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The map label for this gene is miaA

Identifier: 41408939

GI number: 41408939

Start: 3170309

End: 3171253

Strand: Reverse

Name: miaA

Synonym: MAP2841c

Alternate gene names: 41408939

Gene position: 3171253-3170309 (Counterclockwise)

Preceding gene: 41408940

Following gene: 41408938

Centisome position: 65.66

GC content: 75.77

Gene sequence:

>945_bases
GTGACGGCGGCCGTGCGGCCACTGCCGATCATCGGCCCCACCGGCACCGGCAAGTCCCAGCTGGCGCTGGACGTCGCCGA
ACGGCTCGGGCCACTCGGCGCCGAGATCGTCAACGCCGACGCGATGCAGCTCTACCGCGGGATGGACATCGGCACCGCCA
AGCTGCCCGTCGACGCCCGCCGCGGCATCCCGCACCACCAGCTCGACGTCCTCGACGTCACCCAGACCGCGACCGTCGCC
CGCTATCAGCGCGCCGCCGCGGCCGACATCGAGGCCATCCTGGCCGCCGGCGCGGTGCCGATCATCGTGGGCGGCTCCAT
GCTCTACATCCAGTCGCTGCTCGACGACTGGTCGTTCCCGGCCACCGATCCGCGGGTGCGGGCGCGCTGGGAGCGGCGGC
TGGGCGAGGTCGGGGTGGGCGAGCTGCACGCCGAGCTGGCCCGCCGCGATCCCGCGGCCGCCGCGGCGATCCTGCCCACC
GACGCCCGGCGCACGGTGCGGGCGCTGGAGGTCATCGAGCTGACCGGCCGGCCGTTCGCCGCGTCCGCGCCGCGGATCGG
CGCGCCGCGGTGGGACACCGTCATCATCGGATTGGATTGCGACACAACGCTTCTCGACGAGCGGCTGGCTAGGCGCACCG
ACGCGATGTTCGAGCAGGGCCTGGTGGCGGAGGTGACCGGGCTGCTCGGGCGGGGGCTGCGCGACGGGGTCACCGCCGCG
CGCGCCCTGGGCTACGCGCAGGTGATCGCCGCGCTCGACGCCGGCGGCGGCGACGAGCAGCTGCGGCAGGCCCGCGAGCA
GACCTACGCGGGCACCCGCCGCTACGTGCGCCGGCAGCGGTCCTGGTTTCGGCGCGACCACCGGGTGCGCTGGCTCGACG
CCGGCGCCTGCAGCCCGCCCCGCCTCGCCGACGCCGCCCTGGAGGCCTGGCGCCACGTATCCTGA

Upstream 100 bases:

>100_bases
GGGTGCTGGCCGGGCTGGCCGAGCCGGGGCCGCGCTCGGCCAAGGAGCTCTACCGGGGGGCGCCCTACGGGGTGGGCTAC
TTCGCCGGCGCCTGGCAGCT

Downstream 100 bases:

>100_bases
GCAGGTGAAATTCGCGAAGGGCCACGGCACCGAGAACGACTTCGTGCTGCTGTGCGACCCCCCGGCCGAGCTGCGCCTGA
CCGGGGCGGGCGTGGCCGCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTAAVRPLPIIGPTGTGKSQLALDVAERLGPLGAEIVNADAMQLYRGMDIGTAKLPVDARRGIPHHQLDVLDVTQTATVA
RYQRAAAADIEAILAAGAVPIIVGGSMLYIQSLLDDWSFPATDPRVRARWERRLGEVGVGELHAELARRDPAAAAAILPT
DARRTVRALEVIELTGRPFAASAPRIGAPRWDTVIIGLDCDTTLLDERLARRTDAMFEQGLVAEVTGLLGRGLRDGVTAA
RALGYAQVIAALDAGGGDEQLRQAREQTYAGTRRYVRRQRSWFRRDHRVRWLDAGACSPPRLADAALEAWRHVS

Sequences:

>Translated_314_residues
MTAAVRPLPIIGPTGTGKSQLALDVAERLGPLGAEIVNADAMQLYRGMDIGTAKLPVDARRGIPHHQLDVLDVTQTATVA
RYQRAAAADIEAILAAGAVPIIVGGSMLYIQSLLDDWSFPATDPRVRARWERRLGEVGVGELHAELARRDPAAAAAILPT
DARRTVRALEVIELTGRPFAASAPRIGAPRWDTVIIGLDCDTTLLDERLARRTDAMFEQGLVAEVTGLLGRGLRDGVTAA
RALGYAQVIAALDAGGGDEQLRQAREQTYAGTRRYVRRQRSWFRRDHRVRWLDAGACSPPRLADAALEAWRHVS
>Mature_313_residues
TAAVRPLPIIGPTGTGKSQLALDVAERLGPLGAEIVNADAMQLYRGMDIGTAKLPVDARRGIPHHQLDVLDVTQTATVAR
YQRAAAADIEAILAAGAVPIIVGGSMLYIQSLLDDWSFPATDPRVRARWERRLGEVGVGELHAELARRDPAAAAAILPTD
ARRTVRALEVIELTGRPFAASAPRIGAPRWDTVIIGLDCDTTLLDERLARRTDAMFEQGLVAEVTGLLGRGLRDGVTAAR
ALGYAQVIAALDAGGGDEQLRQAREQTYAGTRRYVRRQRSWFRRDHRVRWLDAGACSPPRLADAALEAWRHVS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=336, Percent_Identity=31.8452380952381, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=35.2941176470588, Blast_Score=157, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=299, Percent_Identity=26.0869565217391, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=27.7027027027027, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24649830, Length=340, Percent_Identity=31.1764705882353, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MYCPA (Q73W20)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961775.1
- ProteinModelPortal:   Q73W20
- SMR:   Q73W20
- EnsemblBacteria:   EBMYCT00000036703
- GeneID:   2717510
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2841c
- NMPDR:   fig|262316.1.peg.2841
- GeneTree:   EBGT00050000016412
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34025; Mature: 33894

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAVRPLPIIGPTGTGKSQLALDVAERLGPLGAEIVNADAMQLYRGMDIGTAKLPVDAR
CCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
RGIPHHQLDVLDVTQTATVARYQRAAAADIEAILAAGAVPIIVGGSMLYIQSLLDDWSFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCC
ATDPRVRARWERRLGEVGVGELHAELARRDPAAAAAILPTDARRTVRALEVIELTGRPFA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHCCCCCC
ASAPRIGAPRWDTVIIGLDCDTTLLDERLARRTDAMFEQGLVAEVTGLLGRGLRDGVTAA
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
RALGYAQVIAALDAGGGDEQLRQAREQTYAGTRRYVRRQRSWFRRDHRVRWLDAGACSPP
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
RLADAALEAWRHVS
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TAAVRPLPIIGPTGTGKSQLALDVAERLGPLGAEIVNADAMQLYRGMDIGTAKLPVDAR
CCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
RGIPHHQLDVLDVTQTATVARYQRAAAADIEAILAAGAVPIIVGGSMLYIQSLLDDWSFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCC
ATDPRVRARWERRLGEVGVGELHAELARRDPAAAAAILPTDARRTVRALEVIELTGRPFA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHCCCCCC
ASAPRIGAPRWDTVIIGLDCDTTLLDERLARRTDAMFEQGLVAEVTGLLGRGLRDGVTAA
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
RALGYAQVIAALDAGGGDEQLRQAREQTYAGTRRYVRRQRSWFRRDHRVRWLDAGACSPP
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
RLADAALEAWRHVS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA