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The map label for this gene is glnA1

Identifier: 41408060

GI number: 41408060

Start: 2165225

End: 2166661

Strand: Direct

Name: glnA1

Synonym: MAP1962

Alternate gene names: 41408060

Gene position: 2165225-2166661 (Clockwise)

Preceding gene: 41408058

Following gene: 41408068

Centisome position: 44.83

GC content: 64.3

Gene sequence:

>1437_bases
GTGACGGAAACGACGCCCGACGACGTCTTCAAACTCGCCAAGGACGAGAAAGTCGAATTTGTCGACGTCCGGTTCTGTGA
CCTGCCCGGCATCATGCAGCACTTCACAATCCCGGTTTCGTTTTTCGACGAGAGCGTCTTCGAGGACGGCCTGGCCTTCG
ACGGCTCGTCCATTCGCGGATTCCAGTCCATTCACGAATCCGACATGTTGCTCCTGCCGGACCCGGCCACGGCCCAGATC
GACCTGTTCCGCGAGGCCAAGACGCTGAACCTGAACTTCTTCGTGCACGACCCGTTCACCCTCGAGCCGTACTCGCGCGA
CCCGCGCAACATCGCCCGCAAGGCGGAGAACTACCTGATCTCCACCGGTGTGGCCGACACCGCGTACTTCGGCGCCGAGG
CCGAGTTCTACATCTTCGACTCGGTGAGCTTCGACTCGCGCACCAACGGCTCGTTCTACGAGATCGACGCCATCTCGGGC
TGGTGGAACACCGGCTCGCCCAACGAACTCGACGGCAGCCCCAACCGCGGCTACAAGGTCCGCCCCAAGGGGGGCTACTT
CCCCGTCGCCCCCGTCGACCACTACGTCGACCTGCGCGACCAGATGCTGTCCAACCTGATCAAGGCCGGCTTCAGCCTGG
AGAAGGGCCACCACGAGGTGGGCACTGGCGGCCAGGCCGAGATCAACTACAAGTTCAACACGCTGCTGCACGCGGCCGAC
GACATGATGCTCTACAAGTACATCGTCAAGAACACCGCGTGGCAGAACGGCAAGACCGTCACGTTCATGCCCAAGCCGCT
GTTCGGGGACAACGGCTCCGGCATGCACACCCACCAGTCGCTGTGGAAGGACGGCAGCCCGCTGATGTACGACGAGACGG
GCTACGCCGGTCTGTCGGACACCGCGCGGCACTACATCGGCGGCCTGCTGCACCACGCGCCGTCGCTGCTGGCGTTCACC
AACCCGACGACGAACTCCTACAAGCGCCTGGTGCCGGGCTTCGAGGCGCCGATCAACCTGGTCTACAGCCAGCGCAACCG
CTCGGCGTGTGTGCGCATCCCGATCACCGGCACCAACCCGAAGGCCAAGCGGCTCGAGTTCCGTTGCCCCGACTCCTCGG
GCAACCCGTACCTGGCGTTCTCGGCCATGCTGATGGCCGGCCTGGACGGCATCAAGAACAAGATCGAGCCGCAGGCGCCG
GTCGACAAGGACCTCTACGAGCTGCCGCCCGAGGAGGCCGCCAACATCCCGCAGGCGCCGACCCAGCTGTCCGCGGTCAT
CGACCGGCTGGAAGAGGACCACGAATACCTCACCGAGGGAGGCGTTTTCACGCCCGACCTGATCGAGACGTGGATCAGCT
TCAAGCGCGAGACCGAGATCCTGCCGGTCCAGGTCCGGCCGCACCCCTACGAGTTCGCGCTGTACTACGACGTTTAG

Upstream 100 bases:

>100_bases
GGCATGGTCACGTAACGTGCGCGCAACATCGGGTTGACTGACGGGCAACATCTGCTCCATAGCGTCAGGCCGCGGATCTC
ACCAAGTAAAGGAGCATTCT

Downstream 100 bases:

>100_bases
CGACGTCTAAGTCGATGCCGCCCCTGCTGGGTTCCGCGAGACGTAAGCCATGGCGGCCCGCGCCGGCGTGTCGGCGCCAC
CGGGTAAGTGTCGTGGGGCT

Product: GlnA1

Products: NA

Alternate protein names: Glutamate--ammonia ligase 1 [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTETTPDDVFKLAKDEKVEFVDVRFCDLPGIMQHFTIPVSFFDESVFEDGLAFDGSSIRGFQSIHESDMLLLPDPATAQI
DLFREAKTLNLNFFVHDPFTLEPYSRDPRNIARKAENYLISTGVADTAYFGAEAEFYIFDSVSFDSRTNGSFYEIDAISG
WWNTGSPNELDGSPNRGYKVRPKGGYFPVAPVDHYVDLRDQMLSNLIKAGFSLEKGHHEVGTGGQAEINYKFNTLLHAAD
DMMLYKYIVKNTAWQNGKTVTFMPKPLFGDNGSGMHTHQSLWKDGSPLMYDETGYAGLSDTARHYIGGLLHHAPSLLAFT
NPTTNSYKRLVPGFEAPINLVYSQRNRSACVRIPITGTNPKAKRLEFRCPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAP
VDKDLYELPPEEAANIPQAPTQLSAVIDRLEEDHEYLTEGGVFTPDLIETWISFKRETEILPVQVRPHPYEFALYYDV

Sequences:

>Translated_478_residues
MTETTPDDVFKLAKDEKVEFVDVRFCDLPGIMQHFTIPVSFFDESVFEDGLAFDGSSIRGFQSIHESDMLLLPDPATAQI
DLFREAKTLNLNFFVHDPFTLEPYSRDPRNIARKAENYLISTGVADTAYFGAEAEFYIFDSVSFDSRTNGSFYEIDAISG
WWNTGSPNELDGSPNRGYKVRPKGGYFPVAPVDHYVDLRDQMLSNLIKAGFSLEKGHHEVGTGGQAEINYKFNTLLHAAD
DMMLYKYIVKNTAWQNGKTVTFMPKPLFGDNGSGMHTHQSLWKDGSPLMYDETGYAGLSDTARHYIGGLLHHAPSLLAFT
NPTTNSYKRLVPGFEAPINLVYSQRNRSACVRIPITGTNPKAKRLEFRCPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAP
VDKDLYELPPEEAANIPQAPTQLSAVIDRLEEDHEYLTEGGVFTPDLIETWISFKRETEILPVQVRPHPYEFALYYDV
>Mature_477_residues
TETTPDDVFKLAKDEKVEFVDVRFCDLPGIMQHFTIPVSFFDESVFEDGLAFDGSSIRGFQSIHESDMLLLPDPATAQID
LFREAKTLNLNFFVHDPFTLEPYSRDPRNIARKAENYLISTGVADTAYFGAEAEFYIFDSVSFDSRTNGSFYEIDAISGW
WNTGSPNELDGSPNRGYKVRPKGGYFPVAPVDHYVDLRDQMLSNLIKAGFSLEKGHHEVGTGGQAEINYKFNTLLHAADD
MMLYKYIVKNTAWQNGKTVTFMPKPLFGDNGSGMHTHQSLWKDGSPLMYDETGYAGLSDTARHYIGGLLHHAPSLLAFTN
PTTNSYKRLVPGFEAPINLVYSQRNRSACVRIPITGTNPKAKRLEFRCPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAPV
DKDLYELPPEEAANIPQAPTQLSAVIDRLEEDHEYLTEGGVFTPDLIETWISFKRETEILPVQVRPHPYEFALYYDV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=474, Percent_Identity=51.0548523206751, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI87081870, Length=466, Percent_Identity=25.9656652360515, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53686; Mature: 53555

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETTPDDVFKLAKDEKVEFVDVRFCDLPGIMQHFTIPVSFFDESVFEDGLAFDGSSIRG
CCCCCHHHHHHHHCCCCEEEEEEEEECCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
FQSIHESDMLLLPDPATAQIDLFREAKTLNLNFFVHDPFTLEPYSRDPRNIARKAENYLI
HHHHCCCCEEEECCCCCCEEEEEECCEEEEEEEEEECCEEECCCCCCHHHHHHHHHCEEE
STGVADTAYFGAEAEFYIFDSVSFDSRTNGSFYEIDAISGWWNTGSPNELDGSPNRGYKV
ECCCCCHHEECCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEE
RPKGGYFPVAPVDHYVDLRDQMLSNLIKAGFSLEKGHHEVGTGGQAEINYKFNTLLHAAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
DMMLYKYIVKNTAWQNGKTVTFMPKPLFGDNGSGMHTHQSLWKDGSPLMYDETGYAGLSD
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCHH
TARHYIGGLLHHAPSLLAFTNPTTNSYKRLVPGFEAPINLVYSQRNRSACVRIPITGTNP
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCC
KAKRLEFRCPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAPVDKDLYELPPEEAANIPQAP
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHCCCCCCH
TQLSAVIDRLEEDHEYLTEGGVFTPDLIETWISFKRETEILPVQVRPHPYEFALYYDV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TETTPDDVFKLAKDEKVEFVDVRFCDLPGIMQHFTIPVSFFDESVFEDGLAFDGSSIRG
CCCCHHHHHHHHCCCCEEEEEEEEECCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
FQSIHESDMLLLPDPATAQIDLFREAKTLNLNFFVHDPFTLEPYSRDPRNIARKAENYLI
HHHHCCCCEEEECCCCCCEEEEEECCEEEEEEEEEECCEEECCCCCCHHHHHHHHHCEEE
STGVADTAYFGAEAEFYIFDSVSFDSRTNGSFYEIDAISGWWNTGSPNELDGSPNRGYKV
ECCCCCHHEECCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEE
RPKGGYFPVAPVDHYVDLRDQMLSNLIKAGFSLEKGHHEVGTGGQAEINYKFNTLLHAAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
DMMLYKYIVKNTAWQNGKTVTFMPKPLFGDNGSGMHTHQSLWKDGSPLMYDETGYAGLSD
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCHH
TARHYIGGLLHHAPSLLAFTNPTTNSYKRLVPGFEAPINLVYSQRNRSACVRIPITGTNP
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCC
KAKRLEFRCPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAPVDKDLYELPPEEAANIPQAP
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHCCCCCCH
TQLSAVIDRLEEDHEYLTEGGVFTPDLIETWISFKRETEILPVQVRPHPYEFALYYDV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]