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The map label for this gene is rph

Identifier: 41408520

GI number: 41408520

Start: 2719336

End: 2720115

Strand: Reverse

Name: rph

Synonym: MAP2422c

Alternate gene names: 41408520

Gene position: 2720115-2719336 (Counterclockwise)

Preceding gene: 41408521

Following gene: 41408519

Centisome position: 56.32

GC content: 71.92

Gene sequence:

>780_bases
GTGACGCGACGAGAAGACGGCCGCCTGGACGACGAGCTTCGCCCGCTGGTGATCACCCGCGGGTTCACCGAACACCCCGC
CGGTTCGGTGCTGATCGAATTCGGCCACACCAAGGTGATGTGCACGGCCAGCGTCACCGAGGGCGTGCCGCGCTGGCGCA
AGGGCTCGGGCCTGGGCTGGCTGACCGCCGAGTACGCGATGTTGCCGTCGGCGACGCACACCCGTTCGGACCGCGAATCG
GTCAAGGGCCGGCTGTCCGGGCGCACCCAGGAGATCAGCCGGCTCATCGGTCGGTCGCTGCGGGCCTGCATCGACCTGGC
GGCGCTGGGGGAGAACACGATCGCCGTCGACTGCGACGTGCTGCAGGCCGACGGCGGCACCCGCACCGCGGCAATCACCG
GCGCCTTCGTGGCGCTGGCCGACGCCGTCACCTACCTGTCGGCGGCGGGGAAACTGTCCGATCCGCGGCCGTTGTCGTGT
GCCATCGCGGCGGTCAGCGTCGGCGTGGTCGACGGCCGGATCCGGGTGGACCTGCCCTACGAGGAGGACGCCCGTGCCGA
GGTCGACATGAACGTCGTCGCCACCGACACCGGCACGCTGGTGGAGGTGCAGGGCACCGGCGAGGGGGCGACCTTCCCGC
GTTCGACCCTGGACAAACTGCTCGACGCGGCGCTGGCCGCCTGCGACAAGCTGTTCGCCGCCCAGCGCGAGGCGCTCAAG
CTGCCCTACCCGGGGGTGTTGCCCGAGGGGCCGCCGCCGCCGAAGGCGTTCGGCAGCTGA

Upstream 100 bases:

>100_bases
CGACGGCCCCGTGCACGCGGTGGTGTGCGGCGAGACGTTCGACGTCCGCCGTTCCGAGAGGGTCTGAGCCGACCGGTTCG
AGGTGGCTTAGGGTTACTGG

Downstream 100 bases:

>100_bases
CCCGCCTGCCGGTGACCAGTGTTTCCGTGTGATACCCGCCCGACATGCGCGTAGCGTTGTGGGCTTGGAGGTGCCGCATG
AGCGACGACGCCGAACGGCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTRREDGRLDDELRPLVITRGFTEHPAGSVLIEFGHTKVMCTASVTEGVPRWRKGSGLGWLTAEYAMLPSATHTRSDRES
VKGRLSGRTQEISRLIGRSLRACIDLAALGENTIAVDCDVLQADGGTRTAAITGAFVALADAVTYLSAAGKLSDPRPLSC
AIAAVSVGVVDGRIRVDLPYEEDARAEVDMNVVATDTGTLVEVQGTGEGATFPRSTLDKLLDAALAACDKLFAAQREALK
LPYPGVLPEGPPPPKAFGS

Sequences:

>Translated_259_residues
MTRREDGRLDDELRPLVITRGFTEHPAGSVLIEFGHTKVMCTASVTEGVPRWRKGSGLGWLTAEYAMLPSATHTRSDRES
VKGRLSGRTQEISRLIGRSLRACIDLAALGENTIAVDCDVLQADGGTRTAAITGAFVALADAVTYLSAAGKLSDPRPLSC
AIAAVSVGVVDGRIRVDLPYEEDARAEVDMNVVATDTGTLVEVQGTGEGATFPRSTLDKLLDAALAACDKLFAAQREALK
LPYPGVLPEGPPPPKAFGS
>Mature_258_residues
TRREDGRLDDELRPLVITRGFTEHPAGSVLIEFGHTKVMCTASVTEGVPRWRKGSGLGWLTAEYAMLPSATHTRSDRESV
KGRLSGRTQEISRLIGRSLRACIDLAALGENTIAVDCDVLQADGGTRTAAITGAFVALADAVTYLSAAGKLSDPRPLSCA
IAAVSVGVVDGRIRVDLPYEEDARAEVDMNVVATDTGTLVEVQGTGEGATFPRSTLDKLLDAALAACDKLFAAQREALKL
PYPGVLPEGPPPPKAFGS

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=60.2870813397129, Blast_Score=254, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI71981632, Length=241, Percent_Identity=26.9709543568465, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27375; Mature: 27244

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRREDGRLDDELRPLVITRGFTEHPAGSVLIEFGHTKVMCTASVTEGVPRWRKGSGLGW
CCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCEEEEEEEHHHHCCCCCCCCCCCEE
LTAEYAMLPSATHTRSDRESVKGRLSGRTQEISRLIGRSLRACIDLAALGENTIAVDCDV
EEHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
LQADGGTRTAAITGAFVALADAVTYLSAAGKLSDPRPLSCAIAAVSVGVVDGRIRVDLPY
EECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEEEEECCEEEEECCC
EEDARAEVDMNVVATDTGTLVEVQGTGEGATFPRSTLDKLLDAALAACDKLFAAQREALK
CCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPYPGVLPEGPPPPKAFGS
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRREDGRLDDELRPLVITRGFTEHPAGSVLIEFGHTKVMCTASVTEGVPRWRKGSGLGW
CCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCEEEEEEEHHHHCCCCCCCCCCCEE
LTAEYAMLPSATHTRSDRESVKGRLSGRTQEISRLIGRSLRACIDLAALGENTIAVDCDV
EEHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
LQADGGTRTAAITGAFVALADAVTYLSAAGKLSDPRPLSCAIAAVSVGVVDGRIRVDLPY
EECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEEEEECCEEEEECCC
EEDARAEVDMNVVATDTGTLVEVQGTGEGATFPRSTLDKLLDAALAACDKLFAAQREALK
CCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPYPGVLPEGPPPPKAFGS
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA