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The map label for this gene is miaB [H]

Identifier: 41408944

GI number: 41408944

Start: 3175301

End: 3176854

Strand: Reverse

Name: miaB [H]

Synonym: MAP2846c

Alternate gene names: 41408944

Gene position: 3176854-3175301 (Counterclockwise)

Preceding gene: 161611202

Following gene: 41408943

Centisome position: 65.78

GC content: 72.07

Gene sequence:

>1554_bases
ATGACGTGCGGTTTCTCCCGTGCGGACCGCTCGCCGTACCATGGCCCCGTGACTTCGACGGTGGCCCGGGATGTTTCGGG
GGTGCGCACCTATCAGGTGCGCACCTACGGCTGCCAGATGAACGTGCACGACTCCGAGCGGCTGGCGGGTCTGCTGGAGG
CGGCCGGCTACCGGCGGGCCGCCGAGGGGGCTGAGGTCGCCGACGTGGTGGTGTTCAACACCTGCGCGGTCCGGGAGAAC
GCCGACAACAAGCTGTACGGCAACCTCAGCCACCTGGCGCCGCGCAAGCGGGGCAACCCGGAGATGCAGATCGCGGTCGG
TGGCTGCCTGGCCCAGAAGGACCGCGAGGCCGTGCTGCGCCGGGCGCCCTGGGTCGACGTCGTGTTCGGCACCCACAACA
TCGGGTCGCTGCCCACCCTGCTGGAGCGGGCCCGGCACAACAAGGCCGCCCAGGTGGAGATCGCCGAGGCGCTGCAGCAG
TTCCCCTCGTCGCTGCCCAGTGCGCGCGAATCCGCTTATGCCGCTTGGGTTTCCATCTCGGTCGGCTGCAACAACAGCTG
CACGTTCTGCATCGTCCCGTCGCTGCGCGGCAAGGAGGTCGACCGCAGTCCCGACGACATCCTGGCCGAGGTCAGGTCCC
TGGTGGCCGACGGCGTCCTCGAGGTCACGCTGCTGGGTCAGAACGTCAACGCCTACGGGGTGTCCTTCGCCGACCCGGCG
CTGCCCCGCGACCGCGGCGCGTTCGCCCGACTGTTGCGGGCCTGCGGCGAGATCGACGGGCTGGAACGGGTGCGGTTCAC
CTCGCCGCATCCGGCCGAGTTCACCGACGACGTCATCGAGGCCATGGCGCAGACCCCGAACGTGTGCCCCGCCCTGCACA
TGCCGCTGCAGTCCGGCTCCGACCGGGTGCTGCGCGCGATGCGGCGCTCGTACCGCGCCGAGCGTTTCCTCGGCATCATC
GACCGGGTCCGGGCCGCCATGCCGCACGCCGCCATCACCACCGACCTGATCGTCGGGTTCCCCGGCGAGACCGAAGAGGA
CTTCGCGGCCACCCTGGACGTGGTGCGCCGGGCCCGGTTCGCGGCCGCCTTCACCTTCCAGTACTCCAAGCGGCCCGGCA
CCCCGGCCGCCGAGCTCGACGGGCAGATCCCGAAAGCCGTTGTGCAGGAACGCTATGAGCGCCTCGTCGAGCTGCAGGAG
TCGATCTCGTTGCAGGGCAACCAGGCCCTGGTCGGCCAGACCGTCGAGCTGCTGGTCGCCACGGGGGAGGGGCGCAAGGA
CAGCGCCACCGCCCGGATGAGCGGACGCGCCCGCGACGGCCGGCTGGTGCACTTCGCCGCCGACGACCGGGTGCGCCCCG
GCGACCTGGTCACCACGGTCATCACCGGGGCCGCACCGCACCACCTGATCGCCGACGCCGGCGTCCTCAGCCATCGCCGC
ACCCGGGCCGGTGACGCGCACGCGGCCGGCCGGCGCCCGCGCGGCGTCGGTCTCGGCATGCCCGCCGTCGGGCCGCCGGC
CGGCCCGGCCCAACCCGTTGGATGTGCCTCATGA

Upstream 100 bases:

>100_bases
GGTGGGGATGCTGGCCCGGCGCGGCTACAGCCAGAACCTGGCGTGCGAGGTGGTGCTGGCCGAGCTGGCCGCCGAACGGG
AGCGCCGCCGCGTCTAGCCG

Downstream 100 bases:

>100_bases
ACGACGACCGCAACCACGACGACTCCCAGCTGGGCGCGCTGCGCACCGAGATCGAGGCCGCCGAACGGCGGGTGGCCGGC
GGCATCGACCCCGGCGCCCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRAAEGAEVADVVVFNTCAVREN
ADNKLYGNLSHLAPRKRGNPEMQIAVGGCLAQKDREAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQ
FPSSLPSARESAYAAWVSISVGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA
LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRAMRRSYRAERFLGII
DRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQE
SISLQGNQALVGQTVELLVATGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGVLSHRR
TRAGDAHAAGRRPRGVGLGMPAVGPPAGPAQPVGCAS

Sequences:

>Translated_517_residues
MTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRAAEGAEVADVVVFNTCAVREN
ADNKLYGNLSHLAPRKRGNPEMQIAVGGCLAQKDREAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQ
FPSSLPSARESAYAAWVSISVGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA
LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRAMRRSYRAERFLGII
DRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQE
SISLQGNQALVGQTVELLVATGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGVLSHRR
TRAGDAHAAGRRPRGVGLGMPAVGPPAGPAQPVGCAS
>Mature_516_residues
TCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRAAEGAEVADVVVFNTCAVRENA
DNKLYGNLSHLAPRKRGNPEMQIAVGGCLAQKDREAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQF
PSSLPSARESAYAAWVSISVGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPAL
PRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRAMRRSYRAERFLGIID
RVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQES
ISLQGNQALVGQTVELLVATGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGVLSHRRT
RAGDAHAAGRRPRGVGLGMPAVGPPAGPAQPVGCAS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 64 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=35.4297693920335, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=35.4297693920335, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=28.6063569682152, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=40.8071748878924, Blast_Score=341, Evalue=8e-95,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=30.6666666666667, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=33.0490405117271, Blast_Score=228, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=224, Percent_Identity=36.6071428571429, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=36.0277136258661, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI19922432, Length=412, Percent_Identity=31.5533980582524, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55594; Mature: 55462

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRA
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCHHH
AEGAEVADVVVFNTCAVRENADNKLYGNLSHLAPRKRGNPEMQIAVGGCLAQKDREAVLR
CCCCCCEEEEEEEHEECCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
RAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQFPSSLPSARESAYAAWVSIS
HCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHEEEEEEEE
VGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA
ECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGS
CCCCCHHHHHHHHHHCCCCCCHHEEECCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCC
DRVLRAMRRSYRAERFLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHH
AAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESISLQGNQALVGQTVELLVA
HHEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEE
TGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGVLSHRR
ECCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
TRAGDAHAAGRRPRGVGLGMPAVGPPAGPAQPVGCAS
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRA
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCHHH
AEGAEVADVVVFNTCAVRENADNKLYGNLSHLAPRKRGNPEMQIAVGGCLAQKDREAVLR
CCCCCCEEEEEEEHEECCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
RAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQFPSSLPSARESAYAAWVSIS
HCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHEEEEEEEE
VGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA
ECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGS
CCCCCHHHHHHHHHHCCCCCCHHEEECCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCC
DRVLRAMRRSYRAERFLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHH
AAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESISLQGNQALVGQTVELLVA
HHEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEE
TGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGVLSHRR
ECCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
TRAGDAHAAGRRPRGVGLGMPAVGPPAGPAQPVGCAS
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA