCAAGGCGCTCGATCCGCATCAGGTGGT
CGAATGCCTGCTGGAGCAGGCCGAGGGTTTCCGGCACCGCATCGCCGACACCCGGCTGCTGCTCAACACCGCGCTGGAGG
CCGGCGAGACGGTGCTGTTGGAAGGCTCGCAGGGCACCCTGCTCGACGTCGACCACGGCACGTATCCCTATGTGACGTCG
TCGAATCCGACGGCCGGCGGGGCCGCCGTGGGCTCGGGGATCGGCCCGACCCGGATCACCGCGGTGCTGGGTATCCTCAA
GGCCTACACCACCCGGGTGGGGTCCGGCCCGTTCCCCACCGAGCTGTTCGACGAGAACGGCGAATACCTGTCCAAGACCG
GCGGCGAATTCGGGGTCACCACCGGCCGGCGCCGTCGGTGCGGCTGGTTCGACGCTGTGATCGCCCGCTACGCCACCCGG
GTCAACGGCATCACCGACTTCTTCCTGACCAAGCTCGACGTGCTGTCCAGCCTGGAGACGGTGCCGGTGTGCGTCGGCTA
CCGCATCGACGGGGCGCGCGTCAACGAGATGCCGATGACCCAGAGCGATCTGCACCGCGCCGAGCCGATCTATGAGGAGC
TGCCCGGCTGGTGGGAGGACATCTCGGCGGCCCGCGAGTTCGACGACCTGCCCGCCAAGGCGCGCGACTACGTGCTGCGG
CTGGAAGAGCTTGCCGGAGCGCATGTTTCGTGCATCGGGGTGGGGCCCGGTCGGGAACAGGCCATCGTGCGCCGCGACAT
CCTGGCGGCGCGCCCGTGA

Upstream 100 bases:

>100_bases
GGCTCCTGCGCCGGCCATCGGCGGCCCGCCTCGCCGCCAGGCTAGGGTGGGTCACCGGCTGGACCACAATACGCAGGCGA
GATCAGGGTTGGAGCGTGTC

Downstream 100 bases:

>100_bases
CGGAACCCGACCCGACCGAACTAGATCCCGAATACGAACACCACGGCGGCTTCCCGGAGTACGGCCCGGCCAGCCCCGGG
CCGGGGTTCGCCCGGTTCGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGVLTPGVTNVIGNGVVVDPGVL
LDELKGLEDRGVDTSRLLISADAHLLLPYHVAIDKVTERYMGNKKIGTTGRGIGPCYQDKIARIGIRVADVLDREVLTHK
IEAALELKNQILVKIYNRKALDPHQVVECLLEQAEGFRHRIADTRLLLNTALEAGETVLLEGSQGTLLDVDHGTYPYVTS
SNPTAGGAAVGSGIGPTRITAVLGILKAYTTRVGSGPFPTELFDENGEYLSKTGGEFGVTTGRRRRCGWFDAVIARYATR
VNGITDFFLTKLDVLSSLETVPVCVGYRIDGARVNEMPMTQSDLHRAEPIYEELPGWWEDISAAREFDDLPAKARDYVLR
LEELAGAHVSCIGVGPGREQAIVRRDILAARP

Sequences:

>Translated_432_residues
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGVLTPGVTNVIGNGVVVDPGVL
LDELKGLEDRGVDTSRLLISADAHLLLPYHVAIDKVTERYMGNKKIGTTGRGIGPCYQDKIARIGIRVADVLDREVLTHK
IEAALELKNQILVKIYNRKALDPHQVVECLLEQAEGFRHRIADTRLLLNTALEAGETVLLEGSQGTLLDVDHGTYPYVTS
SNPTAGGAAVGSGIGPTRITAVLGILKAYTTRVGSGPFPTELFDENGEYLSKTGGEFGVTTGRRRRCGWFDAVIARYATR
VNGITDFFLTKLDVLSSLETVPVCVGYRIDGARVNEMPMTQSDLHRAEPIYEELPGWWEDISAAREFDDLPAKARDYVLR
LEELAGAHVSCIGVGPGREQAIVRRDILAARP
>Mature_431_residues
PAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGVLTPGVTNVIGNGVVVDPGVLL
DELKGLEDRGVDTSRLLISADAHLLLPYHVAIDKVTERYMGNKKIGTTGRGIGPCYQDKIARIGIRVADVLDREVLTHKI
EAALELKNQILVKIYNRKALDPHQVVECLLEQAEGFRHRIADTRLLLNTALEAGETVLLEGSQGTLLDVDHGTYPYVTSS
NPTAGGAAVGSGIGPTRITAVLGILKAYTTRVGSGPFPTELFDENGEYLSKTGGEFGVTTGRRRRCGWFDAVIARYATRV
NGITDFFLTKLDVLSSLETVPVCVGYRIDGARVNEMPMTQSDLHRAEPIYEELPGWWEDISAAREFDDLPAKARDYVLRL
EELAGAHVSCIGVGPGREQAIVRRDILAARP

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=43.7939110070258, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=43.8228438228438, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI40316944, Length=400, Percent_Identity=42.75, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=52.2144522144522, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25146555, Length=435, Percent_Identity=44.367816091954, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=44.4700460829493, Blast_Score=341, Evalue=5e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=41.8604651162791, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=43.6018957345972, Blast_Score=330, Evalue=1e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46776; Mature: 46645

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGV
CCEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVVDPGVLLDELKGLEDRGVDTSRLLISADAHLLLPYHVAIDKVTERY
CCCCHHHHHCCCEEECCCHHHHHHCCCHHCCCCHHEEEEECCCEEEEHHHHHHHHHHHHH
MGNKKIGTTGRGIGPCYQDKIARIGIRVADVLDREVLTHKIEAALELKNQILVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
LDPHQVVECLLEQAEGFRHRIADTRLLLNTALEAGETVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGAAVGSGIGPTRITAVLGILKAYTTRVGSGPFPTELFDENGEYLSKTGGEFGVT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHCCCCCCCC
TGRRRRCGWFDAVIARYATRVNGITDFFLTKLDVLSSLETVPVCVGYRIDGARVNEMPMT
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
QSDLHRAEPIYEELPGWWEDISAAREFDDLPAKARDYVLRLEELAGAHVSCIGVGPGREQ
HHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHH
AIVRRDILAARP
HHHHHHHHHCCC
>Mature Secondary Structure 
PAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGV
CEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVVDPGVLLDELKGLEDRGVDTSRLLISADAHLLLPYHVAIDKVTERY
CCCCHHHHHCCCEEECCCHHHHHHCCCHHCCCCHHEEEEECCCEEEEHHHHHHHHHHHHH
MGNKKIGTTGRGIGPCYQDKIARIGIRVADVLDREVLTHKIEAALELKNQILVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
LDPHQVVECLLEQAEGFRHRIADTRLLLNTALEAGETVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGAAVGSGIGPTRITAVLGILKAYTTRVGSGPFPTELFDENGEYLSKTGGEFGVT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHCCCCCCCC
TGRRRRCGWFDAVIARYATRVNGITDFFLTKLDVLSSLETVPVCVGYRIDGARVNEMPMT
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
QSDLHRAEPIYEELPGWWEDISAAREFDDLPAKARDYVLRLEELAGAHVSCIGVGPGREQ
HHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHH
AIVRRDILAARP
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA