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The map label for this gene is purF

Identifier: 41406736

GI number: 41406736

Start: 660721

End: 662376

Strand: Direct

Name: purF

Synonym: MAP0638

Alternate gene names: 41406736

Gene position: 660721-662376 (Clockwise)

Preceding gene: 41406735

Following gene: 41406737

Centisome position: 13.68

GC content: 70.17

Gene sequence:

>1656_bases
CTGAAGTGCAGGTTTACCCCGGCGGGCCGGGATTTCGCCGGTCAACACGCCCGGCGGATTCGGCGGCGCGGCCGCGCTGC
CCGTAGACTCCGTTACGTCACCAACCGTTCCCCGGGGAGCCGCCCAATCGTGACCGTCGCCGAACCCGAGCAGGACTTCA
ACGCACCCCGCGAGGAATGCGGTGTATTCGGGGTCTGGGCCCCGGGCGAGGAAGTCGCCAAACTCACCTACTACGGCCTC
TACGCGCTGCAGCATCGCGGGCAGGAGGCCGCCGGCATCGCCGTCGCCGACGGATCCCAAGTGCTGGTGTTCAAGGACCT
GGGCCTGGTCAGCCAGGTGTTCGACGAGCAGACGCTGGCCGCGATGCAGGGCCACGTCGCCATCGGGCACTGCCGCTACT
CCACCACCGGCGACACCACCTGGGAGAACGCCCAGCCGGTGTTCCGCAACACCGCCGCGGGCACCGGGGTTGCCTTGGGG
CACAACGGAAATCTGGTCAACACCGCCGAGCTGGCCGCGCGGGCCCGCGACGCGGGGCTGATCGCCAAGCGCGCGCCGGC
CCCGGCGACCACCGACTCCGACATCCTGGGCGCGCTGCTGGCGCACGGCGCCGCCGACTCCACGTTGGAGCAGGCCGCGC
TGGACCTGCTGCCGACGGTGCGCGGCGCCTTCTGTCTGACCTTCATGGACGAGAACACGCTCTATGCGTGCCGCGATCCG
CATGGGGTGCGGCCACTTTCGCTGGGACGGCTGGACCGCGGCTGGGTGGTGGCCTCCGAGACCGCCGCGCTCGACATCGT
CGGCGCCTCGTTCGTGCGCGACATCGAACCGGGCGAACTGCTGGCGATCGACGCCGACGGGGTGCGCTCCACCCGGTTCG
CCAATCCCACCCCCAAGGGCTGCGTCTTCGAGTACGTCTATTTGGCGAGACCGGACAGCACCATCGCCGGCCGGTCCGTG
CACGCCACCCGGGTCGAGATCGGCCGGCGGCTGGCCCGGGAACGCCCCGTCGAGGCCGACCTGGTGATCGGGGTGCCCGA
GTCGGGCACCCCCGCCGCCGTCGGCTACGCCCAGGAATCCGGCATCCCCTACGGGCAGGGGCTGATGAAGAACGCCTACG
TGGGGCGCACCTTCATCCAGCCGTCGCAGACCATCCGTCAACTCGGTATCCGGCTCAAGCTCAACCCACTTCGCGAGGTG
ATCCGCGGCAAACGGCTCATCGTCGTCGACGACTCCATCGTGCGCGGCAACACTCAGCGGGCGCTGCTGCGGATGCTGCG
CGAGGCCGGCGCCGTCGAGGTGCACGTGCGCATCGCGTCACCGCCGGTCAAATGGCCCTGCTTCTACGGCATCGACTTCC
CGTCGCCGGCCGAACTGATCGCCAACGCGGTCGAGGACAAACACGAGATGCTCGAGGCGGTGCGGCACGCCATCGGCGCC
GACTCGCTGGGCTACATCTCGCTGCGTGGCCTGATCGCAGCCTCCGAGCAGCCGGCGTCGCGGCTGTGCACCGCCTGCTT
CGACGGCCAGTACCCGATCGAACTGCCCGGCGAGACGGCGTTGGGCAAGAACGTCATCGAGCACATGCTCGCCAACGCGG
CCCGGGGCGCCGGGCTCGACGACCTGGCGGCCGACGAAGTGCCCGTCGTCCACTGA

Upstream 100 bases:

>100_bases
TCGCCGACGCCAGGCGCACCGGTGCGCTGCGCCTTTCCGGCTCCCGCGCCGGTGAGATCGAACACTGCCTGCCCCTGTTC
GACGTGGACCGAACATAACC

Downstream 100 bases:

>100_bases
GCGGCTCGTTCGCCGACGTTTGATACCGGCGGTCAGCGCCGACCGGTAGCCTTTATCGCGATGACGGATCCCGGAAAAAG
CCCCGGACGCCACTCGGGCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MKCRFTPAGRDFAGQHARRIRRRGRAARRLRYVTNRSPGSRPIVTVAEPEQDFNAPREECGVFGVWAPGEEVAKLTYYGL
YALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALG
HNGNLVNTAELAARARDAGLIAKRAPAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDP
HGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSV
HATRVEIGRRLARERPVEADLVIGVPESGTPAAVGYAQESGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREV
IRGKRLIVVDDSIVRGNTQRALLRMLREAGAVEVHVRIASPPVKWPCFYGIDFPSPAELIANAVEDKHEMLEAVRHAIGA
DSLGYISLRGLIAASEQPASRLCTACFDGQYPIELPGETALGKNVIEHMLANAARGAGLDDLAADEVPVVH

Sequences:

>Translated_551_residues
MKCRFTPAGRDFAGQHARRIRRRGRAARRLRYVTNRSPGSRPIVTVAEPEQDFNAPREECGVFGVWAPGEEVAKLTYYGL
YALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALG
HNGNLVNTAELAARARDAGLIAKRAPAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDP
HGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSV
HATRVEIGRRLARERPVEADLVIGVPESGTPAAVGYAQESGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREV
IRGKRLIVVDDSIVRGNTQRALLRMLREAGAVEVHVRIASPPVKWPCFYGIDFPSPAELIANAVEDKHEMLEAVRHAIGA
DSLGYISLRGLIAASEQPASRLCTACFDGQYPIELPGETALGKNVIEHMLANAARGAGLDDLAADEVPVVH
>Mature_551_residues
MKCRFTPAGRDFAGQHARRIRRRGRAARRLRYVTNRSPGSRPIVTVAEPEQDFNAPREECGVFGVWAPGEEVAKLTYYGL
YALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALG
HNGNLVNTAELAARARDAGLIAKRAPAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDP
HGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSV
HATRVEIGRRLARERPVEADLVIGVPESGTPAAVGYAQESGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREV
IRGKRLIVVDDSIVRGNTQRALLRMLREAGAVEVHVRIASPPVKWPCFYGIDFPSPAELIANAVEDKHEMLEAVRHAIGA
DSLGYISLRGLIAASEQPASRLCTACFDGQYPIELPGETALGKNVIEHMLANAARGAGLDDLAADEVPVVH

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=40.655105973025, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=479, Percent_Identity=40.5010438413361, Blast_Score=298, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17554892, Length=491, Percent_Identity=38.9002036659878, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=33.7474120082816, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24659598, Length=504, Percent_Identity=40.0793650793651, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI28573187, Length=530, Percent_Identity=40.9433962264151, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24659604, Length=479, Percent_Identity=40.5010438413361, Blast_Score=348, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 59285; Mature: 59285

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCRFTPAGRDFAGQHARRIRRRGRAARRLRYVTNRSPGSRPIVTVAEPEQDFNAPREEC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHC
GVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLA
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHH
AMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALGHNGNLVNTAELAARARDAGL
HHCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCCCCEEECCCCCEEEHHHHHHHHHHCCC
IAKRAPAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDP
EEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCC
HGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKG
CCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCC
CVFEYVYLARPDSTIAGRSVHATRVEIGRRLARERPVEADLVIGVPESGTPAAVGYAQES
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHC
GIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLIVVDDSIVRGNTQR
CCCCCCHHHHCCCCCCEECCHHHHHHHHCCEEEEHHHHHHHCCCEEEEECCHHHCCCHHH
ALLRMLREAGAVEVHVRIASPPVKWPCFYGIDFPSPAELIANAVEDKHEMLEAVRHAIGA
HHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
DSLGYISLRGLIAASEQPASRLCTACFDGQYPIELPGETALGKNVIEHMLANAARGAGLD
CCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCC
DLAADEVPVVH
CCCCCCCCCCC
>Mature Secondary Structure
MKCRFTPAGRDFAGQHARRIRRRGRAARRLRYVTNRSPGSRPIVTVAEPEQDFNAPREEC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHC
GVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLA
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHH
AMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALGHNGNLVNTAELAARARDAGL
HHCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCCCCEEECCCCCEEEHHHHHHHHHHCCC
IAKRAPAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDP
EEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCC
HGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKG
CCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCC
CVFEYVYLARPDSTIAGRSVHATRVEIGRRLARERPVEADLVIGVPESGTPAAVGYAQES
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHC
GIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLIVVDDSIVRGNTQR
CCCCCCHHHHCCCCCCEECCHHHHHHHHCCEEEEHHHHHHHCCCEEEEECCHHHCCCHHH
ALLRMLREAGAVEVHVRIASPPVKWPCFYGIDFPSPAELIANAVEDKHEMLEAVRHAIGA
HHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
DSLGYISLRGLIAASEQPASRLCTACFDGQYPIELPGETALGKNVIEHMLANAARGAGLD
CCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCC
DLAADEVPVVH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]