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The map label for this gene is thiI

Identifier: 41407797

GI number: 41407797

Start: 1857846

End: 1859075

Strand: Direct

Name: thiI

Synonym: MAP1699

Alternate gene names: 41407797

Gene position: 1857846-1859075 (Clockwise)

Preceding gene: 41407795

Following gene: 41407804

Centisome position: 38.47

GC content: 71.06

Gene sequence:

>1230_bases
GTGCACATGCAATGCTGTGTTCTGCTCAAGTACGGGGAGATGGTGCTCAAGGGCGGCAACCGGCGCTGGTTCGAGCAGTG
GCTGCTGACCAATCTCGACGACGCACTGTCGGCGTGGCCGCCCGGGCACCGCCCCGCGGTCCGGCGCCGTGGCGGCGTGC
TGGTGTTGTTCACCTCCCCGGCACTGCAGGACGAGTTGGTTGCGTTGGCCCGCAACCTGATCGGCATCAGCCTGGTGCAG
CCGGTCTGGCGGGTGCCCAGGTCCGCTCGGGCTGCCGAAACCGCTGCGGTGGAGTTGCTCCGAGACCACCGCACCCAGGA
CGGGGCGCCGACGTTCGCGGTGCGCTGCCACCGGCGCGACAAGCGGTTCGGGTTGAGCTCGGAGCAATTGGCCGCCCGCA
TCGGCGCCCGCGTCCGCGCCGACCTGGGGTGGCGGGTCGACCTGTCGCACCCGGAGCTGGAATTGACCGTCGAGGTCGAC
CGGCGCGAGATCTTTCTCGGCACCCGAACGCATCCCGGACAGGGCGGCTTGCCGGTGGGGTCCAGCGGCCGGGCGGTGGT
GTTGCTCTCCGGCGGGTTCGATTCGCCGGTGGCGGCATACCGCGCCATGCGGCGCGGACTGGCCTGCGATTTCCTGCACT
GCACCGGCGCCCCGTTCACCGATGCCTCCTCGACGTACAAGGCGTATGCGTTGGTTCGCCAACTCACCCGCTTCCAGCCC
CGATGCCGGCTGTACGTGGCCGCGGTGGGCCGGGCCCAGCGGACCTTGGCGGCCAGCGGCGCCGGGGAAGCCCAGATCGT
CGCCCAGCGGCGGCTGTATCTCCGCCTGGCCTGTGGCCTTGCCGAACGGATCGGCGCCCAGGCCGTCGTGACCGGCGACA
GCCTGGGTCAGGTGGCCAGCCAGACGCTGCCCAACCTGGCCGCCACCGAACAGGCCGCCACCCTGCCGGTGCTGCGGCCG
CTGCTGGGCTTCGACAAGCAGGAAATCCTCGCCGAGGCCCGGCGGATCGGCACCGCCGAGATCGCCATGCTGCCCGACCA
GGACTGCTGCCAGCTGTTCCTGCCGCGCCGGGTGGCCACCCATGCCGCCGTGGACCAACTCCTGCCGGTCGAGGCCCGGG
CCGGCGTCGACACGCTGGTGGGCAACCTGCTGGACCGTATGCAGCGATTCGATTTCGGAACTGAGGCCGCCGGTGTCGAG
TCCGCCGCGACGGCATCCAGGGTGGGCTAA

Upstream 100 bases:

>100_bases
TATTCCTGCGCGGAGACGATCCGCCCGGCCCGGGTGTGGCCCACGCATCGGTCTGGGCGGCGTGGTAGCGGGGTAGTCGG
TAGCCGGAGGTGAACGAGAC

Downstream 100 bases:

>100_bases
TCCGTGCGCCCGGCCAGCCGGTATGCGATCGCGTCGAATGCGCTCATCGTGTCCAGCATGTGTGGCTCGTGGGAATGCGG
CTGGGTGGACACCTTGGCGG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MHMQCCVLLKYGEMVLKGGNRRWFEQWLLTNLDDALSAWPPGHRPAVRRRGGVLVLFTSPALQDELVALARNLIGISLVQ
PVWRVPRSARAAETAAVELLRDHRTQDGAPTFAVRCHRRDKRFGLSSEQLAARIGARVRADLGWRVDLSHPELELTVEVD
RREIFLGTRTHPGQGGLPVGSSGRAVVLLSGGFDSPVAAYRAMRRGLACDFLHCTGAPFTDASSTYKAYALVRQLTRFQP
RCRLYVAAVGRAQRTLAASGAGEAQIVAQRRLYLRLACGLAERIGAQAVVTGDSLGQVASQTLPNLAATEQAATLPVLRP
LLGFDKQEILAEARRIGTAEIAMLPDQDCCQLFLPRRVATHAAVDQLLPVEARAGVDTLVGNLLDRMQRFDFGTEAAGVE
SAATASRVG

Sequences:

>Translated_409_residues
MHMQCCVLLKYGEMVLKGGNRRWFEQWLLTNLDDALSAWPPGHRPAVRRRGGVLVLFTSPALQDELVALARNLIGISLVQ
PVWRVPRSARAAETAAVELLRDHRTQDGAPTFAVRCHRRDKRFGLSSEQLAARIGARVRADLGWRVDLSHPELELTVEVD
RREIFLGTRTHPGQGGLPVGSSGRAVVLLSGGFDSPVAAYRAMRRGLACDFLHCTGAPFTDASSTYKAYALVRQLTRFQP
RCRLYVAAVGRAQRTLAASGAGEAQIVAQRRLYLRLACGLAERIGAQAVVTGDSLGQVASQTLPNLAATEQAATLPVLRP
LLGFDKQEILAEARRIGTAEIAMLPDQDCCQLFLPRRVATHAAVDQLLPVEARAGVDTLVGNLLDRMQRFDFGTEAAGVE
SAATASRVG
>Mature_409_residues
MHMQCCVLLKYGEMVLKGGNRRWFEQWLLTNLDDALSAWPPGHRPAVRRRGGVLVLFTSPALQDELVALARNLIGISLVQ
PVWRVPRSARAAETAAVELLRDHRTQDGAPTFAVRCHRRDKRFGLSSEQLAARIGARVRADLGWRVDLSHPELELTVEVD
RREIFLGTRTHPGQGGLPVGSSGRAVVLLSGGFDSPVAAYRAMRRGLACDFLHCTGAPFTDASSTYKAYALVRQLTRFQP
RCRLYVAAVGRAQRTLAASGAGEAQIVAQRRLYLRLACGLAERIGAQAVVTGDSLGQVASQTLPNLAATEQAATLPVLRP
LLGFDKQEILAEARRIGTAEIAMLPDQDCCQLFLPRRVATHAAVDQLLPVEARAGVDTLVGNLLDRMQRFDFGTEAAGVE
SAATASRVG

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=256, Percent_Identity=32.8125, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 44455; Mature: 44455

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMQCCVLLKYGEMVLKGGNRRWFEQWLLTNLDDALSAWPPGHRPAVRRRGGVLVLFTSP
CCCEEEEHHHHCHHHEECCCHHHHHHHHHHCHHHHHHCCCCCCCCHHHHCCCEEEEEECC
ALQDELVALARNLIGISLVQPVWRVPRSARAAETAAVELLRDHRTQDGAPTFAVRCHRRD
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
KRFGLSSEQLAARIGARVRADLGWRVDLSHPELELTVEVDRREIFLGTRTHPGQGGLPVG
HHCCCCHHHHHHHHCCHHEECCCEEEECCCCCEEEEEEECCEEEEEECCCCCCCCCCCCC
SSGRAVVLLSGGFDSPVAAYRAMRRGLACDFLHCTGAPFTDASSTYKAYALVRQLTRFQP
CCCCEEEEEECCCCCHHHHHHHHHCCCCEEEHCCCCCCCCCCCHHHHHHHHHHHHHCCCC
RCRLYVAAVGRAQRTLAASGAGEAQIVAQRRLYLRLACGLAERIGAQAVVTGDSLGQVAS
HHEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHH
QTLPNLAATEQAATLPVLRPLLGFDKQEILAEARRIGTAEIAMLPDQDCCQLFLPRRVAT
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
HAAVDQLLPVEARAGVDTLVGNLLDRMQRFDFGTEAAGVESAATASRVG
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MHMQCCVLLKYGEMVLKGGNRRWFEQWLLTNLDDALSAWPPGHRPAVRRRGGVLVLFTSP
CCCEEEEHHHHCHHHEECCCHHHHHHHHHHCHHHHHHCCCCCCCCHHHHCCCEEEEEECC
ALQDELVALARNLIGISLVQPVWRVPRSARAAETAAVELLRDHRTQDGAPTFAVRCHRRD
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
KRFGLSSEQLAARIGARVRADLGWRVDLSHPELELTVEVDRREIFLGTRTHPGQGGLPVG
HHCCCCHHHHHHHHCCHHEECCCEEEECCCCCEEEEEEECCEEEEEECCCCCCCCCCCCC
SSGRAVVLLSGGFDSPVAAYRAMRRGLACDFLHCTGAPFTDASSTYKAYALVRQLTRFQP
CCCCEEEEEECCCCCHHHHHHHHHCCCCEEEHCCCCCCCCCCCHHHHHHHHHHHHHCCCC
RCRLYVAAVGRAQRTLAASGAGEAQIVAQRRLYLRLACGLAERIGAQAVVTGDSLGQVAS
HHEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHH
QTLPNLAATEQAATLPVLRPLLGFDKQEILAEARRIGTAEIAMLPDQDCCQLFLPRRVAT
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
HAAVDQLLPVEARAGVDTLVGNLLDRMQRFDFGTEAAGVESAATASRVG
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA