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The map label for this gene is mdlC [H]

Identifier: 41409098

GI number: 41409098

Start: 3336694

End: 3338403

Strand: Reverse

Name: mdlC [H]

Synonym: MAP3000c

Alternate gene names: 41409098

Gene position: 3338403-3336694 (Counterclockwise)

Preceding gene: 41409100

Following gene: 41409096

Centisome position: 69.12

GC content: 73.68

Gene sequence:

>1710_bases
ATGACGCTAGACAATCCACTCGACGGACCCGAGGCGGCGGCGGACCTGATCGCGCTCCTGGCCGACGAGGGAGTCGCGGA
CTTCTTCATCAACCCCGGAACCGACTCGGCGCCCATCCAGGAAGCGCTCGCGGCGGCGCGCGCCGCCGGTACGCCCAGCC
CGCGGGCGGTGCTGTGCGTGCACGAAAGCGTCGCCCTGGCGGCGGCCATCGGCCACCACATGGCCAGCGCCCGGCCGCAG
GCGGTCATGGTGCACGTCGACGCCGGGACGCTGAACCTGGGCTGCCAGCTGCACAACGCGCAGCGCAACGGAACCCCGGT
GGTGGTATTCGCCGGGCGCACCCCCTACAGCTCGGCGCCCGACGTCCGCGGCCACCGCGACACCCACATCCATTGGCAGC
AGGAACAACTCGACCAGCCCGCCGTGATGCGCAATTACGCCAAGTGGCACATGGAGGTTCCGCGCGGGCGTGAGCTGGCC
CCCATCGTCCGCCGGGCATTCCAGGTCGCCCAGTCCAGCCCGCCCGGACCGGCCTACGTCATGCTGCCGCGCGAAGCCCT
GATGGAGCCCGGCGGCCGGGCGTTGCAGCGACGGCTGCCGCCGGCCGTTCCGCCCGGCCCCGACCCCGCCGCGCTGGGCC
GGCTGGCCGGGATCCTGGTCGCCGGGCAGCGGGTGGTCATCGTCACCGCCCGCACCGCCGCCGACCCGGCCGCCGCGACC
GTGCTGGCTCGCATCGCCGAGATCCTCGGAGCGCCGGTGATCGACCAGGGCGACCGCGCCAACCTGCCGCCCGGGCATCC
GCTGCACGTCACCGGCGACGCGGCCCCGCTGCAGGACGCCGACACGGTGCTGCTGCTGGACAGCGAAGTGCCGTGGGTGC
CCGCACAGTTGGCCCCGCCTGCGACGGCCCGGGTGGTGCAGATCGACGCCGACCCGGTCAAGCCGAGCATGCCGCTGTGG
TCCTACCCGGTCGAAATCGCGTTGACCGCCGACACCCGGGTCGCGCTGCTGCTGCTCGAGCAGACCCTGCTGCGGCTGGC
CAACGACGAGCTGCGCGAAAAGTGGAGCACCCGCCGCGGCGCGGTGGAATCCCAAGTGGCGCAACGCCGCCGGGAAGCGG
CGCGCCGGGCCGAGTCCGACAGCGACGCCGATGCCCCCGACGCGATGCTGGCCGCGCTCGGCGGCGCGCTGCCCGACGAC
GCCGTCGTCGTCCAGGAGGCCGTGACCAACCGGCCCGCGGTGGCCCGCCAGGTGCGGCGGCCGCCCGGGCAGCTCTTCGA
CACCGGCGCACCGGCTTTGGGCTGGGCGCTGGGCGGCGCCTTCGGGATCAAACTGGCCCGGCCGCACGCACCCGTCGTCG
CGATCTGCGGCGACGGGTCGTTCAACTTCGGGGTGCCCACCGCCGCGCTGTGGTCCGCGCATCGCTACGGCGCGCCGTTT
GTGACCGTCGTGTTGAACAACCACTCCTACCTGGCCTCCAAGACGCCGGTGACGGAGCTGTACCCGCAGGGAATATCCGT
GCGCGACAACGACTTTCCCGAAACTCGGCTGACCCCGGAGACCGACTACGCCGCGCTGGCCCGGGCCTGCGGGGGCAGCG
GACGCGACGTGCACACCCCGGCGCAGATGCGCGAGGCCATCGGGTGGGCGCTGGACGAGGCGGGCCAGGGCCGGTGCGCG
GTGCTCGACGTGCGGCTGCCGCAAACCTAG

Upstream 100 bases:

>100_bases
AAAGGATCAACAACGCCGACGCGGAACGTATCTTGAAGATCTCATCCGAGACACGAGGGGGATTTGCGAGGTCGGCGACG
TCGATAGGAGAACGGCAGCC

Downstream 100 bases:

>100_bases
GGACGCCCGGCCCGGGACGCGGCCCGGCACGGCTGAAGCACTCAGTGCCGATGCTCGGCGGGGTCGGGGGAGCCGCCCAT
CATCCGCACCATCGCCAGCC

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MTLDNPLDGPEAAADLIALLADEGVADFFINPGTDSAPIQEALAAARAAGTPSPRAVLCVHESVALAAAIGHHMASARPQ
AVMVHVDAGTLNLGCQLHNAQRNGTPVVVFAGRTPYSSAPDVRGHRDTHIHWQQEQLDQPAVMRNYAKWHMEVPRGRELA
PIVRRAFQVAQSSPPGPAYVMLPREALMEPGGRALQRRLPPAVPPGPDPAALGRLAGILVAGQRVVIVTARTAADPAAAT
VLARIAEILGAPVIDQGDRANLPPGHPLHVTGDAAPLQDADTVLLLDSEVPWVPAQLAPPATARVVQIDADPVKPSMPLW
SYPVEIALTADTRVALLLLEQTLLRLANDELREKWSTRRGAVESQVAQRRREAARRAESDSDADAPDAMLAALGGALPDD
AVVVQEAVTNRPAVARQVRRPPGQLFDTGAPALGWALGGAFGIKLARPHAPVVAICGDGSFNFGVPTAALWSAHRYGAPF
VTVVLNNHSYLASKTPVTELYPQGISVRDNDFPETRLTPETDYAALARACGGSGRDVHTPAQMREAIGWALDEAGQGRCA
VLDVRLPQT

Sequences:

>Translated_569_residues
MTLDNPLDGPEAAADLIALLADEGVADFFINPGTDSAPIQEALAAARAAGTPSPRAVLCVHESVALAAAIGHHMASARPQ
AVMVHVDAGTLNLGCQLHNAQRNGTPVVVFAGRTPYSSAPDVRGHRDTHIHWQQEQLDQPAVMRNYAKWHMEVPRGRELA
PIVRRAFQVAQSSPPGPAYVMLPREALMEPGGRALQRRLPPAVPPGPDPAALGRLAGILVAGQRVVIVTARTAADPAAAT
VLARIAEILGAPVIDQGDRANLPPGHPLHVTGDAAPLQDADTVLLLDSEVPWVPAQLAPPATARVVQIDADPVKPSMPLW
SYPVEIALTADTRVALLLLEQTLLRLANDELREKWSTRRGAVESQVAQRRREAARRAESDSDADAPDAMLAALGGALPDD
AVVVQEAVTNRPAVARQVRRPPGQLFDTGAPALGWALGGAFGIKLARPHAPVVAICGDGSFNFGVPTAALWSAHRYGAPF
VTVVLNNHSYLASKTPVTELYPQGISVRDNDFPETRLTPETDYAALARACGGSGRDVHTPAQMREAIGWALDEAGQGRCA
VLDVRLPQT
>Mature_568_residues
TLDNPLDGPEAAADLIALLADEGVADFFINPGTDSAPIQEALAAARAAGTPSPRAVLCVHESVALAAAIGHHMASARPQA
VMVHVDAGTLNLGCQLHNAQRNGTPVVVFAGRTPYSSAPDVRGHRDTHIHWQQEQLDQPAVMRNYAKWHMEVPRGRELAP
IVRRAFQVAQSSPPGPAYVMLPREALMEPGGRALQRRLPPAVPPGPDPAALGRLAGILVAGQRVVIVTARTAADPAAATV
LARIAEILGAPVIDQGDRANLPPGHPLHVTGDAAPLQDADTVLLLDSEVPWVPAQLAPPATARVVQIDADPVKPSMPLWS
YPVEIALTADTRVALLLLEQTLLRLANDELREKWSTRRGAVESQVAQRRREAARRAESDSDADAPDAMLAALGGALPDDA
VVVQEAVTNRPAVARQVRRPPGQLFDTGAPALGWALGGAFGIKLARPHAPVVAICGDGSFNFGVPTAALWSAHRYGAPFV
TVVLNNHSYLASKTPVTELYPQGISVRDNDFPETRLTPETDYAALARACGGSGRDVHTPAQMREAIGWALDEAGQGRCAV
LDVRLPQT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=24.2160278745645, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790104, Length=520, Percent_Identity=22.8846153846154, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788716, Length=519, Percent_Identity=23.5067437379576, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17531299, Length=575, Percent_Identity=25.7391304347826, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=575, Percent_Identity=25.7391304347826, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922626, Length=503, Percent_Identity=24.2544731610338, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 60223; Mature: 60092

