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The map label for this gene is cysK

Identifier: 41408221

GI number: 41408221

Start: 2352623

End: 2353555

Strand: Direct

Name: cysK

Synonym: MAP2123

Alternate gene names: 41408221

Gene position: 2352623-2353555 (Clockwise)

Preceding gene: 41408220

Following gene: 41408222

Centisome position: 48.71

GC content: 68.49

Gene sequence:

>933_bases
ATGAGCATCGCCGAGAACGTCACGCAGCTGATCGGAAACACCCCGCTGGTCCGGTTGAACCGGGTCACCGAAGGTGCGGT
TGCCGACGTCGTCGCCAAGTTGGAATTCTTCAACCCGGGCAACAGCGTGAAGGACCGTATCGGGGTGGCCATGATCGATG
CGGCCGAGCAGGCGGGCCTGATCAAACCGGACACCATCATTCTCGAGCCGACGAGCGGCAACACCGGTATCGCGCTCGCA
CTGGTGGCCGCGGCGCGCGGCTACCGCTGCGTGCTCACCATGCCGGAGACGATGAGCGTGGAGCGGCGAATGTTGTTGCG
CGCCTTGGGCGCCGAGATCGTGCTGACGCCGGGCGCCGACGGCATGCCGGGCGCGATCGCCAAGGCCGAGGAATTGGCCA
AGAGCGACGACCGGTATTTCGTGCCGCAGCAGTTCGAGAACCCGGCCAACCCGGCTATTCACCGCAGCACCACCGCGGAG
GAAGTGTGGCGCGACACCGACGGCAAGGTCGACATCTTCGTCGCTGGCGTCGGCACCGGCGGCACCATCACCGGTGTCGC
CCAAGTCATCAAGGAACGCAAGCCGTCGGCGCAGTTCATCGCCGTGGAGCCGGCCGCGTCGCCGGTATTGTCCGGCGGCC
AGAAGGGGCCGCATCCGATCCAGGGTCTCGGCGCCGGGTTCGTGCCGCCGGTGCTTGCCATGGATCTGGTCGACGAGGTC
ATCGCCGTCGGCAACGAGGAGTCGATCGCGCTGGCCCGCCGGCTGGCCGCCGAGGAGGGGCTGCTGGTCGGCATCTCCTC
GGGCGCGGCGCTGGTGGCCGCCCTGCAGGTGGCCCGCCGGCCCGAGAACGCCGGCAAGCTGGTGGTCGTCGTGCTCCCCG
ACTTCGGTGAGCGGTACCTGAGCACGCCGCTGTTCGCCGACCTGGCCGATTAG

Upstream 100 bases:

>100_bases
CCCCGTTGCGCTAGCCATCCCCACCGAAATGGCCATAGAGTTCGATATTAGCTGGCAGGCCGACGAGGCCTGGTCCTACA
TCGTGGGAGACAAAAGGTAG

Downstream 100 bases:

>100_bases
CATGCTGGCAGCCATCCGTCGTGACATCAGGGCGGCCCGGGAGCGCGATCCGGCCGCTCCGACCACGCTGCAGGTGATCT
TCGCCTACCCGGGCGTGCAC

Product: hypothetical protein

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSIAENVTQLIGNTPLVRLNRVTEGAVADVVAKLEFFNPGNSVKDRIGVAMIDAAEQAGLIKPDTIILEPTSGNTGIALA
LVAAARGYRCVLTMPETMSVERRMLLRALGAEIVLTPGADGMPGAIAKAEELAKSDDRYFVPQQFENPANPAIHRSTTAE
EVWRDTDGKVDIFVAGVGTGGTITGVAQVIKERKPSAQFIAVEPAASPVLSGGQKGPHPIQGLGAGFVPPVLAMDLVDEV
IAVGNEESIALARRLAAEEGLLVGISSGAALVAALQVARRPENAGKLVVVVLPDFGERYLSTPLFADLAD

Sequences:

>Translated_310_residues
MSIAENVTQLIGNTPLVRLNRVTEGAVADVVAKLEFFNPGNSVKDRIGVAMIDAAEQAGLIKPDTIILEPTSGNTGIALA
LVAAARGYRCVLTMPETMSVERRMLLRALGAEIVLTPGADGMPGAIAKAEELAKSDDRYFVPQQFENPANPAIHRSTTAE
EVWRDTDGKVDIFVAGVGTGGTITGVAQVIKERKPSAQFIAVEPAASPVLSGGQKGPHPIQGLGAGFVPPVLAMDLVDEV
IAVGNEESIALARRLAAEEGLLVGISSGAALVAALQVARRPENAGKLVVVVLPDFGERYLSTPLFADLAD
>Mature_309_residues
SIAENVTQLIGNTPLVRLNRVTEGAVADVVAKLEFFNPGNSVKDRIGVAMIDAAEQAGLIKPDTIILEPTSGNTGIALAL
VAAARGYRCVLTMPETMSVERRMLLRALGAEIVLTPGADGMPGAIAKAEELAKSDDRYFVPQQFENPANPAIHRSTTAEE
VWRDTDGKVDIFVAGVGTGGTITGVAQVIKERKPSAQFIAVEPAASPVLSGGQKGPHPIQGLGAGFVPPVLAMDLVDEVI
AVGNEESIALARRLAAEEGLLVGISSGAALVAALQVARRPENAGKLVVVVLPDFGERYLSTPLFADLAD

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=42.5806451612903, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=42.5806451612903, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=42.5806451612903, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=57.7777777777778, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=41.4715719063545, Blast_Score=224, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=52.1452145214521, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=54.8701298701299, Blast_Score=313, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=50.1661129568106, Blast_Score=303, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI32566674, Length=291, Percent_Identity=51.2027491408935, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=49.1638795986622, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI32566672, Length=217, Percent_Identity=49.7695852534562, Blast_Score=220, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=42.1052631578947, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI25147552, Length=323, Percent_Identity=37.1517027863777, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=46.2809917355372, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321594, Length=316, Percent_Identity=37.9746835443038, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=36.085626911315, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643623, Length=310, Percent_Identity=42.258064516129, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI20129101, Length=310, Percent_Identity=42.258064516129, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32347; Mature: 32215

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAENVTQLIGNTPLVRLNRVTEGAVADVVAKLEFFNPGNSVKDRIGVAMIDAAEQAGL
CCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCEEEEECHHHCCC
IKPDTIILEPTSGNTGIALALVAAARGYRCVLTMPETMSVERRMLLRALGAEIVLTPGAD
CCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCC
GMPGAIAKAEELAKSDDRYFVPQQFENPANPAIHRSTTAEEVWRDTDGKVDIFVAGVGTG
CCCCHHHHHHHHHCCCCCEECCHHCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCC
GTITGVAQVIKERKPSAQFIAVEPAASPVLSGGQKGPHPIQGLGAGFVPPVLAMDLVDEV
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHH
IAVGNEESIALARRLAAEEGLLVGISSGAALVAALQVARRPENAGKLVVVVLPDFGERYL
HHCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
STPLFADLAD
CCCCHHCCCC
>Mature Secondary Structure 
SIAENVTQLIGNTPLVRLNRVTEGAVADVVAKLEFFNPGNSVKDRIGVAMIDAAEQAGL
CHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCEEEEECHHHCCC
IKPDTIILEPTSGNTGIALALVAAARGYRCVLTMPETMSVERRMLLRALGAEIVLTPGAD
CCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCC
GMPGAIAKAEELAKSDDRYFVPQQFENPANPAIHRSTTAEEVWRDTDGKVDIFVAGVGTG
CCCCHHHHHHHHHCCCCCEECCHHCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCC
GTITGVAQVIKERKPSAQFIAVEPAASPVLSGGQKGPHPIQGLGAGFVPPVLAMDLVDEV
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHH
IAVGNEESIALARRLAAEEGLLVGISSGAALVAALQVARRPENAGKLVVVVLPDFGERYL
HHCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
STPLFADLAD
CCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]