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The map label for this gene is dapE [H]

Identifier: 41408428

GI number: 41408428

Start: 2612850

End: 2614271

Strand: Reverse

Name: dapE [H]

Synonym: MAP2330c

Alternate gene names: 41408428

Gene position: 2614271-2612850 (Counterclockwise)

Preceding gene: 41408429

Following gene: 41408426

Centisome position: 54.13

GC content: 70.6

Gene sequence:

>1422_bases
TTGAGCGCGAGCAGACACAATAGCGCCGCGACACGCCGTGAAGAAGGGGCTTTTGCGTCTGCTCGCGAGATAACGGAGCG
GGTGCGCGAGGTGCTGCCGTCGGTGCGGCTCGACCTGGAGGAGCTGGTGCGCATCGAATCGGTGTGGGCCGACCCCGGGC
GACGGCCCGAGGTGCAGCGCAGCGCACGGGCGGTGGCAGATCTGTTGGCGCAGGCCGGTTTCGGCGATGTGCGGATCGTC
AGCGAAGGCGGCGCGCCGGCGGTCATCGCCCGCCATCCCGCGCCGCCCGGCGCGCCCACCGTGCTGTTATATGCCCACCA
CGACGTGCAGCCCGAAGGCGACCGCGGGCAATGGGTCTCGCCGCCCTTCGAGCCCACCGAACGCGACGGCCGGCTCTATG
GCCGCGGCAGCGCCGACGACAAGGCCGGCATCGCAACACATTTGGCGGCGTTCCGGGCGCACGGCGGCCGGCCGCCGGTG
GGGGTGACGGTCTTCGTAGAGGGCGAAGAGGAATCCGGCTCGCCGTCGTTGGGTCGGTTGCTGGCCGCGCACCGCGACAC
CCTGGCCGCCGACGTGATTGTCATTGCCGACTCGGACAACTGGAGCGTCGACACGCCGGCGTTGACGGTGTCGCTGCGCG
GCCTGGTCGACTGCGTGGTCGAGGTTGCCACCCTCGACCACGGTCTGCACTCCGGGTTGTGGGGTGGGGTGGTGCCCGAC
GCGCTGAGCGTGCTGGTGCGCCTGCTGGCCAGCCTGCACGACGACGACGGCAACGTGGCCGTCGCCGGACTGCATGAGAC
AGACATCGCCACGCTGAACTATCCCGACTATCCGCCCGAGCGGGTCCGCGCCGATTCGGGCTTGCTGCCAGGCGTGTCCG
AGATCGGCTCGGGCTCAGTGCCGCAACGGCTTTGGGCCAAACCGGCGATCACCGTGATCGGCATCGACGCCACACCCATC
GAGCGGGCGTCCAACACGCTGATCCCGCGGGCCCGTGCCAAGATCAGCATGCGGGTGGCGCCCGGCGGCGACGCGGCGGC
GCACCTGGACGCGCTGACCGCGCACCTGCAACAGCACGCGCCGTGGGGCGCACAGGTCAGCGTCACCCGCGGGGACATCG
GCGAACCCTACGCGATCGACGCCAGCGGCAGCGTCTACGACGCCGCCCGCGCGGCCTTCCGCCAGGCGTGGGGCGCCGAG
CCGATCGACATGGGCATGGGCGGCTCCATCCCGTTCATCGCCGAATTCGCGGCCGCCTTCCCGGAGGCGAAGATTCTGGT
CACCGGCGTGGAAGACCCGGCGACCCAAGCGCACAGCATCAACGAGAGCCTGCATCTGGGCGTGCTGGAACGCGCGGCGA
TTAGCGAGGCGCTGCTGCTGACCAACCTGGCCAACCTGGCTGACCTGCTCAACCCGGGCTGA

Upstream 100 bases:

>100_bases
TGAGCGGTGACATCGCCAAGCACCTGGCCGACGTGACGATTCACGTGTCGCTGACGCACGAGAAAGACACCGCCGCCGCG
GTGGCGATCCTGGAGACTCC

Downstream 100 bases:

>100_bases
CTAGACCGACAGGTCGCGGCGGAGCTTGGCGACGTGACCGGTGGCCTTCACGTTGTACTGGGCGACGGCGATCCTGCCCT
TCTCGTCGACCACGAAGGTG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSASRHNSAATRREEGAFASAREITERVREVLPSVRLDLEELVRIESVWADPGRRPEVQRSARAVADLLAQAGFGDVRIV
SEGGAPAVIARHPAPPGAPTVLLYAHHDVQPEGDRGQWVSPPFEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGRPPV
GVTVFVEGEEESGSPSLGRLLAAHRDTLAADVIVIADSDNWSVDTPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPD
ALSVLVRLLASLHDDDGNVAVAGLHETDIATLNYPDYPPERVRADSGLLPGVSEIGSGSVPQRLWAKPAITVIGIDATPI
ERASNTLIPRARAKISMRVAPGGDAAAHLDALTAHLQQHAPWGAQVSVTRGDIGEPYAIDASGSVYDAARAAFRQAWGAE
PIDMGMGGSIPFIAEFAAAFPEAKILVTGVEDPATQAHSINESLHLGVLERAAISEALLLTNLANLADLLNPG

Sequences:

>Translated_473_residues
MSASRHNSAATRREEGAFASAREITERVREVLPSVRLDLEELVRIESVWADPGRRPEVQRSARAVADLLAQAGFGDVRIV
SEGGAPAVIARHPAPPGAPTVLLYAHHDVQPEGDRGQWVSPPFEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGRPPV
GVTVFVEGEEESGSPSLGRLLAAHRDTLAADVIVIADSDNWSVDTPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPD
ALSVLVRLLASLHDDDGNVAVAGLHETDIATLNYPDYPPERVRADSGLLPGVSEIGSGSVPQRLWAKPAITVIGIDATPI
ERASNTLIPRARAKISMRVAPGGDAAAHLDALTAHLQQHAPWGAQVSVTRGDIGEPYAIDASGSVYDAARAAFRQAWGAE
PIDMGMGGSIPFIAEFAAAFPEAKILVTGVEDPATQAHSINESLHLGVLERAAISEALLLTNLANLADLLNPG
>Mature_472_residues
SASRHNSAATRREEGAFASAREITERVREVLPSVRLDLEELVRIESVWADPGRRPEVQRSARAVADLLAQAGFGDVRIVS
EGGAPAVIARHPAPPGAPTVLLYAHHDVQPEGDRGQWVSPPFEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGRPPVG
VTVFVEGEEESGSPSLGRLLAAHRDTLAADVIVIADSDNWSVDTPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPDA
LSVLVRLLASLHDDDGNVAVAGLHETDIATLNYPDYPPERVRADSGLLPGVSEIGSGSVPQRLWAKPAITVIGIDATPIE
RASNTLIPRARAKISMRVAPGGDAAAHLDALTAHLQQHAPWGAQVSVTRGDIGEPYAIDASGSVYDAARAAFRQAWGAEP
IDMGMGGSIPFIAEFAAAFPEAKILVTGVEDPATQAHSINESLHLGVLERAAISEALLLTNLANLADLLNPG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=437, Percent_Identity=32.9519450800915, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI21071039, Length=474, Percent_Identity=29.535864978903, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI271398277, Length=303, Percent_Identity=31.3531353135314, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1788816, Length=130, Percent_Identity=32.3076923076923, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17563146, Length=438, Percent_Identity=30.1369863013699, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI193211059, Length=458, Percent_Identity=28.6026200873362, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321159, Length=464, Percent_Identity=27.801724137931, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319758, Length=493, Percent_Identity=24.3407707910751, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24585880, Length=435, Percent_Identity=31.9540229885057, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49720; Mature: 49589

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASRHNSAATRREEGAFASAREITERVREVLPSVRLDLEELVRIESVWADPGRRPEVQR
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHH
SARAVADLLAQAGFGDVRIVSEGGAPAVIARHPAPPGAPTVLLYAHHDVQPEGDRGQWVS
HHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
PPFEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRL
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHH
LAAHRDTLAADVIVIADSDNWSVDTPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPD
HHHHHCCCEEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ALSVLVRLLASLHDDDGNVAVAGLHETDIATLNYPDYPPERVRADSGLLPGVSEIGSGSV
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHHHCCCCCCCCCHHHHCCCCC
PQRLWAKPAITVIGIDATPIERASNTLIPRARAKISMRVAPGGDAAAHLDALTAHLQQHA
CHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
PWGAQVSVTRGDIGEPYAIDASGSVYDAARAAFRQAWGAEPIDMGMGGSIPFIAEFAAAF
CCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PEAKILVTGVEDPATQAHSINESLHLGVLERAAISEALLLTNLANLADLLNPG
CCCEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SASRHNSAATRREEGAFASAREITERVREVLPSVRLDLEELVRIESVWADPGRRPEVQR
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHH
SARAVADLLAQAGFGDVRIVSEGGAPAVIARHPAPPGAPTVLLYAHHDVQPEGDRGQWVS
HHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
PPFEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRL
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHH
LAAHRDTLAADVIVIADSDNWSVDTPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPD
HHHHHCCCEEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ALSVLVRLLASLHDDDGNVAVAGLHETDIATLNYPDYPPERVRADSGLLPGVSEIGSGSV
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHHHCCCCCCCCCHHHHCCCCC
PQRLWAKPAITVIGIDATPIERASNTLIPRARAKISMRVAPGGDAAAHLDALTAHLQQHA
CHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
PWGAQVSVTRGDIGEPYAIDASGSVYDAARAAFRQAWGAEPIDMGMGGSIPFIAEFAAAF
CCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PEAKILVTGVEDPATQAHSINESLHLGVLERAAISEALLLTNLANLADLLNPG
CCCEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]