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDNPLDGPEAAADLIALLADEGVADFFINPGTDSAPIQEALAAARAAGTPSPRAVLCV
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
HESVALAAAIGHHMASARPQAVMVHVDAGTLNLGCQLHNAQRNGTPVVVFAGRTPYSSAP
EHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECEEEECCCCCCCEEEEEECCCCCCCCC
DVRGHRDTHIHWQQEQLDQPAVMRNYAKWHMEVPRGRELAPIVRRAFQVAQSSPPGPAYV
CCCCCCCCEEEECHHHCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCEEE
MLPREALMEPGGRALQRRLPPAVPPGPDPAALGRLAGILVAGQRVVIVTARTAADPAAAT
EECHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
VLARIAEILGAPVIDQGDRANLPPGHPLHVTGDAAPLQDADTVLLLDSEVPWVPAQLAPP
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCC
ATARVVQIDADPVKPSMPLWSYPVEIALTADTRVALLLLEQTLLRLANDELREKWSTRRG
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AVESQVAQRRREAARRAESDSDADAPDAMLAALGGALPDDAVVVQEAVTNRPAVARQVRR
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCC
PPGQLFDTGAPALGWALGGAFGIKLARPHAPVVAICGDGSFNFGVPTAALWSAHRYGAPF
CCCHHHCCCCCHHHHHHCCHHEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCE
VTVVLNNHSYLASKTPVTELYPQGISVRDNDFPETRLTPETDYAALARACGGSGRDVHTP
EEEEEECCCEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCH
AQMREAIGWALDEAGQGRCAVLDVRLPQT
HHHHHHHHHHHHCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TLDNPLDGPEAAADLIALLADEGVADFFINPGTDSAPIQEALAAARAAGTPSPRAVLCV
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
HESVALAAAIGHHMASARPQAVMVHVDAGTLNLGCQLHNAQRNGTPVVVFAGRTPYSSAP
EHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECEEEECCCCCCCEEEEEECCCCCCCCC
DVRGHRDTHIHWQQEQLDQPAVMRNYAKWHMEVPRGRELAPIVRRAFQVAQSSPPGPAYV
CCCCCCCCEEEECHHHCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCEEE
MLPREALMEPGGRALQRRLPPAVPPGPDPAALGRLAGILVAGQRVVIVTARTAADPAAAT
EECHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
VLARIAEILGAPVIDQGDRANLPPGHPLHVTGDAAPLQDADTVLLLDSEVPWVPAQLAPP
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCC
ATARVVQIDADPVKPSMPLWSYPVEIALTADTRVALLLLEQTLLRLANDELREKWSTRRG
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AVESQVAQRRREAARRAESDSDADAPDAMLAALGGALPDDAVVVQEAVTNRPAVARQVRR
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCC
PPGQLFDTGAPALGWALGGAFGIKLARPHAPVVAICGDGSFNFGVPTAALWSAHRYGAPF
CCCHHHCCCCCHHHHHHCCHHEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCE
VTVVLNNHSYLASKTPVTELYPQGISVRDNDFPETRLTPETDYAALARACGGSGRDVHTP
EEEEEECCCEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCH
AQMREAIGWALDEAGQGRCAVLDVRLPQT
HHHHHHHHHHHHCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]