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The map label for this gene is dacB [H]

Identifier: 41409546

GI number: 41409546

Start: 3827806

End: 3828990

Strand: Direct

Name: dacB [H]

Synonym: MAP3448

Alternate gene names: 41409546

Gene position: 3827806-3828990 (Clockwise)

Preceding gene: 41409545

Following gene: 41409547

Centisome position: 79.25

GC content: 72.91

Gene sequence:

>1185_bases
GTGTCGGGCACGGCGTCGCCGCTGGGACGGGCCGAGCCCGGCGCCGAACCCAATGCCGCGCCGGCGAACTGCCCGTACAA
GGTGAACACCCCGCCGGCCGTGGATTCCTCGGAGGTCCCCACCGCCGGTGACCCGCCGATGCCGCTGGCGGTGCCCGCCA
AGCCGGTCGGCGGCGACGCCCTGGGCAGCTGCGGCATCGTCGCCGCGCCCAACACGCCGCCGCTGCCCGGCGACGTGTCC
GCCGAGGCCTGGCTGGTGGCCGACCTGGACAGCGGCGCGGTCATCGCCGCCAAGGACCCGCACGGCCGGCACCGCCCGGC
CAGCATCATCAAGGTGCTGGTCGCGATGGCCGCCATCAACACGCTCAACCTGAACAAGGCGGTGCCCGGCACCGCCGACG
ACGCGGCCGCCGAGGGCACCAAGGTGGGCGTCGAGGAGGGCGGCGTGTTCACCGTCAACCAGCTTCTGCACGGGCTGCTG
ATGCACTCCGGCAACGACGCCGCGCACGCGCTGGCCATGCAGCTGGGCGGCATGCAGCAGGCGCTGGAGAAGATCAACGT
GCTGGCAGCCAAGCTGGGCGGGCAGGACACCCGGGTGGCCACACCGTCGGGTCTGGACGCGCCGGGCATGAGCACCTCGG
CCTACGACATCGGGCTGTTCTACCGCTACGCCTGGCAGAACCCGACCTTCGCCGACATCGTCGCGACCCGCACCTTCGAC
TTCCCCGGCCACGGCGACCACCCGGGCTACGAGCTGGAGAACGACAACCAGCTGCTCTACAAGTATCCGGGCGCGCTGGG
CGGCAAGACCGGCTACACCGACGACGCGGGCCAGACGTTCGTGGGCGCGGCCAATCACGACGGGCGACGGCTGGTGGCGG
TGCTGCTGCACGGGACGCGGCAGCCGATCGCGCCCTGGGAGCAGGCCGCGCACCTGCTGGACTACGGGTTCGCCACGCCG
CAGGGCACCCAGGTCGGCTCGCTGATCGCGCCCGACCCGTCGCTGGCGCCGGCCAAGCGCGACGGCGCCGCCGACCGGCT
GGCCGGCAACGCCGCGGCCGCCGGGATGATCTCCTCGGCCGACGCGCTCCCGGTCCGGGTGGGGGTGGCGGTGATCGGCA
CCATCGTGGTGTTCACACTGATCATGATGGCCCGCTCGATGAACCGCCGACCCCAGCACAGCTGA

Upstream 100 bases:

>100_bases
CCCGATCGCTTACTGTGCACAGACGATGTCAACTTCAGGGACCCTGGTACGTTCCGCATCATGCCTGGCAGCGGCGCTTT
TCCTGGCGGCCGCGCCGGCG

Downstream 100 bases:

>100_bases
GCACCGTGGCACGCGGATCGCGGCGCGGCCTGCTGGTCGGGCTGACCGCCGCCAGCGTGGGCGTCATCTACGGTTACGAC
CTGTCCATCATCGCCGGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSGTASPLGRAEPGAEPNAAPANCPYKVNTPPAVDSSEVPTAGDPPMPLAVPAKPVGGDALGSCGIVAAPNTPPLPGDVS
AEAWLVADLDSGAVIAAKDPHGRHRPASIIKVLVAMAAINTLNLNKAVPGTADDAAAEGTKVGVEEGGVFTVNQLLHGLL
MHSGNDAAHALAMQLGGMQQALEKINVLAAKLGGQDTRVATPSGLDAPGMSTSAYDIGLFYRYAWQNPTFADIVATRTFD
FPGHGDHPGYELENDNQLLYKYPGALGGKTGYTDDAGQTFVGAANHDGRRLVAVLLHGTRQPIAPWEQAAHLLDYGFATP
QGTQVGSLIAPDPSLAPAKRDGAADRLAGNAAAAGMISSADALPVRVGVAVIGTIVVFTLIMMARSMNRRPQHS

Sequences:

>Translated_394_residues
MSGTASPLGRAEPGAEPNAAPANCPYKVNTPPAVDSSEVPTAGDPPMPLAVPAKPVGGDALGSCGIVAAPNTPPLPGDVS
AEAWLVADLDSGAVIAAKDPHGRHRPASIIKVLVAMAAINTLNLNKAVPGTADDAAAEGTKVGVEEGGVFTVNQLLHGLL
MHSGNDAAHALAMQLGGMQQALEKINVLAAKLGGQDTRVATPSGLDAPGMSTSAYDIGLFYRYAWQNPTFADIVATRTFD
FPGHGDHPGYELENDNQLLYKYPGALGGKTGYTDDAGQTFVGAANHDGRRLVAVLLHGTRQPIAPWEQAAHLLDYGFATP
QGTQVGSLIAPDPSLAPAKRDGAADRLAGNAAAAGMISSADALPVRVGVAVIGTIVVFTLIMMARSMNRRPQHS
>Mature_393_residues
SGTASPLGRAEPGAEPNAAPANCPYKVNTPPAVDSSEVPTAGDPPMPLAVPAKPVGGDALGSCGIVAAPNTPPLPGDVSA
EAWLVADLDSGAVIAAKDPHGRHRPASIIKVLVAMAAINTLNLNKAVPGTADDAAAEGTKVGVEEGGVFTVNQLLHGLLM
HSGNDAAHALAMQLGGMQQALEKINVLAAKLGGQDTRVATPSGLDAPGMSTSAYDIGLFYRYAWQNPTFADIVATRTFDF
PGHGDHPGYELENDNQLLYKYPGALGGKTGYTDDAGQTFVGAANHDGRRLVAVLLHGTRQPIAPWEQAAHLLDYGFATPQ
GTQVGSLIAPDPSLAPAKRDGAADRLAGNAAAAGMISSADALPVRVGVAVIGTIVVFTLIMMARSMNRRPQHS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=284, Percent_Identity=32.0422535211268, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786851, Length=278, Percent_Identity=29.136690647482, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787062, Length=265, Percent_Identity=30.188679245283, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 40382; Mature: 40251

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTASPLGRAEPGAEPNAAPANCPYKVNTPPAVDSSEVPTAGDPPMPLAVPAKPVGGDA
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
LGSCGIVAAPNTPPLPGDVSAEAWLVADLDSGAVIAAKDPHGRHRPASIIKVLVAMAAIN
CCCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
TLNLNKAVPGTADDAAAEGTKVGVEEGGVFTVNQLLHGLLMHSGNDAAHALAMQLGGMQQ
HCCCCCCCCCCCCHHHCCCCEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCHHH
ALEKINVLAAKLGGQDTRVATPSGLDAPGMSTSAYDIGLFYRYAWQNPTFADIVATRTFD
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHEEEEEEC
FPGHGDHPGYELENDNQLLYKYPGALGGKTGYTDDAGQTFVGAANHDGRRLVAVLLHGTR
CCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEHHCCC
QPIAPWEQAAHLLDYGFATPQGTQVGSLIAPDPSLAPAKRDGAADRLAGNAAAAGMISSA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHCCCHHHHHHHCCC
DALPVRVGVAVIGTIVVFTLIMMARSMNRRPQHS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGTASPLGRAEPGAEPNAAPANCPYKVNTPPAVDSSEVPTAGDPPMPLAVPAKPVGGDA
CCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
LGSCGIVAAPNTPPLPGDVSAEAWLVADLDSGAVIAAKDPHGRHRPASIIKVLVAMAAIN
CCCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
TLNLNKAVPGTADDAAAEGTKVGVEEGGVFTVNQLLHGLLMHSGNDAAHALAMQLGGMQQ
HCCCCCCCCCCCCHHHCCCCEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCHHH
ALEKINVLAAKLGGQDTRVATPSGLDAPGMSTSAYDIGLFYRYAWQNPTFADIVATRTFD
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHEEEEEEC
FPGHGDHPGYELENDNQLLYKYPGALGGKTGYTDDAGQTFVGAANHDGRRLVAVLLHGTR
CCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEHHCCC
QPIAPWEQAAHLLDYGFATPQGTQVGSLIAPDPSLAPAKRDGAADRLAGNAAAAGMISSA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHCCCHHHHHHHCCC
DALPVRVGVAVIGTIVVFTLIMMARSMNRRPQHS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]