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The map label for this gene is fadD28

Identifier: 41409850

GI number: 41409850

Start: 4189503

End: 4191239

Strand: Direct

Name: fadD28

Synonym: MAP3752

Alternate gene names: 41409850

Gene position: 4189503-4191239 (Clockwise)

Preceding gene: 41409849

Following gene: 41409851

Centisome position: 86.74

GC content: 60.05

Gene sequence:

>1737_bases
GTGTTTGGTTCTTCTGTTCAGGACGTGCTGCGTGAGCGCGCCGGCATGCAGCCCAATGACGCCGCATTTACTTTTGTTGA
TTACGAACATGATTGGGCTGGCATTGCCGAAACCCTGACTTGGTCCCAGATGTATCGGCGAACGTTGAACGTTGCACACG
AGCTTAGGCATTGCACATCGAGCGGCGACCGCGCGGTGATATTGGCACCACAGGGCCTCGACCACATCGCCGCATTTCTG
GGAGCGTTGGAGGCTGGCCTCATCGCGGTCCCGCTTGCGCCTCCTGCGGGCGGCGCCCACGATGAGCGGGTCCACTCGGT
GTTGCACGACACATCGCCCTCCGTGCTCCTCGCAACCTCATCAGTCGTCGGCGATGTCGCCAAGTACGCTCAGCCGCGGA
GCGATGGATCCGGTCCTTCGGTGATCGAGATCGATTCACTGGACCTGCAAAGATCTCGAGTGACTTTCAGGCGCGGTAAT
CGACCGGCGACCGCCTATTTGCAATACACCTCGGGGTCCACCCGCCGACCAGCCGGGGTGATGGTCTCGCACAAGAATCT
TCTGGCCAATTTCCGTCAGATCATGTCCGACTACTTCCTGGACTACGGGAAGGTGGCCCCGCCGGATACCACGACCGTGT
CGTGGCTGCCGTTTTATCACGACTTGGGTTTAATTTTAGGGATCTGTGCGCCGATTTTGGCCGGAGTCCGCAGTGTTTTT
ACTAGTCCGGTGGCCTTCTTACAGCGCCCGGCGCGATGGATGCAGTTGCTGGCCAACAATAGTCGGGTCTTTTCGGCGGC
GCCAAACTTCGCTTTCGACTTGGCGGCGAGAAAAACGACTGACGAGGACATGGCCGGGCTGGATTTAAGGGACGTGCTCA
TTATCCAAAGCGGCGCAGAGCGGGTGCAGCCCGCCTCGATGCGGCGATTCCAAGACCGGTTCGCGAAGTTCAACCTCCGC
GACACGGTGATACGGCCTTCCTATGGGCTCGCCGAAGCGACTGTTTACGTCGCGACCCGCACACCGGCGCTACCGCCCCG
AGTCGTCTACTTTCAGCCCGAAATCCTATCTTCCGGACACGCGAAGGGGTGCGAGAGCGGAAGCGGCACCGCGTTGGTCA
GTTACGGTGTGCCACGGTCGCAGACGATCCGCATCGTCGATCCCGAAACCAGCACTGAGTGCCCACCGGGAACGGTCGGC
GAGATCTGGGTGCATGGTGAGAACGTTTCGGCGGGCTATTGGCAGCGACCTCACGAGACTGAAAAGACCTTCGGCGCAAG
GCTTATTGGTCCGTCGGCCGGCACACCACAAGGGCCTTGGTTGAGGACCGGGGACTCGGGCTTCTTCTTCGACGGCGAAT
TATTCATCATCGGGCGTATGAAAGATCTGTTGATTGTGTACGGACGCAACCATTCTCCCGACGACATCGAATCGACGGTA
CAAGCGATCGCACCCGGGCGGTGTGCGGCGGTAGCGGTTCCGGCTGAGGGCACCGAAAAAGTTGTGGTAATCGTCGAATC
CAAGAAACGCGGTGGCTCAGACGAGGAAGTAATGAACAACCTCGCCGCCGTCAAACGCGAACTTACTTCGGCGATTTCGA
ATTCGCACGGCCTCGCCGTGGCGGACGTCGTCCTGGTTGCGCCCGGCTCGATTCCCATCACCACCAGCGGCAAGGTCAGG
CGAGCGACGTGCGTCGAGCAGTACAAGCAGGACCAGTTCGCCCGTCTGGACGTGTAG

Upstream 100 bases:

>100_bases
CCGCCAAGCCTGCGTCACCCCTCACCTCGTGTCTTTACAATCTGCATGACACCCGATTGGTCGTCGCCGCATCGATGACG
AGGAAGGTAACGAGATATCT

Downstream 100 bases:

