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The map label for this gene is kstD [H]

Identifier: 41406794

GI number: 41406794

Start: 717556

End: 719283

Strand: Direct

Name: kstD [H]

Synonym: MAP0696

Alternate gene names: 41406794

Gene position: 717556-719283 (Clockwise)

Preceding gene: 41406793

Following gene: 41406795

Centisome position: 14.86

GC content: 68.58

Gene sequence:

>1728_bases
ATGACCGATTTCGACCACGTCGTCGACGTACTCATCGTCGGTTCGGGCGGCGGCGGGATGACGGCCGCGCTCACCGCCCA
GGCCGCCGGGCTGGATGCGCTGATCGTCGAAAAGTCCTCCCACTTCGGGGGTTCCACCGCGCTGTCCGGAGGCGGCATCT
GGGTGCCCGGCGCGCCGGCGCAGCGGCGGGAGGGCTACTCGCCCTCGCCGGAAGGGGTGGTGGAATACCTCAAACACATC
ACCGACGGGCTGGTCAGCGAGGCGCGGATACGGCAATACGTCGAGACCGCGCCGCAGATGCTGGCATTCTTGGAAAACCT
CTCCGGATGGTTCGAATTCGTCTGGAAGCCCGGGTATGCCGACTACTATCCCGAGCTGCCCGGCGGCTCCGAACTCGGCA
GCACCATCAACGTGCCACCCATCGACCTGCGCAAGCTCGGGCCCGACGAGCACACGCTGCTGCAGCCGCTGGCCCTGGCC
CCCAAGGGAATCTGGCTGGGCCCCAAGGAGTTACGCACCTTCTACCGGATCCGGCAGTCCTGGGCGGGCAAAGCCGTGCT
GCTCAAGCTGATCGCCCGGATGGTCAGGGCACGGGTGTTCGGCGAGCGGATGGCCGCGATCGGGCAGTCGCTCGCCGCCC
GGATGCGGCTGGCGCTGCGCGACCGCGGTGTCCCGCTGTGGCTGGACTCGCCGATGGTGCGGTTGCTCACCGACCCGCAG
GGAGCGGTCAGCGGCGCGGTGGTGGAACGCGAAGGCAAGCCGCAGCGCATCGGCGCACGGTTCGGCGTCATCCTGGCCTC
CGGTGGCTTCGACCACGACCTCGCCTGGCGCAAGGAGCTACTGCCCGAGGTGGACCAGGACTGGAGCTTCGGCAACCCGG
CCGCGATGGGGGACGGCATCCGGGCCGGCCAAAACGTGGGCGCCGCAACGGACTTGCTCGACGAGGCGTGGTGGTTCCCG
GCCATCCAATGGCCGGACGGGCGCATGCAATTCATGCTCAACGAGCGGATGATGCCGGCGCAATTCATCGTCAACGGCGC
CGGCCGACGGTTCATCAACGAGGCGGCCCCGTACATGGACTTCGGCCACGCCATGATCGAGGGCCAGCGGTCCGGTGTCA
CCCACATCCCGTGCTGGCTGATCACCGACCACAGGTCGTGGAACCGCTACGTCATCGGCGGGCACCTGCCGCTGCCGAAG
ATCCCCGGGGCGCCGGTGCCCACCGGCCGCAAGATCCCGCCGGCCTGGCTGGAATCGGGTGTCGTCAAGGCCGCGATGAC
GTGGGACGAGATGGCGGCGCAGATCGGGGTGCCCGCCGGGGAGCTCGCCGAAACCGCGCGACGGTTCAACGAACTCGCCC
GCAAGGGCCACGACGACGATTTCAACCGCGGCGACAGCGTCTACGACAACTACTACGGCGACCCGACCTTGCCCAACCCC
AACCTGTATCCGCTGGGTGATCCGCCGTATTACGCCTTCCGAATCGTGCTCGGGGACCTCGGAACCTCGGGCGGCCTGCG
CACCGACGAACACGCCCGCGTGCTGCGGGCCGACGACACCGTGGTGCCGGGACTCTACGCGGTGGGTAACACCTCCGCGC
CGGTGATGGGCCGCAGCTATGCCGGTGCGGGGGCCACCATCGGCCCCGCCATGACCTTCGGCTACGTCGCGGCGAAACAC
GTTGCGGCCCAAGTCGCCAACCGAGCCGCTAATACTCATAGGAGGTAG

