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The map label for this gene is efpA_2

Identifier: 41409013

GI number: 41409013

Start: 3246435

End: 3248051

Strand: Reverse

Name: efpA_2

Synonym: MAP2915c

Alternate gene names: 41409013

Gene position: 3248051-3246435 (Counterclockwise)

Preceding gene: 41409014

Following gene: 41409012

Centisome position: 67.25

GC content: 69.45

Gene sequence:

>1617_bases
ATGACGGCTCTCAACGACTCAGAGCGCGCGGTCCAGAACTGGACATCTGCACGCCCCGATCGTCCGGCCCCGGTGCGCAG
CACGCCGCCGGCGGAGACCGCCCCCAAGCCCGCGGCGGAGACCGCCGTAAAGCGGACGAGCAAGTACTACCCGGCGTGGC
TGCCGTCGCGCCGCTTCATCGCCGCCGTCATCGCCATCGGCGGCATGCAGCTGTTGGCCACGATGGACAGCACCGTGGCG
ATCGTCGCGCTGCCCCGGATCCAGAACGAGCTCAGTCTCTCCGACGCCGGCCGCAGCTGGGTGATCACCGCCTACGTGCT
GACGTTCGGCGGGCTGATGCTGCTCGGCGGCCGGCTCGGCGACACCATCGGGCGCAAACGCACCTTCATCGTCGGCGTCG
CGCTGTTCACCATCTCCTCGGTGCTGTGCGCGGTCGCCTGGGACGAGGCGACCATGGTGATCGCCCGGCTCTCGCAGGGG
GTCGGGTCGGCCATCGCCTCACCGACGGGCCTGGCGCTGGTCGCCACCACCTTCCCCAAGGGGCCCGCCCGTAACTTCGC
GACGGCGGTGTTCGCCGCGATGACGGCGGTCGGCTCGGTGATGGGCCTGGTGGTCGGCGGGGCGCTGACCGAGGTGTCCT
GGCGGCTGGCGTTTTTGGTGAACGTGCCGATCGGGCTGGTGATGATGTACCTGGCCCGCACCGCGCTGCGCGAGACCAAC
CGCGAGCGGATGAAACTGGACGCCACCGGCGCCGTGCTGGCCACCCTGGCCTGCACCGCCGCCGTGTTCGCCTTCTCGAT
GGGCCCGGAGAAGGGCTGGATCTCGCTGACCACCATCAGCTCCGGTGTGGTCGCGCTGGGCGCCGCCCTGGCCTTCGTCA
TCGTGGAGCGCACGGCGGAAAACCCGGTGGTGCCGTTCGACCTGTTCCGCGACCGCAACCGGCTGGTCACCTTCATCGCG
ATCTTCTTGGCCGGCGGCGTCATGTTCACCCTGACCGTGTGCATCGGCCTGTACGTGCAGGACATCCTCGGCTACAGCGC
GCTGCGCGCCGGGGTGGGCTTCATCCCGTTCGTCATCGCGATGGGCATCGGTCTGGGCGTCTCCTCGCAACTGGTGGCGC
GGTTCTCGCCGCGGGTGCTGACTATCGCCGGCGGCTACCTGCTGGTGCTCGCCATGCTCTACGGCTGGTGGTGCATGCAC
CGCGGCGTTCCCTATTTCCCGAACCTGGTACTGCCCATCGTGGTGGGCGGCATCGGCATCGGCATGGCCGTCGTCCCGCT
GACCCTGTCGGCCATCGCCGGCGTGGGTTTCGACCAGATCGGCCCGGTATCCGCGGTCACGCTGATGCTGCAGAGCCTGG
GCGGACCGCTGGTGCTGGCCGTCATCCAGGCCGTGATCACCTCGCGCACACTGTATCTGGGCGGCACCACCGGCCCGGTG
AAGACCATGAACGACGCCCAGTTGCAGGCGCTCGACCACGGCTACACCTACGGGCTGCTGTGGGTCGCCGGGGCGGCCGT
CATCGTGGGTGCCGCGGCGCTGTTCATCGGCTACACACCCGAGCAGGTCGCGCACGCCCAGGAGGTCAAGGAAGCGATGG
ACGCCGGGGAGCTCTGA

Upstream 100 bases:

>100_bases
TCGTGCCGAAGGTATCCGACCTCCCGCGATCATCGTGATCGGCGCGGTGGCGGCTTTCGGGGTTTAAACGGTTCTTAAGA
TTACTGTAAGGTAACCCTTT

Downstream 100 bases:

>100_bases
CCCGGCCCAGTGGTTGCGGCGCAACCAGTTCCGTGATTTTCGTCGGCCGGTGATTGGCCGGGTGTCCGGCGGGGTAGTGC
ATCAAGCGGTGCCTTCGGCT

Product: EfpA_2

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux protein A [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTALNDSERAVQNWTSARPDRPAPVRSTPPAETAPKPAAETAVKRTSKYYPAWLPSRRFIAAVIAIGGMQLLATMDSTVA
IVALPRIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEATMVIARLSQG
VGSAIASPTGLALVATTFPKGPARNFATAVFAAMTAVGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMMYLARTALRETN
RERMKLDATGAVLATLACTAAVFAFSMGPEKGWISLTTISSGVVALGAALAFVIVERTAENPVVPFDLFRDRNRLVTFIA
IFLAGGVMFTLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVSSQLVARFSPRVLTIAGGYLLVLAMLYGWWCMH
RGVPYFPNLVLPIVVGGIGIGMAVVPLTLSAIAGVGFDQIGPVSAVTLMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPV
KTMNDAQLQALDHGYTYGLLWVAGAAVIVGAAALFIGYTPEQVAHAQEVKEAMDAGEL

Sequences:

>Translated_538_residues
MTALNDSERAVQNWTSARPDRPAPVRSTPPAETAPKPAAETAVKRTSKYYPAWLPSRRFIAAVIAIGGMQLLATMDSTVA
IVALPRIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEATMVIARLSQG
VGSAIASPTGLALVATTFPKGPARNFATAVFAAMTAVGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMMYLARTALRETN
RERMKLDATGAVLATLACTAAVFAFSMGPEKGWISLTTISSGVVALGAALAFVIVERTAENPVVPFDLFRDRNRLVTFIA
IFLAGGVMFTLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVSSQLVARFSPRVLTIAGGYLLVLAMLYGWWCMH
RGVPYFPNLVLPIVVGGIGIGMAVVPLTLSAIAGVGFDQIGPVSAVTLMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPV
KTMNDAQLQALDHGYTYGLLWVAGAAVIVGAAALFIGYTPEQVAHAQEVKEAMDAGEL
>Mature_537_residues
TALNDSERAVQNWTSARPDRPAPVRSTPPAETAPKPAAETAVKRTSKYYPAWLPSRRFIAAVIAIGGMQLLATMDSTVAI
VALPRIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEATMVIARLSQGV
GSAIASPTGLALVATTFPKGPARNFATAVFAAMTAVGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMMYLARTALRETNR
ERMKLDATGAVLATLACTAAVFAFSMGPEKGWISLTTISSGVVALGAALAFVIVERTAENPVVPFDLFRDRNRLVTFIAI
FLAGGVMFTLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVSSQLVARFSPRVLTIAGGYLLVLAMLYGWWCMHR
GVPYFPNLVLPIVVGGIGIGMAVVPLTLSAIAGVGFDQIGPVSAVTLMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVK
TMNDAQLQALDHGYTYGLLWVAGAAVIVGAAALFIGYTPEQVAHAQEVKEAMDAGEL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=461, Percent_Identity=26.6811279826464, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1790195, Length=408, Percent_Identity=27.4509803921569, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789042, Length=449, Percent_Identity=26.2806236080178, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081983, Length=367, Percent_Identity=26.4305177111717, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788392, Length=334, Percent_Identity=26.0479041916168, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323520, Length=368, Percent_Identity=23.9130434782609, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324954, Length=305, Percent_Identity=24.5901639344262, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323935, Length=384, Percent_Identity=23.1770833333333, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=25.7861635220126, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=139, Percent_Identity=33.0935251798561, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56398; Mature: 56267

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALNDSERAVQNWTSARPDRPAPVRSTPPAETAPKPAAETAVKRTSKYYPAWLPSRRFI
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
AAVIAIGGMQLLATMDSTVAIVALPRIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLG
HHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
DTIGRKRTFIVGVALFTISSVLCAVAWDEATMVIARLSQGVGSAIASPTGLALVATTFPK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GPARNFATAVFAAMTAVGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMMYLARTALRETN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
RERMKLDATGAVLATLACTAAVFAFSMGPEKGWISLTTISSGVVALGAALAFVIVERTAE
HHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHCCC
NPVVPFDLFRDRNRLVTFIAIFLAGGVMFTLTVCIGLYVQDILGYSALRAGVGFIPFVIA
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MGIGLGVSSQLVARFSPRVLTIAGGYLLVLAMLYGWWCMHRGVPYFPNLVLPIVVGGIGI
HHHCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GMAVVPLTLSAIAGVGFDQIGPVSAVTLMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCC
KTMNDAQLQALDHGYTYGLLWVAGAAVIVGAAALFIGYTPEQVAHAQEVKEAMDAGEL
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TALNDSERAVQNWTSARPDRPAPVRSTPPAETAPKPAAETAVKRTSKYYPAWLPSRRFI
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
AAVIAIGGMQLLATMDSTVAIVALPRIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLG
HHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
DTIGRKRTFIVGVALFTISSVLCAVAWDEATMVIARLSQGVGSAIASPTGLALVATTFPK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GPARNFATAVFAAMTAVGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMMYLARTALRETN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
RERMKLDATGAVLATLACTAAVFAFSMGPEKGWISLTTISSGVVALGAALAFVIVERTAE
HHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHCCC
NPVVPFDLFRDRNRLVTFIAIFLAGGVMFTLTVCIGLYVQDILGYSALRAGVGFIPFVIA
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MGIGLGVSSQLVARFSPRVLTIAGGYLLVLAMLYGWWCMHRGVPYFPNLVLPIVVGGIGI
HHHCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GMAVVPLTLSAIAGVGFDQIGPVSAVTLMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCC
KTMNDAQLQALDHGYTYGLLWVAGAAVIVGAAALFIGYTPEQVAHAQEVKEAMDAGEL
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]