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The map label for this gene is amiB2

Identifier: 41408607

GI number: 41408607

Start: 2817240

End: 2818625

Strand: Reverse

Name: amiB2

Synonym: MAP2509c

Alternate gene names: 41408607

Gene position: 2818625-2817240 (Counterclockwise)

Preceding gene: 41408611

Following gene: 41408605

Centisome position: 58.36

GC content: 73.09

Gene sequence:

>1386_bases
GTGAACGATCTCGCCTTCGCCGGCGCGGCCGCACAAGCGCGCATGCTGGCCAACGGTGACCTGAGCGCGCCGGAGCTGCT
CGAGGTGTACCTGGAGCGGATCGCGCGGCTGGACAGCCAGCTGCGCTGCTACCGCGTGGTGCTCGCCGACCGGGCCCGGC
GCGAGGCCGCGGCGGCGCAGTCCCGCCTGGACGCGGGCGAGCGGCTGCCGCTGCTCGGCGTGCCGGTCGCGATCAAGGAC
GACGTCGACGTCGCCGGGGAGGTGACCACCTTCGGCAGCGGCGGGCACGGACCCGCGGTCAGCTCCGACGCCGAGGTGGT
GCGCCGGCTGCGCGCGGCCGGCGCCGTCATCATCGGCAAAACCAATGTGCCCGAGCTGATGATGATGCCCTACACCGAGT
CGCTGACGTTCGGCGCGACGCGTAACCCTTGGGATCCGCGACGCACACCGGGTGGCAGCAGCGGCGGCAGCGCCGCGGCG
GTGGCGGCCGGGCTGGCGCCGCTGGCGTTGGGATCCGACGGCGGCGGCTCGATCCGCATCCCGTCGACCTGGTGCGGCCT
GTTCGGGCTGAAACCGCAACGGGATCGGGTGTCGCTGGAACCCCACGACGATGCCTGGTACGGCCTGAGCGTCAACGGCC
CGATCGCGCGGACGGTGATCGACGCGGCCGTGTTCCTCGACGCGACCTCCACGGTGCCCGGTCCGGAGGGGGAGTTCGTC
GCGGCGGCGACCCGCGAGCCCGGCCGGCTGCGAATCGCGTTGAGCACCAAGGTTCCCACCCCGCTGCCGGTGCGGGTCGG
CAAGCCGGAGCTGGCGGCGGTGCAGCAGGCGGGTGCGTTGCTGCGCGATCTCGGCCACGACGTCGTCACGGCCGACCCCG
AGTACCCGGCCGCGGCGATCCTGGGCAACTATCTGCCCCGCTATCTGCGCGGGATCTGCGACGACGCCGACTCGCTGGCC
CATCCGGAGCGGCTGGAGACACGCACCCGCAACATGGCCCGGATGGGCTCGTTCTTCTCGGACCGCCGGATGGCGGCCAT
CCGGGCCGGCGAGGGCCCGCTGATCGCGCGGATTCAGGCGATCTTCGACGACGTCGACGTCGTGGTCACCCCCGGCACCG
CGGCCGGCCCGTCCCGGGTGGGCGCCTATCAACGCCGCGGCGCGGTGTCCACACTGCTGCGGGTCGGGCAGTGCGTTCCC
TACCAACAGATCTGGAATCTGACCGGCCAACCGGCCGCGGTGGTGCCGTGGGACTTCGACGCCGACGGCCTGCCGATGTC
GGTCCAGCTGGTCGGGCGGCCCTACGACGAGGCGACCCTGTTGTCGCTGTCGGCGCAGATCGAGGCCGCGCGGCCGTGGG
CCCTGCGCCGCCCACCGGTCTGTTAA

Upstream 100 bases:

>100_bases
ACAGAACACGCAGGACATGTCGCCCACGGTACTGGTTGGCCCGCGACCGCCTCGTCCCCGCCGGCGCCGGCCCGGCCGAC
CCGCGGGATACGCTGCGGCG

Downstream 100 bases:

>100_bases
GCGGAATTCCGCAGCACCGCCCAGGCGGCTAGTTTGGCGCCCGGCGCCATCACGTGCGCCGGACTGGTCGACGGCAGCCG
CTGAAAGCAAACGTGATCGG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNDLAFAGAAAQARMLANGDLSAPELLEVYLERIARLDSQLRCYRVVLADRARREAAAAQSRLDAGERLPLLGVPVAIKD
DVDVAGEVTTFGSGGHGPAVSSDAEVVRRLRAAGAVIIGKTNVPELMMMPYTESLTFGATRNPWDPRRTPGGSSGGSAAA
VAAGLAPLALGSDGGGSIRIPSTWCGLFGLKPQRDRVSLEPHDDAWYGLSVNGPIARTVIDAAVFLDATSTVPGPEGEFV
AAATREPGRLRIALSTKVPTPLPVRVGKPELAAVQQAGALLRDLGHDVVTADPEYPAAAILGNYLPRYLRGICDDADSLA
HPERLETRTRNMARMGSFFSDRRMAAIRAGEGPLIARIQAIFDDVDVVVTPGTAAGPSRVGAYQRRGAVSTLLRVGQCVP
YQQIWNLTGQPAAVVPWDFDADGLPMSVQLVGRPYDEATLLSLSAQIEAARPWALRRPPVC

Sequences:

>Translated_461_residues
MNDLAFAGAAAQARMLANGDLSAPELLEVYLERIARLDSQLRCYRVVLADRARREAAAAQSRLDAGERLPLLGVPVAIKD
DVDVAGEVTTFGSGGHGPAVSSDAEVVRRLRAAGAVIIGKTNVPELMMMPYTESLTFGATRNPWDPRRTPGGSSGGSAAA
VAAGLAPLALGSDGGGSIRIPSTWCGLFGLKPQRDRVSLEPHDDAWYGLSVNGPIARTVIDAAVFLDATSTVPGPEGEFV
AAATREPGRLRIALSTKVPTPLPVRVGKPELAAVQQAGALLRDLGHDVVTADPEYPAAAILGNYLPRYLRGICDDADSLA
HPERLETRTRNMARMGSFFSDRRMAAIRAGEGPLIARIQAIFDDVDVVVTPGTAAGPSRVGAYQRRGAVSTLLRVGQCVP
YQQIWNLTGQPAAVVPWDFDADGLPMSVQLVGRPYDEATLLSLSAQIEAARPWALRRPPVC
>Mature_461_residues
MNDLAFAGAAAQARMLANGDLSAPELLEVYLERIARLDSQLRCYRVVLADRARREAAAAQSRLDAGERLPLLGVPVAIKD
DVDVAGEVTTFGSGGHGPAVSSDAEVVRRLRAAGAVIIGKTNVPELMMMPYTESLTFGATRNPWDPRRTPGGSSGGSAAA
VAAGLAPLALGSDGGGSIRIPSTWCGLFGLKPQRDRVSLEPHDDAWYGLSVNGPIARTVIDAAVFLDATSTVPGPEGEFV
AAATREPGRLRIALSTKVPTPLPVRVGKPELAAVQQAGALLRDLGHDVVTADPEYPAAAILGNYLPRYLRGICDDADSLA
HPERLETRTRNMARMGSFFSDRRMAAIRAGEGPLIARIQAIFDDVDVVVTPGTAAGPSRVGAYQRRGAVSTLLRVGQCVP
YQQIWNLTGQPAAVVPWDFDADGLPMSVQLVGRPYDEATLLSLSAQIEAARPWALRRPPVC

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=25.4032258064516, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI195972892, Length=189, Percent_Identity=30.6878306878307, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI166795287, Length=511, Percent_Identity=28.3757338551859, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=33.1932773109244, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=34.7593582887701, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=520, Percent_Identity=26.1538461538462, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=325, Percent_Identity=27.3846153846154, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=201, Percent_Identity=32.3383084577114, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=186, Percent_Identity=33.8709677419355, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=128, Percent_Identity=42.96875, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=274, Percent_Identity=29.5620437956204, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32565337, Length=496, Percent_Identity=21.3709677419355, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=241, Percent_Identity=34.4398340248963, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319685, Length=384, Percent_Identity=27.0833333333333, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=183, Percent_Identity=39.344262295082, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648435, Length=238, Percent_Identity=31.9327731092437, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648437, Length=238, Percent_Identity=31.9327731092437, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI45550774, Length=238, Percent_Identity=31.9327731092437, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=27.8455284552846, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=37.5, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=37.5, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652985, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922090, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652981, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652983, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=229, Percent_Identity=29.6943231441048, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648113, Length=462, Percent_Identity=30.0865800865801, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 48819; Mature: 48819

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLAFAGAAAQARMLANGDLSAPELLEVYLERIARLDSQLRCYRVVLADRARREAAAAQ
CCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLDAGERLPLLGVPVAIKDDVDVAGEVTTFGSGGHGPAVSSDAEVVRRLRAAGAVIIGK
HHCCCCCCCCEEECCEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEEC
TNVPELMMMPYTESLTFGATRNPWDPRRTPGGSSGGSAAAVAAGLAPLALGSDGGGSIRI
CCCCCEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCCCEEEC
PSTWCGLFGLKPQRDRVSLEPHDDAWYGLSVNGPIARTVIDAAVFLDATSTVPGPEGEFV
CHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHEECCCCCCCCCCCEE
AAATREPGRLRIALSTKVPTPLPVRVGKPELAAVQQAGALLRDLGHDVVTADPEYPAAAI
EEECCCCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHH
LGNYLPRYLRGICDDADSLAHPERLETRTRNMARMGSFFSDRRMAAIRAGEGPLIARIQA
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
IFDDVDVVVTPGTAAGPSRVGAYQRRGAVSTLLRVGQCVPYQQIWNLTGQPAAVVPWDFD
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCC
ADGLPMSVQLVGRPYDEATLLSLSAQIEAARPWALRRPPVC
CCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDLAFAGAAAQARMLANGDLSAPELLEVYLERIARLDSQLRCYRVVLADRARREAAAAQ
CCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLDAGERLPLLGVPVAIKDDVDVAGEVTTFGSGGHGPAVSSDAEVVRRLRAAGAVIIGK
HHCCCCCCCCEEECCEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEEC
TNVPELMMMPYTESLTFGATRNPWDPRRTPGGSSGGSAAAVAAGLAPLALGSDGGGSIRI
CCCCCEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCCCEEEC
PSTWCGLFGLKPQRDRVSLEPHDDAWYGLSVNGPIARTVIDAAVFLDATSTVPGPEGEFV
CHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHEECCCCCCCCCCCEE
AAATREPGRLRIALSTKVPTPLPVRVGKPELAAVQQAGALLRDLGHDVVTADPEYPAAAI
EEECCCCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHH
LGNYLPRYLRGICDDADSLAHPERLETRTRNMARMGSFFSDRRMAAIRAGEGPLIARIQA
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
IFDDVDVVVTPGTAAGPSRVGAYQRRGAVSTLLRVGQCVPYQQIWNLTGQPAAVVPWDFD
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCC
ADGLPMSVQLVGRPYDEATLLSLSAQIEAARPWALRRPPVC
CCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]