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The map label for this gene is fusA2

Identifier: 41409623

GI number: 41409623

Start: 3926963

End: 3929125

Strand: Reverse

Name: fusA2

Synonym: MAP3525c

Alternate gene names: 41409623

Gene position: 3929125-3926963 (Counterclockwise)

Preceding gene: 41409624

Following gene: 41409621

Centisome position: 81.35

GC content: 70.32

Gene sequence:

>2163_bases
ATGGCGGACAAGACAACTACTTCCCAAGGCGCGGGAAACGCTCCTACCGCCAAGAGTCCGGGCGAGATTCGCAACGTCGT
GCTGGTGGGACCGTCGGGCGGCGGCAAAACCACTCTGGTCGAAGCGCTGCTGGTCGCCGCCGGGGTGTTGAACCGCCCCG
GCTCGGTCGCCGACGGCAGCACGGTCTGCGACTACGACGAGGCCGAGATCCGCCAGCAACGGTCGGTGGGGGTCGCCGTG
GCGTCCCTGTCGCACGACGGCGTCAAGGTCAACCTGGTCGACACACCCGGATACGCCGACTTCGTCGGCGAGCTGCGCGC
CGGGCTGCGGGCCGCCGACTGCGCGCTGTTCGTGATCGCGGCCAACGAGGGTGTCGACGAGCCCACCAAACTGTTGTGGC
AGGAATGCAACCAGGTGGGCATGCCCCGCGCGGTGGTGATCACCAAGCTCGACCACGCCCGCGCCAACTACGCCGAGGCG
CTGGCCGCCGCGCAGAACGCGTTCGGCGACAAGGTTTTACCGCTGTACCTGCCCACCGGTCTGCCGGCCTGCACCGGCCT
GATCGGCCTGTTGTCGCAGCAGCGCTACGCCTACGCCGACGGCAAACGCGCTGTGCGGCCGCCGGATCCCGCCGACGCCG
CGCAGATCGAGGCCGCCCGCGGCACCCTGATCGAGGGGATCATCGAGGAGTCCGAGGACGAGTCGCTGATGGAGCGGTAC
CTGGGTGGGGAGTCCATCGACGAAACGGTGCTCATCGCCGACCTGGAGCGGGCCGTCGCCCGCGGGTCGTTCTTCCCGGT
GATCCCGGTCTGCAGCAGCACCGGCGTCGGCACCCTGGAGCTGCTGGAGGTCGCCACCCGCGGCTTCCCGTCGCCGATGG
AACACCCGCTGCCGGAGGTCTTCACCCAGGTCGGCGCGCCGCGCGCCGGGCTGGCCTGCGACCCCGACGGCCCGTTGCTC
GCCGAGGTGGTGAAGACGACGTCGGACCCCTACGTGGGCAGGGTCAGCCTGGTCCGGGTGTTCTCCGGGACCATCAGGCC
CGACGCGACGGTTCACGTGTCGGGCCATTTTGCGTCGTTCTTCGGCACCGGCAACGGGAACGGCCATGCCCACCCCGATC
ACGACGAGGACGAGCGCATCGGGGTGCTGTCCTTCCCGCTGGGCAAGCAGCAACGCCCGGCCTCGGCCGTGGTGGCCGGT
GACATCTGCGCGATCGGCAAGCTGAGCCGGGCCGAAACCGGCGACACGCTGTCGGACAAGTCCGAGCCGCTGGTGCTCAA
GCCCTGGACCATGCCCGAGCCGCTGCTGCCGGTCGCGATCGCCGCACACGCCAAGACCGACGAGGACAAGCTGTCGGTGG
GGCTGGGCCGGCTGGCCGCCGAGGATCCGACGCTGCGGATCGAGCAAAACCAGGAGACCCACCAGATCGTGCTGTGGTGC
ATGGGCGAATCGCACGCCGGTGTCGTGCTGGACGCGCTGGCCAACCGGTACGGCGTCACGGTGGACACCGTGGAGCTGCG
GCTCCCGCTGCGAGAGACGTTCGCGGGCAAGGCCAAAGGGCACGGCCGGCACGTCAAACAGTCCGGCGGCCACGGCCAGT
ACGCGGTGTGCGACATCGAGGTGGAGCCGCTGCCGGAAGGCTCCGGGTTCGAGTTCGTCGACAAGGTGGTCGGCGGGGCG
GTGCCGCGGCAGTTCATTCCGAGCGTGGAGAAGGGGGTGCGCGCCCAGATGGAAAAGGGTGTGCACGCGGGCTACCCGGT
GGTCGACATTCGCGTCACCCTGCTCGACGGCAAGGCGCACAGCGTCGACTCGTCGGACTTCGCGTTCCAGATGGCGGGCG
CGCTGGCGCTGCGGGAGGCGGCCGCGGCCACCAAGGTGGTGTTGCTCGAGCCCATCGACGAGATCTCGGTGCTGGTGCCC
GACGATTTCGTCGGTGCGGTGATGGGCGATCTGTCCGGCCGGCGCGGCCGCGTGCTCGGCACCGACACCGCCGGTCACGA
GCGCACCGTGGTCAAGGCCGAGGTGCCGCAGGTGGAGCTGACCCGCTACGCCATCGACCTGCGGTCGCTGGCGCACGGCG
CGGCCTCGTTCACCCGGTCGTTCGCCCGCTACGAGCCGATGCCGGAATCCGCGGCCGCCCGAGTGGCGACCGCCGCGGTC
TGA

Upstream 100 bases:

>100_bases
GCCCGACGGTCTTGTGGTCGACGGAAGTTCGGTGAAGGGTAGCTAGAAAGGTGCTAGCCGGGCCGGCAACGGTGCGCCCC
GGGCGACCTGGAGGAAAGTC

Downstream 100 bases:

>100_bases
CCCGAATCACACTGCCGGTCAAGCGCTTTCGCCGAATTGTTCGGCCACCGCGCGGCGGTCCAGCGAGCCCTTCGCGGTGT
GCGGCAGCTCGGCGGCCCGC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MADKTTTSQGAGNAPTAKSPGEIRNVVLVGPSGGGKTTLVEALLVAAGVLNRPGSVADGSTVCDYDEAEIRQQRSVGVAV
ASLSHDGVKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKLLWQECNQVGMPRAVVITKLDHARANYAEA
LAAAQNAFGDKVLPLYLPTGLPACTGLIGLLSQQRYAYADGKRAVRPPDPADAAQIEAARGTLIEGIIEESEDESLMERY
LGGESIDETVLIADLERAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTQVGAPRAGLACDPDGPLL
AEVVKTTSDPYVGRVSLVRVFSGTIRPDATVHVSGHFASFFGTGNGNGHAHPDHDEDERIGVLSFPLGKQQRPASAVVAG
DICAIGKLSRAETGDTLSDKSEPLVLKPWTMPEPLLPVAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQIVLWC
MGESHAGVVLDALANRYGVTVDTVELRLPLRETFAGKAKGHGRHVKQSGGHGQYAVCDIEVEPLPEGSGFEFVDKVVGGA
VPRQFIPSVEKGVRAQMEKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVVLLEPIDEISVLVP
DDFVGAVMGDLSGRRGRVLGTDTAGHERTVVKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVATAAV

Sequences:

>Translated_720_residues
MADKTTTSQGAGNAPTAKSPGEIRNVVLVGPSGGGKTTLVEALLVAAGVLNRPGSVADGSTVCDYDEAEIRQQRSVGVAV
ASLSHDGVKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKLLWQECNQVGMPRAVVITKLDHARANYAEA
LAAAQNAFGDKVLPLYLPTGLPACTGLIGLLSQQRYAYADGKRAVRPPDPADAAQIEAARGTLIEGIIEESEDESLMERY
LGGESIDETVLIADLERAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTQVGAPRAGLACDPDGPLL
AEVVKTTSDPYVGRVSLVRVFSGTIRPDATVHVSGHFASFFGTGNGNGHAHPDHDEDERIGVLSFPLGKQQRPASAVVAG
DICAIGKLSRAETGDTLSDKSEPLVLKPWTMPEPLLPVAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQIVLWC
MGESHAGVVLDALANRYGVTVDTVELRLPLRETFAGKAKGHGRHVKQSGGHGQYAVCDIEVEPLPEGSGFEFVDKVVGGA
VPRQFIPSVEKGVRAQMEKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVVLLEPIDEISVLVP
DDFVGAVMGDLSGRRGRVLGTDTAGHERTVVKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVATAAV
>Mature_719_residues
ADKTTTSQGAGNAPTAKSPGEIRNVVLVGPSGGGKTTLVEALLVAAGVLNRPGSVADGSTVCDYDEAEIRQQRSVGVAVA
SLSHDGVKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKLLWQECNQVGMPRAVVITKLDHARANYAEAL
AAAQNAFGDKVLPLYLPTGLPACTGLIGLLSQQRYAYADGKRAVRPPDPADAAQIEAARGTLIEGIIEESEDESLMERYL
GGESIDETVLIADLERAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTQVGAPRAGLACDPDGPLLA
EVVKTTSDPYVGRVSLVRVFSGTIRPDATVHVSGHFASFFGTGNGNGHAHPDHDEDERIGVLSFPLGKQQRPASAVVAGD
ICAIGKLSRAETGDTLSDKSEPLVLKPWTMPEPLLPVAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQIVLWCM
GESHAGVVLDALANRYGVTVDTVELRLPLRETFAGKAKGHGRHVKQSGGHGQYAVCDIEVEPLPEGSGFEFVDKVVGGAV
PRQFIPSVEKGVRAQMEKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVVLLEPIDEISVLVPD
DFVGAVMGDLSGRRGRVLGTDTAGHERTVVKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVATAAV

Specific function: This Protein Promotes The GTP-Dependent Translocation Of The Nascent Protein Chain From The A-Site To The P-Site Of The Ribosome. [C]

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=707, Percent_Identity=29.1371994342291, Blast_Score=259, Evalue=7e-69,
Organism=Homo sapiens, GI19923640, Length=743, Percent_Identity=26.1103633916555, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI25306283, Length=424, Percent_Identity=28.3018867924528, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI25306287, Length=359, Percent_Identity=24.5125348189415, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1789738, Length=717, Percent_Identity=33.0543933054393, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1790835, Length=289, Percent_Identity=24.5674740484429, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI48994988, Length=130, Percent_Identity=28.4615384615385, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=716, Percent_Identity=26.8156424581006, Blast_Score=240, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17556745, Length=201, Percent_Identity=28.8557213930348, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=699, Percent_Identity=25.3218884120172, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6322359, Length=808, Percent_Identity=23.6386138613861, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24582462, Length=700, Percent_Identity=30, Blast_Score=269, Evalue=5e-72,
Organism=Drosophila melanogaster, GI221458488, Length=749, Percent_Identity=24.0320427236315, Blast_Score=172, Evalue=1e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75837; Mature: 75705

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKTTTSQGAGNAPTAKSPGEIRNVVLVGPSGGGKTTLVEALLVAAGVLNRPGSVADGS
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TVCDYDEAEIRQQRSVGVAVASLSHDGVKVNLVDTPGYADFVGELRAGLRAADCALFVIA
EECCCCHHHHHHHHHHCEEEEEECCCCEEEEEEECCCHHHHHHHHHHCCEECCEEEEEEE
ANEGVDEPTKLLWQECNQVGMPRAVVITKLDHARANYAEALAAAQNAFGDKVLPLYLPTG
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
LPACTGLIGLLSQQRYAYADGKRAVRPPDPADAAQIEAARGTLIEGIIEESEDESLMERY
CHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGGESIDETVLIADLERAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEV
CCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
FTQVGAPRAGLACDPDGPLLAEVVKTTSDPYVGRVSLVRVFSGTIRPDATVHVSGHFASF
HHHHCCCCCCCEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECCEEEE
FGTGNGNGHAHPDHDEDERIGVLSFPLGKQQRPASAVVAGDICAIGKLSRAETGDTLSDK
EECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCC
SEPLVLKPWTMPEPLLPVAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQIVLWC
CCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEE
MGESHAGVVLDALANRYGVTVDTVELRLPLRETFAGKAKGHGRHVKQSGGHGQYAVCDIE
ECCCCCCHHHHHHHHHHCCEEEEEEEEECHHHHHCCCCCCCCCCHHCCCCCCCEEEEEEE
VEPLPEGSGFEFVDKVVGGAVPRQFIPSVEKGVRAQMEKGVHAGYPVVDIRVTLLDGKAH
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
SVDSSDFAFQMAGALALREAAAATKVVLLEPIDEISVLVPDDFVGAVMGDLSGRRGRVLG
CCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCHHHHHHHHHCCCCCCCEEE
TDTAGHERTVVKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVATAAV
CCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ADKTTTSQGAGNAPTAKSPGEIRNVVLVGPSGGGKTTLVEALLVAAGVLNRPGSVADGS
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TVCDYDEAEIRQQRSVGVAVASLSHDGVKVNLVDTPGYADFVGELRAGLRAADCALFVIA
EECCCCHHHHHHHHHHCEEEEEECCCCEEEEEEECCCHHHHHHHHHHCCEECCEEEEEEE
ANEGVDEPTKLLWQECNQVGMPRAVVITKLDHARANYAEALAAAQNAFGDKVLPLYLPTG
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
LPACTGLIGLLSQQRYAYADGKRAVRPPDPADAAQIEAARGTLIEGIIEESEDESLMERY
CHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGGESIDETVLIADLERAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEV
CCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
FTQVGAPRAGLACDPDGPLLAEVVKTTSDPYVGRVSLVRVFSGTIRPDATVHVSGHFASF
HHHHCCCCCCCEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECCEEEE
FGTGNGNGHAHPDHDEDERIGVLSFPLGKQQRPASAVVAGDICAIGKLSRAETGDTLSDK
EECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCC
SEPLVLKPWTMPEPLLPVAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQIVLWC
CCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEE
MGESHAGVVLDALANRYGVTVDTVELRLPLRETFAGKAKGHGRHVKQSGGHGQYAVCDIE
ECCCCCCHHHHHHHHHHCCEEEEEEEEECHHHHHCCCCCCCCCCHHCCCCCCCEEEEEEE
VEPLPEGSGFEFVDKVVGGAVPRQFIPSVEKGVRAQMEKGVHAGYPVVDIRVTLLDGKAH
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
SVDSSDFAFQMAGALALREAAAATKVVLLEPIDEISVLVPDDFVGAVMGDLSGRRGRVLG
CCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCHHHHHHHHHCCCCCCCEEE
TDTAGHERTVVKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVATAAV
CCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]