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The map label for this gene is lipA

Identifier: 41408057

GI number: 41408057

Start: 2162795

End: 2163718

Strand: Direct

Name: lipA

Synonym: MAP1959

Alternate gene names: 41408057

Gene position: 2162795-2163718 (Clockwise)

Preceding gene: 41408056

Following gene: 41408058

Centisome position: 44.78

GC content: 71.32

Gene sequence:

>924_bases
GTGACTGTCGCACCCGAGGGACGCAAACTGCTGCGCCTGGAAGTGCGCAACGCCGAGACCCCGATCGAGCGCAAACCGCC
GTGGATCCGGGTGCGGGCCCGGATGGGGCCGGAATACACCGAGCTCAAGAGCCTGGTCCGGCGCGAGGGGCTGCACACCG
TCTGCGAAGAGGCCGGCTGCCCCAACATCTTCGAATGCTGGGAGGACCGCGAGGCCACCTTCCTGATCGGCGGCGACCAG
TGCACCCGTCGCTGCGACTTCTGCCAGATCGACACCGGCAAGCCAGCCGAGCTGGACCGCGACGAGCCCCGCCGGGTGGC
CGACAGCGTGCGCACGATGGGGCTGCGCTACGCCACGGTCACCGGGGTGGCCCGCGACGACCTGCCCGACGGCGGCGCCT
GGCTGTACGCCGAGACGGTGCGCGCCATCAAGGAGCTCAACCCGTCGACCGGTGTCGAGCTGTTGATCCCCGACTTCAAC
GGCCGGCCCGACCGGCTCGCCGAGGTGTTCGGGTCGCGCCCGGAAGTGTTGGCGCACAACGTCGAAACGGTGCCGCGGAT
CTTCAAACGGATCCGCCCGGCCTTCACCTACCGGCGCAGCCTGGACGTGCTGACCGCGGCCCGCGAGGCCGGGCTGGTCA
CCAAGAGCAACCTCATCCTCGGCTTGGGCGAAACCCCCGACGAGGTGCGCACCGCGCTGGCCGACCTGCGCGGCGCCGGC
TGCGACATCATCACCATCACCCAGTACCTGCGCCCGTCGGCCCGCCACCACCCGGTCGAGCGCTGGGTGAAGCCGGAGGA
ATTCGTCGAGTTCGCGCGGCATGCCGAAGAGCTGGGCTTCTCCGGGGTGCTGGCCGGGCCGCTGGTGCGCTCGTCGTACC
GCGCCGGGCGGCTCTACCGGCAGACCGCCCGCGCCCGCGCCTAG

Upstream 100 bases:

>100_bases
ACGACGTCGTGACGTCGGTCGCCGACGCCGTCTGCGACGCCCTGGACGGGGTGCTGCCGGTGCGTGAGCACTCACCCGGC
GCCCGCGTAGCATCGGCGAT

Downstream 100 bases:

>100_bases
CCGCCACGGCGCTACGTATTCTTTGACTATGGCTAAATCCCGCTCTGCCGCCGACAACAAGGCGGCCCGCGCGCAGGCGC
AGGCCGCCCGCAAGGCCGCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTVAPEGRKLLRLEVRNAETPIERKPPWIRVRARMGPEYTELKSLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQ
CTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAETVRAIKELNPSTGVELLIPDFN
GRPDRLAEVFGSRPEVLAHNVETVPRIFKRIRPAFTYRRSLDVLTAAREAGLVTKSNLILGLGETPDEVRTALADLRGAG
CDIITITQYLRPSARHHPVERWVKPEEFVEFARHAEELGFSGVLAGPLVRSSYRAGRLYRQTARARA

Sequences:

>Translated_307_residues
MTVAPEGRKLLRLEVRNAETPIERKPPWIRVRARMGPEYTELKSLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQ
CTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAETVRAIKELNPSTGVELLIPDFN
GRPDRLAEVFGSRPEVLAHNVETVPRIFKRIRPAFTYRRSLDVLTAAREAGLVTKSNLILGLGETPDEVRTALADLRGAG
CDIITITQYLRPSARHHPVERWVKPEEFVEFARHAEELGFSGVLAGPLVRSSYRAGRLYRQTARARA
>Mature_306_residues
TVAPEGRKLLRLEVRNAETPIERKPPWIRVRARMGPEYTELKSLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQC
TRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAETVRAIKELNPSTGVELLIPDFNG
RPDRLAEVFGSRPEVLAHNVETVPRIFKRIRPAFTYRRSLDVLTAAREAGLVTKSNLILGLGETPDEVRTALADLRGAGC
DIITITQYLRPSARHHPVERWVKPEEFVEFARHAEELGFSGVLAGPLVRSSYRAGRLYRQTARARA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=292, Percent_Identity=42.8082191780822, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=43.0894308943089, Blast_Score=195, Evalue=4e-50,
Organism=Escherichia coli, GI1786846, Length=297, Percent_Identity=42.7609427609428, Blast_Score=251, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=42.2145328719723, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=43.7062937062937, Blast_Score=225, Evalue=6e-60,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=45.1048951048951, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34577; Mature: 34446

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAPEGRKLLRLEVRNAETPIERKPPWIRVRARMGPEYTELKSLVRREGLHTVCEEAGC
CCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
PNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATV
CCHHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHE
TGVARDDLPDGGAWLYAETVRAIKELNPSTGVELLIPDFNGRPDRLAEVFGSRPEVLAHN
ECCHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHC
VETVPRIFKRIRPAFTYRRSLDVLTAAREAGLVTKSNLILGLGETPDEVRTALADLRGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHHHHCCCC
CDIITITQYLRPSARHHPVERWVKPEEFVEFARHAEELGFSGVLAGPLVRSSYRAGRLYR
CCCEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QTARARA
HHHHCCC
>Mature Secondary Structure 
TVAPEGRKLLRLEVRNAETPIERKPPWIRVRARMGPEYTELKSLVRREGLHTVCEEAGC
CCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
PNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATV
CCHHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHE
TGVARDDLPDGGAWLYAETVRAIKELNPSTGVELLIPDFNGRPDRLAEVFGSRPEVLAHN
ECCHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHC
VETVPRIFKRIRPAFTYRRSLDVLTAAREAGLVTKSNLILGLGETPDEVRTALADLRGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHHHHCCCC
CDIITITQYLRPSARHHPVERWVKPEEFVEFARHAEELGFSGVLAGPLVRSSYRAGRLYR
CCCEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QTARARA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]