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The map label for this gene is ephF

Identifier: 41409648

GI number: 41409648

Start: 3952013

End: 3952903

Strand: Direct

Name: ephF

Synonym: MAP3550

Alternate gene names: 41409648

Gene position: 3952013-3952903 (Clockwise)

Preceding gene: 41409647

Following gene: 41409651

Centisome position: 81.83

GC content: 69.02

Gene sequence:

>891_bases
ATGGTCACCATGCCCGAGCTCGACGGCGTCGAACACCGCTACGTGGATCTCGGAGACGACGTGACGATCCATGTCGCGGA
CGCGGGTCCGGCGAGCGGCCCGGCGGTGATGCTGGTGCACGGCTTCCCGCAGAACTGGTGGGAGTGGCGCGCGTTGATCG
GGCCGCTGGCCGCCGACGGCTACCGGGTGCTGTGTCCGGACCTGCGCGGCGCCGGGTGGAGTTCGGCGCCGCGGTCGAGT
TACCGCAAGGACGAGATGGCCGACGACCTGGCCGGGGTGCTCGATCGCCTGGGCGTCCGGTCGGTCGACCTGGTGGCCCA
CGACTGGGGCGGACCGGTCGCGTTCATCATGATGCTGCGCCACCCGGAAAAGGTGACCGGCTTCTTCGGGGTGAACACCT
CGGCGCCGTTCGTGAAGCGCTCGCTGTCGACGATCCGCAATGTGTGGCGGTTCTGGTATCAGATCCCGATCTCGTTGCCG
GTCATCGGCCCCCGCGTGATCAGCACCCCGGATTCCCGGTTCGTGCGGCTGCTGGGGTCCTGGGTCGGCGGCGGCTACAC
GCTGCCCGAGGAGGATGTCCGCCTCTACCTCGAGTGCATGCGCCAACCGGGGCACGCCGAGGCGGGCTCGCGGTGGTATC
GGTCGTTTCAGACCAGGGAGATGCTGAGCTGGATGCGCGGGGAGTACGACGGCGCCCGCGTGCACGTTCCGGTGCGCTGG
CTGTCGGGCACCGAGGACCCGGTGCTCACCCCGGACCTGTTGGACGGATACGCCGAGCGGATCGACGACTTTCAGCTGGA
GCTGGTCGACGGCGTCGGGCATTGGATCGTCGACCAGCGGCCCGATCTGGTGCTGGACCGGGTGCGGGCCTTCCTGCGCC
GCGAAACTTAA

Upstream 100 bases:

>100_bases
AGATGTTCGGGTTGGACGAGGAGAGCCTGCGCGAGCGTTTCGCCCCCTACGTCGAGCGGTTCCTGGCCTAATCCCGCGCC
GGCGGCTACTTTCTCAACAC

Downstream 100 bases:

>100_bases
ACTAAGGCGCCGGTTTTCGGCGTGTCGTGTGCGTGACGTAAGTGTCGTGGCGTGCCGGGCGGTACGCGCCGCGCGCCCTA
GTCGACGCCGATGGTGACCT

Product: EphF

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MVTMPELDGVEHRYVDLGDDVTIHVADAGPASGPAVMLVHGFPQNWWEWRALIGPLAADGYRVLCPDLRGAGWSSAPRSS
YRKDEMADDLAGVLDRLGVRSVDLVAHDWGGPVAFIMMLRHPEKVTGFFGVNTSAPFVKRSLSTIRNVWRFWYQIPISLP
VIGPRVISTPDSRFVRLLGSWVGGGYTLPEEDVRLYLECMRQPGHAEAGSRWYRSFQTREMLSWMRGEYDGARVHVPVRW
LSGTEDPVLTPDLLDGYAERIDDFQLELVDGVGHWIVDQRPDLVLDRVRAFLRRET

Sequences:

>Translated_296_residues
MVTMPELDGVEHRYVDLGDDVTIHVADAGPASGPAVMLVHGFPQNWWEWRALIGPLAADGYRVLCPDLRGAGWSSAPRSS
YRKDEMADDLAGVLDRLGVRSVDLVAHDWGGPVAFIMMLRHPEKVTGFFGVNTSAPFVKRSLSTIRNVWRFWYQIPISLP
VIGPRVISTPDSRFVRLLGSWVGGGYTLPEEDVRLYLECMRQPGHAEAGSRWYRSFQTREMLSWMRGEYDGARVHVPVRW
LSGTEDPVLTPDLLDGYAERIDDFQLELVDGVGHWIVDQRPDLVLDRVRAFLRRET
>Mature_296_residues
MVTMPELDGVEHRYVDLGDDVTIHVADAGPASGPAVMLVHGFPQNWWEWRALIGPLAADGYRVLCPDLRGAGWSSAPRSS
YRKDEMADDLAGVLDRLGVRSVDLVAHDWGGPVAFIMMLRHPEKVTGFFGVNTSAPFVKRSLSTIRNVWRFWYQIPISLP
VIGPRVISTPDSRFVRLLGSWVGGGYTLPEEDVRLYLECMRQPGHAEAGSRWYRSFQTREMLSWMRGEYDGARVHVPVRW
LSGTEDPVLTPDLLDGYAERIDDFQLELVDGVGHWIVDQRPDLVLDRVRAFLRRET

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=301, Percent_Identity=27.5747508305648, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI27597073, Length=307, Percent_Identity=27.3615635179153, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI218777840, Length=291, Percent_Identity=27.4914089347079, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI13376164, Length=291, Percent_Identity=27.4914089347079, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554012, Length=296, Percent_Identity=25, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562298, Length=301, Percent_Identity=22.5913621262458, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324392, Length=275, Percent_Identity=22.5454545454545, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33486; Mature: 33486

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMPELDGVEHRYVDLGDDVTIHVADAGPASGPAVMLVHGFPQNWWEWRALIGPLAADG
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCC
YRVLCPDLRGAGWSSAPRSSYRKDEMADDLAGVLDRLGVRSVDLVAHDWGGPVAFIMMLR
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
HPEKVTGFFGVNTSAPFVKRSLSTIRNVWRFWYQIPISLPVIGPRVISTPDSRFVRLLGS
CCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
WVGGGYTLPEEDVRLYLECMRQPGHAEAGSRWYRSFQTREMLSWMRGEYDGARVHVPVRW
HHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
LSGTEDPVLTPDLLDGYAERIDDFQLELVDGVGHWIVDQRPDLVLDRVRAFLRRET
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTMPELDGVEHRYVDLGDDVTIHVADAGPASGPAVMLVHGFPQNWWEWRALIGPLAADG
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCC
YRVLCPDLRGAGWSSAPRSSYRKDEMADDLAGVLDRLGVRSVDLVAHDWGGPVAFIMMLR
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
HPEKVTGFFGVNTSAPFVKRSLSTIRNVWRFWYQIPISLPVIGPRVISTPDSRFVRLLGS
CCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
WVGGGYTLPEEDVRLYLECMRQPGHAEAGSRWYRSFQTREMLSWMRGEYDGARVHVPVRW
HHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
LSGTEDPVLTPDLLDGYAERIDDFQLELVDGVGHWIVDQRPDLVLDRVRAFLRRET
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]