>100_bases
GGTGCTCAAGAACCAGGCAGGTGCGCAATCGCTTTCATCTCAGCTGTTCTGCACGATGAAGTCAGCCAAACCCATGATTG
ATCTGTGGCGGTCGTGATGC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MFGSSVQDVLRERAGMQPNDAAFTFVDYEHDWAGIAETLTWSQMYRRTLNVAHELRHCTSSGDRAVILAPQGLDHIAAFL
GALEAGLIAVPLAPPAGGAHDERVHSVLHDTSPSVLLATSSVVGDVAKYAQPRSDGSGPSVIEIDSLDLQRSRVTFRRGN
RPATAYLQYTSGSTRRPAGVMVSHKNLLANFRQIMSDYFLDYGKVAPPDTTTVSWLPFYHDLGLILGICAPILAGVRSVF
TSPVAFLQRPARWMQLLANNSRVFSAAPNFAFDLAARKTTDEDMAGLDLRDVLIIQSGAERVQPASMRRFQDRFAKFNLR
DTVIRPSYGLAEATVYVATRTPALPPRVVYFQPEILSSGHAKGCESGSGTALVSYGVPRSQTIRIVDPETSTECPPGTVG
EIWVHGENVSAGYWQRPHETEKTFGARLIGPSAGTPQGPWLRTGDSGFFFDGELFIIGRMKDLLIVYGRNHSPDDIESTV
QAIAPGRCAAVAVPAEGTEKVVVIVESKKRGGSDEEVMNNLAAVKRELTSAISNSHGLAVADVVLVAPGSIPITTSGKVR
RATCVEQYKQDQFARLDV

Sequences:

>Translated_578_residues
MFGSSVQDVLRERAGMQPNDAAFTFVDYEHDWAGIAETLTWSQMYRRTLNVAHELRHCTSSGDRAVILAPQGLDHIAAFL
GALEAGLIAVPLAPPAGGAHDERVHSVLHDTSPSVLLATSSVVGDVAKYAQPRSDGSGPSVIEIDSLDLQRSRVTFRRGN
RPATAYLQYTSGSTRRPAGVMVSHKNLLANFRQIMSDYFLDYGKVAPPDTTTVSWLPFYHDLGLILGICAPILAGVRSVF
TSPVAFLQRPARWMQLLANNSRVFSAAPNFAFDLAARKTTDEDMAGLDLRDVLIIQSGAERVQPASMRRFQDRFAKFNLR
DTVIRPSYGLAEATVYVATRTPALPPRVVYFQPEILSSGHAKGCESGSGTALVSYGVPRSQTIRIVDPETSTECPPGTVG
EIWVHGENVSAGYWQRPHETEKTFGARLIGPSAGTPQGPWLRTGDSGFFFDGELFIIGRMKDLLIVYGRNHSPDDIESTV
QAIAPGRCAAVAVPAEGTEKVVVIVESKKRGGSDEEVMNNLAAVKRELTSAISNSHGLAVADVVLVAPGSIPITTSGKVR
RATCVEQYKQDQFARLDV
>Mature_578_residues
MFGSSVQDVLRERAGMQPNDAAFTFVDYEHDWAGIAETLTWSQMYRRTLNVAHELRHCTSSGDRAVILAPQGLDHIAAFL
GALEAGLIAVPLAPPAGGAHDERVHSVLHDTSPSVLLATSSVVGDVAKYAQPRSDGSGPSVIEIDSLDLQRSRVTFRRGN
RPATAYLQYTSGSTRRPAGVMVSHKNLLANFRQIMSDYFLDYGKVAPPDTTTVSWLPFYHDLGLILGICAPILAGVRSVF
TSPVAFLQRPARWMQLLANNSRVFSAAPNFAFDLAARKTTDEDMAGLDLRDVLIIQSGAERVQPASMRRFQDRFAKFNLR
DTVIRPSYGLAEATVYVATRTPALPPRVVYFQPEILSSGHAKGCESGSGTALVSYGVPRSQTIRIVDPETSTECPPGTVG
EIWVHGENVSAGYWQRPHETEKTFGARLIGPSAGTPQGPWLRTGDSGFFFDGELFIIGRMKDLLIVYGRNHSPDDIESTV
QAIAPGRCAAVAVPAEGTEKVVVIVESKKRGGSDEEVMNNLAAVKRELTSAISNSHGLAVADVVLVAPGSIPITTSGKVR
RATCVEQYKQDQFARLDV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=607, Percent_Identity=25.5354200988468, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI45827692, Length=602, Percent_Identity=24.7508305647841, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI225735629, Length=602, Percent_Identity=24.7508305647841, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI55749758, Length=587, Percent_Identity=26.2350936967632, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI225735625, Length=619, Percent_Identity=24.232633279483, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI45827694, Length=549, Percent_Identity=25.1366120218579, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI45827696, Length=520, Percent_Identity=25.3846153846154, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI225735627, Length=520, Percent_Identity=25.3846153846154, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI156151445, Length=409, Percent_Identity=23.960880195599, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=25.9191176470588, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193202679, Length=618, Percent_Identity=25.0809061488673, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=501, Percent_Identity=23.5528942115768, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=341, Percent_Identity=28.7390029325513, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=544, Percent_Identity=23.8970588235294, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859661, Length=411, Percent_Identity=25.7907542579075, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24654656, Length=470, Percent_Identity=22.7659574468085, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62663; Mature: 62663

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGSSVQDVLRERAGMQPNDAAFTFVDYEHDWAGIAETLTWSQMYRRTLNVAHELRHCTS
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRAVILAPQGLDHIAAFLGALEAGLIAVPLAPPAGGAHDERVHSVLHDTSPSVLLATS
CCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEH
SVVGDVAKYAQPRSDGSGPSVIEIDSLDLQRSRVTFRRGNRPATAYLQYTSGSTRRPAGV
HHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
MVSHKNLLANFRQIMSDYFLDYGKVAPPDTTTVSWLPFYHDLGLILGICAPILAGVRSVF
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
TSPVAFLQRPARWMQLLANNSRVFSAAPNFAFDLAARKTTDEDMAGLDLRDVLIIQSGAE
HHHHHHHHCHHHHHHHHHCCCEEEECCCCCEEEHHHCCCCCCHHCCCCHHEEEEECCCHH
RVQPASMRRFQDRFAKFNLRDTVIRPSYGLAEATVYVATRTPALPPRVVYFQPEILSSGH
HCCHHHHHHHHHHHHCCCCHHEEECCCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCC
AKGCESGSGTALVSYGVPRSQTIRIVDPETSTECPPGTVGEIWVHGENVSAGYWQRPHET
CCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHH
EKTFGARLIGPSAGTPQGPWLRTGDSGFFFDGELFIIGRMKDLLIVYGRNHSPDDIESTV
HHHHCCEEECCCCCCCCCCEEEECCCCEEECCCEEEEECCCEEEEEECCCCCCHHHHHHH
QAIAPGRCAAVAVPAEGTEKVVVIVESKKRGGSDEEVMNNLAAVKRELTSAISNSHGLAV
HHHCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
ADVVLVAPGSIPITTSGKVRRATCVEQYKQDQFARLDV
EEEEEECCCCEEECCCCCCHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MFGSSVQDVLRERAGMQPNDAAFTFVDYEHDWAGIAETLTWSQMYRRTLNVAHELRHCTS
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRAVILAPQGLDHIAAFLGALEAGLIAVPLAPPAGGAHDERVHSVLHDTSPSVLLATS
CCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEH
SVVGDVAKYAQPRSDGSGPSVIEIDSLDLQRSRVTFRRGNRPATAYLQYTSGSTRRPAGV
HHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
MVSHKNLLANFRQIMSDYFLDYGKVAPPDTTTVSWLPFYHDLGLILGICAPILAGVRSVF
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
TSPVAFLQRPARWMQLLANNSRVFSAAPNFAFDLAARKTTDEDMAGLDLRDVLIIQSGAE
HHHHHHHHCHHHHHHHHHCCCEEEECCCCCEEEHHHCCCCCCHHCCCCHHEEEEECCCHH
RVQPASMRRFQDRFAKFNLRDTVIRPSYGLAEATVYVATRTPALPPRVVYFQPEILSSGH
HCCHHHHHHHHHHHHCCCCHHEEECCCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCC
AKGCESGSGTALVSYGVPRSQTIRIVDPETSTECPPGTVGEIWVHGENVSAGYWQRPHET
CCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHH
EKTFGARLIGPSAGTPQGPWLRTGDSGFFFDGELFIIGRMKDLLIVYGRNHSPDDIESTV
HHHHCCEEECCCCCCCCCCEEEECCCCEEECCCEEEEECCCEEEEEECCCCCCHHHHHHH
QAIAPGRCAAVAVPAEGTEKVVVIVESKKRGGSDEEVMNNLAAVKRELTSAISNSHGLAV
HHHCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
ADVVLVAPGSIPITTSGKVRRATCVEQYKQDQFARLDV
EEEEEECCCCEEECCCCCCHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972; 1527058 [H]