Upstream 100 bases:

>100_bases
CCGCACTGCAGGCGGCCGGTCCCTACGAGACGACCATGCGCTACTACACGCCCGCCCCCGAACTGATCGCCGGATTCGCC
ATCAGAACGGCAAGACCGAA

Downstream 100 bases:

>100_bases
CACATGAAAATCTCGCTGTTCTACGAATTCGCCCTGCCGCGGCCCTGGGCCCCCGACGACGAGCACGTCCTGCTGCAGGA
ATGCCTGGACGAGGTGGAGG

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTDFDHVVDVLIVGSGGGGMTAALTAQAAGLDALIVEKSSHFGGSTALSGGGIWVPGAPAQRREGYSPSPEGVVEYLKHI
TDGLVSEARIRQYVETAPQMLAFLENLSGWFEFVWKPGYADYYPELPGGSELGSTINVPPIDLRKLGPDEHTLLQPLALA
PKGIWLGPKELRTFYRIRQSWAGKAVLLKLIARMVRARVFGERMAAIGQSLAARMRLALRDRGVPLWLDSPMVRLLTDPQ
GAVSGAVVEREGKPQRIGARFGVILASGGFDHDLAWRKELLPEVDQDWSFGNPAAMGDGIRAGQNVGAATDLLDEAWWFP
AIQWPDGRMQFMLNERMMPAQFIVNGAGRRFINEAAPYMDFGHAMIEGQRSGVTHIPCWLITDHRSWNRYVIGGHLPLPK
IPGAPVPTGRKIPPAWLESGVVKAAMTWDEMAAQIGVPAGELAETARRFNELARKGHDDDFNRGDSVYDNYYGDPTLPNP
NLYPLGDPPYYAFRIVLGDLGTSGGLRTDEHARVLRADDTVVPGLYAVGNTSAPVMGRSYAGAGATIGPAMTFGYVAAKH
VAAQVANRAANTHRR

Sequences:

>Translated_575_residues
MTDFDHVVDVLIVGSGGGGMTAALTAQAAGLDALIVEKSSHFGGSTALSGGGIWVPGAPAQRREGYSPSPEGVVEYLKHI
TDGLVSEARIRQYVETAPQMLAFLENLSGWFEFVWKPGYADYYPELPGGSELGSTINVPPIDLRKLGPDEHTLLQPLALA
PKGIWLGPKELRTFYRIRQSWAGKAVLLKLIARMVRARVFGERMAAIGQSLAARMRLALRDRGVPLWLDSPMVRLLTDPQ
GAVSGAVVEREGKPQRIGARFGVILASGGFDHDLAWRKELLPEVDQDWSFGNPAAMGDGIRAGQNVGAATDLLDEAWWFP
AIQWPDGRMQFMLNERMMPAQFIVNGAGRRFINEAAPYMDFGHAMIEGQRSGVTHIPCWLITDHRSWNRYVIGGHLPLPK
IPGAPVPTGRKIPPAWLESGVVKAAMTWDEMAAQIGVPAGELAETARRFNELARKGHDDDFNRGDSVYDNYYGDPTLPNP
NLYPLGDPPYYAFRIVLGDLGTSGGLRTDEHARVLRADDTVVPGLYAVGNTSAPVMGRSYAGAGATIGPAMTFGYVAAKH
VAAQVANRAANTHRR
>Mature_574_residues
TDFDHVVDVLIVGSGGGGMTAALTAQAAGLDALIVEKSSHFGGSTALSGGGIWVPGAPAQRREGYSPSPEGVVEYLKHIT
DGLVSEARIRQYVETAPQMLAFLENLSGWFEFVWKPGYADYYPELPGGSELGSTINVPPIDLRKLGPDEHTLLQPLALAP
KGIWLGPKELRTFYRIRQSWAGKAVLLKLIARMVRARVFGERMAAIGQSLAARMRLALRDRGVPLWLDSPMVRLLTDPQG
AVSGAVVEREGKPQRIGARFGVILASGGFDHDLAWRKELLPEVDQDWSFGNPAAMGDGIRAGQNVGAATDLLDEAWWFPA
IQWPDGRMQFMLNERMMPAQFIVNGAGRRFINEAAPYMDFGHAMIEGQRSGVTHIPCWLITDHRSWNRYVIGGHLPLPKI
PGAPVPTGRKIPPAWLESGVVKAAMTWDEMAAQIGVPAGELAETARRFNELARKGHDDDFNRGDSVYDNYYGDPTLPNPN
LYPLGDPPYYAFRIVLGDLGTSGGLRTDEHARVLRADDTVVPGLYAVGNTSAPVMGRSYAGAGATIGPAMTFGYVAAKHV
AAQVANRAANTHRR

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 62422; Mature: 62291

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFDHVVDVLIVGSGGGGMTAALTAQAAGLDALIVEKSSHFGGSTALSGGGIWVPGAPA
CCCHHHEEEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCEECCCCEECCCCCH
QRREGYSPSPEGVVEYLKHITDGLVSEARIRQYVETAPQMLAFLENLSGWFEFVWKPGYA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DYYPELPGGSELGSTINVPPIDLRKLGPDEHTLLQPLALAPKGIWLGPKELRTFYRIRQS
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
WAGKAVLLKLIARMVRARVFGERMAAIGQSLAARMRLALRDRGVPLWLDSPMVRLLTDPQ
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCC
GAVSGAVVEREGKPQRIGARFGVILASGGFDHDLAWRKELLPEVDQDWSFGNPAAMGDGI
CHHCCCEEECCCCCHHHCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RAGQNVGAATDLLDEAWWFPAIQWPDGRMQFMLNERMMPAQFIVNGAGRRFINEAAPYMD
CCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCHHH
FGHAMIEGQRSGVTHIPCWLITDHRSWNRYVIGGHLPLPKIPGAPVPTGRKIPPAWLESG
HHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCC
VVKAAMTWDEMAAQIGVPAGELAETARRFNELARKGHDDDFNRGDSVYDNYYGDPTLPNP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCC
NLYPLGDPPYYAFRIVLGDLGTSGGLRTDEHARVLRADDTVVPGLYAVGNTSAPVMGRSY
CCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHEEECCCCCCCCEEEECCCCCCCCCCCC
AGAGATIGPAMTFGYVAAKHVAAQVANRAANTHRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDFDHVVDVLIVGSGGGGMTAALTAQAAGLDALIVEKSSHFGGSTALSGGGIWVPGAPA
CCHHHEEEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCEECCCCEECCCCCH
QRREGYSPSPEGVVEYLKHITDGLVSEARIRQYVETAPQMLAFLENLSGWFEFVWKPGYA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DYYPELPGGSELGSTINVPPIDLRKLGPDEHTLLQPLALAPKGIWLGPKELRTFYRIRQS
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
WAGKAVLLKLIARMVRARVFGERMAAIGQSLAARMRLALRDRGVPLWLDSPMVRLLTDPQ
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCC
GAVSGAVVEREGKPQRIGARFGVILASGGFDHDLAWRKELLPEVDQDWSFGNPAAMGDGI
CHHCCCEEECCCCCHHHCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RAGQNVGAATDLLDEAWWFPAIQWPDGRMQFMLNERMMPAQFIVNGAGRRFINEAAPYMD
CCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCHHH
FGHAMIEGQRSGVTHIPCWLITDHRSWNRYVIGGHLPLPKIPGAPVPTGRKIPPAWLESG
HHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCC
VVKAAMTWDEMAAQIGVPAGELAETARRFNELARKGHDDDFNRGDSVYDNYYGDPTLPNP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCC
NLYPLGDPPYYAFRIVLGDLGTSGGLRTDEHARVLRADDTVVPGLYAVGNTSAPVMGRSY
CCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHEEECCCCCCCCEEEECCCCCCCCCCCC
AGAGATIGPAMTFGYVAAKHVAAQVANRAANTHRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]