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The map label for this gene is trmB

Identifier: 41409741

GI number: 41409741

Start: 4044839

End: 4045618

Strand: Reverse

Name: trmB

Synonym: MAP3643c

Alternate gene names: 41409741

Gene position: 4045618-4044839 (Counterclockwise)

Preceding gene: 41409745

Following gene: 41409740

Centisome position: 83.76

GC content: 70.26

Gene sequence:

>780_bases
ATGGGCCACCATGGACAAATGCATGCGCAGCACGGGGTGACGATGCCTGCCGACTCACCGATCGGGCAACCGGACCGCTC
CGAGCAGCGTTATTTCCCGGCCACGGCGTTCCGCACCCGGCGATCCACCCTGTCCGACGCCCAGCGCCAGACCTGGGACC
GCCGCTGGCCCGAACTGGGCCTGTCGGTGGGCCCGGCCGAGGATCCCGACCGCCCCGGGCCGCCGCTGGACACCGACGCC
TGGTTCGGCCGCAAGGCCCCGGTGGTACTCGAAATCGGTTGTGGCAACGGCACTTCCACGCTCGCGATGGCGAAGGAGGA
GCCCGGCGTGGATGTGATCGCGGTGGAGGTCTACCGGCGCGGCCTGGCGCAGCTGCTGTGCGCGATCGACCGCGACAACG
TGACAAACATCCGGCTGATCCGCGGCAACGCCCTCGACGTGCTGCAGCGGCTCATCGCGCCGGCGTCGCTGACCGGGGTG
CGGGTGTTCTTCCCCGATCCGTGGCCCAAGGCGCGGCACCACAAACGAAGGTTCCTGCAGCCGGGCACGGTTGGCCTGAT
CGCCGACCGGCTACTCCCTGGTGGTGTCCTGCACGTCGCGACGGACCACGCCGGCTACGCCGAGCACATCGCCGACGTCG
GCGCGGGCGAGCCCCGGCTGCGCCCGGCCAACCCGGACAGCCCGCTGCCGATCTCGGTCGCCCGCCCGACCACCAAGTAC
GAGACGAAGGCTCAGGACGCGGGCAGTGCGGTCACCGAATTCATTTGGCTTAGACGGTGA

Upstream 100 bases:

>100_bases
GCACTCACGTCGTCAAGATGCGAGCCACCGTCTATGAGTTACCAGCTCAAGGCAACTGTTGGGTATCAATCCGGACCAGG
GGCGGCTACATCCGGGGTTT

Downstream 100 bases:

>100_bases
ACAGCTTCGACGAGATGAGTAGGCGATGAGGTATGAGCCTGACTGAAGCGACCGCCGCGGGCGCCGAACCCGTCGCGCCG
CCGGCGGTGCTGTCCGGTGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MGHHGQMHAQHGVTMPADSPIGQPDRSEQRYFPATAFRTRRSTLSDAQRQTWDRRWPELGLSVGPAEDPDRPGPPLDTDA
WFGRKAPVVLEIGCGNGTSTLAMAKEEPGVDVIAVEVYRRGLAQLLCAIDRDNVTNIRLIRGNALDVLQRLIAPASLTGV
RVFFPDPWPKARHHKRRFLQPGTVGLIADRLLPGGVLHVATDHAGYAEHIADVGAGEPRLRPANPDSPLPISVARPTTKY
ETKAQDAGSAVTEFIWLRR

Sequences:

>Translated_259_residues
MGHHGQMHAQHGVTMPADSPIGQPDRSEQRYFPATAFRTRRSTLSDAQRQTWDRRWPELGLSVGPAEDPDRPGPPLDTDA
WFGRKAPVVLEIGCGNGTSTLAMAKEEPGVDVIAVEVYRRGLAQLLCAIDRDNVTNIRLIRGNALDVLQRLIAPASLTGV
RVFFPDPWPKARHHKRRFLQPGTVGLIADRLLPGGVLHVATDHAGYAEHIADVGAGEPRLRPANPDSPLPISVARPTTKY
ETKAQDAGSAVTEFIWLRR
>Mature_258_residues
GHHGQMHAQHGVTMPADSPIGQPDRSEQRYFPATAFRTRRSTLSDAQRQTWDRRWPELGLSVGPAEDPDRPGPPLDTDAW
FGRKAPVVLEIGCGNGTSTLAMAKEEPGVDVIAVEVYRRGLAQLLCAIDRDNVTNIRLIRGNALDVLQRLIAPASLTGVR
VFFPDPWPKARHHKRRFLQPGTVGLIADRLLPGGVLHVATDHAGYAEHIADVGAGEPRLRPANPDSPLPISVARPTTKYE
TKAQDAGSAVTEFIWLRR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=226, Percent_Identity=38.0530973451327, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 28412; Mature: 28280

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHHGQMHAQHGVTMPADSPIGQPDRSEQRYFPATAFRTRRSTLSDAQRQTWDRRWPELG
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSVGPAEDPDRPGPPLDTDAWFGRKAPVVLEIGCGNGTSTLAMAKEEPGVDVIAVEVYRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEHHHHHH
GLAQLLCAIDRDNVTNIRLIRGNALDVLQRLIAPASLTGVRVFFPDPWPKARHHKRRFLQ
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCC
PGTVGLIADRLLPGGVLHVATDHAGYAEHIADVGAGEPRLRPANPDSPLPISVARPTTKY
CCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCH
ETKAQDAGSAVTEFIWLRR
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GHHGQMHAQHGVTMPADSPIGQPDRSEQRYFPATAFRTRRSTLSDAQRQTWDRRWPELG
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSVGPAEDPDRPGPPLDTDAWFGRKAPVVLEIGCGNGTSTLAMAKEEPGVDVIAVEVYRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEHHHHHH
GLAQLLCAIDRDNVTNIRLIRGNALDVLQRLIAPASLTGVRVFFPDPWPKARHHKRRFLQ
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCC
PGTVGLIADRLLPGGVLHVATDHAGYAEHIADVGAGEPRLRPANPDSPLPISVARPTTKY
CCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCH
ETKAQDAGSAVTEFIWLRR
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA