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The map label for this gene is phoS2_1

Identifier: 41406749

GI number: 41406749

Start: 672254

End: 673351

Strand: Direct

Name: phoS2_1

Synonym: MAP0651

Alternate gene names: 41406749

Gene position: 672254-673351 (Clockwise)

Preceding gene: 41406748

Following gene: 41406750

Centisome position: 13.92

GC content: 69.67

Gene sequence:

>1098_bases
GTGAGGCTGGACAGGCAGGGCGGGGCGCTGGCCGCCGCGGCGCTGACCGGCTGCGGGAGCGACGAGAACCACCGCGGGAC
CGCCGCGCCGAGCATCAGCGGAACCACCGGCACCGCGGGCTGCGGCGGCAAGAACAAGCTGACCGCGGAGGGCTCGACCG
CGCAGGAAAACGCGATCACGATGTTCAACCAGGTGTGGGGCCAGTACTGCCCGGGCAAGGGCCTGGCCTACAACCCGACC
GGGTCGGGCGCCGGCCGCGAGCAGTTCATCGCCGGGCACGTGGATTTCGCGGGCGCGGACTCGCCGTTGGTCGCCGACCA
GATCGGCCCGGCCGCCCAGCGCTGCGGCGGCAACCCGGCCTGGGACCTGCCGCTGGTGTTCGGGCCGATCGCGATCACCT
ACAACCTGCCCGGCAACCCGGCCCTGGTGGTCAGCAGTGACGCGGTGGCCAAGATCTTCACCGGCAAGATCACCAACTGG
AACGACCCGATCCTGGCGGCCCTCAATCCCGGGGTGGCCCTTCCCGACACCAAGATCACCCCGATCTACCGGACCGACTC
GTCGGGAACCACCGATAACGTGCAGAAGTATCTGACCGCCGCCGCGCCGCAGAGCTGGGCCAAGGGCGTCGGCACCGAAT
TCCAGGGCGGCGTCGGCGAGGGCGCCGCGAAGTCGGCGGGAGTCATCCAGGCGGTGCGGGCCACCACCGGGGCCGTCGGC
TACGTGGAGAAGGGCTTCGCCGACCAGGCCGGCATGCCCTACGCCAAGATCGCCACCCGCGGTGGGGTGGTCCCGCTGAC
CAACGAGACCGCCGGAAACGCCGTCAACGCGGCCAAATTCCTGTCCGAGGGCGACGACCTGGTGCTCGACCTCCACGCCA
TGTACGCCTCCCAGGAGCCGGGCGTCTACCCGTTGCTGCTGGTGACGTACGAGATCGTGTGTTCGAAGGGGTACGACCCC
GAGACGCTGGCGGCCGTGAAATCGTTTCTCGGCGTGGCCGCCACCAGCGGGCAGAACGGCCTTTCCACCGCCGGCTACAT
CCCGCTGCCCGATAAGGTCAGGCAACGACTGGTGACCGCGATCAATGCGTTGCAGTAG

Upstream 100 bases:

>100_bases
GTGCGCCGCGTCGGCGCATATTTTCGCCGCGAGTTGGCAACCTGACAGTCATGCCCGCGGCGCCCGCACGCGTCGGTGAA
CGTGTCAAGAAAGCGAGATC

Downstream 100 bases:

>100_bases
CCGGCGGGCCGGGGTTTCAGGGAGCGACGGCAGGACTGTGGTGGGCGCACCGTGACGGGGTTTTCCGGGCCGAATCCCAC
CGACGCGGGCTCGGGCGCGG

Product: PhoS2_1

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 3; PstS-3 [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MRLDRQGGALAAAALTGCGSDENHRGTAAPSISGTTGTAGCGGKNKLTAEGSTAQENAITMFNQVWGQYCPGKGLAYNPT
GSGAGREQFIAGHVDFAGADSPLVADQIGPAAQRCGGNPAWDLPLVFGPIAITYNLPGNPALVVSSDAVAKIFTGKITNW
NDPILAALNPGVALPDTKITPIYRTDSSGTTDNVQKYLTAAAPQSWAKGVGTEFQGGVGEGAAKSAGVIQAVRATTGAVG
YVEKGFADQAGMPYAKIATRGGVVPLTNETAGNAVNAAKFLSEGDDLVLDLHAMYASQEPGVYPLLLVTYEIVCSKGYDP
ETLAAVKSFLGVAATSGQNGLSTAGYIPLPDKVRQRLVTAINALQ

Sequences:

>Translated_365_residues
MRLDRQGGALAAAALTGCGSDENHRGTAAPSISGTTGTAGCGGKNKLTAEGSTAQENAITMFNQVWGQYCPGKGLAYNPT
GSGAGREQFIAGHVDFAGADSPLVADQIGPAAQRCGGNPAWDLPLVFGPIAITYNLPGNPALVVSSDAVAKIFTGKITNW
NDPILAALNPGVALPDTKITPIYRTDSSGTTDNVQKYLTAAAPQSWAKGVGTEFQGGVGEGAAKSAGVIQAVRATTGAVG
YVEKGFADQAGMPYAKIATRGGVVPLTNETAGNAVNAAKFLSEGDDLVLDLHAMYASQEPGVYPLLLVTYEIVCSKGYDP
ETLAAVKSFLGVAATSGQNGLSTAGYIPLPDKVRQRLVTAINALQ
>Mature_365_residues
MRLDRQGGALAAAALTGCGSDENHRGTAAPSISGTTGTAGCGGKNKLTAEGSTAQENAITMFNQVWGQYCPGKGLAYNPT
GSGAGREQFIAGHVDFAGADSPLVADQIGPAAQRCGGNPAWDLPLVFGPIAITYNLPGNPALVVSSDAVAKIFTGKITNW
NDPILAALNPGVALPDTKITPIYRTDSSGTTDNVQKYLTAAAPQSWAKGVGTEFQGGVGEGAAKSAGVIQAVRATTGAVG
YVEKGFADQAGMPYAKIATRGGVVPLTNETAGNAVNAAKFLSEGDDLVLDLHAMYASQEPGVYPLLLVTYEIVCSKGYDP
ETLAAVKSFLGVAATSGQNGLSTAGYIPLPDKVRQRLVTAINALQ

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=298, Percent_Identity=29.8657718120805, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37247; Mature: 37247

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRQGGALAAAALTGCGSDENHRGTAAPSISGTTGTAGCGGKNKLTAEGSTAQENAIT
CCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
MFNQVWGQYCPGKGLAYNPTGSGAGREQFIAGHVDFAGADSPLVADQIGPAAQRCGGNPA
HHHHHHHCCCCCCCEEECCCCCCCCCHHEEEECCCCCCCCCCCHHHHCCHHHHHCCCCCC
WDLPLVFGPIAITYNLPGNPALVVSSDAVAKIFTGKITNWNDPILAALNPGVALPDTKIT
CCCCEEEEEEEEEEECCCCCEEEEECCCEEEHHCCCCCCCCCCEEEEECCCCCCCCCCCC
PIYRTDSSGTTDNVQKYLTAAAPQSWAKGVGTEFQGGVGEGAAKSAGVIQAVRATTGAVG
EEEECCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHH
YVEKGFADQAGMPYAKIATRGGVVPLTNETAGNAVNAAKFLSEGDDLVLDLHAMYASQEP
HHHHCCCCCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHCCCCC
GVYPLLLVTYEIVCSKGYDPETLAAVKSFLGVAATSGQNGLSTAGYIPLPDKVRQRLVTA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHH
INALQ
HHHCC
>Mature Secondary Structure
MRLDRQGGALAAAALTGCGSDENHRGTAAPSISGTTGTAGCGGKNKLTAEGSTAQENAIT
CCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
MFNQVWGQYCPGKGLAYNPTGSGAGREQFIAGHVDFAGADSPLVADQIGPAAQRCGGNPA
HHHHHHHCCCCCCCEEECCCCCCCCCHHEEEECCCCCCCCCCCHHHHCCHHHHHCCCCCC
WDLPLVFGPIAITYNLPGNPALVVSSDAVAKIFTGKITNWNDPILAALNPGVALPDTKIT
CCCCEEEEEEEEEEECCCCCEEEEECCCEEEHHCCCCCCCCCCEEEEECCCCCCCCCCCC
PIYRTDSSGTTDNVQKYLTAAAPQSWAKGVGTEFQGGVGEGAAKSAGVIQAVRATTGAVG
EEEECCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHH
YVEKGFADQAGMPYAKIATRGGVVPLTNETAGNAVNAAKFLSEGDDLVLDLHAMYASQEP
HHHHCCCCCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHCCCCC
GVYPLLLVTYEIVCSKGYDPETLAAVKSFLGVAATSGQNGLSTAGYIPLPDKVRQRLVTA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHH
INALQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA