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The map label for this gene is pyrB

Identifier: 41407213

GI number: 41407213

Start: 1167190

End: 1168146

Strand: Direct

Name: pyrB

Synonym: MAP1115

Alternate gene names: 41407213

Gene position: 1167190-1168146 (Clockwise)

Preceding gene: 41407212

Following gene: 41407214

Centisome position: 24.17

GC content: 71.37

Gene sequence:

>957_bases
ATGACCCGCCACCTGCTCGCCGCCGGCGACCTGAGCCGCGACGACGCCACCGCCATCCTCGACGACGCCGACCGGTTCGC
CCAGGCGCTGGTGGGCCGCGAGATCAAGAAACTGCCGACGCTGCGTGGCCGCACCGTCGTCACCATGTTCTACGAGAACT
CCACCCGCACCCGGGTGTCCTTCGAGGTGGCCGGCAAGTGGATGAGCGCCGACGTGATCAACGTCAGCGCCGCCGGGTCC
TCGGTGGGCAAGGGGGAGTCGCTGCGCGACACCGCCCTGACGCTGCGGGCGGCCGGCGCCGACGCGCTGATCATCCGGCA
CCCGGCCTCCGGCGCCGCGCACCTGCTTGCCGAATGGACTTGCGGCAACGGCGATTCCAGCGACGCCGGGCCGTCGGTGA
TCAACGCCGGCGACGGCACCCACGAGCACCCGACGCAGGCGCTGCTGGACGCGCTGACCATCCGCCAGCGGCTCGGCGGC
ATCGAGGGCCGGCGCGTGGTGATCGTCGGCGACGTCCTGCACAGCCGGGTGGCCCGCTCCAACGTGATGCTGCTGTCCAC
CCTGGGCGCCGAGGTGGTGCTGGTCGCCCCGCCGACGCTGTTGCCGGTCGGCGTCGAGGGCTGGCCGGTGACCGTGTCGA
ACGACTTCGACGCCGAGCTGCCCGCCGCCGATGCGGTGCTCATGCTGCGCGTGCAGGCCGAGCGGATGAACGGTGGCTTC
TTCCCGTCGGTGCGCGAGTACTCGTCCCTGTACGGGCTGTCCGATCGCCGCCAGGCGATGCTGCCTGGCCACGCCGTGGT
GCTGCACCCGGGCCCGATGCTGCGCGGCATGGAGATCGCCTCCTCGGTGGCGGACTCGTCGCAATCGGCTGTGCTGCAAC
AGGTTTCCAATGGTGTGCACGTGCGGATGGCGGTGCTGTTCCACGTGCTGGTGGGCGCGGAGGAGATCGCCGCATGA

Upstream 100 bases:

>100_bases
GCGCCGACTACGTCGGCAAGAACGTCCCCACCTCGCGCAGTGAGAGCGTGCACGTGCTGCTGGCCGAGCACGACGGCGCC
GACGGGGTGGTGATCTCGCG

Downstream 100 bases:

>100_bases
GCGTGTTGCTGCGCGGAGTCCGGTTGTACGGCGAGGGCGACCGGGTCGATGTCCTGGTCGACGACGGGCAGATCGCCGAC
ATCGGCGCCGGGCTCGACAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTRHLLAAGDLSRDDATAILDDADRFAQALVGREIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAGS
SVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAEWTCGNGDSSDAGPSVINAGDGTHEHPTQALLDALTIRQRLGG
IEGRRVVIVGDVLHSRVARSNVMLLSTLGAEVVLVAPPTLLPVGVEGWPVTVSNDFDAELPAADAVLMLRVQAERMNGGF
FPSVREYSSLYGLSDRRQAMLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGAEEIAA

Sequences:

>Translated_318_residues
MTRHLLAAGDLSRDDATAILDDADRFAQALVGREIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAGS
SVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAEWTCGNGDSSDAGPSVINAGDGTHEHPTQALLDALTIRQRLGG
IEGRRVVIVGDVLHSRVARSNVMLLSTLGAEVVLVAPPTLLPVGVEGWPVTVSNDFDAELPAADAVLMLRVQAERMNGGF
FPSVREYSSLYGLSDRRQAMLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGAEEIAA
>Mature_317_residues
TRHLLAAGDLSRDDATAILDDADRFAQALVGREIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAGSS
VGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAEWTCGNGDSSDAGPSVINAGDGTHEHPTQALLDALTIRQRLGGI
EGRRVVIVGDVLHSRVARSNVMLLSTLGAEVVLVAPPTLLPVGVEGWPVTVSNDFDAELPAADAVLMLRVQAERMNGGFF
PSVREYSSLYGLSDRRQAMLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGAEEIAA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=318, Percent_Identity=36.1635220125786, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI2367364, Length=321, Percent_Identity=33.0218068535826, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786469, Length=346, Percent_Identity=26.878612716763, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790703, Length=346, Percent_Identity=27.1676300578035, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=32.8358208955224, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=324, Percent_Identity=33.0246913580247, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24642586, Length=325, Percent_Identity=33.5384615384615, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33594; Mature: 33463

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHLLAAGDLSRDDATAILDDADRFAQALVGREIKKLPTLRGRTVVTMFYENSTRTRVS
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEE
FEVAGKWMSADVINVSAAGSSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAEWT
EEECCCEEECCEEEEECCCCCCCCCCCCHHHEEEEEECCCCEEEEECCCCCCEEEEEEEE
CGNGDSSDAGPSVINAGDGTHEHPTQALLDALTIRQRLGGIEGRRVVIVGDVLHSRVARS
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHC
NVMLLSTLGAEVVLVAPPTLLPVGVEGWPVTVSNDFDAELPAADAVLMLRVQAERMNGGF
CEEEEEECCCCEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHCCCCC
FPSVREYSSLYGLSDRRQAMLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVH
CCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCC
VRMAVLFHVLVGAEEIAA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRHLLAAGDLSRDDATAILDDADRFAQALVGREIKKLPTLRGRTVVTMFYENSTRTRVS
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEE
FEVAGKWMSADVINVSAAGSSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAEWT
EEECCCEEECCEEEEECCCCCCCCCCCCHHHEEEEEECCCCEEEEECCCCCCEEEEEEEE
CGNGDSSDAGPSVINAGDGTHEHPTQALLDALTIRQRLGGIEGRRVVIVGDVLHSRVARS
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHC
NVMLLSTLGAEVVLVAPPTLLPVGVEGWPVTVSNDFDAELPAADAVLMLRVQAERMNGGF
CEEEEEECCCCEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHCCCCC
FPSVREYSSLYGLSDRRQAMLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVH
CCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCC
VRMAVLFHVLVGAEEIAA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA