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The map label for this gene is folD

Identifier: 41409561

GI number: 41409561

Start: 3844745

End: 3845590

Strand: Reverse

Name: folD

Synonym: MAP3463c

Alternate gene names: 41409561

Gene position: 3845590-3844745 (Counterclockwise)

Preceding gene: 41409565

Following gene: 41409560

Centisome position: 79.62

GC content: 70.45

Gene sequence:

>846_bases
GTGGGAGCAATCACACTGGACGGCAAGGCCACCCGCGACGAAATCTTCGTCGACCTCAAGCAGCGGGTCGCCGCGTTGAC
CGCGGCCGGCCGCACCCCCGGTTTGGGCACCATCCTGGTCGGCGACGACCCGGGCTCGCAGGCCTACGTGCGCGGCAAGC
ACGCCGACTGCGCCAAGGTGGGCATCACCTCGATCCGCCGCGACCTGCCCGCCGACATCAGCACCGCCGCGCTCAACGAC
ACCATCGACGAGCTGAACGCCAACCCCGAATGCACGGGGTACATCGTGCAGTTGCCGCTGCCCAAGCAGTTGGACGAGAA
CGCGGCGCTGGAGCGGGTGGACCCGGACAAGGACGCCGACGGCCTGCACCCCACCAACCTGGGCCGGTTGGTGCTCAACA
ATCCGGCGCCGCTGCCGTGCACGCCGCGCGGAATCGTGCACCTGTTGCGCCGCTACGACGCCGAGATCGCCGGGGCGCAC
GTGGTGGTCATCGGCCGCGGTGTGACGGTCGGTCGTCCGCTGGGGCTGCTGCTGACCCGGCGTTCGGAGAACGCGACGGT
TACGTTGTGCCACACCGGAACTCGTGATCTGCCGGCGTTGACCAGGCAGGCCGACATCATCGTGGCCGCCGTCGGGGTTC
CGCACCTGCTGACCGCCGACATGGTGCGCCCGGGGGCCGCGGTGCTCGACGTGGGGGTCAGCCGGGTGGACGGCAAGCTC
GCCGGCGACGTGCACCCCGACGTGTGGGAGGTGGCCGGTCACGTGTCGCCGAATCCCGGCGGCGTGGGCCCGTTGACCCG
GGCGTTCCTGTTGACCAACGTCGTCGAGCTGGCCGAGCGGGAATGA

Upstream 100 bases:

>100_bases
GACTTGATGATGCGATTGCGCAGCGTGATCGGACCGAGCTTGGCGGGGCTGAACACGTCCGGAGGGGTGGGCATATCGGG
GAGCGTGCCAGACTGTCAGC

Downstream 100 bases:

>100_bases
CCGCACGCACCCTGATGCGGGCCCAGTGGCCGATTCTGTTGGTGGGCTTGATCTTTGCGGTGGCACTGGCCTTGGTGGGC
GCCAACTTCTGGCGCCGCGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MGAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKVGITSIRRDLPADISTAALND
TIDELNANPECTGYIVQLPLPKQLDENAALERVDPDKDADGLHPTNLGRLVLNNPAPLPCTPRGIVHLLRRYDAEIAGAH
VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLPALTRQADIIVAAVGVPHLLTADMVRPGAAVLDVGVSRVDGKL
AGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE

Sequences:

>Translated_281_residues
MGAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKVGITSIRRDLPADISTAALND
TIDELNANPECTGYIVQLPLPKQLDENAALERVDPDKDADGLHPTNLGRLVLNNPAPLPCTPRGIVHLLRRYDAEIAGAH
VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLPALTRQADIIVAAVGVPHLLTADMVRPGAAVLDVGVSRVDGKL
AGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE
>Mature_280_residues
GAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKVGITSIRRDLPADISTAALNDT
IDELNANPECTGYIVQLPLPKQLDENAALERVDPDKDADGLHPTNLGRLVLNNPAPLPCTPRGIVHLLRRYDAEIAGAHV
VVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLPALTRQADIIVAAVGVPHLLTADMVRPGAAVLDVGVSRVDGKLA
GDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=39.7959183673469, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI94721354, Length=302, Percent_Identity=40.3973509933775, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=35.2941176470588, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=28.0952380952381, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=44.0860215053763, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17568735, Length=258, Percent_Identity=36.046511627907, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319558, Length=259, Percent_Identity=39.7683397683398, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=36.2126245847176, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=41.6949152542373, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=41.6949152542373, Blast_Score=215, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=41.0958904109589, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=41.0958904109589, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=41.0958904109589, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=41.0958904109589, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29539; Mature: 29408

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKV
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCHHHH
GITSIRRDLPADISTAALNDTIDELNANPECTGYIVQLPLPKQLDENAALERVDPDKDAD
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCC
GLHPTNLGRLVLNNPAPLPCTPRGIVHLLRRYDAEIAGAHVVVIGRGVTVGRPLGLLLTR
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCEEEEEE
RSENATVTLCHTGTRDLPALTRQADIIVAAVGVPHLLTADMVRPGAAVLDVGVSRVDGKL
CCCCCEEEEEECCCCCCHHHHCCCCEEEEECCCCHHHHHHHCCCCHHEEHHCHHHCCCEE
AGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKV
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCHHHH
GITSIRRDLPADISTAALNDTIDELNANPECTGYIVQLPLPKQLDENAALERVDPDKDAD
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCC
GLHPTNLGRLVLNNPAPLPCTPRGIVHLLRRYDAEIAGAHVVVIGRGVTVGRPLGLLLTR
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCEEEEEE
RSENATVTLCHTGTRDLPALTRQADIIVAAVGVPHLLTADMVRPGAAVLDVGVSRVDGKL
CCCCCEEEEEECCCCCCHHHHCCCCEEEEECCCCHHHHHHHCCCCHHEEHHCHHHCCCEE
AGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA