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The map label for this gene is gcvH

Identifier: 41407637

GI number: 41407637

Start: 1689843

End: 1690241

Strand: Direct

Name: gcvH

Synonym: MAP1539

Alternate gene names: 41407637

Gene position: 1689843-1690241 (Clockwise)

Preceding gene: 41407636

Following gene: 41407638

Centisome position: 34.99

GC content: 66.92

Gene sequence:

>399_bases
GTGAGCGACATCCCGCCCGACCTGCACTACACCGCCGAACACGAATGGGTTCGCCGAAGCGGTGATGACACCGTGCGGGT
GGGCATCACCGACTTCGCCCAGTCGGCGTTGGGTGACGTCGTTTTCGTTCAGCTGCCCGAGGTGGGCGCGCAGCTGACCG
CCGGGGAGTCCTTCGGCGAGGTCGAATCCACGAAATCGGTGTCGGACCTCTACGCCCCGGTGTCGGGCACGGTGACGGCG
GTCAACACCGACCTGGAAGGCAGCCCGCAGCTGGTCAACTCCGACCCGTACGGGGCCGGCTGGTTGCTGGATGTCCAGGT
GTCCGACGCCGCCGCCTTGGAGTCGGCCATTACCGCATTGCTCGACGCCGAGGCCTACCGCGGAACGCTGACCGAATGA

Upstream 100 bases:

>100_bases
AGCAGGCCGACCGGGTGGACGTGACCACCTTGCGGCAACCAAAACCGCACCAATACGCTCAGCCCGTCAAGTGAGCAACT
CCCGAACAGACAGGATCACC

Downstream 100 bases:

>100_bases
CGGTTGCTAATGTCCCTGCAGCCCGCCCGGCCAAGCGAGCGGTGCGGCCGGCACACGGGGGAAATCAACGTTTCGGATCG
TCAGGTGGTGGGCGCATTGC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSDIPPDLHYTAEHEWVRRSGDDTVRVGITDFAQSALGDVVFVQLPEVGAQLTAGESFGEVESTKSVSDLYAPVSGTVTA
VNTDLEGSPQLVNSDPYGAGWLLDVQVSDAAALESAITALLDAEAYRGTLTE

Sequences:

>Translated_132_residues
MSDIPPDLHYTAEHEWVRRSGDDTVRVGITDFAQSALGDVVFVQLPEVGAQLTAGESFGEVESTKSVSDLYAPVSGTVTA
VNTDLEGSPQLVNSDPYGAGWLLDVQVSDAAALESAITALLDAEAYRGTLTE
>Mature_131_residues
SDIPPDLHYTAEHEWVRRSGDDTVRVGITDFAQSALGDVVFVQLPEVGAQLTAGESFGEVESTKSVSDLYAPVSGTVTAV
NTDLEGSPQLVNSDPYGAGWLLDVQVSDAAALESAITALLDAEAYRGTLTE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=45.3781512605042, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=44.5378151260504, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789271, Length=130, Percent_Identity=53.0769230769231, Blast_Score=142, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=49.1525423728814, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=109, Percent_Identity=44.954128440367, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=43.6507936507937, Blast_Score=120, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17865652, Length=130, Percent_Identity=41.5384615384615, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_MYCA1 (A0QGN7)

Other databases:

- EMBL:   CP000479
- RefSeq:   YP_882075.1
- ProteinModelPortal:   A0QGN7
- SMR:   A0QGN7
- STRING:   A0QGN7
- EnsemblBacteria:   EBMYCT00000011900
- GeneID:   4529614
- GenomeReviews:   CP000479_GR
- KEGG:   mav:MAV_2889
- TIGR:   MAV_2889
- eggNOG:   COG0509
- GeneTree:   EBGT00050000015839
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- PhylomeDB:   A0QGN7
- ProtClustDB:   PRK01202
- BioCyc:   MAVI243243:MAV_2889-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13915; Mature: 13784

Theoretical pI: Translated: 3.70; Mature: 3.70

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIPPDLHYTAEHEWVRRSGDDTVRVGITDFAQSALGDVVFVQLPEVGAQLTAGESFGE
CCCCCCCCEECCCCHHHHCCCCCEEEECHHHHHHHHHCCEEEEECCCCCCEEECCCCCCC
VESTKSVSDLYAPVSGTVTAVNTDLEGSPQLVNSDPYGAGWLLDVQVSDAAALESAITAL
HHCCCCHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCEEEEEEECCHHHHHHHHHHH
LDAEAYRGTLTE
HHHHHHCCCCCC
>Mature Secondary Structure 
SDIPPDLHYTAEHEWVRRSGDDTVRVGITDFAQSALGDVVFVQLPEVGAQLTAGESFGE
CCCCCCCEECCCCHHHHCCCCCEEEECHHHHHHHHHCCEEEEECCCCCCEEECCCCCCC
VESTKSVSDLYAPVSGTVTAVNTDLEGSPQLVNSDPYGAGWLLDVQVSDAAALESAITAL
HHCCCCHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCEEEEEEECCHHHHHHHHHHH
LDAEAYRGTLTE
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA